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OPENSEQ.org
DNAK - Chaperone protein DnaK
UniProt: P0A6Y8 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10241
Length: 638 (630)
Sequences: 3349
Seq/Len: 5.32
DNAK
Paralog alert: 0.41 [within 20: 0.03] - ratio of genomes with paralogs
Cluster includes: DNAK HSCA HSCC MREB YEGD
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
137_E 164_E 5.543 1.00
224_D 315_R 4.623 1.00
235_R 311_D 4.582 1.00
236_L 300_V 3.99 1.00
354_A 360_E 3.769 1.00
351_K 355_E 3.587 1.00
68_A 230_E 3.396 1.00
193_Y 316_S 3.358 1.00
241_V 253_R 3.335 1.00
398_S 410_T 3.321 1.00
204_I 323_A 3.18 1.00
220_A 323_A 3.163 1.00
439_H 453_S 3.129 1.00
29_N 36_T 3.09 1.00
509_E 513_Q 3.066 1.00
547_R 573_E 3.054 1.00
580_D 583_A 2.964 1.00
437_T 458_N 2.871 1.00
522_N 525_A 2.85 1.00
278_Q 299_K 2.773 1.00
137_E 166_K 2.69 1.00
191_A 202_I 2.672 1.00
240_L 298_I 2.607 1.00
520_E 523_A 2.59 1.00
410_T 413_A 2.453 1.00
441_L 453_S 2.421 1.00
243_E 304_K 2.418 1.00
366_N 369_E 2.418 1.00
4_I 166_K 2.374 1.00
5_I 16_V 2.344 1.00
343_Q 346_M 2.33 1.00
539_G 572_L 2.304 1.00
527_R 531_E 2.301 1.00
317_I 356_F 2.294 1.00
104_E 107_G 2.284 1.00
563_T 567_S 2.265 1.00
350_Q 363_K 2.265 1.00
304_K 307_S 2.264 1.00
309_V 346_M 2.229 1.00
104_E 109_K 2.189 1.00
232_F 312_L 2.178 1.00
191_A 204_I 2.135 1.00
305_L 343_Q 2.126 1.00
48_T 118_E 2.118 1.00
313_V 348_M 2.117 1.00
33_D 52_Q 2.113 1.00
195_L 232_F 2.077 1.00
124_K 128_E 2.067 1.00
392_L 417_T 2.063 1.00
92_K 104_E 2.045 1.00
570_T 574_T 2.044 1.00
546_T 595_S 2.037 1.00
240_L 266_A 2.035 1.00
400_G 408_M 2.03 1.00
125_K 129_D 2.026 1.00
524_E 528_K 2.011 1.00
571_A 587_K 2.007 1.00
280_D 297_N 1.998 1.00
409_T 426_F 1.98 1.00
237_I 263_K 1.976 1.00
561_D 601_I 1.968 1.00
574_T 577_K 1.961 1.00
69_I 103_V 1.948 1.00
318_E 322_V 1.942 1.00
566_E 570_T 1.931 1.00
600_E 604_Q 1.929 1.00
234_S 238_N 1.924 1.00
559_A 563_T 1.918 1.00
352_K 355_E 1.904 1.00
63_Q 256_P 1.901 1.00
305_L 346_M 1.892 1.00
243_E 246_K 1.89 1.00
489_K 496_E 1.886 1.00
514_K 518_D 1.868 1.00
581_K 585_E 1.859 1.00
597_K 600_E 1.857 1.00
159_R 509_E 1.848 1.00
517_R 520_E 1.83 1.00
587_K 590_E 1.821 1.00
554_G 562_K 1.788 1.00
517_R 521_A 1.783 1.00
330_S 333_D 1.777 1.00
513_Q 517_R 1.775 1.00
15_C 26_V 1.757 1.00
236_L 266_A 1.726 1.00
123_M 126_T 1.711 1.00
518_D 521_A 1.707 1.00
309_V 313_V 1.702 1.00
17_A 376_A 1.699 1.00
252_L 286_I 1.695 1.00
558_P 561_D 1.691 1.00
78_Q 95_A 1.673 1.00
447_R 533_V 1.651 0.99
439_H 456_Q 1.64 0.99
