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OPENSEQ.org
RFAJ - Lipopolysaccharide 1,2-glucosyltransferase
UniProt: P27129 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11353
Length: 338 (297)
Sequences: 566
Seq/Len: 1.91
RFAJ
Paralog alert: 0.66 [within 20: 0.26] - ratio of genomes with paralogs
Cluster includes: RFAI RFAJ
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
109_M 181_S 5.373 1.00
32_Y 45_S 3.804 1.00
124_D 189_K 3.504 1.00
52_N 287_A 2.651 1.00
36_A 71_F 2.645 1.00
41_G 284_Y 2.6 1.00
127_L 185_Y 2.577 1.00
188_L 192_A 2.567 1.00
61_Y 89_Y 2.555 1.00
154_V 179_F 2.55 1.00
125_R 187_D 2.524 1.00
186_L 191_W 2.498 1.00
29_N 61_Y 2.378 1.00
46_I 60_F 2.306 1.00
109_M 214_P 2.276 1.00
181_S 214_P 2.238 1.00
49_I 60_F 2.156 0.99
179_F 219_M 2.151 0.99
44_V 134_V 2.104 0.99
31_A 115_A 2.103 0.99
147_L 150_A 2.071 0.99
29_N 59_D 2.038 0.99
59_D 87_T 1.934 0.98
178_Y 217_D 1.934 0.98
194_A 226_M 1.917 0.98
190_K 193_D 1.911 0.98
149_G 187_D 1.827 0.98
215_D 220_N 1.804 0.97
133_V 183_V 1.771 0.97
234_Y 261_L 1.7 0.96
137_G 261_L 1.695 0.96
151_V 187_D 1.684 0.96
134_V 265_Y 1.668 0.95
123_L 188_L 1.659 0.95
148_N 228_L 1.647 0.95
117_Q 197_T 1.645 0.95
129_L 183_V 1.634 0.94
147_L 153_A 1.626 0.94
34_V 131_A 1.622 0.94
135_C 262_L 1.595 0.93
195_K 198_E 1.594 0.93
120_G 192_A 1.592 0.93
35_D 108_A 1.504 0.91
203_I 221_V 1.504 0.91
53_N 138_D 1.503 0.91
198_E 202_S 1.488 0.90
196_L 199_K 1.459 0.89
183_V 262_L 1.454 0.89
43_G 293_W 1.451 0.88
31_A 126_L 1.449 0.88
150_A 187_D 1.432 0.87
136_K 263_I 1.406 0.86
45_S 133_V 1.405 0.86
49_I 135_C 1.393 0.85
105_W 214_P 1.389 0.85
178_Y 220_N 1.375 0.84
217_D 220_N 1.371 0.84
32_Y 49_I 1.37 0.84
214_P 217_D 1.318 0.81
147_L 187_D 1.299 0.80
190_K 194_A 1.295 0.79
200_A 218_V 1.292 0.79
215_D 219_M 1.276 0.78
55_H 139_I 1.273 0.78
29_N 123_L 1.263 0.77
120_G 188_L 1.243 0.75
136_K 283_Y 1.217 0.73
211_Y 217_D 1.214 0.73
202_S 205_M 1.207 0.72
34_V 42_V 1.207 0.72
187_D 190_K 1.203 0.72
317_Q 320_Y 1.202 0.72
218_V 222_L 1.201 0.72
129_L 135_C 1.198 0.71
96_L 114_F 1.187 0.70
191_W 196_L 1.186 0.70
151_V 194_A 1.174 0.69
308_K 311_Y 1.162 0.68
46_I 49_I 1.158 0.68
178_Y 215_D 1.146 0.66
49_I 139_I 1.142 0.66
112_R 128_Y 1.137 0.66
145_L 228_L 1.135 0.65
36_A 68_N 1.132 0.65
53_N 58_L 1.131 0.65
28_L 127_L 1.129 0.65
196_L 223_L 1.121 0.64
29_N 138_D 1.115 0.63
128_Y 215_D 1.114 0.63
151_V 226_M 1.111 0.63
110_Y 154_V 1.11 0.63
154_V 219_M 1.095 0.61
43_G 291_S 1.095 0.61
215_D 218_V 1.094 0.61
315_L 319_H 1.093 0.61
146_G 150_A 1.092 0.61
115_A 119_L 1.09 0.61
263_I 286_I 1.086 0.60
128_Y 178_Y 1.086 0.60
112_R 215_D 1.083 0.60
102_T 157_D 1.065 0.58
32_Y 42_V 1.061 0.58
58_L 123_L 1.055 0.57
233_E 261_L 1.052 0.57
51_L 82_N 1.049 0.56
124_D 172_P 1.036 0.55
36_A 39_L 1.035 0.55
35_D 110_Y 1.027 0.54
62_I 88_L 1.021 0.53
214_P 220_N 1.016 0.53
189_K 193_D 1.015 0.53
128_Y 220_N 1.012 0.52
300_D 303_S 1.009 0.52
306_E 314_L 1.008 0.52
265_Y 283_Y 1.008 0.52
152_A 184_V 1.001 0.51
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1g9rA10.78991000.409Contact Map0.748
3tztA20.69531000.428Contact Map0.697
3u2uA20.71011000.591Contact Map0.511
1ll2A10.69231000.601Contact Map0.511
2p6wA10.576991.90.943Contact Map0.387
3iohA10.677590.20.945Contact Map0.524
1o7qA20.707189.90.946Contact Map0.466
1xhbA10.863973.90.955Contact Map0.604
4mixA20.64555.60.96Contact Map0.492
2d7iA10.849138.70.964Contact Map0.663
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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