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OPENSEQ.org

GCSH - Glycine cleavage system H protein
UniProt: P0A6T9 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10371
Length: 129 (128)
Sequences: 1850
Seq/Len: 14.45

GCSH
Paralog alert: 0.14 [within 20: 0.01] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
118_D 121_A 4.56 1.00
95_E 98_A 3.99 1.00
77_E 106_K 3.965 1.00
58_C 103_F 3.194 1.00
80_A 104_K 2.658 1.00
26_T 104_K 2.637 1.00
75_S 112_E 2.512 1.00
31_E 34_Q 2.408 1.00
111_S 114_E 2.277 1.00
52_V 72_A 1.995 1.00
51_T 77_E 1.914 1.00
109_D 112_E 1.908 1.00
51_T 108_S 1.884 1.00
117_L 125_L 1.871 1.00
88_S 91_L 1.802 1.00
95_E 99_G 1.801 1.00
92_V 102_I 1.781 1.00
25_Y 107_A 1.73 1.00
53_S 56_D 1.717 1.00
58_C 70_I 1.621 1.00
60_V 67_A 1.614 1.00
24_T 104_K 1.613 1.00
31_E 35_E 1.611 1.00
54_A 115_S 1.605 1.00
22_D 106_K 1.556 1.00
85_L 102_I 1.542 1.00
59_A 70_I 1.53 1.00
84_A 88_S 1.524 1.00
17_L 25_Y 1.5 1.00
19_K 25_Y 1.497 1.00
123_E 127_E 1.496 1.00
8_L 97_Y 1.48 1.00
74_V 107_A 1.469 1.00
91_L 100_G 1.466 1.00
119_A 123_E 1.443 1.00
8_L 18_R 1.44 1.00
12_K 125_L 1.41 1.00
53_S 75_S 1.369 0.99
34_Q 93_N 1.351 0.99
49_G 77_E 1.346 0.99
50_A 56_D 1.299 0.99
60_V 64_V 1.296 0.99
15_E 64_V 1.27 0.99
47_E 50_A 1.266 0.99
18_R 26_T 1.244 0.98
24_T 106_K 1.242 0.98
41_V 64_V 1.21 0.98
88_S 94_S 1.205 0.98
45_L 81_V 1.197 0.98
124_A 127_E 1.197 0.98
83_D 87_D 1.186 0.98
57_D 69_D 1.179 0.98
52_V 58_C 1.168 0.97
6_A 120_T 1.15 0.97
84_A 99_G 1.15 0.97
80_A 83_D 1.136 0.97
84_A 91_L 1.08 0.95
19_K 113_L 1.079 0.95
110_E 114_E 1.067 0.95
48_V 81_V 1.06 0.95
84_A 87_D 1.047 0.94
45_L 102_I 1.032 0.94
32_H 36_L 1.029 0.94
19_K 110_E 1.028 0.94
74_V 105_I 1.007 0.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3hgbA10.99221000.069Contact Map0.65
3mxuA10.9691000.13Contact Map0.688
3a7lA10.99221000.157Contact Map0.726
1hpcA20.98451000.162Contact Map0.818
3tzuA40.9691000.171Contact Map0.787
3klrA10.95351000.192Contact Map0.814
3wdnA10.95351000.192Contact Map0.806
1onlA30.98451000.211Contact Map0.851
1zkoA20.99221000.224Contact Map0.854
1gjxA10.627996.50.839Contact Map0.608

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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