395_T 478_I 1.64 0.99
589_Q 593_Q 1.64 0.99
63_Q 92_K 1.638 0.99
410_T 446_K 1.625 0.99
152_Q 156_D 1.606 0.99
15_C 35_T 1.601 0.99
219_L 329_L 1.601 0.99
585_E 589_Q 1.594 0.99
318_E 321_K 1.579 0.99
159_R 164_E 1.578 0.99
27_L 130_Y 1.576 0.99
57_Q 61_N 1.576 0.99
114_Q 118_E 1.576 0.99
543_L 573_E 1.574 0.99
471_Q 491_K 1.569 0.99
543_L 572_L 1.568 0.99
583_A 587_K 1.567 0.99
447_R 450_D 1.567 0.99
141_T 375_A 1.546 0.99
408_M 441_L 1.527 0.99
69_I 116_S 1.523 0.99
310_E 314_N 1.522 0.99
306_E 351_K 1.518 0.99
437_T 456_Q 1.514 0.99
239_Y 300_V 1.512 0.99
553_A 602_A 1.512 0.99
42_Y 118_E 1.512 0.99
317_I 321_K 1.509 0.99
20_D 25_R 1.502 0.99
289_D 294_K 1.475 0.98
313_V 352_K 1.474 0.98
242_E 253_R 1.474 0.98
304_K 308_L 1.469 0.98
336_D 362_R 1.461 0.98
595_S 599_M 1.458 0.98
67_F 90_P 1.449 0.98
567_S 571_A 1.446 0.98
78_Q 99_G 1.437 0.98
64_N 106_K 1.422 0.98
568_A 590_E 1.422 0.98
321_K 325_Q 1.417 0.98
322_V 414_K 1.413 0.98
110_M 115_I 1.409 0.98
188_R 336_D 1.392 0.98
309_V 348_M 1.386 0.98
204_I 334_I 1.385 0.97
5_I 135_V 1.379 0.97
592_A 596_Q 1.377 0.97
535_T 585_E 1.368 0.97
538_Q 588_M 1.366 0.97
41_A 57_Q 1.36 0.97
26_V 373_I 1.359 0.97
189_T 329_L 1.352 0.97
322_V 325_Q 1.35 0.97
122_K 126_T 1.349 0.97
441_L 448_A 1.345 0.97
596_Q 599_M 1.34 0.97
83_Q 86_V 1.34 0.97
321_K 356_F 1.338 0.97
353_V 361_P 1.331 0.97
156_D 159_R 1.325 0.96
324_L 329_L 1.322 0.96
586_A 590_E 1.317 0.96
403_T 409_T 1.314 0.96
535_T 588_M 1.31 0.96
235_R 309_V 1.308 0.96
4_I 379_G 1.308 0.96
248_Q 294_K 1.307 0.96
240_L 262_L 1.306 0.96
189_T 219_L 1.298 0.96
222_N 322_V 1.294 0.96
245_K 251_D 1.29 0.96
568_A 571_A 1.288 0.96
116_S 154_T 1.288 0.96
289_D 292_G 1.287 0.96
463_N 492_N 1.287 0.96
42_Y 110_M 1.285 0.96
523_A 527_R 1.282 0.96
307_S 310_E 1.281 0.96
195_L 200_F 1.281 0.96
351_K 354_A 1.276 0.95
118_E 121_K 1.266 0.95
2_G 137_E 1.261 0.95
524_E 527_R 1.258 0.95
30_A 126_T 1.25 0.95
423_S 473_E 1.249 0.95
17_A 372_A 1.248 0.95
28_E 130_Y 1.246 0.95
9_L 120_L 1.245 0.95
546_T 569_L 1.236 0.94
265_A 269_A 1.235 0.94
568_A 594_V 1.228 0.94
239_Y 304_K 1.228 0.94
317_I 352_K 1.217 0.94
322_V 326_D 1.216 0.94
402_E 406_G 1.213 0.93
390_L 480_A 1.212 0.93
626_V 629_A 1.209 0.93
453_S 456_Q 1.205 0.93
423_S 475_T 1.198 0.93
267_E 271_I 1.198 0.93
628_D 631_F 1.197 0.93
236_L 239_Y 1.194 0.93
571_A 590_E 1.193 0.93
24_P 373_I 1.193 0.93
404_M 429_A 1.193 0.93
16_V 135_V 1.19 0.93
578_G 583_A 1.19 0.93
113_P 156_D 1.185 0.92
362_R 370_A 1.184 0.92
77_F 83_Q 1.183 0.92
324_L 331_V 1.182 0.92
