May 4, 2021 - We are working on upgrading the webserver, some pages may not work.
OPENSEQ.org
DAUA - C4-dicarboxylic acid transporter DauA
UniProt: P0AFR2 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12392
Length: 559 (537)
Sequences: 3275
Seq/Len: 6.10
DAUA
Paralog alert: 0.45 [within 20: 0.03] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
75_T 319_L 3.782 1.00
120_G 315_V 3.468 1.00
110_L 325_P 3.443 1.00
33_L 355_I 3.344 1.00
181_I 186_A 3.113 1.00
155_F 189_G 2.97 1.00
71_V 323_I 2.829 1.00
416_S 420_L 2.757 1.00
125_G 311_N 2.692 1.00
342_N 352_S 2.511 1.00
177_A 180_T 2.466 1.00
182_N 185_D 2.443 1.00
193_L 381_A 2.4 1.00
29_F 33_L 2.391 1.00
71_V 319_L 2.367 1.00
115_F 279_L 2.341 1.00
118_L 121_L 2.293 1.00
324_A 329_G 2.265 1.00
417_L 429_V 2.253 1.00
198_F 202_L 2.246 1.00
111_L 282_A 2.231 1.00
89_F 285_M 2.228 1.00
192_T 381_A 2.212 1.00
188_I 230_V 2.211 1.00
523_V 526_V 2.167 1.00
70_I 354_V 2.107 1.00
512_V 521_L 2.097 1.00
75_T 316_G 2.087 1.00
459_V 491_I 2.081 1.00
193_L 385_M 2.041 1.00
90_V 330_I 2.037 1.00
47_A 86_T 2 1.00
118_L 122_A 1.986 1.00
300_L 339_S 1.984 1.00
149_T 171_V 1.956 1.00
56_V 330_I 1.928 1.00
489_I 522_R 1.916 1.00
413_L 433_L 1.909 1.00
551_N 554_A 1.908 1.00
114_I 118_L 1.901 1.00
355_I 359_L 1.896 1.00
494_W 497_V 1.874 1.00
458_D 488_R 1.855 1.00
36_G 355_I 1.835 1.00
197_V 385_M 1.808 1.00
70_I 357_S 1.798 1.00
64_T 332_A 1.794 1.00
110_L 322_I 1.737 1.00
526_V 530_P 1.726 1.00
119_M 124_F 1.718 1.00
112_S 285_M 1.71 1.00
473_A 503_G 1.694 1.00
67_V 324_A 1.689 1.00
109_T 331_T 1.629 1.00
323_I 327_F 1.623 1.00
67_V 327_F 1.596 0.99
304_T 343_V 1.579 0.99
28_R 348_T 1.579 0.99
362_L 366_V 1.562 0.99
117_I 322_I 1.55 0.99
24_Y 32_D 1.544 0.99
422_D 425_I 1.537 0.99
279_L 283_F 1.536 0.99
172_G 176_M 1.533 0.99
25_T 28_R 1.525 0.99
487_K 519_C 1.52 0.99
185_D 377_S 1.514 0.99
120_G 125_G 1.492 0.99
523_V 548_F 1.489 0.99
85_P 338_R 1.488 0.99
21_K 24_Y 1.479 0.99
84_G 338_R 1.466 0.99
249_G 253_L 1.465 0.99
156_L 174_L 1.462 0.99
29_F 355_I 1.461 0.99
192_T 212_A 1.461 0.99
459_V 489_I 1.459 0.99
193_L 197_V 1.458 0.99
219_V 223_V 1.453 0.99
24_Y 29_F 1.451 0.99
206_L 211_P 1.437 0.99
112_S 331_T 1.422 0.98
417_L 421_F 1.421 0.98
187_A 191_V 1.416 0.98
145_I 419_V 1.412 0.98
156_L 178_L 1.407 0.98
211_P 214_L 1.404 0.98
