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OPENSEQ.org
GLMU - Bifunctional protein GlmU
UniProt: P0ACC7 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11198
Length: 456 (450)
Sequences: 2135
Seq/Len: 4.74
GLMU
Paralog alert: 0.13 [within 20: 0.01] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
298_I 301_S 4.429 1.00
143_T 165_I 3.628 1.00
300_N 317_D 3.58 1.00
371_E 394_K 3.535 1.00
340_E 357_K 3.435 1.00
151_G 162_Q 3.12 1.00
155_H 163_R 3.114 1.00
375_N 400_D 2.954 1.00
398_G 417_K 2.873 1.00
355_L 361_A 2.691 1.00
350_M 353_A 2.687 1.00
132_K 166_Q 2.57 1.00
121_K 127_G 2.466 1.00
228_N 249_L 2.286 1.00
338_L 342_A 2.283 1.00
359_S 399_D 2.279 1.00
301_S 315_V 2.266 1.00
33_K 41_D 2.254 1.00
336_A 350_M 2.204 1.00
160_D 163_R 2.197 1.00
247_L 268_H 2.167 1.00
289_R 306_D 2.145 1.00
22_D 289_R 2.139 1.00
267_T 285_T 2.131 1.00
145_E 148_K 2.102 1.00
132_K 216_H 2.085 1.00
361_A 367_L 2.061 1.00
135_D 155_H 2.054 1.00
186_K 208_E 2.044 1.00
149_V 214_A 2.031 1.00
119_D 123_Q 2.017 1.00
400_D 418_G 2.015 1.00
322_A 340_E 1.997 1.00
23_L 29_T 1.977 1.00
113_T 222_E 1.965 1.00
245_E 249_L 1.955 1.00
247_L 250_A 1.935 1.00
99_I 177_G 1.932 1.00
439_S 442_P 1.927 1.00
339_L 357_K 1.926 1.00
288_H 305_D 1.916 1.00
407_T 421_I 1.909 1.00
307_C 340_E 1.89 1.00
287_G 305_D 1.888 1.00
83_G 187_L 1.886 1.00
80_L 84_H 1.88 1.00
110_S 222_E 1.878 1.00
8_V 48_A 1.87 1.00
137_T 155_H 1.869 1.00
159_T 162_Q 1.868 1.00
355_L 367_L 1.857 1.00
304_G 322_A 1.851 1.00
298_I 303_I 1.835 1.00
182_R 208_E 1.78 1.00
90_A 185_A 1.77 1.00
226_V 235_L 1.77 1.00
272_V 290_V 1.762 1.00
396_I 414_T 1.756 1.00
277_N 295_G 1.705 0.99
437_A 445_Q 1.68 0.99
143_T 151_G 1.672 0.99
50_H 72_N 1.653 0.99
415_V 421_I 1.64 0.99
84_H 88_Q 1.636 0.99
361_A 365_T 1.633 0.99
376_V 399_D 1.629 0.99
295_G 312_Y 1.625 0.99
432_G 435_A 1.611 0.99
280_I 284_V 1.609 0.99
269_G 288_H 1.607 0.99
374_D 399_D 1.603 0.99
56_G 79_Q 1.602 0.99
357_K 374_D 1.596 0.99
307_C 324_C 1.588 0.99
416_G 433_E 1.586 0.99
98_D 122_P 1.58 0.99
417_K 433_E 1.574 0.99
320_L 324_C 1.565 0.99
353_A 367_L 1.563 0.99
294_T 311_P 1.548 0.99
265_T 283_N 1.541 0.99
156_K 192_A 1.54 0.99
319_N 337_E 1.538 0.99
128_L 149_V 1.536 0.99
315_V 318_A 1.535 0.99
306_D 323_A 1.533 0.99
337_E 354_R 1.527 0.99
384_T 397_I 1.525 0.99
352_K 394_K 1.522 0.99
344_V 350_M 1.519 0.98
101_M 175_A 1.515 0.98
230_L 249_L 1.505 0.98
269_G 290_V 1.498 0.98
414_T 430_N 1.488 0.98
318_A 336_A 1.487 0.98
40_I 51_V 1.479 0.98
418_G 434_N 1.477 0.98
138_G 155_H 1.476 0.98
385_C 408_Q 1.475 0.98
428_T 440_R 1.472 0.98
432_G 447_E 1.472 0.98
403_V 409_L 1.457 0.98
376_V 417_K 1.453 0.98
305_D 322_A 1.449 0.98
269_G 287_G 1.447 0.98
408_Q 426_T 1.443 0.98
429_R 442_P 1.435 0.98
79_Q 84_H 1.423 0.97
399_D 417_K 1.422 0.97
55_Y 59_G 1.422 0.97
270_R 288_H 1.417 0.97
52_H 74_V 1.407 0.97
372_I 378_I 1.403 0.97
272_V 305_D 1.4 0.97
179_D 210_R 1.4 0.97
431_V 437_A 1.393 0.97
8_V 43_A 1.393 0.97
350_M 367_L 1.392 0.97
269_G 305_D 1.378 0.97
342_A 356_G 1.376 0.97
