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OPENSEQ.org
THIM - Hydroxyethylthiazole kinase
UniProt: P76423 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG14069
Length: 262 (250)
Sequences: 866
Seq/Len: 3.46
THIM
Paralog alert: 0.08 [within 20: 0.05] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
193_M 232_G 3.163 1.00
123_A 211_C 2.975 1.00
33_V 198_G 2.869 1.00
46_A 209_A 2.69 1.00
188_G 229_K 2.595 1.00
49_A 60_F 2.575 1.00
68_L 208_A 2.553 1.00
242_P 254_W 2.549 1.00
123_A 207_V 2.549 1.00
33_V 37_T 2.543 1.00
40_T 202_A 2.532 1.00
257_T 260_V 2.423 1.00
184_I 218_L 2.342 1.00
125_R 204_S 2.293 1.00
171_T 225_C 2.266 1.00
47_S 145_D 2.239 1.00
83_R 115_E 2.118 1.00
86_V 119_F 2.104 1.00
27_C 41_L 2.096 1.00
40_T 253_L 2.013 1.00
122_A 217_T 2.01 1.00
88_Q 92_S 2.007 1.00
188_G 230_Q 1.974 1.00
41_L 205_A 1.968 1.00
79_A 112_F 1.914 1.00
59_Q 107_D 1.895 1.00
52_I 78_R 1.831 0.99
129_S 141_G 1.83 0.99
49_A 102_A 1.829 0.99
196_V 199_T 1.819 0.99
161_A 179_D 1.806 0.99
116_L 121_P 1.795 0.99
31_D 34_Q 1.788 0.99
123_A 221_V 1.786 0.99
157_A 170_V 1.786 0.99
188_G 233_E 1.776 0.99
71_V 113_C 1.764 0.99
27_C 205_A 1.726 0.99
190_D 236_V 1.638 0.98
161_A 180_G 1.631 0.98
234_R 252_A 1.63 0.98
182_R 216_D 1.627 0.98
41_L 209_A 1.623 0.98
169_V 221_V 1.607 0.98
34_Q 52_I 1.605 0.98
68_L 204_S 1.59 0.98
161_A 178_T 1.569 0.98
37_T 205_A 1.568 0.98
210_C 227_W 1.516 0.97
36_F 246_V 1.508 0.97
179_D 182_R 1.507 0.97
126_G 131_I 1.497 0.97
36_F 202_A 1.482 0.96
184_I 222_A 1.478 0.96
34_Q 78_R 1.467 0.96
177_V 186_I 1.429 0.95
176_Y 185_G 1.399 0.95
52_I 75_T 1.396 0.95
44_L 206_V 1.387 0.94
60_F 102_A 1.375 0.94
107_D 111_H 1.347 0.93
55_E 76_Q 1.344 0.93
121_P 124_I 1.332 0.93
96_W 121_P 1.329 0.92
25_V 212_A 1.328 0.92
213_L 220_N 1.317 0.92
207_V 224_A 1.297 0.91
174_M 187_H 1.29 0.91
28_M 67_L 1.284 0.91
186_I 222_A 1.282 0.91
96_W 119_F 1.279 0.90
60_F 106_L 1.272 0.90
157_A 178_T 1.27 0.90
90_K 121_P 1.26 0.89
243_G 250_L 1.251 0.89
27_C 68_L 1.249 0.89
248_H 251_D 1.247 0.89
174_M 185_G 1.245 0.89
199_T 232_G 1.223 0.88
132_M 153_A 1.211 0.87
216_D 219_E 1.185 0.85
35_T 197_V 1.184 0.85
18_F 213_L 1.182 0.85
206_V 228_M 1.18 0.85
115_E 118_S 1.156 0.83
234_R 260_V 1.144 0.82
21_H 65_S 1.141 0.82
228_M 232_G 1.136 0.82
129_S 132_M 1.128 0.81
228_M 249_F 1.116 0.80
51_V 60_F 1.099 0.79
244_S 248_H 1.098 0.79
252_A 255_Q 1.095 0.78
244_S 247_P 1.081 0.77
206_V 249_F 1.08 0.77
194_T 232_G 1.075 0.77
42_L 197_V 1.071 0.76
244_S 251_D 1.057 0.75
203_L 225_C 1.055 0.75
204_S 208_A 1.049 0.74
75_T 78_R 1.047 0.74
167_I 221_V 1.038 0.73
131_I 168_V 1.034 0.73
31_D 52_I 1.03 0.72
61_A 88_Q 1.026 0.72
33_V 36_F 1.025 0.72
79_A 108_Y 1.015 0.71
41_L 125_R 1.012 0.70
31_D 78_R 1.01 0.70
35_T 39_N 1.009 0.70
58_S 88_Q 1.003 0.69
246_V 250_L 1.002 0.69
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3dzvA20.97711000.409Contact Map0.708
1ekqA20.98091000.436Contact Map0.726
3nl6A30.99241000.544Contact Map0.786
1v8aA10.96951000.578Contact Map0.785
3bgkA10.919899.90.732Contact Map0.393
2r3bA20.912299.90.735Contact Map0.473
3k5wA10.881799.90.754Contact Map0.487
3rpzA10.900899.90.766Contact Map0.515
3rssA10.904699.90.771Contact Map0.556
2yxtA20.893199.70.805Contact Map0.462
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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