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OPENSEQ.org
SYM - Methionine--tRNA ligase
UniProt: P00959 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10586
Length: 677 (672)
Sequences: 1449
Seq/Len: 2.16
SYM
Paralog alert: 0.05 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
124_T 187_D 4.222 1.00
45_E 281_Y 3.717 1.00
40_R 91_S 3.556 1.00
112_S 116_E 3.217 1.00
595_S 643_R 3.095 1.00
601_L 653_A 3.019 1.00
588_N 602_T 2.981 1.00
405_F 475_Q 2.98 1.00
470_R 473_D 2.946 1.00
575_D 578_K 2.94 1.00
590_E 600_R 2.914 1.00
584_A 603_L 2.906 1.00
82_Q 86_A 2.901 1.00
622_D 625_A 2.862 1.00
10_V 36_V 2.841 1.00
612_V 633_M 2.82 1.00
204_A 208_S 2.808 1.00
591_F 617_R 2.685 1.00
428_V 444_E 2.648 1.00
126_S 187_D 2.632 1.00
401_I 482_I 2.597 1.00
122_N 189_E 2.557 1.00
150_K 172_I 2.538 1.00
101_E 105_Q 2.52 1.00
128_L 142_V 2.512 1.00
585_L 630_H 2.473 1.00
464_V 477_I 2.441 1.00
460_A 463_V 2.417 1.00
602_T 611_N 2.351 1.00
592_V 600_R 2.334 1.00
39_Q 44_H 2.306 1.00
199_S 203_Q 2.303 1.00
34_V 361_T 2.293 1.00
420_K 424_D 2.29 1.00
588_N 627_I 2.283 1.00
596_D 643_R 2.282 1.00
130_D 133_K 2.28 1.00
201_M 311_E 2.252 1.00
408_V 524_K 2.251 1.00
394_A 485_F 2.223 1.00
68_I 76_E 2.201 1.00
117_N 314_N 2.175 1.00
452_A 484_L 2.128 1.00
444_E 447_A 2.105 0.99
541_E 545_E 2.087 0.99
589_A 601_L 2.08 0.99
502_R 529_K 2.079 0.99
176_S 179_S 2.039 0.99
130_D 176_S 2.023 0.99
277_S 280_E 2.009 0.99
613_F 642_M 2.008 0.99
131_P 184_V 1.999 0.99
581_L 632_I 1.995 0.99
454_R 458_E 1.989 0.99
119_F 198_F 1.986 0.99
36_V 40_R 1.985 0.99
32_A 293_F 1.979 0.99
89_N 498_K 1.977 0.99
303_S 321_L 1.976 0.99
58_P 138_P 1.969 0.99
66_L 76_E 1.958 0.99
572_T 575_D 1.953 0.99
71_E 75_G 1.946 0.99
62_K 65_Q 1.936 0.99
145_T 150_K 1.935 0.99
200_E 204_A 1.919 0.99
389_K 449_A 1.902 0.99
584_A 612_V 1.902 0.99
587_E 609_K 1.902 0.99
109_L 113_R 1.896 0.99
584_A 633_M 1.894 0.99
501_E 505_A 1.88 0.99
130_D 179_S 1.863 0.98
113_R 117_N 1.845 0.98
79_Q 83_T 1.84 0.98
94_N 102_E 1.838 0.98
600_R 611_N 1.834 0.98
473_D 476_A 1.817 0.98
69_T 72_Q 1.81 0.98
465_A 474_L 1.799 0.98
32_A 48_F 1.797 0.98
119_F 197_S 1.747 0.97
502_R 505_A 1.747 0.97
146_C 149_C 1.725 0.97
68_I 73_M 1.704 0.97
456_V 481_G 1.702 0.97
207_R 224_E 1.696 0.97
126_S 185_M 1.69 0.97
603_L 612_V 1.686 0.96
442_V 495_V 1.684 0.96
602_T 609_K 1.682 0.96
589_A 614_S 1.682 0.96
38_Y 430_G 1.677 0.96
571_I 579_V 1.676 0.96
128_L 185_M 1.666 0.96
467_Q 470_R 1.665 0.96
83_T 87_G 1.661 0.96
596_D 618_S 1.66 0.96
416_P 420_K 1.657 0.96
203_Q 223_F 1.645 0.96
489_M 504_E 1.641 0.96
