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OPENSEQ.org
SYA - Alanine--tRNA ligase
UniProt: P00957 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10034
Length: 876 (864)
Sequences: 1973
Seq/Len: 2.28
SYA
Paralog alert: 0.02 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
483_I 489_A 5.75 1.00
114_L 121_F 5.04 1.00
14_D 17_H 4.4 1.00
464_L 552_V 4.272 1.00
10_Q 14_D 4.071 1.00
766_I 868_K 4.027 1.00
604_M 612_V 3.943 1.00
468_G 494_D 3.889 1.00
519_F 535_G 3.881 1.00
563_N 699_T 3.799 1.00
623_N 673_R 3.677 1.00
827_K 830_E 3.628 1.00
565_S 658_M 3.623 1.00
280_S 320_H 3.492 1.00
632_D 635_A 3.472 1.00
493_L 550_A 3.467 1.00
105_H 146_E 3.457 1.00
777_E 861_P 3.321 1.00
141_E 145_K 3.303 1.00
266_A 269_K 3.286 1.00
604_M 609_I 3.255 1.00
507_G 524_T 3.153 1.00
556_R 560_I 3.105 1.00
10_Q 23_V 2.945 1.00
294_L 299_V 2.858 1.00
395_A 420_V 2.808 1.00
395_A 406_V 2.803 1.00
799_S 821_D 2.751 1.00
428_A 431_E 2.685 1.00
859_A 862_A 2.673 1.00
19_K 113_E 2.655 1.00
475_V 487_Q 2.63 1.00
421_D 424_G 2.624 1.00
576_Q 615_L 2.603 1.00
520_A 538_A 2.545 1.00
767_D 770_G 2.531 1.00
611_A 614_D 2.53 1.00
430_E 434_R 2.527 1.00
467_N 547_A 2.515 1.00
619_Q 622_R 2.514 1.00
410_A 422_E 2.492 1.00
336_G 357_E 2.477 1.00
739_T 742_L 2.466 1.00
623_N 675_G 2.445 1.00
856_D 859_A 2.437 1.00
457_E 495_Q 2.428 1.00
805_A 860_L 2.412 1.00
459_K 466_L 2.395 1.00
790_V 817_G 2.391 1.00
144_E 151_R 2.39 1.00
767_D 772_K 2.343 1.00
11_A 114_L 2.315 1.00
577_V 615_L 2.303 1.00
717_V 732_V 2.293 1.00
473_L 491_V 2.277 1.00
88_H 254_N 2.27 1.00
465_E 551_D 2.256 1.00
13_L 23_V 2.248 1.00
261_R 265_Q 2.241 1.00
378_L 412_V 2.238 1.00
222_Q 226_T 2.223 1.00
302_S 307_G 2.211 1.00
608_E 611_A 2.205 1.00
746_L 749_L 2.186 1.00
602_E 608_E 2.186 1.00
389_T 419_K 2.183 1.00
671_A 677_I 2.166 1.00
27_S 46_Q 2.149 1.00
616_V 680_F 2.142 1.00
222_Q 228_E 2.13 1.00
509_K 556_R 2.124 1.00
221_R 227_M 2.12 1.00
581_H 600_H 2.103 1.00
803_V 816_A 2.086 0.99
512_L 548_V 2.08 0.99
391_D 394_T 2.076 0.99
469_K 547_A 2.075 0.99
130_V 149_I 2.063 0.99
610_R 614_D 2.06 0.99
633_L 637_K 2.059 0.99
717_V 728_L 2.03 0.99
141_E 144_E 2.02 0.99
343_G 350_K 2 0.99
194_W 202_E 1.996 0.99
17_H 23_V 1.988 0.99
745_E 750_K 1.981 0.99
488_E 536_K 1.976 0.99
630_I 652_R 1.962 0.99
430_E 433_R 1.962 0.99
15_F 19_K 1.962 0.99
390_L 413_C 1.961 0.99
121_F 248_L 1.958 0.99
836_A 852_A 1.956 0.99
328_E 333_K 1.955 0.99
137_D 156_R 1.947 0.99
866_S 869_G 1.944 0.99
105_H 145_K 1.942 0.99
