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OPENSEQ.org
TOLB - Protein TolB
UniProt: P0A855 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11008
Length: 430 (412)
Sequences: 633
Seq/Len: 1.54
TOLB
Paralog alert: 0.01 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
61_D 149_S 4.134 1.00
272_V 295_W 3.771 1.00
303_A 314_V 3.32 1.00
154_E 160_K 3.01 1.00
237_R 278_G 2.61 1.00
168_A 214_L 2.591 1.00
349_V 358_I 2.574 1.00
167_I 185_S 2.387 1.00
269_N 286_G 2.229 0.99
100_V 152_V 2.156 0.99
283_V 317_V 2.09 0.99
136_V 144_A 1.975 0.98
220_E 236_V 1.961 0.98
318_N 324_P 1.942 0.98
169_Y 192_Q 1.937 0.98
309_A 315_Y 1.934 0.98
286_G 290_N 1.903 0.97
274_D 277_S 1.886 0.97
318_N 322_G 1.879 0.97
320_N 324_P 1.842 0.96
159_I 387_T 1.84 0.96
256_S 276_A 1.806 0.96
357_H 377_D 1.755 0.95
257_K 272_V 1.755 0.95
265_T 282_Q 1.732 0.94
331_G 334_N 1.711 0.94
302_L 319_I 1.682 0.93
274_D 279_Q 1.669 0.93
91_W 99_V 1.571 0.90
87_Q 90_A 1.569 0.90
261_A 268_L 1.565 0.90
129_L 156_L 1.538 0.88
261_A 293_P 1.537 0.88
316_K 339_V 1.532 0.88
61_D 146_H 1.522 0.88
269_N 290_N 1.513 0.87
313_Q 326_R 1.508 0.87
215_A 258_L 1.508 0.87
142_R 188_D 1.505 0.87
204_S 217_V 1.486 0.86
256_S 322_G 1.482 0.86
283_V 302_L 1.467 0.85
291_T 385_N 1.457 0.84
304_F 308_Q 1.457 0.84
263_S 268_L 1.449 0.84
54_I 137_N 1.448 0.84
117_Q 131_Q 1.445 0.84
296_F 339_V 1.444 0.84
251_F 275_L 1.432 0.83
381_S 390_I 1.428 0.83
259_A 272_V 1.428 0.83
265_T 271_Y 1.427 0.83
347_V 360_K 1.42 0.82
76_L 80_P 1.419 0.82
54_I 58_V 1.418 0.82
346_M 363_L 1.416 0.82
259_A 293_P 1.41 0.82
201_P 219_F 1.4 0.81
372_S 377_D 1.397 0.81
303_A 327_I 1.382 0.80
204_S 249_P 1.375 0.79
269_N 285_D 1.371 0.79
77_P 90_A 1.355 0.78
183_R 194_V 1.351 0.78
328_T 369_Q 1.339 0.77
282_Q 290_N 1.332 0.76
301_N 319_I 1.331 0.76
203_M 224_S 1.329 0.76
369_Q 372_S 1.325 0.76
143_Y 164_R 1.324 0.76
103_Q 115_A 1.32 0.75
259_A 295_W 1.309 0.75
146_H 188_D 1.305 0.74
345_F 360_K 1.303 0.74
58_V 145_G 1.302 0.74
339_V 346_M 1.293 0.73
346_M 364_A 1.291 0.73
315_Y 405_S 1.286 0.73
228_I 239_V 1.263 0.71
328_T 359_A 1.261 0.71
33_S 314_V 1.26 0.71
257_K 274_D 1.256 0.70
242_F 273_M 1.243 0.69
56_G 60_A 1.228 0.68
164_R 187_Y 1.227 0.68
132_N 151_E 1.226 0.68
383_A 401_L 1.224 0.67
209_P 255_G 1.222 0.67
60_A 64_N 1.22 0.67
196_H 339_V 1.216 0.67
272_V 319_I 1.213 0.66
269_N 282_Q 1.212 0.66
39_V 81_G 1.208 0.66
245_H 427_S 1.205 0.66
295_W 319_I 1.199 0.65
292_E 305_T 1.198 0.65
146_H 186_D 1.198 0.65
247_G 291_T 1.196 0.65
307_D 321_G 1.192 0.64