12_C 48_F 1.64 0.96
105_Q 109_L 1.64 0.96
75_G 97_S 1.619 0.95
194_D 197_S 1.611 0.95
54_A 97_S 1.605 0.95
148_K 164_A 1.602 0.95
62_K 66_L 1.601 0.95
613_F 645_G 1.599 0.95
115_K 245_N 1.589 0.95
331_G 372_D 1.588 0.95
7_K 45_E 1.585 0.95
629_R 665_S 1.583 0.94
114_L 312_G 1.581 0.94
379_V 537_M 1.579 0.94
605_L 633_M 1.578 0.94
40_R 46_V 1.574 0.94
422_F 448_L 1.573 0.94
121_K 194_D 1.559 0.94
459_Q 473_D 1.559 0.94
124_T 189_E 1.557 0.94
415_D 473_D 1.551 0.94
52_D 95_Y 1.541 0.93
94_N 100_S 1.54 0.93
145_T 173_E 1.515 0.93
498_K 502_R 1.507 0.92
328_T 372_D 1.506 0.92
272_R 276_V 1.487 0.92
78_S 82_Q 1.486 0.92
131_P 181_A 1.485 0.92
409_L 482_I 1.482 0.92
196_P 229_Q 1.476 0.91
362_A 381_R 1.474 0.91
579_V 634_V 1.466 0.91
68_I 72_Q 1.464 0.91
437_E 440_K 1.463 0.91
580_D 675_Q 1.453 0.90
130_D 146_C 1.445 0.90
599_L 623_P 1.444 0.90
612_V 651_V 1.439 0.90
429_I 445_I 1.434 0.90
603_L 633_M 1.429 0.89
133_K 179_S 1.427 0.89
194_D 229_Q 1.427 0.89
66_L 73_M 1.418 0.89
431_E 435_S 1.416 0.89
380_Q 384_A 1.416 0.89
583_V 632_I 1.416 0.89
195_L 308_A 1.408 0.89
421_T 451_L 1.405 0.88
130_D 149_C 1.401 0.88
614_S 653_A 1.4 0.88
36_V 91_S 1.394 0.88
457_D 460_A 1.38 0.87
629_R 663_L 1.375 0.87
121_K 229_Q 1.373 0.87
87_G 345_S 1.373 0.87
508_N 523_H 1.373 0.87
29_H 92_Y 1.373 0.87
425_A 448_L 1.372 0.87
538_R 541_E 1.37 0.87
429_I 433_W 1.369 0.87
585_L 604_D 1.368 0.87
605_L 608_E 1.363 0.86
105_Q 266_K 1.359 0.86
613_F 640_R 1.359 0.86
415_D 455_Y 1.356 0.86
83_T 86_A 1.355 0.86
573_F 577_A 1.333 0.85
101_E 104_R 1.327 0.84
586_I 589_A 1.326 0.84
433_W 495_V 1.323 0.84
601_L 651_V 1.318 0.84
593_E 596_D 1.317 0.84
614_S 651_V 1.315 0.83
630_H 669_G 1.313 0.83
585_L 672_P 1.311 0.83
445_I 492_L 1.31 0.83
581_L 634_V 1.31 0.83
586_I 631_T 1.309 0.83
593_E 600_R 1.309 0.83
87_G 349_N 1.308 0.83
630_H 672_P 1.304 0.83
589_A 599_L 1.301 0.83
366_S 437_E 1.29 0.82
424_D 428_V 1.29 0.82
94_N 267_N 1.289 0.82
38_Y 42_R 1.286 0.82
464_V 474_L 1.285 0.82
279_D 283_K 1.285 0.82
508_N 526_N 1.271 0.81
203_Q 224_E 1.267 0.80
493_K 504_E 1.266 0.80
656_P 660_D 1.265 0.80
7_K 280_E 1.26 0.80
582_R 635_A 1.258 0.80
311_E 317_K 1.246 0.79
12_C 32_A 1.242 0.78
54_A 99_H 1.242 0.78
11_T 14_L 1.238 0.78
571_I 634_V 1.233 0.78
451_L 458_E 1.23 0.77
632_I 666_P 1.223 0.77
60_M 64_Q 1.216 0.76
204_A 207_R 1.206 0.75
71_E 99_H 1.199 0.75
586_I 651_V 1.196 0.75
417_Q 421_T 1.194 0.74
28_E 324_H 1.194 0.74
191_F 246_A 1.19 0.74
631_T 651_V 1.188 0.74
201_M 314_N 1.185 0.74
492_L 496_L 1.184 0.74
574_D 577_A 1.181 0.73
654_A 670_A 1.173 0.73
120_I 232_I 1.171 0.72