635_A 638_A 1.931 0.99
782_E 785_M 1.924 0.99
332_Y 361_K 1.88 0.99
562_L 621_R 1.87 0.99
113_E 117_S 1.863 0.99
422_E 426_E 1.859 0.99
115_L 211_I 1.859 0.99
366_Q 369_R 1.859 0.99
605_K 608_E 1.856 0.99
600_H 608_E 1.83 0.98
491_V 512_L 1.822 0.98
128_L 185_I 1.811 0.98
431_E 435_R 1.793 0.98
764_K 776_S 1.786 0.98
381_E 390_L 1.784 0.98
520_A 536_K 1.779 0.98
489_A 519_F 1.774 0.98
493_L 512_L 1.773 0.98
572_A 576_Q 1.772 0.98
388_D 419_K 1.772 0.98
406_V 422_E 1.77 0.98
268_A 281_L 1.765 0.98
144_E 154_I 1.757 0.98
14_D 23_V 1.754 0.98
13_L 63_T 1.74 0.98
832_I 852_A 1.729 0.97
861_P 865_A 1.722 0.97
838_Q 859_A 1.716 0.97
267_V 281_L 1.709 0.97
321_G 330_F 1.701 0.97
314_I 360_L 1.697 0.97
742_L 746_L 1.686 0.97
620_I 680_F 1.685 0.97
42_A 45_N 1.683 0.97
106_D 110_F 1.673 0.97
737_E 741_Q 1.672 0.97
117_S 125_K 1.662 0.96
199_G 203_E 1.657 0.96
129_W 155_I 1.652 0.96
818_V 822_V 1.639 0.96
542_L 548_V 1.636 0.96
291_C 314_I 1.634 0.96
211_I 247_V 1.614 0.96
519_F 542_L 1.602 0.95
482_A 543_K 1.596 0.95
15_F 113_E 1.589 0.95
400_D 432_Q 1.588 0.95
114_L 248_L 1.584 0.95
470_V 542_L 1.584 0.95
209_I 246_A 1.583 0.95
339_I 353_Q 1.583 0.95
353_Q 357_E 1.579 0.95
361_K 365_E 1.578 0.95
381_E 394_T 1.57 0.95
111_A 214_I 1.568 0.95
265_Q 268_A 1.566 0.95
109_Q 146_E 1.562 0.95
467_N 549_Q 1.554 0.94
423_A 427_A 1.545 0.94
760_N 763_S 1.544 0.94
123_L 247_V 1.538 0.94
199_G 205_G 1.537 0.94
468_G 495_Q 1.535 0.94
456_S 531_I 1.533 0.94
296_A 356_V 1.531 0.94
434_R 438_E 1.529 0.94
469_K 494_D 1.527 0.94
18_S 118_E 1.513 0.93
140_Y 144_E 1.496 0.93
626_I 671_A 1.495 0.93
491_V 542_L 1.489 0.93
559_R 700_G 1.486 0.92
124_P 127_R 1.486 0.92
50_V 57_R 1.479 0.92
271_T 326_A 1.477 0.92
512_L 550_A 1.471 0.92
575_R 583_S 1.449 0.91
626_I 658_M 1.449 0.91
479_A 544_V 1.446 0.91
61_R 101_D 1.446 0.91
482_A 541_S 1.441 0.91
634_E 638_A 1.436 0.91
134_E 159_D 1.426 0.90
15_F 110_F 1.419 0.90
518_S 539_A 1.415 0.90
358_Q 602_E 1.415 0.90
8_I 245_A 1.415 0.90
229_P 233_P 1.41 0.90
183_T 214_I 1.409 0.90
105_H 142_I 1.4 0.89
23_V 63_T 1.393 0.89
464_L 554_E 1.39 0.89
378_L 382_L 1.385 0.88
262_T 341_V 1.382 0.88
834_M 866_S 1.381 0.88
465_E 549_Q 1.376 0.88
106_D 109_Q 1.372 0.88
65_S 346_G 1.371 0.88
336_G 353_Q 1.369 0.88
790_V 815_I 1.367 0.88
189_H 209_I 1.365 0.87
118_E 122_A 1.36 0.87
720_L 733_R 1.359 0.87
10_Q 17_H 1.358 0.87
406_V 420_V 1.356 0.87
797_L 800_T 1.35 0.87
489_A 535_G 1.347 0.86
266_A 337_P 1.345 0.86
837_Q 842_K 1.345 0.86
556_R 591_D 1.344 0.86
