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OPENSEQ.org
MPRA - Transcriptional repressor MprA
UniProt: P0ACR9 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10603
Length: 176 (145)
Sequences: 5686
Seq/Len: 39.21
MPRA
Paralog alert: 0.79 [within 20: 0.06] - ratio of genomes with paralogs
Cluster includes: MARR MPRA SLYA
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
147_E 157_K 3.469 1.00
71_S 116_Q 3.12 1.00
144_S 147_E 2.962 1.00
104_R 116_Q 2.738 1.00
102_E 116_Q 2.526 1.00
106_S 114_H 2.434 1.00
68_E 71_S 2.308 1.00
150_Q 154_I 2.287 1.00
143_L 154_I 2.229 1.00
142_A 161_R 2.216 1.00
65_E 125_L 2.117 1.00
93_D 110_R 2.041 1.00
150_Q 153_Q 1.975 1.00
74_P 92_A 1.96 1.00
151_L 154_I 1.837 1.00
105_E 113_L 1.767 1.00
106_S 112_C 1.747 1.00
57_L 98_R 1.738 1.00
120_K 123_E 1.707 1.00
147_E 153_Q 1.654 1.00
96_E 103_R 1.637 1.00
73_Q 112_C 1.634 1.00
123_E 127_E 1.567 1.00
65_E 117_L 1.557 1.00
99_G 120_K 1.555 1.00
67_Q 80_A 1.542 1.00
120_K 124_F 1.495 1.00
32_L 156_R 1.482 1.00
154_I 157_K 1.453 1.00
93_D 97_K 1.446 1.00
36_M 155_T 1.44 1.00
92_A 101_I 1.403 0.99
89_T 93_D 1.4 0.99
73_Q 76_E 1.394 0.99
57_L 94_E 1.378 0.99
147_E 150_Q 1.348 0.99
36_M 159_L 1.348 0.99
74_P 89_T 1.315 0.99
51_Q 131_P 1.313 0.99
71_S 114_H 1.292 0.99
71_S 104_R 1.288 0.99
54_N 57_L 1.274 0.99
153_Q 157_K 1.27 0.99
147_E 154_I 1.252 0.99
74_P 113_L 1.246 0.98
35_H 159_L 1.246 0.98
76_E 79_C 1.224 0.98
61_L 125_L 1.22 0.98
92_A 115_L 1.214 0.98
52_G 98_R 1.19 0.98
32_L 152_E 1.189 0.98
119_E 122_H 1.173 0.98
51_Q 127_E 1.167 0.97
100_W 124_F 1.165 0.97
107_D 114_H 1.161 0.97
119_E 123_E 1.144 0.97
63_T 80_A 1.142 0.97
45_N 49_K 1.138 0.97
65_E 122_H 1.131 0.97
47_M 138_Q 1.112 0.96
47_M 131_P 1.109 0.96
32_L 159_L 1.105 0.96
56_T 91_I 1.104 0.96
84_S 90_R 1.099 0.96
39_K 159_L 1.094 0.96
96_E 102_E 1.085 0.96
64_L 101_I 1.077 0.95
124_F 127_E 1.075 0.95
143_L 158_L 1.062 0.95
37_Q 41_L 1.045 0.94
60_A 88_A 1.036 0.94
117_L 121_G 1.032 0.94
58_F 132_Q 1.019 0.93
95_L 100_W 1.013 0.93
98_R 120_K 1.012 0.93
152_E 156_R 1.001 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2fbkA20.943299.90.356Contact Map0.766
3deuA20.931899.90.404Contact Map0.612
4em2A10.943299.90.41Contact Map0.526
3e6mA80.909199.90.42Contact Map0.868
3cjnA10.903499.90.424Contact Map0.591
3u2rA10.948999.90.428Contact Map0.643
3zmdA40.960299.90.433Contact Map0.789
3bj6A20.863699.90.436Contact Map0.692
3bpvA10.784199.90.44Contact Map0.572
4l9nA20.823999.90.44Contact Map0.699
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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