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OPENSEQ.org
T1MK - Type I restriction enzyme EcoKI M protein
UniProt: P08957 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10458
Length: 529 (482)
Sequences: 1324
Seq/Len: 2.75
T1MK
Paralog alert: 0.27 [within 20: 0.02] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
453_E 457_T 3.958 1.00
375_V 455_I 3.872 1.00
326_R 330_D 3.77 1.00
159_I 307_A 3.477 1.00
394_T 397_H 3.166 1.00
448_R 454_W 3.025 1.00
159_I 264_A 2.959 1.00
399_Q 403_R 2.901 1.00
334_L 357_F 2.77 1.00
323_D 326_R 2.628 1.00
330_D 456_R 2.573 1.00
157_K 395_D 2.51 1.00
453_E 456_R 2.342 1.00
334_L 355_L 2.328 1.00
300_L 306_A 2.263 1.00
417_E 448_R 2.18 1.00
293_M 310_V 2.16 1.00
53_G 58_D 2.146 1.00
296_I 308_V 2.131 1.00
343_G 349_G 2.071 1.00
161_H 188_Y 2.056 1.00
261_H 301_H 2.053 1.00
326_R 456_R 2.04 1.00
327_D 331_K 2.03 1.00
451_S 454_W 2.005 1.00
417_E 447_W 1.997 1.00
404_V 446_R 1.989 1.00
337_I 355_L 1.96 0.99
169_E 305_R 1.929 0.99
454_W 457_T 1.917 0.99
171_V 185_A 1.914 0.99
495_Q 499_E 1.901 0.99
296_I 306_A 1.883 0.99
60_K 111_D 1.867 0.99
329_M 375_V 1.86 0.99
338_L 356_F 1.788 0.99
161_H 399_Q 1.786 0.99
374_D 451_S 1.764 0.99
12_K 15_D 1.759 0.98
38_K 42_E 1.729 0.98
452_R 456_R 1.722 0.98
69_Q 73_K 1.704 0.98
336_T 405_Y 1.702 0.98
59_L 107_V 1.693 0.98
499_E 502_A 1.684 0.98
308_V 357_F 1.678 0.98
380_L 401_F 1.675 0.98
169_E 262_I 1.667 0.98
383_N 397_H 1.663 0.98
312_D 339_R 1.652 0.97
335_H 415_R 1.65 0.97
16_N 105_A 1.642 0.97
491_G 495_Q 1.64 0.97
338_L 401_F 1.639 0.97
311_P 316_F 1.639 0.97
456_R 461_D 1.635 0.97
172_Q 249_T 1.616 0.97
15_D 18_R 1.578 0.96
337_I 375_V 1.571 0.96
279_R 282_V 1.567 0.96
374_D 449_K 1.562 0.96
400_P 404_V 1.548 0.96
491_G 494_V 1.541 0.96
315_L 337_I 1.534 0.96
297_I 332_C 1.531 0.96
480_P 484_V 1.521 0.95
487_A 501_D 1.509 0.95
336_T 356_F 1.496 0.95
35_L 38_K 1.473 0.94
334_L 337_I 1.468 0.94
8_A 12_K 1.459 0.94
24_Y 28_V 1.42 0.93
161_H 402_E 1.417 0.93
339_R 465_I 1.416 0.92
307_A 354_V 1.414 0.92
265_T 296_I 1.412 0.92
216_E 221_T 1.409 0.92
338_L 354_V 1.407 0.92
322_T 326_R 1.402 0.92
328_L 333_H 1.401 0.92
261_H 303_G 1.396 0.92
502_A 506_E 1.393 0.92
335_H 358_T 1.389 0.92
335_H 405_Y 1.383 0.91
496_A 499_E 1.368 0.91
478_S 481_E 1.367 0.91
262_I 305_R 1.365 0.91
337_I 377_V 1.364 0.91
161_H 191_S 1.364 0.91
330_D 461_D 1.345 0.90
162_L 356_F 1.338 0.89
176_A 225_A 1.337 0.89
494_V 498_S 1.327 0.89
293_M 357_F 1.318 0.88
293_M 308_V 1.285 0.87
158_T 340_L 1.284 0.87
315_L 353_N 1.283 0.87
312_D 465_I 1.281 0.86
297_I 328_L 1.272 0.86
162_L 338_L 1.271 0.86
136_Q 347_A 1.265 0.86
307_A 356_F 1.26 0.85
25_Q 29_N 1.254 0.85
399_Q 453_E 1.254 0.85
175_A 354_V 1.251 0.85
393_F 401_F 1.247 0.84
23_S 26_N 1.244 0.84
491_G 505_R 1.24 0.84
154_P 392_P 1.24 0.84
418_G 447_W 1.231 0.83
332_C 373_D 1.227 0.83
482_P 485_L 1.225 0.83
54_Y 77_H 1.216 0.82
9_K 112_S 1.216 0.82
294_Q 324_I 1.212 0.82