592_V 645_G 1.171 0.72
601_L 614_S 1.17 0.72
589_A 653_A 1.169 0.72
153_D 338_R 1.168 0.72
579_V 584_A 1.165 0.72
410_A 522_G 1.155 0.71
611_N 645_G 1.154 0.71
129_Y 134_G 1.152 0.71
583_V 676_V 1.151 0.71
592_V 595_S 1.149 0.70
347_W 356_L 1.149 0.70
103_N 263_G 1.148 0.70
421_T 425_A 1.145 0.70
402_N 527_P 1.144 0.70
413_L 416_P 1.143 0.70
397_N 481_G 1.139 0.69
20_S 80_E 1.136 0.69
570_T 638_A 1.134 0.69
195_L 305_F 1.131 0.69
575_D 579_V 1.131 0.69
133_K 176_S 1.13 0.69
608_E 648_E 1.128 0.68
220_Q 224_E 1.127 0.68
594_G 643_R 1.124 0.68
533_N 539_Q 1.121 0.68
632_I 664_L 1.118 0.67
58_P 74_I 1.117 0.67
633_M 653_A 1.116 0.67
152_P 173_E 1.114 0.67
417_Q 420_K 1.114 0.67
441_A 495_V 1.107 0.66
401_I 525_V 1.102 0.66
145_T 152_P 1.101 0.66
583_V 630_H 1.1 0.65
275_S 279_D 1.099 0.65
590_E 594_G 1.098 0.65
410_A 475_Q 1.094 0.65
427_E 431_E 1.091 0.65
343_K 346_T 1.09 0.64
135_M 146_C 1.089 0.64
426_A 491_Y 1.087 0.64
37_R 40_R 1.086 0.64
113_R 116_E 1.085 0.64
539_Q 542_A 1.082 0.64
612_V 653_A 1.082 0.64
66_L 77_M 1.081 0.64
589_A 651_V 1.079 0.63
235_D 249_K 1.076 0.63
633_M 651_V 1.072 0.63
104_R 242_E 1.07 0.62
17_A 21_I 1.069 0.62
538_R 542_A 1.068 0.62
591_F 623_P 1.068 0.62
653_A 675_Q 1.068 0.62
632_I 663_L 1.065 0.62
587_E 602_T 1.063 0.62
419_Y 480_M 1.061 0.61
71_E 74_I 1.061 0.61
505_A 529_K 1.058 0.61
594_G 624_Q 1.057 0.61
19_G 25_H 1.055 0.61
653_A 677_K 1.055 0.61
473_D 477_I 1.047 0.60
125_I 192_F 1.046 0.60
13_A 17_A 1.045 0.60
586_I 666_P 1.045 0.60
398_A 528_F 1.042 0.59
656_P 671_K 1.041 0.59
662_F 668_A 1.038 0.59
42_R 430_G 1.038 0.59
449_A 488_L 1.032 0.58
454_R 457_D 1.03 0.58
13_A 28_E 1.03 0.58
472_A 476_A 1.027 0.58
651_V 663_L 1.025 0.57
580_D 635_A 1.022 0.57
632_I 676_V 1.021 0.57
572_T 638_A 1.021 0.57
512_T 515_G 1.02 0.57
73_M 77_M 1.018 0.57
586_I 634_V 1.018 0.57
560_G 563_A 1.017 0.57
415_D 476_A 1.014 0.56
94_N 271_K 1.011 0.56
537_M 541_E 1.01 0.56
445_I 491_Y 1.01 0.56
592_V 643_R 1.008 0.56
601_L 626_L 1.003 0.55
605_L 609_K 1.002 0.55
588_N 600_R 1.001 0.55
599_L 617_R 1 0.55
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1rqgA10.98381000.261Contact Map0.721
3h99A10.8081000.479Contact Map0.722
3kflA10.76811000.491Contact Map0.684
2x1lA30.74591000.54Contact Map0.746
4dlpA30.74591000.543Contact Map0.722
4lneA30.74591000.543Contact Map0.715
2csxA20.71941000.546Contact Map0.807
2d5bA10.72081000.555Contact Map0.711
3u1fA20.75781000.556Contact Map0.718
1ileA10.78731000.621Contact Map0.59
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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