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OPENSEQ.org
SYFA - Phenylalanine--tRNA ligase alpha subunit
UniProt: P08312 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10709
Length: 327 (327)
Sequences: 1407
Seq/Len: 4.30
SYFA
Paralog alert: 0.01 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
216_T 289_V 6.677 1.00
135_D 138_H 3.947 1.00
212_L 275_V 3.552 1.00
251_T 254_S 3.482 1.00
20_D 23_A 3.372 1.00
278_N 281_R 3.3 1.00
281_R 287_P 2.696 1.00
137_Y 142_A 2.687 1.00
255_A 272_C 2.541 1.00
136_D 141_D 2.529 1.00
211_G 295_F 2.494 1.00
12_K 70_A 2.469 1.00
125_F 226_T 2.441 1.00
116_I 209_M 2.427 1.00
269_V 302_L 2.392 1.00
227_L 270_L 2.33 1.00
213_I 218_I 2.27 1.00
261_G 265_K 2.225 1.00
220_F 255_A 2.199 1.00
14_A 30_E 2.195 1.00
199_D 202_H 2.19 1.00
228_H 241_I 2.139 1.00
138_H 179_K 2.126 1.00
285_I 290_Y 2.105 1.00
49_P 52_E 2.087 1.00
74_R 77_E 2.078 1.00
154_D 246_S 2.063 1.00
115_R 306_R 2.03 1.00
281_R 288_E 2.017 1.00
240_Q 260_M 1.938 1.00
69_Q 73_A 1.908 1.00
178_M 185_I 1.9 1.00
44_T 47_E 1.898 1.00
128_A 187_I 1.875 1.00
141_D 147_G 1.847 1.00
175_I 283_V 1.832 1.00
75_K 79_E 1.791 1.00
81_A 85_A 1.741 0.99
177_T 181_Q 1.722 0.99
84_N 88_A 1.671 0.99
85_A 89_A 1.644 0.99
19_S 23_A 1.635 0.99
41_Q 60_I 1.592 0.99
139_N 168_T 1.577 0.99
76_A 80_S 1.565 0.99
116_I 299_M 1.548 0.98
58_A 62_E 1.517 0.98
68_Q 72_N 1.506 0.98
15_I 27_V 1.503 0.98
4_L 63_A 1.5 0.98
21_V 78_L 1.487 0.98
10_S 14_A 1.469 0.98
214_V 280_L 1.464 0.97
222_N 225_G 1.444 0.97
8_V 66_Q 1.436 0.97
257_V 270_L 1.426 0.97
205_M 316_F 1.425 0.97
228_H 232_R 1.411 0.97
15_I 18_A 1.405 0.97
25_D 28_R 1.405 0.97
6_E 10_S 1.396 0.96
106_L 114_D 1.392 0.96
113_I 299_M 1.383 0.96
25_D 75_K 1.38 0.96
297_M 302_L 1.367 0.96
106_L 110_T 1.348 0.95
145_I 278_N 1.343 0.95
137_Y 179_K 1.343 0.95
30_E 37_H 1.335 0.95
22_A 26_N 1.318 0.95
127_V 188_I 1.315 0.94
80_S 84_N 1.315 0.94
200_Q 203_T 1.314 0.94
28_R 75_K 1.31 0.94
77_E 81_A 1.31 0.94
203_T 316_F 1.294 0.94
230_F 234_F 1.291 0.94
197_D 203_T 1.29 0.94
145_I 159_W 1.285 0.94
119_F 233_N 1.281 0.93
142_A 175_I 1.263 0.93
21_V 79_E 1.261 0.93
136_D 155_H 1.245 0.92
50_P 53_R 1.235 0.92
18_A 27_V 1.23 0.91
280_L 285_I 1.229 0.91
269_V 305_L 1.223 0.91
322_F 326_F 1.22 0.91
114_D 117_E 1.218 0.91
128_A 189_A 1.216 0.91
181_Q 185_I 1.209 0.90
36_G 39_T 1.206 0.90
13_A 17_Q 1.201 0.90
188_I 209_M 1.2 0.90
56_A 60_I 1.197 0.90
46_R 53_R 1.193 0.90
82_A 86_R 1.189 0.89
88_A 91_T 1.188 0.89
42_M 60_I 1.187 0.89
239_L 260_M 1.184 0.89
86_R 90_E 1.17 0.88
42_M 64_K 1.169 0.88
38_L 67_V 1.166 0.88
197_D 201_T 1.161 0.88
134_E 172_G 1.16 0.88
154_D 247_Y 1.143 0.87
260_M 263_N 1.142 0.87
300_E 315_F 1.139 0.87
178_M 285_I 1.137 0.86
108_P 111_R 1.134 0.86
72_N 76_A 1.133 0.86
11_A 67_V 1.132 0.86
226_T 293_F 1.123 0.86
60_I 63_A 1.109 0.85
213_I 226_T 1.109 0.85
54_P 58_A 1.107 0.84
9_A 13_A 1.103 0.84
82_A 85_A 1.103 0.84
199_D 204_P 1.101 0.84
174_Q 275_V 1.095 0.84
126_T 181_Q 1.092 0.83
85_A 88_A 1.087 0.83
234_F 302_L 1.083 0.83
241_I 257_V 1.082 0.83
69_Q 72_N 1.078 0.82
65_E 69_Q 1.077 0.82
137_Y 282_N 1.073 0.82
142_A 283_V 1.071 0.82
90_E 93_D 1.061 0.81
67_V 71_L 1.06 0.81
197_D 204_P 1.057 0.81
244_R 258_D 1.057 0.81
23_A 26_N 1.056 0.80
127_V 190_P 1.054 0.80
280_L 287_P 1.053 0.80
122_E 233_N 1.053 0.80
9_A 12_K 1.052 0.80
61_N 64_K 1.048 0.80
312_L 316_F 1.045 0.79
218_I 226_T 1.043 0.79
286_D 290_Y 1.041 0.79
9_A 69_Q 1.04 0.79
184_P 215_D 1.037 0.79
50_P 54_P 1.035 0.79
215_D 290_Y 1.035 0.79
78_L 82_A 1.031 0.78
48_L 56_A 1.031 0.78
62_E 66_Q 1.03 0.78
87_L 90_E 1.028 0.78
154_D 169_Q 1.026 0.78
309_V 315_F 1.026 0.78
231_L 257_V 1.022 0.77
116_I 188_I 1.018 0.77
5_A 8_V 1.018 0.77
39_T 43_T 1.017 0.77
52_E 55_A 1.015 0.77
83_L 86_R 1.015 0.77
177_T 183_P 1.012 0.76
112_T 115_R 1.011 0.76
134_E 176_R 1.008 0.76
131_P 176_R 1.007 0.76
312_L 315_F 1.007 0.76
12_K 16_S 1.006 0.76
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1b7yA10.98171000.339Contact Map0.6
3pcoA20.99691000.391Contact Map0.566
3l4gA80.9451000.59Contact Map0.621
3cmqA10.80431000.591Contact Map0.618
2rhqA10.83491000.616Contact Map0.759
2du3A20.78291000.623Contact Map0.605
2du7A40.81351000.648Contact Map0.596
2odrC10.81041000.666Contact Map0.59
2odrD10.81041000.669Contact Map0.593
2odrA10.81041000.672Contact Map0.59
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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