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OPENSEQ.org
GFCE - Putative polysaccharide export protein GfcE
UniProt: P0A932 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13728
Length: 379 (373)
Sequences: 463
Seq/Len: 1.24
GFCE
Paralog alert: 0.36 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: GFCE WZA
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
87_I 144_V 5.914 1.00
87_I 120_V 3.631 1.00
136_V 144_V 2.402 0.99
54_P 317_Q 2.342 0.99
139_K 147_E 2.235 0.99
297_G 346_P 2.128 0.98
364_V 370_T 1.862 0.95
18_L 21_C 1.809 0.94
21_C 25_P 1.791 0.94
194_I 247_L 1.718 0.92
86_R 143_Q 1.67 0.90
229_L 280_E 1.669 0.90
148_I 244_G 1.661 0.90
121_N 125_A 1.657 0.89
256_K 280_E 1.627 0.88
195_M 238_N 1.619 0.88
297_G 343_T 1.584 0.86
299_F 316_Y 1.574 0.86
174_Y 199_N 1.544 0.84
9_S 12_T 1.54 0.84
241_L 247_L 1.535 0.84
99_D 336_P 1.517 0.83
197_A 241_L 1.477 0.81
6_F 10_V 1.465 0.80
55_V 59_L 1.447 0.79
163_V 316_Y 1.438 0.78
105_T 277_T 1.437 0.78
298_I 344_T 1.437 0.78
120_V 186_P 1.434 0.78
281_A 334_L 1.432 0.78
139_K 144_V 1.415 0.77
12_T 15_V 1.412 0.76
131_I 158_S 1.407 0.76
372_R 376_T 1.395 0.75
144_V 163_V 1.394 0.75
262_E 352_R 1.39 0.75
153_D 335_E 1.383 0.74
11_L 143_Q 1.383 0.74
279_A 329_G 1.365 0.73
149_T 161_V 1.363 0.73
263_V 342_V 1.363 0.73
85_Y 101_P 1.344 0.71
127_F 145_R 1.343 0.71
5_I 9_S 1.335 0.71
225_N 240_L 1.322 0.69
103_L 106_P 1.316 0.69
294_D 319_N 1.316 0.69
350_W 354_I 1.311 0.69
300_V 318_L 1.308 0.68
12_T 16_L 1.307 0.68
364_V 372_R 1.304 0.68
182_S 283_G 1.296 0.67
134_L 198_I 1.289 0.67
351_N 355_S 1.288 0.67
11_L 14_A 1.272 0.65
20_A 301_I 1.271 0.65
275_G 346_P 1.261 0.64
269_L 276_M 1.258 0.64
55_V 318_L 1.248 0.63
86_R 140_T 1.246 0.63
198_I 223_K 1.243 0.63
293_A 306_N 1.24 0.62
97_V 318_L 1.235 0.62
217_Q 242_H 1.231 0.61
148_I 163_V 1.226 0.61
1_M 7_K 1.226 0.61
365_H 369_E 1.214 0.60
100_H 191_P 1.212 0.60
95_V 148_I 1.208 0.59
50_V 315_I 1.207 0.59
118_N 354_I 1.202 0.59
137_A 323_A 1.196 0.58
289_N 293_A 1.195 0.58
81_A 221_K 1.193 0.58
146_N 150_A 1.188 0.57
372_R 375_Q 1.187 0.57
88_G 170_S 1.187 0.57
54_P 319_N 1.185 0.57
362_S 368_T 1.181 0.57
182_S 248_F 1.176 0.56
7_K 10_V 1.175 0.56
326_M 353_V 1.173 0.56
370_T 373_W 1.166 0.55
98_W 158_S 1.156 0.54
365_H 373_W 1.154 0.54
204_L 229_L 1.154 0.54
165_V 259_V 1.152 0.54
63_L 136_V 1.147 0.53
130_Y 253_D 1.147 0.53
8_F 33_N 1.146 0.53
144_V 147_E 1.144 0.53
140_T 240_L 1.131 0.52
