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OPENSEQ.org
EFG - Elongation factor G
UniProt: P0A6M8 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10360
Length: 704 (693)
Sequences: 3281
Seq/Len: 4.73
EFG
Paralog alert: 0.37 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: EFG LEPA RF3 TYPA
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
377_V 383_A 5.166 1.00
554_D 558_Q 4.692 1.00
534_Y 554_D 4.339 1.00
140_F 269_A 4.081 1.00
500_D 521_D 3.664 1.00
236_K 247_E 3.625 1.00
310_H 315_E 3.599 1.00
50_M 68_T 3.597 1.00
502_E 517_H 3.567 1.00
496_Q 609_K 3.552 1.00
169_L 265_T 3.494 1.00
156_N 160_T 3.434 1.00
188_M 215_A 3.33 1.00
265_T 281_A 3.288 1.00
116_V 146_R 3.225 1.00
523_Y 577_R 3.168 1.00
533_G 573_D 3.165 1.00
522_M 576_I 3.164 1.00
523_Y 575_G 3.036 1.00
113_Y 123_S 3.03 1.00
336_F 385_A 2.992 1.00
495_R 689_E 2.956 1.00
244_T 247_E 2.918 1.00
212_V 216_N 2.905 1.00
623_T 678_A 2.893 1.00
249_K 284_D 2.878 1.00
114_C 117_G 2.844 1.00
520_I 600_A 2.844 1.00
344_N 376_E 2.794 1.00
209_A 212_V 2.765 1.00
214_L 217_E 2.749 1.00
187_K 245_E 2.747 1.00
539_D 577_R 2.677 1.00
434_A 473_M 2.675 1.00
521_D 579_H 2.662 1.00
534_Y 558_Q 2.629 1.00
528_G 573_D 2.596 1.00
618_K 683_E 2.529 1.00
571_V 605_F 2.516 1.00
635_L 640_G 2.505 1.00
497_K 523_Y 2.486 1.00
217_E 221_N 2.476 1.00
696_Q 700_E 2.435 1.00
152_L 214_L 2.42 1.00
445_F 458_I 2.414 1.00
603_E 607_K 2.32 1.00
537_I 575_G 2.295 1.00
621_V 635_L 2.288 1.00
43_V 48_A 2.255 1.00
321_A 334_T 2.249 1.00
171_L 211_M 2.24 1.00
16_S 111_M 2.176 1.00
218_W 221_N 2.165 1.00
140_F 267_G 2.146 1.00
332_N 440_K 2.145 1.00
634_D 666_Y 2.134 1.00
417_S 459_A 2.131 1.00
214_L 218_W 2.113 1.00
559_E 594_K 2.084 1.00
692_S 696_Q 2.077 1.00
152_L 171_L 2.076 1.00
27_T 86_I 2.067 1.00
183_V 211_M 2.055 1.00
235_E 239_G 2.023 1.00
417_S 457_I 2.02 1.00
423_K 480_E 2.006 1.00
535_E 575_G 1.994 1.00
618_K 655_H 1.993 1.00
553_V 578_L 1.97 1.00
500_D 579_H 1.955 1.00
517_H 582_S 1.949 1.00
519_V 579_H 1.949 1.00
232_E 235_E 1.944 1.00
338_V 377_V 1.929 1.00
223_I 248_I 1.92 1.00
521_D 539_D 1.891 1.00
641_M 688_D 1.886 1.00
153_K 157_Q 1.881 1.00
159_K 221_N 1.872 1.00
192_N 205_E 1.867 1.00
521_D 577_R 1.867 1.00
590_E 594_K 1.866 1.00
312_S 315_E 1.865 1.00
188_M 216_N 1.86 1.00
350_L 403_P 1.85 1.00
356_A 390_D 1.844 1.00
647_S 652_V 1.842 1.00
690_A 698_V 1.827 1.00
345_S 375_K 1.825 1.00
139_A 158_I 1.81 1.00
413_E 446_R 1.806 1.00
186_V 285_Y 1.793 1.00
657_E 686_K 1.788 1.00
68_T 367_H 1.767 1.00
448_W 459_A 1.754 1.00
557_I 597_A 1.754 1.00
343_V 360_F 1.744 1.00
684_F 687_Y 1.74 1.00
13_I 84_I 1.74 1.00
189_K 204_Y 1.725 1.00
557_I 576_I 1.721 1.00
114_C 154_V 1.719 0.99
474_K 480_E 1.715 0.99
317_F 349_V 1.709 0.99
243_L 251_A 1.704 0.99
423_K 482_N 1.702 0.99
697_A 701_A 1.699 0.99
497_K 521_D 1.696 0.99
344_N 347_D 1.695 0.99
421_E 482_N 1.691 0.99
520_I 557_I 1.681 0.99
342_V 376_E 1.657 0.99
618_K 685_L 1.651 0.99
552_A 590_E 1.642 0.99
152_L 218_W 1.638 0.99
352_S 398_C 1.637 0.99
230_S 254_Q 1.636 0.99
518_V 545_I 1.622 0.99
589_S 592_A 1.62 0.99
338_V 383_A 1.601 0.99
154_V 157_Q 1.6 0.99
503_G 506_A 1.593 0.99
