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OPENSEQ.org
PURA - Adenylosuccinate synthetase
UniProt: P0A7D4 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10790
Length: 432 (430)
Sequences: 1646
Seq/Len: 3.83
PURA
Paralog alert: 0.04 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
341_E 375_T 6.578 1.00
349_R 370_E 6.381 1.00
54_H 301_T 6.136 1.00
340_K 380_S 4.347 1.00
388_D 391_G 4.146 1.00
85_K 197_I 4.043 1.00
394_Q 398_N 3.873 1.00
52_V 300_A 3.698 1.00
352_D 368_G 3.507 1.00
330_L 400_I 3.387 1.00
292_C 305_R 3.224 1.00
374_E 402_R 3.148 1.00
389_R 397_L 3.071 1.00
49_E 90_R 2.974 1.00
93_P 96_E 2.857 1.00
289_E 293_K 2.831 1.00
185_Q 189_D 2.811 1.00
402_R 406_L 2.771 1.00
344_L 402_R 2.727 1.00
183_D 186_K 2.584 1.00
76_S 112_V 2.58 1.00
341_E 377_P 2.567 1.00
290_F 294_Q 2.566 1.00
85_K 89_D 2.505 1.00
344_L 376_M 2.501 1.00
290_F 339_L 2.491 1.00
395_A 398_N 2.429 1.00
106_L 149_V 2.36 1.00
349_R 355_E 2.309 1.00
343_K 375_T 2.28 1.00
313_T 406_L 2.235 1.00
30_Y 212_A 2.233 1.00
311_L 316_V 2.233 1.00
32_V 253_S 2.222 1.00
337_D 383_T 2.206 1.00
116_N 131_G 2.187 1.00
338_G 381_E 2.184 1.00
350_M 369_V 2.104 1.00
389_R 401_K 2.102 1.00
211_Q 215_R 2.034 1.00
78_A 190_D 2.02 1.00
159_A 163_K 2.012 1.00
401_K 426_L 1.978 1.00
153_F 202_V 1.973 1.00
328_F 407_T 1.964 1.00
350_M 368_G 1.951 1.00
262_Y 327_G 1.933 1.00
381_E 395_A 1.904 1.00
228_L 315_A 1.891 1.00
398_N 401_K 1.888 1.00
344_L 406_L 1.886 1.00
126_A 276_A 1.866 1.00
29_K 217_D 1.865 1.00
214_Q 408_G 1.861 1.00
327_G 412_D 1.829 1.00
394_Q 397_L 1.829 1.00
376_M 402_R 1.803 0.99
110_Y 165_V 1.764 0.99
293_K 298_F 1.74 0.99
292_C 298_F 1.734 0.99
196_D 199_T 1.733 0.99
149_V 202_V 1.719 0.99
397_L 401_K 1.715 0.99
69_I 73_V 1.691 0.99
162_L 184_Y 1.687 0.99
75_L 104_C 1.678 0.99
174_V 181_A 1.674 0.99
377_P 395_A 1.669 0.99
10_T 325_L 1.666 0.99
186_K 190_D 1.663 0.99
210_D 214_Q 1.659 0.99
386_V 391_G 1.658 0.99
377_P 398_N 1.648 0.99
402_R 405_E 1.637 0.99
387_K 421_T 1.633 0.99
69_I 75_L 1.598 0.98
188_L 192_M 1.594 0.98
80_L 84_M 1.573 0.98
184_Y 188_L 1.564 0.98
311_L 403_I 1.562 0.98
337_D 382_S 1.559 0.98
387_K 422_E 1.556 0.98
158_F 191_T 1.553 0.98
350_M 356_V 1.552 0.98
387_K 391_G 1.527 0.98
345_C 371_P 1.524 0.98
401_K 405_E 1.522 0.98
163_K 184_Y 1.521 0.98
104_C 149_V 1.497 0.97
75_L 149_V 1.495 0.97
403_I 411_I 1.495 0.97
294_Q 338_G 1.487 0.97
346_V 355_E 1.479 0.97
338_G 380_S 1.479 0.97
99_L 208_L 1.475 0.97
155_K 192_M 1.469 0.97
347_A 355_E 1.463 0.97
285_D 288_G 1.452 0.97
105_P 146_G 1.449 0.97
48_G 419_D 1.444 0.96
189_D 193_A 1.442 0.96
68_I 205_V 1.428 0.96
227_T 323_N 1.427 0.96