301_T 304_K 1.179 0.92
560_D 563_T 1.179 0.92
240_L 300_V 1.177 0.92
302_R 306_E 1.176 0.92
562_K 566_E 1.167 0.92
329_L 334_I 1.166 0.92
191_A 320_L 1.165 0.92
492_N 603_Q 1.164 0.92
77_F 86_V 1.164 0.92
536_R 576_L 1.164 0.92
69_I 119_V 1.163 0.91
543_L 569_L 1.162 0.91
34_R 369_E 1.158 0.91
140_I 165_V 1.158 0.91
601_I 604_Q 1.156 0.91
446_K 530_E 1.153 0.91
240_L 281_V 1.152 0.91
6_G 10_G 1.143 0.91
71_R 231_D 1.142 0.90
575_A 587_K 1.139 0.90
261_R 265_A 1.134 0.90
348_M 352_K 1.133 0.90
239_Y 298_I 1.131 0.90
560_D 564_A 1.129 0.90
195_L 312_L 1.123 0.89
19_M 380_G 1.12 0.89
345_R 368_D 1.115 0.89
66_L 105_V 1.115 0.89
561_D 564_A 1.115 0.89
269_A 279_T 1.113 0.89
300_V 305_L 1.111 0.89
530_E 534_Q 1.11 0.89
245_K 253_R 1.103 0.88
140_I 158_G 1.095 0.88
232_F 313_V 1.093 0.88
508_N 511_E 1.092 0.88
8_D 11_T 1.092 0.88
575_A 584_I 1.089 0.87
582_A 585_E 1.086 0.87
61_N 64_N 1.085 0.87
531_E 581_K 1.08 0.87
559_A 562_K 1.078 0.87
66_L 115_I 1.076 0.87
42_Y 115_I 1.076 0.87
191_A 357_F 1.073 0.86
83_Q 87_S 1.073 0.86
158_G 168_I 1.073 0.86
572_L 587_K 1.073 0.86
281_V 300_V 1.072 0.86
181_L 338_I 1.071 0.86
232_F 346_M 1.07 0.86
205_I 218_V 1.068 0.86
435_A 458_N 1.067 0.86
238_N 253_R 1.063 0.86
348_M 351_K 1.061 0.86
354_A 361_P 1.057 0.85
553_A 557_L 1.056 0.85
34_R 366_N 1.05 0.85
126_T 129_D 1.049 0.85
382_L 480_A 1.049 0.85
380_G 386_V 1.047 0.85
288_A 292_G 1.045 0.84
549_Q 553_A 1.045 0.84
465_A 490_D 1.041 0.84
546_T 565_I 1.041 0.84
573_E 577_K 1.037 0.84
204_I 329_L 1.037 0.84
41_A 49_L 1.037 0.84
124_K 163_L 1.034 0.84
409_T 424_Q 1.03 0.83
398_S 444_E 1.03 0.83
159_R 516_V 1.028 0.83
324_L 334_I 1.027 0.83
559_A 566_E 1.026 0.83
539_G 584_I 1.025 0.83
48_T 121_K 1.024 0.83
265_A 279_T 1.024 0.83
445_R 526_D 1.023 0.83
111_A 119_V 1.022 0.83
320_L 356_F 1.021 0.82
532_L 576_L 1.019 0.82
565_I 594_V 1.013 0.82
41_A 61_N 1.012 0.82
551_E 554_G 1.011 0.82
19_M 24_P 1.007 0.81
42_Y 105_V 1.004 0.81
536_R 579_E 1.003 0.81
572_L 584_I 1.001 0.81
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4jneA20.9531000.209Contact Map0.794
3d2fA20.98281000.214Contact Map0.684
4fl9A10.8481000.29Contact Map0.749
1yuwA10.8481000.29Contact Map0.732
2v7yA10.79781000.338Contact Map0.719
3qfuA10.58461000.537Contact Map0.72
3i33A10.58781000.538Contact Map0.74
4j8fA10.8371000.551Contact Map0.739
4gniA20.59251000.563Contact Map0.685
1jceA10.51721000.714Contact Map0.506
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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