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OPENSEQ.org
RIBF - Riboflavin biosynthesis protein RibF
UniProt: P0AG40 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11079
Length: 313 (307)
Sequences: 1784
Seq/Len: 5.81
RIBF
Paralog alert: 0.01 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
227_V 241_A 3.913 1.00
197_H 200_E 3.445 1.00
177_N 180_L 3.211 1.00
287_A 291_E 2.915 1.00
79_L 90_V 2.865 1.00
179_A 278_K 2.863 1.00
178_L 182_E 2.837 1.00
262_D 304_R 2.817 1.00
279_K 282_N 2.793 1.00
201_L 249_V 2.77 1.00
227_V 258_V 2.541 1.00
229_V 272_I 2.528 1.00
144_G 151_I 2.484 1.00
128_R 136_D 2.404 1.00
227_V 239_G 2.402 1.00
242_N 283_E 2.339 1.00
240_V 296_I 2.304 1.00
241_A 258_V 2.303 1.00
40_Q 44_R 2.202 1.00
38_G 152_T 2.177 1.00
200_E 203_R 2.17 1.00
50_V 88_D 2.153 1.00
300_E 304_R 2.059 1.00
136_D 139_L 2.041 1.00
109_S 114_K 2.03 1.00
159_E 164_I 1.98 1.00
118_V 121_L 1.974 1.00
120_F 152_T 1.92 1.00
42_E 45_K 1.919 1.00
120_F 150_D 1.914 1.00
52_V 87_V 1.898 1.00
42_E 46_R 1.862 1.00
240_V 303_A 1.859 1.00
233_G 270_R 1.855 1.00
93_V 99_F 1.844 1.00
229_V 260_L 1.838 1.00
229_V 239_G 1.83 1.00
210_A 267_L 1.826 1.00
226_A 306_F 1.824 1.00
144_G 149_F 1.812 1.00
20_L 53_M 1.81 1.00
228_E 275_V 1.793 1.00
293_K 297_A 1.788 1.00
105_Q 146_E 1.778 1.00
53_M 93_V 1.774 1.00
22_I 127_F 1.764 1.00
20_L 118_V 1.762 1.00
108_I 143_A 1.75 1.00
162_V 168_A 1.727 1.00
261_L 304_R 1.712 1.00
280_I 302_T 1.71 1.00
38_G 41_E 1.642 1.00
226_A 277_R 1.636 1.00
199_D 255_Q 1.597 0.99
228_E 238_P 1.591 0.99
249_V 289_L 1.582 0.99
240_V 300_E 1.574 0.99
106_N 110_D 1.571 0.99
75_L 92_C 1.55 0.99
36_L 85_C 1.537 0.99
110_D 114_K 1.535 0.99
137_F 153_S 1.531 0.99
239_G 258_V 1.528 0.99
104_A 134_E 1.526 0.99
168_A 184_L 1.525 0.99
260_L 267_L 1.513 0.99
263_V 267_L 1.507 0.99
43_G 50_V 1.506 0.99
280_I 303_A 1.494 0.99
51_M 91_L 1.483 0.99
261_L 303_A 1.468 0.99
101_A 131_A 1.431 0.98
207_F 297_A 1.412 0.98
277_R 307_F 1.409 0.98
103_T 106_N 1.408 0.98
125_D 156_T 1.395 0.98
288_S 291_E 1.39 0.98
277_R 306_F 1.386 0.98
190_A 273_Q 1.366 0.98
99_F 102_L 1.36 0.98
190_A 275_V 1.35 0.97
239_G 261_L 1.317 0.97
224_V 242_N 1.316 0.97
97_R 101_A 1.313 0.97
297_A 300_E 1.307 0.97
228_E 277_R 1.304 0.97
301_L 305_E 1.303 0.97
19_V 122_A 1.301 0.97
207_F 300_E 1.3 0.97
172_A 180_L 1.292 0.96
199_D 246_R 1.282 0.96
18_C 51_M 1.275 0.96
40_Q 50_V 1.272 0.96
61_E 216_R 1.256 0.96
191_I 276_L 1.254 0.96
48_L 119_K 1.254 0.96
73_T 81_Y 1.253 0.96
294_A 298_R 1.242 0.95
77_E 217_Q 1.241 0.95
298_R 301_L 1.226 0.95
261_L 300_E 1.216 0.95
6_G 9_N 1.205 0.94
171_Q 175_D 1.2 0.94
182_E 188_P 1.195 0.94
108_I 149_F 1.191 0.94
62_L 96_D 1.19 0.94
139_L 143_A 1.186 0.94
201_L 204_T 1.183 0.93
278_K 306_F 1.176 0.93
298_R 302_T 1.175 0.93
34_A 37_Q 1.172 0.93
157_F 164_I 1.167 0.93
210_A 272_I 1.166 0.93
77_E 192_S 1.163 0.93
243_I 256_L 1.157 0.92
193_G 212_V 1.154 0.92
221_V 279_K 1.148 0.92
295_Q 299_D 1.146 0.92
50_V 87_V 1.143 0.92
238_P 261_L 1.139 0.92
54_L 90_V 1.138 0.92
212_V 258_V 1.135 0.91
296_I 300_E 1.131 0.91
205_I 289_L 1.125 0.91
289_L 293_K 1.122 0.91
129_F 139_L 1.116 0.90
105_Q 109_S 1.116 0.90
135_G 139_L 1.116 0.90
192_S 273_Q 1.103 0.90
178_L 276_L 1.103 0.90
49_P 89_Y 1.101 0.90
228_E 236_P 1.096 0.89
242_N 284_Q 1.095 0.89
187_H 190_A 1.093 0.89
192_S 271_H 1.09 0.89
169_V 184_L 1.075 0.88
51_M 89_Y 1.067 0.88
195_V 272_I 1.064 0.87
172_A 177_N 1.064 0.87
33_R 37_Q 1.063 0.87
21_T 39_L 1.052 0.87
4_I 89_Y 1.049 0.86
281_R 295_Q 1.048 0.86
207_F 242_N 1.047 0.86
33_R 187_H 1.046 0.86
230_L 275_V 1.044 0.86
41_E 45_K 1.042 0.86
241_A 256_L 1.042 0.86
82_L 90_V 1.04 0.86
17_G 119_K 1.031 0.85
222_K 283_E 1.028 0.85
128_R 133_R 1.023 0.85
138_L 142_K 1.017 0.84
49_P 88_D 1.014 0.84
188_P 277_R 1.01 0.84
201_L 289_L 1.009 0.83
232_L 270_R 1.007 0.83
108_I 113_V 1.005 0.83
75_L 79_L 1.005 0.83
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2x0kA20.99041000.149Contact Map0.74
3op1A30.95851000.178Contact Map0.809
1mrzA20.90421000.199Contact Map0.842
3bnwA20.45051000.585Contact Map0.618
1n08A20.46961000.593Contact Map0.682
1nb0A10.40891000.597Contact Map0.66
3covA20.771000.674Contact Map0.376
2ejcA10.74441000.701Contact Map0.406
3hl4A20.55591000.711Contact Map0.338
3uk2A20.74761000.712Contact Map0.421
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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