OPENSEQ.org - GRCA - ECOLI GREMLIN

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GRCA - Autonomous glycyl radical cofactor

UniProt:	P68066	Sequence MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVVAVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSGYAVRFNSLTPEQQRDVIARTFTESL	Download Alignment We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene:	EG11784
Length:	127 (109)
Sequences:	384
Seq/Len:	3.52

GRCA
Paralog alert: 0.58 [within 20: 0.01] - ratio of genomes with paralogs

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Scaled Score	Prob
85_D	89_H	3.374	1.00
80_R	84_E	2.99	1.00
87_V	112_P	2.573	1.00
85_D	92_K	2.459	1.00
89_H	92_K	2.426	1.00
87_V	116_R	2.297	1.00
112_P	116_R	2.059	1.00
17_N	68_E	1.93	1.00
39_A	72_H	1.884	1.00
103_Y	110_L	1.649	0.99
53_E	57_V	1.623	0.98
51_D	54_Y	1.621	0.98
85_D	93_Y	1.59	0.98
66_R	70_G	1.492	0.97
65_V	70_G	1.484	0.97
106_R	109_S	1.475	0.96
85_D	88_K	1.456	0.96
15_L	40_E	1.434	0.96
97_T	108_N	1.397	0.95
62_K	75_V	1.386	0.94
54_Y	57_V	1.37	0.94
21_L	95_Q	1.362	0.94
42_E	124_T	1.356	0.94
66_R	104_A	1.34	0.93
84_E	116_R	1.335	0.93
43_V	80_R	1.306	0.92
24_S	38_Y	1.284	0.91
16_L	73_L	1.271	0.90
43_V	122_T	1.26	0.90
71_Q	105_V	1.226	0.88
46_V	78_L	1.187	0.86
39_A	101_S	1.171	0.85
105_V	110_L	1.169	0.85
106_R	117_D	1.158	0.84
37_G	103_Y	1.157	0.84
52_I	79_R	1.151	0.83
88_K	92_K	1.148	0.83
21_L	104_A	1.144	0.83
83_L	96_L	1.124	0.81
41_D	101_S	1.122	0.81
95_Q	123_F	1.078	0.77
36_A	109_S	1.075	0.77
42_E	72_H	1.062	0.76
94_P	125_E	1.061	0.76
101_S	122_T	1.057	0.75
37_G	110_L	1.055	0.75
48_K	51_D	1.041	0.74
43_V	117_D	1.037	0.73
100_V	118_V	1.014	0.71
43_V	46_V	1.003	0.70

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling

HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:

Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	P(%)	HHΔ		Acc
2y8nA	2	0.9291	100	0.148	Contact Map	0.524
1r9dA	2	0.937	100	0.157	Contact Map	0.533
2f3oA	2	0.9291	100	0.162	Contact Map	0.461
1h16A	1	0.9449	100	0.184	Contact Map	0.584
3ajfA	4	0.7008	29.8	0.938	Contact Map	0.218
2kwvA	1	0.315	14.6	0.946	Contact Map	0.182
3ugsB	2	0.8504	11	0.949	Contact Map	0.132
1f75A	2	0.9055	9.5	0.951	Contact Map	0.064
4h8eA	1	0.9055	7.2	0.953	Contact Map	0.067
2yvyA	1	0.6457	6.2	0.955	Contact Map	0.064

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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