159_I 389_V 1.191 0.64
213_K 237_R 1.187 0.64
298_D 385_N 1.185 0.64
360_K 371_L 1.185 0.64
274_D 278_G 1.182 0.63
312_P 333_Q 1.18 0.63
239_V 258_L 1.179 0.63
77_P 89_A 1.179 0.63
39_V 425_A 1.171 0.62
161_G 165_T 1.169 0.62
328_T 361_Q 1.167 0.62
301_N 325_Q 1.165 0.62
359_A 370_V 1.16 0.61
96_I 149_S 1.16 0.61
296_F 301_N 1.158 0.61
244_R 268_L 1.155 0.61
330_E 347_V 1.152 0.60
218_T 227_V 1.15 0.60
344_K 364_A 1.143 0.60
170_V 202_L 1.143 0.60
230_T 235_A 1.14 0.59
392_S 424_P 1.14 0.59
340_S 382_L 1.138 0.59
116_Y 148_A 1.132 0.58
226_L 260_F 1.131 0.58
213_K 230_T 1.131 0.58
58_V 61_D 1.127 0.58
160_K 429_Y 1.126 0.58
26_V 29_S 1.125 0.58
284_T 317_V 1.12 0.57
302_L 317_V 1.115 0.57
350_S 359_A 1.112 0.56
227_V 238_Q 1.112 0.56
22_E 25_I 1.111 0.56
104_V 114_V 1.109 0.56
71_L 96_I 1.104 0.56
185_S 223_R 1.101 0.55
82_S 344_K 1.097 0.55
239_V 338_D 1.094 0.55
29_S 427_S 1.094 0.55
162_A 388_M 1.093 0.54
231_L 413_R 1.09 0.54
346_M 417_T 1.088 0.54
237_R 276_A 1.088 0.54
330_E 334_N 1.087 0.54
27_I 295_W 1.085 0.54
272_V 427_S 1.084 0.54
98_A 156_L 1.079 0.53
196_H 382_L 1.078 0.53
246_N 312_P 1.076 0.53
90_A 94_L 1.074 0.52
267_S 286_G 1.071 0.52
142_R 146_H 1.07 0.52
153_F 387_T 1.068 0.52
346_M 361_Q 1.068 0.52
346_M 365_T 1.067 0.52
358_I 370_V 1.066 0.52
301_N 316_K 1.066 0.52
391_Y 411_K 1.065 0.52
126_G 396_G 1.065 0.52
246_N 262_L 1.064 0.51
305_T 312_P 1.063 0.51
388_M 405_S 1.061 0.51
289_N 308_Q 1.06 0.51
405_S 409_R 1.059 0.51
246_N 263_S 1.053 0.50
118_L 165_T 1.047 0.50
23_V 226_L 1.047 0.50
257_K 281_R 1.046 0.50
317_V 321_G 1.045 0.49
361_Q 366_G 1.036 0.49
63_R 70_P 1.033 0.48
144_A 152_V 1.028 0.48
327_I 337_A 1.028 0.48
100_V 208_S 1.026 0.47
326_R 329_W 1.025 0.47
89_A 270_L 1.025 0.47
58_V 62_L 1.024 0.47
303_A 339_V 1.024 0.47
321_G 324_P 1.02 0.47
270_L 312_P 1.019 0.47
150_D 161_G 1.016 0.46
342_D 363_L 1.016 0.46
21_A 119_V 1.015 0.46
99_V 119_V 1.015 0.46
242_F 262_L 1.014 0.46
233_N 277_S 1.01 0.46
400_V 421_V 1.009 0.46
202_L 291_T 1.004 0.45
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2hqsA40.94651000.486Contact Map0.629
3pe7A10.6071000.729Contact Map0.396
4hvtA10.75581000.732Contact Map0.141
1z68A20.64881000.739Contact Map0.425
4a5sA20.65351000.74Contact Map0.404
1xfdA40.55351000.741Contact Map0.37
2ecfA10.6861000.741Contact Map0.551
3c5mA30.59771000.744Contact Map0.38
3s25A10.6071000.751Contact Map0.463
4hxfB10.61161000.757Contact Map0.521
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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