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OPENSEQ.org
MEND - 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
UniProt: P17109 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10579
Length: 556 (529)
Sequences: 6641
Seq/Len: 12.55
MEND
Paralog alert: 0.81 [within 20: 0.05] - ratio of genomes with paralogs
Cluster includes: GCL ILVB ILVG ILVI MEND OXC POXB
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
499_H 502_A 5.358 1.00
59_G 89_A 4.407 1.00
375_R 518_E 3.791 1.00
468_N 534_E 3.668 1.00
509_H 523_D 3.514 1.00
236_K 331_A 3.481 1.00
236_K 335_E 3.458 1.00
24_R 71_Q 3.448 1.00
513_N 516_E 3.321 1.00
375_R 378_D 2.929 1.00
510_R 536_V 2.916 1.00
226_A 237_V 2.831 1.00
328_A 333_W 2.822 1.00
285_L 330_I 2.784 1.00
512_Q 516_E 2.75 1.00
509_H 531_T 2.748 1.00
379_Y 522_A 2.743 1.00
68_V 432_S 2.643 1.00
279_A 282_V 2.63 1.00
511_P 520_A 2.522 1.00
309_V 333_W 2.511 1.00
25_H 48_I 2.507 1.00
242_Q 258_Q 2.502 1.00
509_H 520_A 2.487 1.00
507_K 529_T 2.483 1.00
446_L 504_F 2.483 1.00
507_K 523_D 2.452 1.00
101_I 156_L 2.432 1.00
214_W 283_V 2.391 1.00
77_V 86_L 2.356 1.00
445_A 449_L 2.285 1.00
151_T 166_I 2.232 1.00
425_A 438_A 2.229 1.00
378_D 518_E 2.2 1.00
19_T 46_A 2.195 1.00
63_L 67_K 2.147 1.00
72_P 101_I 2.125 1.00
210_K 307_W 2.12 1.00
84_A 88_P 2.118 1.00
500_A 503_M 2.118 1.00
224_V 241_A 2.106 1.00
90_L 102_L 2.097 1.00
241_A 248_L 2.072 1.00
456_R 506_L 2.072 1.00
496_H 499_H 2.07 1.00
518_E 522_A 2.068 1.00
12_A 40_A 2.066 1.00
510_R 534_E 2.01 1.00
52_H 58_L 1.997 1.00
389_G 392_L 1.996 1.00
69_S 431_A 1.979 1.00
466_V 532_V 1.93 1.00
124_G 127_A 1.915 1.00
285_L 309_V 1.912 1.00
429_Q 461_P 1.91 1.00
224_V 246_W 1.904 1.00
224_V 283_V 1.889 1.00
16_E 20_R 1.884 1.00
238_A 242_Q 1.87 1.00
138_R 141_Q 1.852 1.00
522_A 526_R 1.851 1.00
104_T 165_H 1.845 1.00
284_Q 287_S 1.84 1.00
12_A 43_E 1.836 1.00
524_A 533_I 1.835 1.00
103_L 168_C 1.829 1.00
16_E 19_T 1.825 1.00
121_R 124_G 1.822 1.00
498_E 508_Y 1.793 1.00
243_T 338_P 1.78 1.00
14_I 152_I 1.771 1.00
376_I 439_I 1.768 1.00
63_L 92_E 1.765 1.00
213_D 324_R 1.755 1.00
429_Q 435_P 1.74 1.00
90_L 100_L 1.71 1.00
235_K 239_L 1.706 1.00
410_Y 428_V 1.704 1.00
38_T 49_H 1.683 1.00
28_I 49_H 1.671 1.00
316_L 327_I 1.665 1.00
374_H 378_D 1.664 1.00
516_E 519_T 1.651 1.00
375_R 379_Y 1.648 1.00
