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OPENSEQ.org
T1SK - Type-1 restriction enzyme EcoKI specificity protein
UniProt: P05719 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10460
Length: 464 (417)
Sequences: 2317
Seq/Len: 5.56
T1SK
Paralog alert: 0.24 [within 20: 0.03] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
83_S 146_N 2.484 1.00
294_N 359_G 2.386 1.00
192_I 405_R 2.354 1.00
77_D 112_R 2.291 1.00
107_F 146_N 2.259 1.00
83_S 107_F 2.174 1.00
319_K 359_G 2.069 1.00
199_A 412_S 2.057 1.00
82_M 146_N 2.03 1.00
149_P 153_D 2.019 1.00
294_N 319_K 2 1.00
416_K 421_E 1.935 1.00
84_S 145_N 1.906 1.00
104_F 107_F 1.862 1.00
196_F 409_L 1.861 1.00
83_S 144_I 1.846 1.00
292_R 296_S 1.805 1.00
237_N 240_S 1.774 1.00
82_M 106_A 1.701 1.00
202_G 415_A 1.682 1.00
107_F 144_I 1.675 1.00
294_N 357_Q 1.661 1.00
293_Y 359_G 1.632 0.99
82_M 107_F 1.62 0.99
284_Q 287_D 1.615 0.99
81_A 85_G 1.602 0.99
293_Y 319_K 1.598 0.99
6_L 9_G 1.597 0.99
82_M 109_G 1.596 0.99
287_D 324_R 1.593 0.99
84_S 144_I 1.59 0.99
195_R 408_N 1.581 0.99
316_Y 319_K 1.564 0.99
362_G 366_K 1.56 0.99
226_L 230_S 1.547 0.99
118_F 166_K 1.545 0.99
293_Y 318_D 1.526 0.99
295_G 358_K 1.51 0.99
45_R 48_N 1.491 0.99
377_E 381_I 1.459 0.99
185_R 398_Q 1.454 0.98
381_I 385_V 1.451 0.98
81_A 84_S 1.45 0.98
192_I 196_F 1.45 0.98
193_L 198_Q 1.436 0.98
104_F 110_V 1.418 0.98
46_A 83_S 1.417 0.98
319_K 357_Q 1.417 0.98
317_P 320_L 1.412 0.98
295_G 357_Q 1.409 0.98
46_A 81_A 1.407 0.98
196_F 200_V 1.401 0.98
316_Y 322_R 1.391 0.98
293_Y 321_I 1.384 0.98
409_L 412_S 1.381 0.98
182_T 395_I 1.379 0.98
416_K 422_L 1.372 0.98
226_L 372_L 1.367 0.97
45_R 146_N 1.363 0.97
197_R 201_L 1.36 0.97
240_S 318_D 1.355 0.97
82_M 148_K 1.348 0.97
418_F 422_L 1.346 0.97
293_Y 357_Q 1.343 0.97
375_V 378_Q 1.33 0.97
318_D 321_I 1.328 0.97
255_I 294_N 1.327 0.97
189_I 193_L 1.31 0.96
23_R 83_S 1.308 0.96
44_I 48_N 1.306 0.96
24_G 27_Y 1.295 0.96
332_E 386_E 1.294 0.96
414_L 418_F 1.289 0.96
417_A 422_L 1.285 0.96
46_A 146_N 1.283 0.96
330_L 379_A 1.282 0.96
43_L 48_N 1.281 0.96
85_G 144_I 1.278 0.96
176_L 179_V 1.276 0.96
405_R 409_L 1.275 0.96
105_G 109_G 1.268 0.96
182_T 186_F 1.267 0.96
23_R 26_T 1.26 0.95
146_N 149_P 1.259 0.95
292_R 295_G 1.241 0.95
412_S 416_K 1.236 0.95
240_S 243_P 1.234 0.95
84_S 143_N 1.234 0.95
81_A 144_I 1.231 0.95
84_S 107_F 1.227 0.94
106_A 109_G 1.218 0.94
293_Y 317_P 1.214 0.94
237_N 293_Y 1.213 0.94
410_T 415_A 1.213 0.94
82_M 144_I 1.212 0.94
14_P 156_N 1.209 0.94
227_N 369_V 1.206 0.94
27_Y 43_L 1.206 0.94
21_L 24_G 1.206 0.94
16_S 153_D 1.203 0.94
77_D 110_V 1.203 0.94
254_R 257_S 1.2 0.94
293_Y 361_S 1.199 0.93
105_G 108_C 1.195 0.93
201_L 204_A 1.195 0.93
316_Y 320_L 1.194 0.93
294_N 318_D 1.19 0.93
199_A 202_G 1.188 0.93
255_I 295_G 1.186 0.93
26_T 29_K 1.183 0.93
229_E 366_K 1.18 0.93
361_S 364_D 1.176 0.93
