May 4, 2021 - We are working on upgrading the webserver, some pages may not work.
OPENSEQ.org
RLMF - Ribosomal RNA large subunit methyltransferase F
UniProt: P75782 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13319
Length: 308 (302)
Sequences: 407
Seq/Len: 1.35
RLMF
Paralog alert: 0.01 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
107_I 181_A 4.853 1.00
122_G 130_F 3.672 1.00
233_I 254_V 3.184 1.00
140_L 159_L 3.143 1.00
263_L 290_I 3.013 1.00
264_Y 277_K 2.724 1.00
285_K 288_R 2.697 1.00
278_K 289_F 2.482 1.00
117_I 121_I 2.239 0.99
131_T 179_Y 2.209 0.99
112_V 116_C 2.075 0.98
112_V 132_G 2.054 0.98
134_E 140_L 2.047 0.98
248_M 303_F 1.942 0.97
13_R 125_E 1.939 0.97
182_T 236_M 1.88 0.96
90_L 181_A 1.877 0.96
9_H 12_N 1.869 0.96
169_F 236_M 1.862 0.96
237_I 260_L 1.861 0.96
116_C 143_A 1.804 0.95
119_P 130_F 1.67 0.91
45_D 48_N 1.668 0.91
274_K 293_T 1.637 0.90
53_K 70_D 1.613 0.89
61_A 67_A 1.56 0.87
120_L 154_N 1.542 0.86
107_I 183_L 1.53 0.86
90_L 107_I 1.528 0.85
75_F 136_S 1.515 0.85
11_R 92_D 1.487 0.83
280_M 289_F 1.476 0.83
254_V 263_L 1.471 0.82
116_C 147_I 1.447 0.81
122_G 128_W 1.442 0.81
190_D 194_A 1.401 0.78
107_I 130_F 1.385 0.77
216_F 223_L 1.383 0.77
24_C 30_L 1.379 0.76
45_D 233_I 1.375 0.76
29_E 57_K 1.371 0.76
106_S 131_T 1.352 0.74
230_V 259_N 1.346 0.74
131_T 158_R 1.342 0.74
19_D 22_T 1.338 0.73
59_L 71_I 1.334 0.73
260_L 290_I 1.328 0.72
28_P 260_L 1.32 0.72
142_S 290_I 1.317 0.72
27_N 54_A 1.317 0.72
123_V 156_A 1.31 0.71
21_A 24_C 1.298 0.70
160_R 177_E 1.292 0.69
64_Y 84_A 1.267 0.67
181_A 251_T 1.265 0.67
182_T 247_V 1.262 0.67
84_A 121_I 1.261 0.67
17_R 41_E 1.248 0.66
112_V 133_S 1.239 0.65
167_A 239_E 1.236 0.64
77_C 202_K 1.236 0.64
24_C 34_L 1.233 0.64
266_A 270_V 1.233 0.64
120_L 152_G 1.231 0.64
71_I 282_Q 1.224 0.63
30_L 58_A 1.22 0.63
79_P 288_R 1.219 0.63
20_L 34_L 1.218 0.63
85_D 282_Q 1.21 0.62
14_H 18_Y 1.209 0.62
33_F 51_A 1.205 0.62
216_F 224_W 1.199 0.61
280_M 288_R 1.198 0.61
87_I 165_S 1.196 0.61
61_A 190_D 1.195 0.61
300_R 303_F 1.191 0.60
29_E 54_A 1.184 0.60
177_E 193_A 1.181 0.59
234_K 266_A 1.18 0.59
116_C 157_I 1.179 0.59
144_Q 147_I 1.173 0.58
108_L 131_T 1.17 0.58
115_N 132_G 1.168 0.58
189_H 214_L 1.162 0.57
26_V 62_H 1.162 0.57
56_N 215_N 1.156 0.57
299_Q 302_R 1.155 0.57
90_L 207_G 1.153 0.57
237_I 290_I 1.15 0.56
157_I 237_I 1.145 0.56
91_A 126_Y 1.144 0.56
87_I 93_L 1.144 0.56
90_L 289_F 1.136 0.55
164_E 167_A 1.135 0.55
10_P 139_A 1.13 0.54
88_H 92_D 1.127 0.54
233_I 252_S 1.121 0.53
280_M 285_K 1.116 0.53
115_N 225_C 1.111 0.52
84_A 91_A 1.109 0.52
20_L 24_C 1.096 0.51
66_V 203_R 1.096 0.51
296_N 299_Q 1.094 0.51
261_P 266_A 1.093 0.51
206_L 255_S 1.092 0.50
145_A 149_S 1.092 0.50
132_G 265_R 1.091 0.50
132_G 177_E 1.09 0.50
85_D 253_L 1.089 0.50
85_D 89_H 1.088 0.50
74_G 271_G 1.087 0.50
65_A 173_I 1.086 0.50
69_W 88_H 1.084 0.50
87_I 119_P 1.078 0.49
147_I 159_L 1.074 0.49
87_I 183_L 1.067 0.48
282_Q 291_A 1.065 0.48
85_D 280_M 1.062 0.47
248_M 300_R 1.06 0.47
88_H 282_Q 1.059 0.47
119_P 122_G 1.054 0.47
205_N 231_T 1.053 0.46
82_G 215_N 1.052 0.46
85_D 278_K 1.051 0.46
116_C 122_G 1.048 0.46
46_F 52_V 1.041 0.45
93_L 249_W 1.041 0.45
202_K 288_R 1.034 0.45
79_P 285_K 1.033 0.45
295_M 303_F 1.023 0.44
77_C 223_L 1.021 0.43
130_F 183_L 1.021 0.43
79_P 218_G 1.02 0.43
136_S 151_P 1.018 0.43
180_D 248_M 1.017 0.43
285_K 289_F 1.016 0.43
85_D 202_K 1.014 0.43
120_L 183_L 1.013 0.43
192_A 220_Q 1.012 0.42
90_L 183_L 1.008 0.42
134_E 244_A 1.006 0.42
36_L 42_Q 1.004 0.42
90_L 226_E 1.004 0.42
147_I 239_E 1.002 0.41
17_R 116_C 1 0.41
166_G 235_K 1 0.41
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2h00A30.81821000.759Contact Map0.596
3evzA10.743599.90.828Contact Map0.666
2b3tA10.821499.20.894Contact Map0.435
1nv8A20.824799.20.895Contact Map0.455
4dzrA10.678699.20.896Contact Map0.384
2pjdA10.8019990.903Contact Map0.449
3lpmA20.7078990.903Contact Map0.565
2ozvA20.688398.90.908Contact Map0.528
3tm4A20.814998.80.908Contact Map0.581
3lduA10.785798.80.91Contact Map0.561
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
Page generated in 0.1051 seconds.