455_L 506_L 1.644 1.00
416_S 419_D 1.631 1.00
385_Q 428_V 1.628 1.00
502_A 507_K 1.624 1.00
24_R 48_I 1.622 1.00
452_L 506_L 1.615 1.00
239_L 243_T 1.612 1.00
130_P 165_H 1.612 1.00
86_L 102_L 1.596 1.00
426_A 455_L 1.59 1.00
498_E 510_R 1.59 1.00
50_H 430_R 1.58 1.00
240_W 244_L 1.569 1.00
465_I 521_F 1.567 1.00
517_L 535_M 1.562 1.00
429_Q 460_A 1.554 1.00
519_T 522_A 1.55 1.00
435_P 525_W 1.546 1.00
519_T 523_D 1.544 1.00
152_I 166_I 1.542 1.00
465_I 533_I 1.537 1.00
391_S 394_V 1.529 1.00
134_I 166_I 1.526 1.00
371_Q 375_R 1.521 1.00
381_P 384_G 1.514 1.00
150_S 154_H 1.511 1.00
64_G 68_V 1.507 1.00
507_K 531_T 1.506 1.00
512_Q 536_V 1.485 1.00
420_G 424_T 1.484 1.00
21_H 153_D 1.479 1.00
317_D 322_R 1.476 1.00
223_V 279_A 1.468 1.00
219_Q 281_I 1.467 1.00
219_Q 222_G 1.457 1.00
281_I 307_W 1.457 1.00
239_L 338_P 1.451 1.00
371_Q 514_W 1.448 1.00
14_I 168_C 1.441 1.00
52_H 61_L 1.436 1.00
380_L 437_L 1.433 1.00
465_I 535_M 1.431 1.00
213_D 281_I 1.424 1.00
57_G 451_A 1.423 1.00
392_L 397_I 1.419 1.00
25_H 430_R 1.413 1.00
437_L 525_W 1.412 1.00
372_L 535_M 1.409 1.00
27_C 65_L 1.409 1.00
449_L 500_A 1.395 0.99
17_A 153_D 1.386 0.99
390_N 394_V 1.384 0.99
77_V 102_L 1.384 0.99
131_T 162_G 1.379 0.99
379_Y 518_E 1.375 0.99
455_L 462_L 1.374 0.99
214_W 281_I 1.366 0.99
83_V 104_T 1.364 0.99
48_I 458_V 1.355 0.99
21_H 156_L 1.354 0.99
390_N 393_V 1.354 0.99
261_P 264_D 1.353 0.99
212_R 218_R 1.352 0.99
509_H 533_I 1.35 0.99
34_S 37_L 1.342 0.99
348_E 352_L 1.334 0.99
371_Q 517_L 1.308 0.99
59_G 86_L 1.3 0.99
11_A 40_A 1.3 0.99
57_G 448_D 1.298 0.99
72_P 156_L 1.292 0.99
75_V 86_L 1.29 0.99
501_A 508_Y 1.289 0.99
211_Q 215_F 1.289 0.99
506_L 532_V 1.286 0.99
367_F 372_L 1.285 0.99
94_G 163_G 1.284 0.99
141_Q 150_S 1.283 0.99
209_E 212_R 1.28 0.99
136_L 148_L 1.269 0.99
278_Q 348_E 1.264 0.99
222_G 280_Q 1.26 0.99
520_A 533_I 1.26 0.99
12_A 16_E 1.258 0.99
207_E 340_E 1.257 0.99
50_H 458_V 1.257 0.99
9_R 12_A 1.256 0.99
517_L 521_F 1.25 0.99
239_L 335_E 1.249 0.99
224_V 240_W 1.247 0.99
213_D 305_E 1.247 0.99
109_P 171_A 1.246 0.98
452_L 532_V 1.245 0.98
237_V 334_L 1.244 0.98
416_S 420_G 1.242 0.98
391_S 395_R 1.24 0.98
367_F 535_M 1.239 0.98
384_G 435_P 1.229 0.98
332_D 335_E 1.226 0.98
285_L 334_L 1.223 0.98
87_Y 125_M 1.22 0.98
239_L 242_Q 1.218 0.98
438_A 464_L 1.218 0.98
511_P 516_E 1.215 0.98