104_F 108_C 1.17 0.92
321_I 365_I 1.17 0.92
359_G 362_G 1.162 0.92
117_I 122_I 1.161 0.92
197_R 410_T 1.158 0.92
79_V 108_C 1.156 0.92
254_R 359_G 1.152 0.92
84_S 105_G 1.147 0.91
182_T 189_I 1.147 0.91
81_A 92_K 1.142 0.91
203_G 416_K 1.141 0.91
237_N 359_G 1.14 0.91
14_P 154_L 1.134 0.91
356_G 359_G 1.132 0.91
46_A 82_M 1.132 0.91
255_I 292_R 1.132 0.91
382_V 385_V 1.131 0.91
169_A 172_L 1.128 0.90
95_H 120_G 1.127 0.90
85_G 107_F 1.125 0.90
187_E 191_Q 1.124 0.90
205_V 418_F 1.117 0.90
45_R 107_F 1.116 0.90
201_L 206_N 1.115 0.90
253_L 257_S 1.113 0.90
182_T 185_R 1.106 0.89
186_F 399_V 1.09 0.88
255_I 359_G 1.089 0.88
295_G 356_G 1.089 0.88
235_L 238_G 1.088 0.88
178_Q 182_T 1.086 0.88
13_A 17_T 1.078 0.88
290_F 295_G 1.078 0.88
415_A 418_F 1.077 0.88
287_D 322_R 1.076 0.87
26_T 106_A 1.074 0.87
23_R 146_N 1.068 0.87
106_A 146_N 1.068 0.87
253_L 258_V 1.066 0.87
83_S 141_G 1.064 0.87
83_S 104_F 1.064 0.87
167_I 380_E 1.064 0.87
200_V 413_I 1.061 0.86
186_F 200_V 1.06 0.86
83_S 149_P 1.059 0.86
315_L 318_D 1.058 0.86
232_L 323_A 1.058 0.86
167_I 170_E 1.054 0.86
180_D 183_K 1.052 0.86
43_L 82_M 1.051 0.86
411_Q 415_A 1.051 0.86
197_R 204_A 1.051 0.86
186_F 402_A 1.05 0.86
252_I 293_Y 1.05 0.86
182_T 196_F 1.048 0.86
171_K 413_I 1.048 0.86
105_G 143_N 1.045 0.85
194_K 396_E 1.044 0.85
204_A 417_A 1.042 0.85
83_S 105_G 1.041 0.85
84_S 90_V 1.04 0.85
202_G 206_N 1.04 0.85
82_M 152_F 1.04 0.85
81_A 145_N 1.04 0.85
293_Y 362_G 1.037 0.85
164_E 167_I 1.036 0.85
179_V 182_T 1.034 0.84
169_A 173_D 1.031 0.84
45_R 106_A 1.031 0.84
187_E 407_N 1.031 0.84
186_F 189_I 1.029 0.84
292_R 357_Q 1.029 0.84
237_N 318_D 1.029 0.84
289_L 292_R 1.028 0.84
252_I 255_I 1.027 0.84
134_K 137_S 1.025 0.84
179_V 183_K 1.021 0.83
143_N 146_N 1.021 0.83
227_N 367_S 1.02 0.83
361_S 365_I 1.02 0.83
283_L 357_Q 1.018 0.83
192_I 199_A 1.018 0.83
318_D 322_R 1.018 0.83
185_R 188_Q 1.016 0.83
295_G 319_K 1.015 0.83
109_G 149_P 1.015 0.83
46_A 85_G 1.015 0.83
144_I 148_K 1.015 0.83
296_S 300_V 1.015 0.83
237_N 294_N 1.014 0.83
25_V 82_M 1.013 0.83
197_R 202_G 1.013 0.83
255_I 357_Q 1.01 0.83
284_Q 324_R 1.01 0.83
23_R 105_G 1.008 0.82
301_G 304_G 1.008 0.82
90_V 144_I 1.008 0.82
189_I 402_A 1.007 0.82
237_N 317_P 1.007 0.82
46_A 144_I 1.004 0.82
162_L 166_K 1.002 0.82
171_K 175_L 1.002 0.82
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2y7cA10.98061000.183Contact Map0.283
1yf2A20.85131000.357Contact Map0.436
3okgA20.82761000.387Contact Map0.434
1ydxA10.78881000.436Contact Map0.325
2ih2A20.349195.70.933Contact Map0.505
3s1sA10.467721.90.968Contact Map0.338
3kxeC20.17894.70.976Contact Map0.355
2r9iA10.13794.70.976Contact Map0.26
1o5lA10.40734.70.976Contact Map0.067
1yzmA10.0974.30.977Contact Map0.601
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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