38_H 107_I 1.419 0.96
27_R 428_D 1.419 0.96
210_D 259_Y 1.408 0.96
81_M 197_I 1.395 0.95
355_E 370_E 1.393 0.95
414_I 424_M 1.391 0.95
320_V 325_L 1.388 0.95
355_E 372_I 1.383 0.95
46_I 61_L 1.376 0.95
99_L 203_V 1.37 0.95
75_L 80_L 1.356 0.94
258_R 324_S 1.355 0.94
288_G 308_T 1.355 0.94
9_G 15_E 1.349 0.94
193_A 196_D 1.342 0.94
284_F 287_T 1.324 0.93
213_R 219_V 1.322 0.93
362_A 365_D 1.319 0.93
71_N 233_H 1.318 0.93
139_E 143_A 1.309 0.93
195_A 199_T 1.308 0.93
374_E 406_L 1.308 0.93
400_I 411_I 1.29 0.92
88_E 95_R 1.285 0.92
410_P 427_R 1.276 0.92
159_A 188_L 1.274 0.92
313_T 402_R 1.273 0.92
350_M 365_D 1.264 0.91
221_F 255_L 1.261 0.91
159_A 184_Y 1.257 0.91
416_T 422_E 1.251 0.91
162_L 188_L 1.244 0.90
390_S 397_L 1.244 0.90
267_L 316_V 1.233 0.90
158_F 162_L 1.211 0.89
159_A 185_Q 1.211 0.89
38_H 79_A 1.203 0.88
258_R 326_S 1.202 0.88
403_I 407_T 1.2 0.88
190_D 193_A 1.19 0.88
214_Q 259_Y 1.187 0.87
77_P 198_L 1.182 0.87
147_L 158_F 1.174 0.87
212_A 217_D 1.174 0.87
289_E 305_R 1.17 0.86
386_V 397_L 1.17 0.86
38_H 76_S 1.169 0.86
400_I 414_I 1.165 0.86
153_F 199_T 1.163 0.86
66_T 99_L 1.153 0.85
337_D 381_E 1.153 0.85
66_T 208_L 1.153 0.85
120_K 131_G 1.15 0.85
80_L 98_L 1.14 0.84
6_V 249_V 1.139 0.84
85_K 88_E 1.134 0.84
163_K 167_E 1.133 0.84
210_D 321_Q 1.133 0.84
404_E 426_L 1.127 0.83
398_N 405_E 1.125 0.83
350_M 354_R 1.125 0.83
314_V 347_A 1.122 0.83
78_A 82_K 1.118 0.83
191_T 194_V 1.103 0.82
102_E 361_L 1.103 0.82
291_L 342_V 1.099 0.81
207_D 211_Q 1.096 0.81
117_A 173_L 1.092 0.81
346_V 374_E 1.086 0.80
339_L 342_V 1.086 0.80
101_S 205_V 1.081 0.80
159_A 192_M 1.074 0.79
286_E 289_E 1.065 0.78
401_K 404_E 1.06 0.78
22_D 420_R 1.058 0.78
209_L 219_V 1.057 0.78
227_T 319_A 1.053 0.77
337_D 380_S 1.047 0.77
291_L 331_T 1.044 0.76
412_D 427_R 1.036 0.76
389_R 426_L 1.035 0.75
359_T 366_W 1.034 0.75
171_F 176_Y 1.033 0.75
320_V 409_V 1.033 0.75
189_D 192_M 1.032 0.75
207_D 321_Q 1.026 0.75
264_L 327_G 1.025 0.74
203_V 208_L 1.022 0.74
400_I 404_E 1.02 0.74
113_A 116_N 1.019 0.74
123_G 276_A 1.016 0.74
229_L 273_R 1.015 0.73
266_I 423_T 1.003 0.72
86_E 89_D 1.001 0.72
103_A 361_L 1 0.72
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3hidA11100-0.003Contact Map0.7
4m9dA20.9931100-0.003Contact Map0.774
3ue9A40.99541000.002Contact Map0.741
2v40A10.97451000.017Contact Map0.716
1p9bA10.97921000.02Contact Map0.72
3r7tA10.9631000.023Contact Map0.683
1dj2A20.98151000.023Contact Map0.697
2d7uA10.78011000.203Contact Map0.71
3dfzA20.41218.20.983Contact Map0.181
4gj4A40.277814.40.984Contact Map0.043
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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