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OPENSEQ.org
SPEE - Spermidine synthase
UniProt: P09158 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10963
Length: 288 (282)
Sequences: 1211
Seq/Len: 4.29
SPEE
Paralog alert: 0.13 [within 20: 0.01] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
79_K 102_E 6.04 1.00
265_N 277_Q 3.77 1.00
28_E 112_G 3.446 1.00
83_I 92_L 3.43 1.00
23_N 39_E 3.285 1.00
37_I 116_F 3.284 1.00
105_T 152_F 3.144 1.00
114_V 138_I 3.087 1.00
103_S 135_K 2.958 1.00
22_D 39_E 2.95 1.00
264_Y 269_H 2.872 1.00
80_H 103_S 2.814 1.00
111_A 115_S 2.81 1.00
179_K 238_D 2.613 1.00
83_I 95_V 2.537 1.00
92_L 104_I 2.502 1.00
172_S 176_E 2.469 1.00
92_L 134_F 2.457 1.00
84_I 141_G 2.446 1.00
22_D 41_A 2.349 1.00
26_Y 112_G 2.231 1.00
30_T 35_L 2.198 1.00
29_K 34_D 2.149 1.00
112_G 115_S 2.142 1.00
92_L 129_Y 2.085 1.00
39_E 45_R 2.065 1.00
75_H 78_A 2.033 1.00
182_L 186_G 2.031 1.00
27_H 34_D 2.001 1.00
96_T 104_I 1.962 1.00
104_I 134_F 1.922 1.00
143_N 147_Q 1.92 1.00
105_T 135_K 1.913 1.00
57_E 123_N 1.912 1.00
95_V 154_V 1.888 1.00
68_T 72_L 1.882 1.00
83_I 134_F 1.88 1.00
149_S 180_R 1.856 1.00
20_A 41_A 1.833 1.00
185_G 239_N 1.811 1.00
72_L 154_V 1.738 0.99
106_M 136_L 1.674 0.99
145_V 177_G 1.668 0.99
31_D 111_A 1.622 0.99
105_T 137_V 1.617 0.99
30_T 37_I 1.585 0.99
72_L 95_V 1.575 0.99
38_F 48_A 1.574 0.99
12_H 15_F 1.55 0.98
148_T 151_T 1.548 0.98
172_S 211_H 1.539 0.98
209_L 236_A 1.504 0.98
118_R 130_D 1.487 0.98
81_V 95_V 1.472 0.98
265_N 268_I 1.465 0.97
96_T 133_R 1.456 0.97
277_Q 280_Q 1.447 0.97
75_H 154_V 1.443 0.97
189_V 219_Y 1.441 0.97
95_V 101_V 1.437 0.97
78_A 100_N 1.426 0.97
81_V 101_V 1.419 0.97
271_A 274_A 1.416 0.97
95_V 104_I 1.411 0.97
209_L 234_A 1.41 0.97
150_Q 184_P 1.403 0.96
178_C 188_F 1.392 0.96
155_I 178_C 1.389 0.96
128_S 131_D 1.381 0.96
143_N 146_N 1.369 0.96
80_H 105_T 1.364 0.96
146_N 177_G 1.359 0.96
142_V 168_S 1.355 0.95
145_V 181_C 1.351 0.95
267_A 270_T 1.35 0.95
101_V 154_V 1.345 0.95
27_H 36_I 1.285 0.93
214_S 238_D 1.278 0.93
246_S 249_I 1.277 0.93
56_T 225_T 1.274 0.93
24_V 27_H 1.255 0.92
127_G 130_D 1.246 0.92
59_D 225_T 1.245 0.92
273_F 276_P 1.229 0.91
185_G 240_D 1.227 0.91
68_T 91_M 1.219 0.91
192_N 234_A 1.211 0.91
157_S 174_F 1.21 0.90
265_N 280_Q 1.19 0.90
155_I 182_L 1.183 0.89
70_V 219_Y 1.182 0.89
9_E 18_Y 1.179 0.89
184_P 238_D 1.173 0.89
55_T 88_D 1.168 0.88
114_V 136_L 1.164 0.88
267_A 280_Q 1.159 0.88
80_H 183_N 1.157 0.88
111_A 138_I 1.152 0.87
60_E 93_R 1.134 0.86
68_T 156_I 1.12 0.85
115_S 119_Q 1.119 0.85
62_I 221_A 1.109 0.85
95_V 156_I 1.086 0.83
73_L 98_H 1.084 0.83
167_E 171_T 1.079 0.82
9_E 16_G 1.078 0.82
129_Y 134_F 1.078 0.82
82_L 152_F 1.078 0.82
69_H 98_H 1.072 0.82
262_R 281_D 1.07 0.82
72_L 101_V 1.06 0.81
26_Y 116_F 1.051 0.80
145_V 178_C 1.051 0.80
64_H 91_M 1.026 0.78
94_E 97_R 1.026 0.78
205_S 208_K 1.012 0.76
40_N 46_V 1.01 0.76
97_R 261_C 1.009 0.76
154_V 187_I 1.007 0.76
71_P 154_V 1.001 0.75
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1xj5A40.99651000.379Contact Map0.857
1inlA40.97571000.385Contact Map0.835
3adnA811000.385Contact Map0.726
2o07A20.98261000.388Contact Map0.793
2i7cA30.97221000.395Contact Map0.877
2pt6A30.97221000.395Contact Map0.874
1uirA20.99311000.397Contact Map0.778
2b2cA20.97571000.402Contact Map0.807
2cmgA20.89931000.41Contact Map0.748
1mjfA20.94441000.412Contact Map0.836
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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