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OPENSEQ.org
MPPA - Periplasmic murein peptide-binding protein
UniProt: P77348 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13376
Length: 537 (503)
Sequences: 9340
Seq/Len: 18.57
MPPA
Paralog alert: 0.87 [within 20: 0.29] - ratio of genomes with paralogs
Cluster includes: DDPA DPPA GSIB MPPA NIKA OPPA SAPA SGRR YBAE YEJA YGIS
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
215_K 226_V 4.657 1.00
308_R 370_A 4.023 1.00
320_R 324_E 3.972 1.00
162_D 165_T 3.891 1.00
161_V 167_K 3.86 1.00
363_Q 366_T 3.808 1.00
159_T 167_K 3.802 1.00
100_D 164_H 3.52 1.00
213_V 226_V 3.389 1.00
476_E 481_A 3.351 1.00
468_K 472_Q 3.338 1.00
461_F 496_Q 3.188 1.00
116_V 120_Q 3.156 1.00
116_V 160_A 3.107 1.00
85_Q 97_T 3.037 1.00
380_K 409_D 2.969 1.00
113_Q 160_A 2.914 1.00
318_D 321_L 2.893 1.00
366_T 370_A 2.89 1.00
313_L 402_W 2.879 1.00
465_A 468_K 2.86 1.00
315_M 363_Q 2.821 1.00
87_K 97_T 2.797 1.00
302_G 305_A 2.795 1.00
73_N 77_E 2.729 1.00
307_Q 311_L 2.585 1.00
380_K 411_K 2.581 1.00
70_V 86_W 2.564 1.00
481_A 484_A 2.554 1.00
308_R 367_L 2.534 1.00
308_R 311_L 2.514 1.00
463_N 496_Q 2.513 1.00
110_V 209_N 2.479 1.00
463_N 492_I 2.477 1.00
93_I 167_K 2.39 1.00
159_T 169_Q 2.359 1.00
93_I 169_Q 2.335 1.00
88_S 92_R 2.334 1.00
95_T 167_K 2.321 1.00
403_K 409_D 2.319 1.00
119_W 168_I 2.313 1.00
478_T 481_A 2.312 1.00
191_K 195_E 2.31 1.00
97_T 165_T 2.295 1.00
465_A 488_A 2.293 1.00
213_V 229_T 2.286 1.00
84_T 100_D 2.282 1.00
73_N 79_V 2.247 1.00
102_A 110_V 2.245 1.00
103_K 109_P 2.221 1.00
105_A 228_N 2.215 1.00
103_K 230_H 2.205 1.00
115_F 166_L 2.2 1.00
472_Q 485_D 2.194 1.00
224_V 241_K 2.142 1.00
452_S 467_D 2.137 1.00
382_T 411_K 2.124 1.00
484_A 488_A 2.113 1.00
382_T 413_Q 2.093 1.00
212_Y 239_L 2.062 1.00
452_S 462_N 2.044 1.00
311_L 363_Q 2.038 1.00
297_F 500_A 2.029 1.00
108_T 192_A 2.02 1.00
111_T 114_D 2.014 1.00
301_K 430_D 2.003 1.00
49_D 121_R 1.995 1.00
67_E 81_G 1.987 1.00
101_N 109_P 1.973 1.00
466_Y 489_A 1.951 1.00
36_E 241_K 1.894 1.00
491_K 495_E 1.891 1.00
469_V 488_A 1.883 1.00
113_Q 191_K 1.881 1.00
113_Q 163_A 1.868 1.00
256_R 261_D 1.847 1.00
473_A 486_Y 1.844 1.00
218_V 221_E 1.844 1.00
313_L 408_V 1.843 1.00
216_E 224_V 1.839 1.00
297_F 431_V 1.838 1.00
113_Q 116_V 1.833 1.00
84_T 97_T 1.812 1.00
334_A 504_Q 1.8 1.00
473_A 485_D 1.766 1.00
485_D 488_A 1.759 1.00
36_E 243_T 1.755 1.00
314_S 497_A 1.75 1.00
86_W 96_F 1.749 1.00
226_V 241_K 1.74 1.00
488_A 491_K 1.728 1.00
317_I 398_V 1.726 1.00
40_H 262_I 1.719 1.00
399_A 403_K 1.712 1.00
114_D 191_K 1.709 1.00
227_P 240_Q 1.7 1.00
216_E 222_K 1.695 1.00
55_G 58_E 1.681 1.00
106_D 190_Q 1.677 1.00
307_Q 496_Q 1.677 1.00
304_T 313_L 1.672 1.00
258_L 275_K 1.669 1.00
70_V 96_F 1.658 1.00
112_A 160_A 1.654 1.00
304_T 381_L 1.653 1.00
472_Q 481_A 1.622 1.00
365_K 369_S 1.619 1.00
464_P 468_K 1.613 1.00
215_K 241_K 1.609 1.00
102_A 209_N 1.603 1.00
108_T 190_Q 1.598 1.00
465_A 492_I 1.598 1.00
469_V 485_D 1.584 1.00
218_V 222_K 1.58 1.00
316_T 406_L 1.569 1.00
111_T 163_A 1.567 1.00
309_V 371_A 1.559 1.00
309_V 368_L 1.548 1.00
362_A 365_K 1.543 1.00
281_P 513_P 1.539 1.00
84_T 99_R 1.538 1.00
369_S 374_G 1.529 1.00
423_S 428_N 1.526 1.00
105_A 213_V 1.525 1.00
247_I 256_R 1.522 1.00
95_T 165_T 1.52 1.00
484_A 487_N 1.515 1.00