72_I 83_S 1.396 0.98
128_I 131_I 1.395 0.98
216_G 378_A 1.394 0.98
218_A 221_G 1.391 0.98
32_D 348_T 1.391 0.98
412_L 416_S 1.387 0.98
71_V 75_T 1.381 0.98
543_P 546_L 1.38 0.98
296_C 336_I 1.37 0.98
465_I 496_A 1.368 0.98
110_L 326_F 1.361 0.98
522_R 549_F 1.358 0.98
62_L 360_V 1.358 0.98
80_F 343_V 1.35 0.98
426_A 430_G 1.349 0.98
189_G 381_A 1.349 0.98
480_L 511_F 1.349 0.98
418_T 426_A 1.343 0.98
35_A 39_V 1.342 0.98
207_P 211_P 1.34 0.98
354_V 358_I 1.337 0.98
113_G 322_I 1.332 0.97
271_T 274_S 1.324 0.97
234_G 372_S 1.323 0.97
175_F 178_L 1.316 0.97
528_F 532_R 1.315 0.97
440_R 444_R 1.313 0.97
68_A 332_A 1.312 0.97
144_G 387_A 1.31 0.97
35_A 345_A 1.308 0.97
539_I 543_P 1.304 0.97
531_L 535_A 1.303 0.97
467_P 498_P 1.298 0.97
115_F 286_A 1.297 0.97
301_D 306_T 1.292 0.97
342_N 347_A 1.285 0.97
552_R 556_M 1.281 0.97
145_I 167_Y 1.278 0.96
341_A 345_A 1.276 0.96
217_C 220_M 1.276 0.96
218_A 222_I 1.272 0.96
67_V 323_I 1.272 0.96
115_F 282_A 1.271 0.96
74_L 354_V 1.27 0.96
40_G 86_T 1.27 0.96
320_G 324_A 1.261 0.96
66_A 361_I 1.26 0.96
273_D 276_R 1.259 0.96
523_V 534_M 1.258 0.96
491_I 522_R 1.257 0.96
42_I 341_A 1.254 0.96
276_R 279_L 1.254 0.96
468_L 503_G 1.253 0.96
80_F 347_A 1.253 0.96
482_S 485_E 1.251 0.96
121_L 315_V 1.249 0.96
347_A 352_S 1.249 0.96
108_A 278_L 1.249 0.96
183_V 187_A 1.231 0.95
285_M 331_T 1.224 0.95
541_P 544_G 1.223 0.95
413_L 429_V 1.218 0.95
512_V 516_P 1.218 0.95
184_G 187_A 1.218 0.95
186_A 190_I 1.217 0.95
292_E 333_T 1.214 0.95
108_A 282_A 1.209 0.95
154_D 380_A 1.208 0.95
148_G 384_L 1.207 0.95
540_Q 548_F 1.205 0.95
73_A 353_A 1.204 0.95
463_R 496_A 1.199 0.94
196_L 381_A 1.19 0.94
142_G 419_V 1.186 0.94
363_A 367_L 1.181 0.94
191_V 222_I 1.18 0.94
226_L 229_H 1.178 0.94
50_L 337_A 1.165 0.93
176_M 215_A 1.164 0.93
544_G 547_A 1.162 0.93
315_V 319_L 1.158 0.93
83_S 317_Q 1.157 0.93
535_A 545_R 1.157 0.93
549_F 554_A 1.157 0.93
73_A 354_V 1.156 0.93
27_A 30_T 1.156 0.93
282_A 286_A 1.154 0.93
414_C 430_G 1.153 0.93
532_R 535_A 1.152 0.93
36_G 40_G 1.149 0.93
223_V 226_L 1.147 0.93
188_I 191_V 1.143 0.92
312_S 315_V 1.14 0.92
120_G 318_G 1.139 0.92
46_L 143_I 1.139 0.92
155_F 380_A 1.137 0.92
140_T 390_M 1.134 0.92
542_I 545_R 1.13 0.92
358_I 362_L 1.129 0.92
222_I 271_T 1.129 0.92
550_P 554_A 1.128 0.92
299_V 303_M 1.123 0.92
192_T 216_G 1.122 0.92
