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OPENSEQ.org

FLIA - RNA polymerase sigma factor for flagellar operon
UniProt: P0AEM6 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11355
Length: 239 (228)
Sequences: 3537
Seq/Len: 15.51

FLIA
Paralog alert: 0.76 [within 20: 0.08] - ratio of genomes with paralogs
Cluster includes: FLIA RPOH RPOS
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
107_L 111_L 3.97 1.00
194_K 198_T 3.689 1.00
110_E 123_R 3.613 1.00
113_R 119_E 3.536 1.00
118_T 128_I 3.344 1.00
24_V 48_G 3.321 1.00
13_K 57_E 3.046 1.00
216_V 221_V 2.962 1.00
14_H 18_Q 2.757 1.00
106_Q 123_R 2.629 1.00
103_A 123_R 2.567 1.00
220_R 223_Q 2.537 1.00
195_L 213_V 2.496 1.00
199_L 213_V 2.299 1.00
200_Y 225_H 2.122 1.00
193_E 231_R 2.116 1.00
214_L 221_V 2.084 1.00
188_T 235_K 2.056 1.00
23_L 68_T 2.018 1.00
199_L 205_L 2.013 1.00
187_E 194_K 1.962 1.00
196_V 228_A 1.914 1.00
106_Q 110_E 1.893 1.00
120_V 131_Y 1.892 1.00
60_D 63_Q 1.879 1.00
15_S 19_R 1.815 1.00
100_V 134_M 1.802 1.00
208_K 212_A 1.782 1.00
21_V 49_G 1.764 1.00
170_L 174_L 1.761 1.00
196_V 224_L 1.727 1.00
17_W 53_L 1.718 1.00
190_P 231_R 1.715 1.00
127_D 130_D 1.71 1.00
219_S 222_S 1.681 1.00
117_E 135_L 1.668 1.00
103_A 106_Q 1.662 1.00
118_T 122_E 1.637 1.00
107_L 115_A 1.617 1.00
195_L 199_L 1.6 1.00
195_L 214_L 1.595 1.00
107_L 113_R 1.548 1.00
100_V 120_V 1.528 1.00
117_E 132_R 1.527 1.00
117_E 131_Y 1.516 1.00
56_V 67_F 1.497 1.00
116_T 119_E 1.472 1.00
121_A 128_I 1.47 1.00
58_R 70_Y 1.462 1.00
28_A 45_L 1.449 1.00
27_E 72_V 1.425 1.00
205_L 213_V 1.406 0.99
28_A 79_M 1.4 0.99
117_E 128_I 1.4 0.99
191_E 194_K 1.385 0.99
111_L 115_A 1.349 0.99
207_L 211_G 1.342 0.99
103_A 124_L 1.339 0.99
103_A 107_L 1.315 0.99
219_S 223_Q 1.3 0.99
95_R 99_E 1.299 0.99
34_R 38_S 1.289 0.99
67_F 71_A 1.285 0.99
27_E 79_M 1.284 0.99
47_A 82_E 1.28 0.99
100_V 124_L 1.261 0.99
66_A 69_T 1.261 0.99
104_I 135_L 1.249 0.99
128_I 132_R 1.246 0.98
126_I 134_M 1.234 0.98
33_V 38_S 1.229 0.98
29_L 41_L 1.213 0.98
191_E 195_L 1.212 0.98
55_A 71_A 1.21 0.98
45_L 49_G 1.208 0.98
96_N 126_I 1.207 0.98
28_A 41_L 1.204 0.98
197_L 232_L 1.195 0.98
89_V 97_A 1.17 0.97
51_G 55_A 1.169 0.97
107_L 123_R 1.16 0.97
23_L 69_T 1.16 0.97
200_Y 210_I 1.132 0.97
103_A 120_V 1.126 0.97
220_R 224_L 1.126 0.97
207_L 222_S 1.103 0.96
121_A 131_Y 1.094 0.96
25_R 45_L 1.093 0.96
207_L 219_S 1.08 0.95
20_Y 68_T 1.079 0.95
121_A 124_L 1.076 0.95
16_L 56_V 1.069 0.95
208_K 218_E 1.055 0.95
207_L 218_E 1.052 0.95
121_A 126_I 1.047 0.94
205_L 209_E 1.022 0.93
96_N 134_M 1.018 0.93
76_R 80_L 1.014 0.93
179_R 183_M 1.012 0.93
102_Q 106_Q 1.007 0.93
77_G 84_R 1.001 0.92
48_G 79_M 1 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2a6hF20.97911000.283Contact Map0.541
1l9zH10.97911000.284Contact Map0.061
4kn7X20.97911000.342Contact Map0.624
4ljzF20.97911000.345Contact Map0.634
1rp3A40.97911000.45Contact Map0.747
1l0oC10.92471000.495Contact Map0.745
3ugoA10.5999.90.608Contact Map0.841
3mzyA10.661199.80.683Contact Map0.639
3vdoA10.72899.80.686Contact Map0.641
1or7A20.74999.80.687Contact Map0.654

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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