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OPENSEQ.org
LDHD - D-lactate dehydrogenase
UniProt: P52643 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13186
Length: 329 (318)
Sequences: 8183
Seq/Len: 25.73
LDHD
Paralog alert: 0.87 [within 20: 0.07] - ratio of genomes with paralogs
Cluster includes: GHRA GHRB LDHD SERA
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
216_N 219_A 4.798 1.00
239_D 242_A 3.999 1.00
217_E 245_E 3.733 1.00
225_N 251_K 2.861 1.00
147_A 163_L 2.846 1.00
192_P 222_Q 2.804 1.00
58_R 89_E 2.666 1.00
213_H 261_E 2.552 1.00
146_T 169_R 2.542 1.00
190_D 193_T 2.528 1.00
146_T 171_L 2.427 1.00
248_K 287_C 2.425 1.00
207_L 236_A 2.307 1.00
46_E 70_K 2.283 1.00
215_L 220_F 2.226 1.00
171_L 197_E 2.226 1.00
229_I 238_I 2.18 1.00
58_R 62_E 2.168 1.00
117_N 200_V 2.152 1.00
110_I 163_L 2.136 1.00
148_G 198_S 2.107 1.00
227_V 252_I 2.087 1.00
148_G 194_L 2.062 1.00
196_S 222_Q 2.051 1.00
195_F 219_A 2.038 1.00
119_R 122_R 2.034 1.00
221_E 251_K 2.016 1.00
124_Y 128_R 2.01 1.00
244_I 287_C 2.008 1.00
245_E 249_N 1.976 1.00
193_T 197_E 1.972 1.00
152_T 172_A 1.963 1.00
158_A 161_R 1.945 1.00
149_V 156_G 1.938 1.00
147_A 168_M 1.916 1.00
218_A 222_Q 1.904 1.00
199_D 224_K 1.903 1.00
110_I 166_F 1.891 1.00
228_M 254_S 1.89 1.00
108_H 297_A 1.868 1.00
44_G 66_K 1.86 1.00
243_A 255_L 1.831 1.00
113_M 200_V 1.83 1.00
111_G 120_I 1.822 1.00
317_N 322_E 1.813 1.00
287_C 290_V 1.811 1.00
213_H 239_D 1.807 1.00
209_P 212_Y 1.787 1.00
71_Y 95_V 1.767 1.00
62_E 90_L 1.763 1.00
220_F 246_A 1.757 1.00
195_F 215_L 1.747 1.00
145_K 302_E 1.732 1.00
116_L 254_S 1.714 1.00
163_L 168_M 1.714 1.00
217_E 221_E 1.711 1.00
203_L 237_L 1.703 1.00
169_R 187_E 1.684 1.00
115_T 121_H 1.633 1.00
192_P 219_A 1.591 1.00
215_L 219_A 1.566 1.00
159_M 204_H 1.563 1.00
228_M 253_G 1.563 1.00
107_E 162_I 1.559 1.00
220_F 229_I 1.556 1.00
61_L 90_L 1.535 1.00
207_L 212_Y 1.529 1.00
204_H 232_T 1.512 1.00
158_A 162_I 1.508 1.00
86_A 89_E 1.501 1.00
87_A 94_V 1.495 1.00
169_R 197_E 1.484 1.00
214_L 237_L 1.471 1.00
244_I 248_K 1.465 1.00
240_S 261_E 1.456 1.00
313_Q 317_N 1.45 1.00
202_S 232_T 1.447 1.00
112_M 256_G 1.446 1.00
149_V 160_L 1.433 1.00
217_E 242_A 1.431 1.00
171_L 189_V 1.426 1.00
149_V 202_S 1.419 1.00
205_C 237_L 1.418 1.00
61_L 72_I 1.411 1.00
150_I 201_I 1.406 0.99
85_D 89_E 1.405 0.99
58_R 86_A 1.39 0.99
47_A 71_Y 1.388 0.99
316_S 320_K 1.386 0.99
194_L 198_S 1.384 0.99
187_E 197_E 1.38 0.99
233_S 237_L 1.374 0.99
109_A 159_M 1.369 0.99
103_E 143_Y 1.357 0.99
111_G 114_M 1.355 0.99
219_A 222_Q 1.346 0.99
42_A 64_L 1.338 0.99
140_F 145_K 1.338 0.99
141_T 306_S 1.338 0.99
309_Q 313_Q 1.313 0.99
159_M 202_S 1.312 0.99
114_M 166_F 1.306 0.99
223_M 227_V 1.301 0.99
82_V 87_A 1.294 0.99
224_K 227_V 1.291 0.99
203_L 229_I 1.287 0.99
28_E 45_C 1.283 0.99
248_K 286_A 1.278 0.99
216_N 242_A 1.272 0.99
125_Q 129_D 1.267 0.99
248_K 283_R 1.265 0.99
317_N 325_P 1.252 0.99
201_I 223_M 1.25 0.99
192_P 218_A 1.238 0.98
86_A 90_L 1.236 0.98
212_Y 236_A 1.232 0.98
206_P 211_N 1.217 0.98
62_E 86_A 1.216 0.98
260_Y 292_F 1.213 0.98
61_L 87_A 1.204 0.98
74_L 82_V 1.203 0.98
150_I 205_C 1.202 0.98
101_D 298_F 1.202 0.98
161_R 167_G 1.194 0.98
108_H 112_M 1.184 0.98
116_L 291_L 1.183 0.98
57_S 60_V 1.181 0.98
152_T 157_V 1.179 0.98
103_E 158_A 1.179 0.98
109_A 232_T 1.169 0.97
93_K 327_E 1.168 0.97
150_I 215_L 1.147 0.97
150_I 214_L 1.144 0.97
74_L 81_N 1.115 0.96
116_L 228_M 1.113 0.96
171_L 198_S 1.111 0.96
73_A 314_N 1.11 0.96
106_A 162_I 1.11 0.96
200_V 228_M 1.107 0.96
108_H 293_T 1.105 0.96
318_L 324_C 1.102 0.96
189_V 194_L 1.098 0.96
46_E 66_K 1.086 0.96
116_L 256_G 1.084 0.96
126_R 131_N 1.082 0.96
216_N 239_D 1.077 0.95
215_L 229_I 1.069 0.95
191_L 195_F 1.067 0.95
226_G 252_I 1.062 0.95
76_C 81_N 1.048 0.94
71_Y 318_L 1.046 0.94
85_D 88_K 1.045 0.94
189_V 193_T 1.043 0.94
246_A 251_K 1.038 0.94
240_S 262_N 1.037 0.94
146_T 198_S 1.033 0.94
220_F 251_K 1.027 0.94
226_G 251_K 1.023 0.93
247_L 290_V 1.021 0.93
246_A 252_I 1.017 0.93
241_Q 245_E 1.016 0.93
191_L 219_A 1.015 0.93
198_S 201_I 1.008 0.93
112_M 291_L 1.006 0.93
247_L 289_N 1.003 0.93
240_S 279_D 1.002 0.92
48_V 56_G 1.001 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1dxyA10.98781000.182Contact Map0.74
1xdwA10.98781000.19Contact Map0.74
2g76A20.93621000.195Contact Map0.823
2yq5A40.98781000.197Contact Map0.823
1ygyA20.94531000.198Contact Map0.784
3k5pA10.94831000.201Contact Map0.769
2pi1A40.97871000.201Contact Map0.859
1j4aA40.98781000.202Contact Map0.789
1sc6A40.94831000.205Contact Map0.819
3ba1A10.93621000.211Contact Map0.752
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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