422_D 426_T 1.505 1.00
38_V 243_T 1.504 1.00
190_Q 193_N 1.494 1.00
222_K 243_T 1.492 1.00
462_N 467_D 1.491 1.00
367_L 370_A 1.49 1.00
300_Q 460_R 1.483 1.00
481_A 485_D 1.476 1.00
109_P 164_H 1.475 1.00
168_I 177_F 1.469 1.00
161_V 165_T 1.456 1.00
416_E 419_T 1.455 1.00
88_S 91_N 1.45 1.00
274_Q 277_L 1.443 1.00
99_R 102_A 1.426 1.00
67_E 209_N 1.423 1.00
311_L 497_A 1.422 1.00
483_N 487_N 1.421 1.00
97_T 164_H 1.402 0.99
442_E 482_R 1.394 0.99
307_Q 495_E 1.381 0.99
492_I 496_Q 1.372 0.99
35_Q 240_Q 1.371 0.99
224_V 243_T 1.366 0.99
94_W 177_F 1.366 0.99
88_S 93_I 1.36 0.99
476_E 485_D 1.359 0.99
256_R 262_I 1.35 0.99
67_E 232_W 1.346 0.99
378_P 409_D 1.344 0.99
469_V 492_I 1.334 0.99
112_A 161_V 1.332 0.99
157_G 169_Q 1.329 0.99
392_Q 396_I 1.322 0.99
103_K 228_N 1.316 0.99
360_L 363_Q 1.316 0.99
320_R 333_P 1.315 0.99
88_S 95_T 1.31 0.99
323_T 333_P 1.308 0.99
366_T 369_S 1.3 0.99
283_Q 513_P 1.295 0.99
263_D 512_K 1.294 0.99
225_V 244_F 1.294 0.99
160_A 163_A 1.293 0.99
227_P 237_T 1.284 0.99
252_A 256_R 1.269 0.99
116_V 158_V 1.255 0.99
105_A 214_L 1.254 0.99
105_A 230_H 1.238 0.98
226_V 229_T 1.236 0.98
97_T 100_D 1.236 0.98
461_F 466_Y 1.226 0.98
301_K 305_A 1.224 0.98
401_M 404_K 1.223 0.98
413_Q 423_S 1.214 0.98
82_V 209_N 1.214 0.98
427_G 460_R 1.213 0.98
233_D 236_K 1.201 0.98
362_A 366_T 1.2 0.98
263_D 514_W 1.187 0.98
465_A 469_V 1.18 0.98
311_L 367_L 1.173 0.98
383_L 410_V 1.17 0.97
84_T 164_H 1.169 0.97
215_K 224_V 1.169 0.97
80_P 86_W 1.167 0.97
44_E 217_R 1.164 0.97
158_V 166_L 1.164 0.97
97_T 162_D 1.158 0.97
361_N 406_L 1.154 0.97
172_K 475_T 1.151 0.97
469_V 472_Q 1.151 0.97
106_D 192_A 1.147 0.97
115_F 188_P 1.145 0.97
468_K 471_A 1.144 0.97
96_F 168_I 1.14 0.97
192_A 196_S 1.126 0.97
397_A 401_M 1.124 0.97
297_F 313_L 1.122 0.97
472_Q 488_A 1.121 0.97
361_N 365_K 1.109 0.96
40_H 253_A 1.106 0.96
109_P 163_A 1.104 0.96
381_L 431_V 1.104 0.96
399_A 412_L 1.103 0.96
402_W 410_V 1.102 0.96
307_Q 497_A 1.101 0.96
71_N 79_V 1.1 0.96
38_V 245_L 1.099 0.96
385_Y 412_L 1.099 0.96
87_K 95_T 1.094 0.96
472_Q 476_E 1.093 0.96
99_R 232_W 1.091 0.96
141_N 169_Q 1.089 0.96
381_L 410_V 1.085 0.96
258_L 279_D 1.085 0.96
257_Y 510_L 1.08 0.95
68_G 81_G 1.08 0.95
321_L 401_M 1.079 0.95
71_N 81_G 1.079 0.95
340_D 504_Q 1.078 0.95
227_P 236_K 1.077 0.95
141_N 171_D 1.074 0.95
95_T 161_V 1.073 0.95
398_V 402_W 1.07 0.95
94_W 178_V 1.07 0.95
400_S 404_K 1.069 0.95
417_W 421_I 1.068 0.95
516_K 535_V 1.064 0.95
322_M 394_I 1.047 0.94
461_F 498_P 1.044 0.94
480_K 483_N 1.04 0.94
316_T 402_W 1.036 0.94
286_T 506_T 1.034 0.94
114_D 117_Y 1.029 0.94
120_Q 160_A 1.019 0.93
213_V 228_N 1.015 0.93
160_A 167_K 1.015 0.93
283_Q 511_I 1.013 0.93
296_A 432_I 1.011 0.93
327_L 331_E 1.01 0.93
308_R 371_A 1.009 0.93
116_V 166_L 1.005 0.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3o9pA10.96461000.112Contact Map0.744
1jetA10.95721000.122Contact Map0.807
3zs6A10.93671000.125Contact Map0.765
4fajA10.97391000.127Contact Map0.733
4gl8A20.97021000.149Contact Map0.805
1uqwA20.91431000.17Contact Map0.781
1xocA10.91431000.17Contact Map0.755
3m8uA10.90881000.172Contact Map0.795
1dpeA10.90131000.175Contact Map0.797
3tpaA10.91811000.178Contact Map0.758
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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