OPENSEQ.org - RS7 - ECOLI GREMLIN

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RS7 - 30S ribosomal protein S7

UniProt:	P02359	Sequence MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVYSALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMRWIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRWLSLRSFSHQAGASSKQPALGYLN	Download Alignment We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene:	EG10906
Length:	179 (156)
Sequences:	1155
Seq/Len:	7.40

RS7
Paralog alert: 0.01 [within 20: 0.00] - ratio of genomes with paralogs

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Scaled Score	Prob
20_S	63_E	3.723	1.00
30_L	43_V	3.041	1.00
56_K	64_V	2.847	1.00
2_P	7_I	2.828	1.00
106_E	110_K	2.689	1.00
54_S	125_S	2.378	1.00
22_L	97_N	2.345	1.00
23_L	47_L	2.313	1.00
69_V	104_I	2.287	1.00
125_S	128_A	2.287	1.00
136_K	140_D	2.246	1.00
45_S	117_A	2.132	1.00
51_A	58_E	2.046	1.00
126_D	131_K	1.953	1.00
70_R	96_R	1.846	1.00
9_Q	94_V	1.842	1.00
51_A	57_S	1.816	1.00
43_V	47_L	1.782	1.00
48_E	51_A	1.778	1.00
107_A	134_A	1.766	1.00
69_V	134_A	1.763	1.00
49_T	118_L	1.756	1.00
49_T	117_A	1.755	1.00
113_D	119_R	1.695	1.00
49_T	121_A	1.679	1.00
30_L	39_A	1.644	1.00
50_L	61_A	1.642	1.00
70_R	100_A	1.551	1.00
76_K	89_V	1.536	1.00
67_E	70_R	1.523	0.99
26_F	101_M	1.494	0.99
2_P	5_R	1.493	0.99
137_K	141_V	1.486	0.99
130_N	135_V	1.431	0.99
29_I	97_N	1.414	0.99
50_L	124_L	1.413	0.99
72_T	96_R	1.347	0.98
47_L	62_F	1.347	0.98
69_V	124_L	1.332	0.98
30_L	105_V	1.319	0.98
141_V	156_W	1.308	0.98
53_R	122_N	1.287	0.98
26_F	105_V	1.283	0.98
107_A	110_K	1.281	0.98
2_P	6_V	1.279	0.98
77_S	84_T	1.268	0.97
38_T	41_S	1.266	0.97
74_E	95_R	1.265	0.97
49_T	53_R	1.243	0.97
42_I	116_M	1.228	0.97
66_L	100_A	1.213	0.96
23_L	43_V	1.205	0.96
26_F	124_L	1.202	0.96
61_A	128_A	1.185	0.96
26_F	62_F	1.183	0.96
36_K	56_K	1.178	0.96
28_N	36_K	1.17	0.95
91_V	99_L	1.166	0.95
49_T	52_Q	1.155	0.95
152_A	156_W	1.15	0.95
113_D	122_N	1.118	0.94
101_M	105_V	1.114	0.94
47_L	50_L	1.089	0.93
107_A	133_T	1.083	0.92
25_K	31_M	1.081	0.92
26_F	43_V	1.077	0.92
50_L	128_A	1.077	0.92
59_L	114_K	1.06	0.91
50_L	125_S	1.056	0.91
54_S	128_A	1.054	0.91
63_E	67_E	1.053	0.91
11_K	21_E	1.05	0.91
103_W	141_V	1.038	0.90
29_I	156_W	1.037	0.90
122_N	126_D	1.035	0.90
57_S	60_E	1.028	0.89
27_V	40_E	1.024	0.89
16_P	142_H	1.002	0.88

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling

HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:

Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	P(%)	HHΔ		Acc
4kiyG	1	1	100	0.048	Contact Map	0.652
1husA	1	0.8659	100	0.221	Contact Map	0.804
1rssA	1	0.8436	100	0.241	Contact Map	0.759
3gtyS	1	0.8324	100	0.242	Contact Map	0.661
3bbnG	1	0.8659	100	0.243	Contact Map	0.464
3u5cF	1	0.8101	100	0.384	Contact Map	0.651
3zey2	1	0.8045	100	0.394	Contact Map	0.536
4bpeG	1	0.8045	100	0.399	Contact Map	0.607
3j3aF	1	0.8045	100	0.403	Contact Map	0.595
3j38F	1	0.8045	100	0.404	Contact Map	0.616

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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