53_G 86_T 1.122 0.92
272_W 275_I 1.114 0.91
37_I 359_L 1.114 0.91
82_V 353_A 1.113 0.91
477_F 510_R 1.111 0.91
490_V 515_L 1.106 0.91
68_A 324_A 1.105 0.91
538_G 541_P 1.103 0.91
435_S 439_M 1.102 0.91
80_F 304_T 1.102 0.91
363_A 371_L 1.1 0.90
31_R 348_T 1.1 0.90
192_T 378_A 1.099 0.90
461_V 491_I 1.098 0.90
203_G 206_L 1.095 0.90
515_L 519_C 1.095 0.90
68_A 72_I 1.094 0.90
69_G 357_S 1.092 0.90
144_G 391_S 1.089 0.90
290_A 314_L 1.088 0.90
29_F 351_I 1.086 0.90
130_Y 469_F 1.083 0.89
335_A 339_S 1.082 0.89
56_V 60_Y 1.081 0.89
82_V 88_A 1.078 0.89
273_D 277_T 1.075 0.89
357_S 361_I 1.074 0.89
301_D 308_H 1.072 0.89
224_N 227_G 1.072 0.89
117_I 121_L 1.069 0.89
486_G 519_C 1.069 0.89
96_V 100_F 1.068 0.89
136_T 298_V 1.067 0.89
50_L 87_A 1.065 0.88
32_D 349_S 1.063 0.88
197_V 388_W 1.06 0.88
89_F 112_S 1.06 0.88
300_L 343_V 1.054 0.88
141_S 144_G 1.052 0.88
72_I 75_T 1.052 0.88
335_A 338_R 1.051 0.88
210_L 214_L 1.048 0.87
155_F 384_L 1.047 0.87
166_H 169_Q 1.044 0.87
121_L 124_F 1.043 0.87
549_F 555_A 1.041 0.87
293_S 334_A 1.041 0.87
115_F 283_F 1.039 0.87
71_V 316_G 1.039 0.87
40_G 359_L 1.037 0.87
168_L 420_L 1.035 0.86
111_L 278_L 1.034 0.86
289_G 333_T 1.034 0.86
143_I 295_L 1.032 0.86
337_A 340_A 1.031 0.86
53_G 88_A 1.028 0.86
189_G 384_L 1.027 0.86
529_Q 532_R 1.026 0.86
26_A 29_F 1.024 0.86
86_T 335_A 1.022 0.86
109_T 325_P 1.022 0.86
400_L 408_I 1.02 0.85
504_G 507_A 1.02 0.85
242_A 245_S 1.017 0.85
86_T 336_I 1.016 0.85
137_L 392_E 1.013 0.85
140_T 338_R 1.011 0.85
543_P 547_A 1.009 0.85
114_I 322_I 1.009 0.85
361_I 365_L 1.009 0.85
513_K 516_P 1.008 0.84
309_K 312_S 1.006 0.84
44_I 360_V 1.004 0.84
29_F 319_L 1.004 0.84
199_W 211_P 1.002 0.84
73_A 468_L 1.002 0.84
77_G 82_V 1.001 0.84
41_I 142_G 1 0.84
283_F 323_I 1 0.84
365_L 369_P 1 0.84
42_I 46_L 1 0.84
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3qe7A10.7121000.673Contact Map0.467
4dghA20.205799.70.872Contact Map0.711
3lloA10.207599.70.873Contact Map0.722
4dgfA20.209399.70.873Contact Map0.86
2klnA10.207599.70.873Contact Map0.435
3ny7A10.211199.70.874Contact Map0.656
2ka5A10.209399.50.888Contact Map0.713
1h4xA20.191499.50.89Contact Map0.637
1th8B10.19599.50.89Contact Map0.745
4hylA20.198699.40.892Contact Map0.81
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
Page generated in 0.0164 seconds.