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OPENSEQ.org
INSH8 - Transposase InsH for insertion sequence element IS5K
UniProt: P0CE55 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG60038
Length: 338 (318)
Sequences: 1103
Seq/Len: 3.47
INSH8
Paralog alert: 0.48 [within 20: 0.08] - ratio of genomes with paralogs
Cluster includes: INH10 INH11 INSH1 INSH2 INSH3 INSH4 INSH5 INSH6 INSH7 INSH8 INSH9
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
263_L 272_T 3.951 1.00
266_H 272_T 2.963 1.00
195_V 287_V 2.884 1.00
263_L 266_H 2.756 1.00
92_A 96_I 2.666 1.00
87_G 114_R 2.66 1.00
205_L 316_A 2.651 1.00
192_H 208_T 2.574 1.00
75_I 93_L 2.526 1.00
202_T 319_F 2.341 1.00
242_E 246_E 2.264 1.00
70_E 74_R 2.166 1.00
79_Q 89_M 2.091 1.00
52_V 132_Q 2.004 1.00
244_L 249_V 1.991 1.00
93_L 103_A 1.979 1.00
208_T 217_Q 1.888 1.00
115_T 118_M 1.879 1.00
58_P 62_N 1.835 0.99
307_G 310_K 1.802 0.99
95_E 98_S 1.785 0.99
305_Y 310_K 1.785 0.99
204_S 224_G 1.745 0.99
16_Q 19_F 1.736 0.99
195_V 290_P 1.719 0.99
136_T 139_R 1.711 0.99
179_T 190_K 1.709 0.99
157_T 295_K 1.68 0.99
294_I 318_L 1.659 0.99
191_A 315_L 1.629 0.98
201_L 326_R 1.614 0.98
160_E 302_K 1.583 0.98
152_T 194_G 1.546 0.97
17_L 20_A 1.537 0.97
205_L 319_F 1.537 0.97
86_D 121_R 1.529 0.97
244_L 253_I 1.501 0.97
115_T 119_N 1.483 0.96
88_A 92_A 1.474 0.96
176_M 189_M 1.472 0.96
189_M 308_L 1.467 0.96
50_V 70_E 1.463 0.96
322_A 326_R 1.461 0.96
75_I 117_I 1.452 0.96
194_G 204_S 1.419 0.95
156_A 190_K 1.419 0.95
263_L 273_A 1.4 0.95
72_M 120_F 1.388 0.94
31_T 38_S 1.386 0.94
50_V 68_P 1.368 0.94
131_R 135_K 1.366 0.94
90_E 117_I 1.329 0.92
18_T 21_D 1.325 0.92
102_F 187_F 1.322 0.92
237_G 240_Q 1.316 0.92
291_F 295_K 1.313 0.92
105_L 109_S 1.305 0.92
201_L 327_A 1.294 0.91
218_L 240_Q 1.287 0.91
114_R 118_M 1.285 0.91
228_F 252_L 1.283 0.91
231_A 253_I 1.278 0.90
193_I 291_F 1.256 0.89
223_H 243_E 1.256 0.89
24_F 27_K 1.254 0.89
160_E 186_H 1.253 0.89
100_R 111_L 1.251 0.89
212_E 217_Q 1.249 0.89
78_M 82_Y 1.245 0.89
41_E 74_R 1.243 0.89
303_A 311_N 1.242 0.89
296_R 301_V 1.242 0.89
38_S 101_L 1.238 0.88
134_F 319_F 1.231 0.88
137_I 141_L 1.222 0.88
152_T 203_H 1.222 0.88
192_H 221_L 1.221 0.87
49_M 53_I 1.217 0.87
207_T 312_D 1.213 0.87
133_L 320_T 1.212 0.87
80_H 314_Q 1.211 0.87
179_T 210_A 1.198 0.86
17_L 21_D 1.195 0.86
20_A 23_E 1.187 0.85
281_A 284_R 1.187 0.85
79_Q 92_A 1.187 0.85
266_H 269_K 1.183 0.85
192_H 235_Y 1.183 0.85
193_I 290_P 1.179 0.85
139_R 143_E 1.179 0.85
67_Y 109_S 1.178 0.85
37_L 102_F 1.169 0.84
196_D 199_S 1.166 0.84
183_N 186_H 1.161 0.84
76_H 121_R 1.161 0.84
204_S 221_L 1.16 0.84
223_H 226_E 1.158 0.83
219_G 243_E 1.156 0.83
303_A 314_Q 1.15 0.83
75_I 112_P 1.143 0.82
90_E 113_D 1.14 0.82
71_T 105_L 1.129 0.81
86_D 120_F 1.126 0.81
305_Y 311_N 1.124 0.81
141_L 327_A 1.122 0.81
74_R 103_A 1.121 0.81
54_E 59_K 1.117 0.80
78_M 99_M 1.108 0.80
156_A 192_H 1.106 0.79
75_I 103_A 1.105 0.79
232_D 235_Y 1.097 0.79
154_V 194_G 1.093 0.78
57_Y 123_L 1.08 0.77
153_L 286_R 1.074 0.77
256_R 268_R 1.072 0.76
323_N 326_R 1.069 0.76
326_R 329_Q 1.065 0.76
75_I 89_M 1.063 0.76
321_L 325_F 1.053 0.75
124_L 284_R 1.05 0.74
300_F 318_L 1.047 0.74
234_G 237_G 1.047 0.74
305_Y 314_Q 1.045 0.74
286_R 289_H 1.044 0.74
259_K 263_L 1.043 0.74
267_P 270_N 1.041 0.73
92_A 99_M 1.037 0.73
19_F 22_S 1.036 0.73
190_K 214_D 1.035 0.73
158_I 179_T 1.035 0.73
163_S 172_R 1.031 0.72
72_M 116_T 1.025 0.72
246_E 261_R 1.023 0.71
298_F 325_F 1.023 0.71
131_R 134_F 1.023 0.71
156_A 178_Q 1.023 0.71
72_M 231_A 1.021 0.71
78_M 102_F 1.02 0.71
16_Q 21_D 1.02 0.71
206_V 312_D 1.019 0.71
47_Q 50_V 1.018 0.71
162_P 306_K 1.013 0.70
195_V 202_T 1.013 0.70
153_L 197_A 1.012 0.70
86_D 118_M 1.012 0.70
238_A 242_E 1.005 0.70
192_H 210_A 1.005 0.70
67_Y 71_T 1.004 0.69
156_A 291_F 1.001 0.69
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1musA10.78797.10.919Contact Map0.547
3hotA20.822564.20.956Contact Map0.645
1bcoA10.49729.60.965Contact Map0.429
3kksA20.405329.10.965Contact Map0.622
4fw1A20.446726.20.966Contact Map0.6
1jkoC10.150922.50.967Contact Map0.661
1c6vA40.420122.20.967Contact Map0.632
4dyqA20.390521.50.967Contact Map0.46
3l3uA20.4201150.969Contact Map0.641
1ex4A20.420114.10.97Contact Map0.554
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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