502_A 505_R 1.21 0.82
164_K 406_G 1.208 0.82
142_T 347_A 1.203 0.81
487_A 498_S 1.201 0.81
136_Q 140_N 1.195 0.81
490_M 504_M 1.195 0.81
176_A 244_I 1.195 0.81
492_E 495_Q 1.194 0.81
214_G 244_I 1.19 0.80
499_E 503_L 1.189 0.80
9_K 12_K 1.188 0.80
25_Q 28_V 1.186 0.80
485_L 489_A 1.184 0.80
292_F 314_V 1.183 0.80
163_L 262_I 1.182 0.80
148_Q 218_V 1.176 0.79
222_R 226_L 1.175 0.79
479_L 486_A 1.175 0.79
310_V 353_N 1.168 0.79
305_R 358_T 1.165 0.78
378_Y 405_Y 1.162 0.78
101_K 105_A 1.161 0.78
312_D 316_F 1.158 0.78
373_D 451_S 1.154 0.78
489_A 493_L 1.154 0.78
263_V 301_H 1.154 0.78
484_V 488_E 1.152 0.77
156_I 180_G 1.152 0.77
492_E 496_A 1.149 0.77
26_N 96_T 1.142 0.76
316_F 465_I 1.137 0.76
498_S 502_A 1.137 0.76
487_A 491_G 1.135 0.76
483_D 487_A 1.132 0.76
495_Q 498_S 1.131 0.76
484_V 495_Q 1.127 0.75
36_F 39_M 1.126 0.75
290_L 293_M 1.12 0.75
496_A 500_L 1.119 0.74
484_V 487_A 1.119 0.74
490_M 494_V 1.119 0.74
179_A 225_A 1.118 0.74
383_N 400_P 1.115 0.74
156_I 181_F 1.114 0.74
175_A 292_F 1.111 0.74
67_Q 175_A 1.11 0.74
490_M 497_L 1.109 0.73
158_T 354_V 1.108 0.73
168_R 261_H 1.107 0.73
165_P 168_R 1.106 0.73
387_F 390_R 1.103 0.73
314_V 353_N 1.097 0.72
488_E 492_E 1.094 0.72
158_T 162_L 1.094 0.72
492_E 502_A 1.093 0.72
492_E 504_M 1.093 0.72
164_K 188_Y 1.093 0.72
189_V 192_Q 1.092 0.72
374_D 405_Y 1.09 0.72
396_E 399_Q 1.09 0.72
229_C 307_A 1.087 0.71
129_D 346_Y 1.086 0.71
500_L 504_M 1.079 0.71
351_K 464_D 1.078 0.70
10_L 130_M 1.077 0.70
503_L 508_G 1.073 0.70
316_F 339_R 1.072 0.70
452_R 461_D 1.068 0.69
493_L 497_L 1.067 0.69
488_E 491_G 1.067 0.69
297_I 333_H 1.064 0.69
497_L 500_L 1.064 0.69
490_M 493_L 1.063 0.69
62_R 70_F 1.063 0.69
22_V 25_Q 1.063 0.69
496_A 503_L 1.057 0.68
315_L 343_G 1.056 0.68
265_T 269_F 1.054 0.68
172_Q 299_T 1.053 0.68
129_D 232_H 1.053 0.68
11_W 137_K 1.048 0.67
142_T 146_A 1.046 0.67
163_L 264_A 1.044 0.67
157_K 161_H 1.037 0.66
33_S 36_F 1.037 0.66
122_K 237_N 1.036 0.66
453_E 475_D 1.036 0.66
311_P 339_R 1.034 0.66
487_A 502_A 1.033 0.66
229_C 377_V 1.033 0.66
58_D 61_S 1.026 0.65
125_D 138_N 1.025 0.65
165_P 180_G 1.022 0.64
336_T 376_W 1.022 0.64
501_D 504_M 1.02 0.64
495_Q 502_A 1.019 0.64
403_R 414_P 1.018 0.64
403_R 498_S 1.017 0.64
484_V 491_G 1.017 0.64
250_L 292_F 1.016 0.64
128_G 133_G 1.015 0.64
480_P 488_E 1.014 0.64
342_T 349_G 1.013 0.63
483_D 496_A 1.011 0.63
83_K 94_S 1.009 0.63
157_K 184_E 1.003 0.62
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2ar0A20.99431000.313Contact Map0.598
3lkdA20.87521000.384Contact Map0.702
3ufbA10.86011000.393Contact Map0.618
2okcA20.81661000.403Contact Map0.621
3khkA20.8451000.419Contact Map0.729
3s1sA10.87331000.63Contact Map0.379
2f8lA10.621000.688Contact Map0.554
2ih2A20.58411000.805Contact Map0.592
3v97A20.8111000.817Contact Map0.28
3lduA10.593699.80.882Contact Map0.362
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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