59_G 75_V 1.214 0.98
463_V 524_A 1.213 0.98
211_Q 337_H 1.212 0.98
408_P 434_K 1.201 0.98
17_A 152_I 1.2 0.98
130_P 133_S 1.196 0.98
508_Y 532_V 1.194 0.98
415_A 419_D 1.191 0.98
331_A 335_E 1.187 0.98
326_L 333_W 1.175 0.98
415_A 418_I 1.169 0.97
445_A 532_V 1.169 0.97
19_T 44_N 1.166 0.97
453_A 457_Q 1.164 0.97
210_K 213_D 1.161 0.97
23_V 74_A 1.157 0.97
524_A 531_T 1.156 0.97
461_P 529_T 1.155 0.97
236_K 334_L 1.153 0.97
475_F 478_L 1.151 0.97
348_E 351_R 1.149 0.97
501_A 506_L 1.148 0.97
240_W 338_P 1.137 0.97
236_K 239_L 1.135 0.97
221_R 278_Q 1.132 0.97
350_P 353_A 1.131 0.97
13_V 149_V 1.127 0.97
134_I 151_T 1.124 0.97
223_V 282_V 1.12 0.96
59_G 100_L 1.119 0.96
507_K 527_T 1.113 0.96
224_V 237_V 1.113 0.96
417_G 420_G 1.111 0.96
169_P 172_E 1.109 0.96
90_L 129_H 1.105 0.96
498_E 502_A 1.104 0.96
430_R 460_A 1.104 0.96
511_P 517_L 1.101 0.96
381_P 435_P 1.099 0.96
389_G 393_V 1.099 0.96
224_V 285_L 1.095 0.96
391_S 396_L 1.092 0.96
236_K 338_P 1.091 0.96
390_N 397_I 1.091 0.96
394_V 397_I 1.082 0.96
498_E 534_E 1.079 0.95
347_V 351_R 1.068 0.95
392_L 395_R 1.068 0.95
134_I 154_H 1.061 0.95
14_I 103_L 1.06 0.95
343_Q 347_V 1.058 0.95
347_V 350_P 1.054 0.95
101_I 159_L 1.052 0.95
66_A 99_K 1.05 0.94
158_T 164_V 1.044 0.94
449_L 504_F 1.042 0.94
260_L 263_A 1.042 0.94
429_Q 436_T 1.041 0.94
219_Q 280_Q 1.04 0.94
87_Y 129_H 1.038 0.94
125_M 129_H 1.037 0.94
429_Q 433_G 1.037 0.94
75_V 102_L 1.033 0.94
443_L 472_G 1.033 0.94
19_T 45_S 1.032 0.94
500_A 504_F 1.031 0.94
61_L 455_L 1.029 0.94
470_N 494_N 1.017 0.93
26_I 41_A 1.014 0.93
351_R 355_Q 1.013 0.93
516_E 520_A 1.012 0.93
254_S 257_G 1.012 0.93
501_A 532_V 1.012 0.93
235_K 238_A 1.01 0.93
210_K 326_L 1.009 0.93
392_L 396_L 1.007 0.93
523_D 526_R 1.005 0.93
234_G 238_A 1.003 0.93
65_L 431_A 1.003 0.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3hwwA20.99281000.123Contact Map0.714
2x7jA40.98561000.133Contact Map0.702
3lq1A20.98381000.146Contact Map0.734
1ozhA40.95861000.15Contact Map0.81
1ybhA10.96941000.156Contact Map0.798
2ihtA40.9551000.158Contact Map0.807
2panA60.96221000.161Contact Map0.687
1t9bA20.97661000.163Contact Map0.803
3eyaA120.94961000.164Contact Map0.785
2uz1A20.95321000.17Contact Map0.763
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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