514_G 517_F 1.342 0.86
682_I 695_I 1.34 0.86
672_S 676_D 1.338 0.86
639_K 663_T 1.338 0.86
701_E 705_A 1.337 0.86
40_T 45_N 1.335 0.86
607_E 611_A 1.335 0.86
812_V 857_A 1.334 0.86
132_V 156_R 1.334 0.86
790_V 802_I 1.334 0.86
423_A 426_E 1.334 0.86
268_A 271_T 1.331 0.86
83_Y 414_R 1.329 0.85
105_H 109_Q 1.328 0.85
575_R 642_M 1.318 0.85
264_I 268_A 1.317 0.85
631_M 639_K 1.316 0.85
429_M 432_Q 1.315 0.85
543_K 546_D 1.314 0.85
657_S 663_T 1.314 0.85
590_N 593_V 1.313 0.85
459_K 495_Q 1.297 0.84
137_D 141_E 1.297 0.84
427_A 431_E 1.293 0.83
500_A 531_I 1.291 0.83
812_V 839_V 1.29 0.83
830_E 833_G 1.288 0.83
140_Y 156_R 1.288 0.83
513_K 551_D 1.281 0.83
803_V 867_V 1.276 0.82
632_D 652_R 1.275 0.82
838_Q 866_S 1.269 0.82
521_V 535_G 1.268 0.82
483_I 519_F 1.267 0.82
470_V 491_V 1.265 0.81
41_N 173_M 1.261 0.81
502_S 526_K 1.256 0.81
335_V 339_I 1.255 0.81
571_H 642_M 1.249 0.80
251_V 256_D 1.248 0.80
614_D 618_T 1.245 0.80
807_V 860_L 1.242 0.80
189_H 246_A 1.242 0.80
840_G 856_D 1.242 0.80
11_A 121_F 1.241 0.80
24_V 64_T 1.237 0.79
463_H 552_V 1.236 0.79
629_N 639_K 1.236 0.79
142_I 146_E 1.234 0.79
625_P 673_R 1.234 0.79
314_I 318_V 1.231 0.79
266_A 270_V 1.231 0.79
569_L 616_V 1.229 0.79
484_N 487_Q 1.228 0.79
10_Q 344_S 1.221 0.78
702_G 705_A 1.221 0.78
563_N 702_G 1.22 0.78
17_H 22_Q 1.218 0.78
560_I 591_D 1.218 0.78
37_L 41_N 1.217 0.78
610_R 712_D 1.215 0.78
634_E 637_K 1.207 0.77
142_I 147_V 1.203 0.77
623_N 676_D 1.202 0.77
129_W 208_Y 1.201 0.77
288_I 334_L 1.197 0.76
485_A 539_A 1.189 0.76
257_I 262_T 1.189 0.76
292_A 335_V 1.188 0.75
124_P 187_Y 1.187 0.75
585_K 599_S 1.186 0.75
129_W 153_R 1.184 0.75
713_R 729_A 1.179 0.75
842_K 852_A 1.172 0.74
354_A 358_Q 1.169 0.74
189_H 252_N 1.169 0.74
22_Q 62_A 1.166 0.73
784_K 842_K 1.166 0.73
127_R 187_Y 1.165 0.73
382_L 418_I 1.163 0.73
506_V 560_I 1.163 0.73
738_R 743_E 1.16 0.73
263_L 338_L 1.156 0.73
807_V 810_G 1.155 0.72
507_G 523_D 1.153 0.72
679_L 707_V 1.153 0.72
407_D 411_D 1.153 0.72
839_V 859_A 1.149 0.72
426_E 430_E 1.149 0.72
24_V 62_A 1.148 0.72
522_E 535_G 1.146 0.72
488_E 522_E 1.146 0.72
431_E 434_R 1.145 0.72
350_K 353_Q 1.145 0.72
183_T 235_V 1.143 0.71
792_D 795_N 1.142 0.71
842_K 855_T 1.141 0.71
40_T 46_Q 1.14 0.71
820_K 824_D 1.14 0.71
220_N 228_E 1.139 0.71
14_D 18_S 1.136 0.71
6_A 344_S 1.135 0.71
774_L 793_L 1.132 0.70
151_R 154_I 1.132 0.70
570_M 594_L 1.128 0.70
628_T 656_L 1.128 0.70
133_Y 159_D 1.126 0.70
594_L 697_A 1.125 0.70
140_Y 151_R 1.125 0.70
5_T 259_L 1.124 0.70