6_F 9_S 1.13 0.52
112_S 240_L 1.13 0.52
366_D 372_R 1.13 0.52
88_G 278_L 1.129 0.52
242_H 294_D 1.129 0.52
6_F 12_T 1.124 0.51
104_T 108_G 1.122 0.51
154_S 158_S 1.119 0.51
11_L 15_V 1.116 0.50
59_L 259_V 1.115 0.50
5_I 13_L 1.115 0.50
294_D 321_K 1.106 0.49
52_I 55_V 1.103 0.49
182_S 200_A 1.102 0.49
33_N 375_Q 1.099 0.49
26_G 35_D 1.099 0.49
52_I 298_I 1.095 0.48
60_I 328_L 1.09 0.48
339_I 347_L 1.087 0.47
215_L 271_M 1.085 0.47
143_Q 163_V 1.08 0.47
252_N 340_V 1.077 0.46
364_V 373_W 1.075 0.46
10_V 288_M 1.073 0.46
300_V 332_F 1.072 0.46
140_T 143_Q 1.072 0.46
93_L 112_S 1.072 0.46
293_A 347_L 1.07 0.46
5_I 10_V 1.069 0.46
277_T 291_D 1.069 0.46
177_G 201_A 1.065 0.45
85_Y 259_V 1.061 0.45
216_T 221_K 1.057 0.45
92_V 159_P 1.054 0.44
369_E 372_R 1.052 0.44
256_K 284_N 1.049 0.44
88_G 100_H 1.049 0.44
86_R 171_Q 1.049 0.44
353_V 357_L 1.048 0.44
97_V 100_H 1.047 0.44
89_I 288_M 1.047 0.44
226_L 294_D 1.044 0.43
10_V 14_A 1.043 0.43
99_D 169_R 1.042 0.43
234_D 237_Q 1.04 0.43
167_A 249_I 1.038 0.43
80_I 131_I 1.035 0.42
179_V 203_G 1.035 0.42
52_I 283_G 1.034 0.42
194_I 224_V 1.033 0.42
344_T 352_R 1.032 0.42
288_M 298_I 1.031 0.42
104_T 145_R 1.027 0.42
108_G 198_I 1.026 0.42
108_G 111_R 1.025 0.41
56_T 87_I 1.025 0.41
343_T 346_P 1.024 0.41
142_T 213_V 1.023 0.41
102_E 300_V 1.022 0.41
134_L 148_I 1.021 0.41
290_Q 344_T 1.021 0.41
185_Q 244_G 1.021 0.41
109_Q 114_S 1.017 0.41
104_T 235_L 1.017 0.41
6_F 376_T 1.016 0.41
193_T 279_A 1.014 0.40
12_T 17_S 1.014 0.40
56_T 228_A 1.013 0.40
258_F 280_E 1.012 0.40
131_I 151_R 1.012 0.40
149_T 315_I 1.012 0.40
145_R 318_L 1.012 0.40
216_T 237_Q 1.011 0.40
6_F 33_N 1.009 0.40
347_L 356_L 1.008 0.40
95_V 364_V 1.007 0.40
6_F 14_A 1.006 0.40
114_S 213_V 1.006 0.40
85_Y 209_D 1.005 0.40
103_L 233_G 1.005 0.40
275_G 297_G 1.004 0.39
28_N 341_Y 1.003 0.39
87_I 142_T 1.003 0.39
105_T 111_R 1.003 0.39
41_D 45_D 1.001 0.39
199_N 258_F 1 0.39
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2j58A80.94721000.29Contact Map0.465
3p42A40.48021000.73Contact Map0.192
3zbiC140.126635.90.971Contact Map0
3mb5A10.345622.70.973Contact Map0.182
1i9gA10.361521.50.974Contact Map0.186
3abfA60.15319.10.974Contact Map0.431
3ej9A30.184715.60.975Contact Map0.393
3m20A30.150414.20.976Contact Map0.398
2kzfA10.234811.10.977Contact Map0.191
2pwyA20.356210.10.977Contact Map0.058
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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