266_L 280_I 1.37 0.97
343_H 362_G 1.367 0.96
413_V 427_V 1.36 0.96
332_L 336_A 1.36 0.96
126_I 205_A 1.356 0.96
435_A 447_E 1.356 0.96
80_L 196_Y 1.355 0.96
128_L 142_I 1.354 0.96
36_V 66_L 1.352 0.96
286_L 290_V 1.35 0.96
335_G 352_K 1.34 0.96
272_V 287_G 1.335 0.96
42_A 111_V 1.329 0.96
90_A 181_K 1.327 0.96
331_R 346_N 1.325 0.96
148_K 211_E 1.32 0.95
243_Q 247_L 1.32 0.95
410_V 428_T 1.316 0.95
56_G 84_H 1.315 0.95
175_A 201_I 1.305 0.95
144_R 167_E 1.305 0.95
355_L 359_S 1.295 0.95
299_K 316_E 1.293 0.95
43_A 102_L 1.285 0.95
303_I 309_I 1.28 0.94
320_L 332_L 1.277 0.94
59_G 75_L 1.275 0.94
161_E 164_Q 1.267 0.94
244_A 254_L 1.26 0.94
230_L 245_E 1.254 0.94
371_E 396_I 1.254 0.94
338_L 344_V 1.251 0.93
289_R 305_D 1.238 0.93
183_W 204_L 1.231 0.93
86_M 101_M 1.227 0.93
307_C 321_A 1.223 0.92
59_G 63_K 1.221 0.92
321_A 337_E 1.219 0.92
354_R 371_E 1.218 0.92
182_R 186_K 1.214 0.92
281_E 299_K 1.211 0.92
61_L 64_Q 1.197 0.91
425_T 439_S 1.197 0.91
55_Y 73_W 1.194 0.91
44_N 70_N 1.193 0.91
428_T 439_S 1.187 0.91
408_Q 439_S 1.181 0.91
145_E 150_T 1.181 0.91
18_R 25_K 1.18 0.91
255_R 273_E 1.16 0.90
406_D 439_S 1.159 0.90
323_A 341_G 1.157 0.89
103_Y 106_V 1.152 0.89
302_V 319_N 1.149 0.89
350_M 361_A 1.147 0.89
303_I 307_C 1.144 0.89
401_V 419_A 1.143 0.89
438_I 441_V 1.141 0.89
203_A 207_Q 1.14 0.88
229_R 253_M 1.137 0.88
117_L 127_G 1.135 0.88
233_S 257_P 1.135 0.88
355_L 362_G 1.135 0.88
97_E 177_G 1.133 0.88
427_V 437_A 1.133 0.88
353_A 370_A 1.133 0.88
269_G 272_V 1.132 0.88
376_V 401_V 1.127 0.88
314_V 329_F 1.124 0.88
321_A 340_E 1.119 0.87
359_S 376_V 1.116 0.87
177_G 181_K 1.112 0.87
415_V 419_A 1.112 0.87
84_H 87_Q 1.103 0.86
37_Q 41_D 1.102 0.86
246_K 250_A 1.102 0.86
269_G 289_R 1.101 0.86
98_D 174_I 1.097 0.86
27_L 65_A 1.097 0.86
273_E 291_K 1.095 0.86
103_Y 173_L 1.095 0.86
26_V 104_G 1.093 0.85
130_T 167_E 1.091 0.85
42_A 45_E 1.09 0.85
40_I 71_L 1.086 0.85
55_Y 63_K 1.084 0.85
434_N 448_G 1.079 0.84
152_I 199_T 1.076 0.84
382_T 407_T 1.075 0.84
339_L 354_R 1.07 0.84
187_L 199_T 1.069 0.84
16_G 25_K 1.056 0.83
130_T 214_A 1.054 0.83
287_G 290_V 1.052 0.82
312_Y 329_F 1.052 0.82
385_C 402_F 1.052 0.82
324_C 339_L 1.051 0.82
44_N 51_V 1.045 0.82
9_V 86_M 1.045 0.82
439_S 445_Q 1.044 0.82
133_L 168_I 1.043 0.82
434_N 451_R 1.039 0.81
131_V 168_I 1.039 0.81
324_C 342_A 1.035 0.81
35_M 226_V 1.027 0.80
356_G 359_S 1.024 0.80
419_A 433_E 1.021 0.80
303_I 315_V 1.021 0.80
237_R 258_A 1.016 0.79
330_A 344_V 1.012 0.79
324_C 340_E 1.006 0.78
400_D 417_K 1.005 0.78
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4fceA111000.365Contact Map0.696
1hm9A20.99121000.37Contact Map0.721
4g3qA10.99781000.371Contact Map0.736
3st8A10.99781000.371Contact Map0.731
2v0hA111000.372Contact Map0.719
2ggoA10.82461000.51Contact Map0.719
1yp2A40.83771000.551Contact Map0.551
3brkX10.8181000.57Contact Map0.638
3r3iA40.65571000.614Contact Map0.445
2i5kA20.67981000.636Contact Map0.481
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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