19_K 110_F 1.122 0.69
727_N 730_D 1.121 0.69
266_A 333_K 1.118 0.69
80_N 84_T 1.118 0.69
614_D 708_H 1.118 0.69
714_L 717_V 1.116 0.69
865_A 869_G 1.115 0.69
518_S 538_A 1.111 0.68
658_M 674_T 1.111 0.68
765_A 774_L 1.109 0.68
493_L 548_V 1.108 0.68
22_Q 59_Y 1.108 0.68
803_V 835_V 1.106 0.68
773_L 871_V 1.103 0.67
525_Q 534_I 1.101 0.67
174_G 234_S 1.101 0.67
29_L 69_V 1.101 0.67
814_L 839_V 1.1 0.67
64_T 97_F 1.1 0.67
78_L 313_I 1.097 0.67
483_I 487_Q 1.097 0.67
11_A 118_E 1.092 0.66
413_C 418_I 1.091 0.66
470_V 512_L 1.088 0.66
71_A 78_L 1.088 0.66
522_E 536_K 1.088 0.66
29_L 294_L 1.087 0.66
470_V 493_L 1.087 0.66
537_L 542_L 1.087 0.66
558_A 561_R 1.086 0.66
642_M 665_L 1.085 0.66
805_A 864_L 1.085 0.66
28_S 32_H 1.079 0.65
799_S 819_S 1.078 0.65
833_G 837_Q 1.075 0.65
109_Q 113_E 1.075 0.65
470_V 546_D 1.072 0.64
833_G 842_K 1.07 0.64
509_K 560_I 1.067 0.64
69_V 290_S 1.063 0.63
117_S 122_A 1.063 0.63
865_A 868_K 1.062 0.63
217_M 234_S 1.061 0.63
426_E 433_R 1.061 0.63
266_A 334_L 1.061 0.63
681_R 711_S 1.059 0.63
820_K 848_D 1.056 0.62
319_R 415_E 1.055 0.62
710_D 714_L 1.053 0.62
619_Q 624_L 1.053 0.62
868_K 872_S 1.049 0.62
615_L 661_F 1.046 0.61
862_A 865_A 1.044 0.61
211_I 244_I 1.044 0.61
838_Q 862_A 1.043 0.61
827_K 847_P 1.042 0.61
468_G 550_A 1.041 0.61
490_V 534_I 1.038 0.61
42_A 68_C 1.037 0.60
769_N 872_S 1.037 0.60
689_A 692_V 1.035 0.60
679_L 704_I 1.034 0.60
301_P 359_V 1.033 0.60
712_D 716_E 1.032 0.60
413_C 420_V 1.031 0.60
139_A 183_T 1.029 0.60
778_L 789_M 1.025 0.59
869_G 872_S 1.025 0.59
57_R 62_A 1.022 0.59
59_Y 62_A 1.021 0.59
470_V 548_V 1.02 0.59
7_E 11_A 1.019 0.58
115_L 185_I 1.018 0.58
497_P 550_A 1.016 0.58
519_F 537_L 1.015 0.58
322_N 327_K 1.014 0.58
748_Q 751_E 1.01 0.57
728_L 732_V 1.009 0.57
800_T 819_S 1.007 0.57
15_F 117_S 1.007 0.57
197_P 200_S 1.006 0.57
629_N 657_S 1.005 0.57
698_V 703_A 1.003 0.57
214_I 235_V 1.002 0.56
251_V 261_R 1.002 0.56
631_M 635_A 1.002 0.56
198_P 205_G 1.001 0.56
198_P 203_E 1 0.56
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2zzeA20.66321000.341Contact Map0.772
2ztgA10.69751000.349Contact Map0.713
3hy0A20.50341000.42Contact Map0.8
1yfsA20.50341000.427Contact Map0.802
3kewA20.2581000.823Contact Map0.667
2e1bA10.24091000.834Contact Map0.738
1nyrA20.40641000.909Contact Map0.641
1v4pA30.16211000.91Contact Map0.787
1tkeA10.23631000.911Contact Map0.688
2zvfA80.19411000.914Contact Map0.736
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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