126_V 129_Q 1.592 0.99
194_N 197_D 1.591 0.99
169_L 281_A 1.587 0.99
171_L 215_A 1.584 0.99
190_A 208_P 1.579 0.99
695_A 699_I 1.577 0.99
189_K 206_D 1.574 0.99
113_Y 158_I 1.574 0.99
528_G 533_G 1.57 0.99
540_I 544_V 1.565 0.99
167_V 261_I 1.555 0.99
115_A 144_M 1.55 0.99
495_R 609_K 1.547 0.99
490_Y 661_S 1.544 0.99
566_L 699_I 1.544 0.99
336_F 383_A 1.542 0.99
191_I 277_A 1.516 0.98
571_V 601_F 1.515 0.98
187_K 204_Y 1.509 0.98
124_E 677_R 1.507 0.98
206_D 209_A 1.505 0.98
611_V 689_E 1.503 0.98
631_V 674_T 1.494 0.98
545_I 578_L 1.485 0.98
363_I 385_A 1.466 0.98
12_N 85_N 1.462 0.98
48_A 57_Q 1.46 0.98
612_L 698_V 1.453 0.98
532_K 535_E 1.442 0.98
445_F 469_I 1.438 0.98
620_E 653_K 1.437 0.98
123_S 139_A 1.431 0.98
296_N 308_E 1.431 0.98
226_A 255_R 1.418 0.97
250_G 254_Q 1.414 0.97
500_D 519_V 1.405 0.97
471_D 475_R 1.405 0.97
173_I 211_M 1.403 0.97
645_Q 652_V 1.398 0.97
525_L 532_K 1.389 0.97
657_E 685_L 1.389 0.97
450_D 453_S 1.388 0.97
527_P 573_D 1.386 0.97
690_A 694_V 1.383 0.97
643_K 657_E 1.373 0.97
537_I 577_R 1.373 0.97
254_Q 258_N 1.372 0.97
452_E 491_R 1.369 0.97
216_N 220_Q 1.367 0.96
114_C 157_Q 1.363 0.96
34_T 70_A 1.362 0.96
399_D 402_A 1.359 0.96
619_V 663_M 1.358 0.96
209_A 213_E 1.357 0.96
9_R 82_H 1.354 0.96
669_Q 673_L 1.349 0.96
538_N 541_K 1.344 0.96
635_L 656_A 1.343 0.96
439_A 445_F 1.327 0.96
601_F 605_F 1.326 0.96
388_L 391_V 1.326 0.96
342_V 378_R 1.325 0.96
646_E 655_H 1.311 0.95
141_V 154_V 1.309 0.95
518_V 578_L 1.307 0.95
97_I 444_S 1.306 0.95
349_V 360_F 1.304 0.95
319_A 336_F 1.304 0.95
504_K 515_Y 1.303 0.95
620_E 655_H 1.299 0.95
325_A 335_F 1.298 0.95
138_I 286_L 1.298 0.95
522_M 604_G 1.297 0.95
185_L 188_M 1.294 0.95
463_E 665_G 1.292 0.95
628_T 654_I 1.287 0.95
299_L 302_G 1.286 0.95
207_I 215_A 1.278 0.94
171_L 218_W 1.277 0.94
619_V 656_A 1.275 0.94
534_Y 561_L 1.262 0.94
55_Q 471_D 1.26 0.94
603_E 606_K 1.255 0.94
561_L 574_M 1.252 0.93
120_Q 622_E 1.252 0.93
155_V 168_P 1.25 0.93
226_A 229_A 1.249 0.93
27_T 50_M 1.243 0.93
188_M 207_I 1.241 0.93
495_R 611_V 1.237 0.93
470_V 483_V 1.235 0.93
539_D 579_H 1.235 0.93
16_S 103_M 1.233 0.93
525_L 535_E 1.225 0.92
377_V 385_A 1.222 0.92
354_K 392_T 1.22 0.92
173_I 183_V 1.22 0.92
156_N 159_K 1.211 0.92
439_A 458_I 1.211 0.92
231_E 235_E 1.21 0.92
252_L 261_I 1.206 0.92
249_K 285_Y 1.206 0.92
584_H 587_D 1.206 0.92
115_A 141_V 1.205 0.92
213_E 217_E 1.2 0.91
520_I 604_G 1.2 0.91
503_G 507_K 1.193 0.91
111_M 123_S 1.191 0.91
17_A 20_D 1.191 0.91
525_L 575_G 1.19 0.91
114_C 146_R 1.184 0.91
211_M 215_A 1.183 0.91
37_N 43_V 1.181 0.91
431_M 473_M 1.181 0.91
14_G 106_L 1.179 0.90
113_Y 139_A 1.178 0.90
193_W 202_F 1.175 0.90
309_R 404_I 1.171 0.90
236_K 241_E 1.168 0.90
494_I 604_G 1.163 0.90
243_L 248_I 1.163 0.90
502_E 519_V 1.162 0.90
635_L 658_V 1.162 0.90
365_Q 374_I 1.158 0.89
336_F 343_V 1.157 0.89
498_V 608_A 1.153 0.89
15_I 27_T 1.149 0.89
171_L 214_L 1.146 0.89
458_I 469_I 1.143 0.89
37_N 49_T 1.141 0.88
364_V 386_I 1.139 0.88
16_S 109_A 1.139 0.88
628_T 645_Q 1.136 0.88
65_S 87_I 1.135 0.88
6_P 9_R 1.134 0.88
336_F 377_V 1.133 0.88
52_W 64_T 1.132 0.88
236_K 244_T 1.128 0.88
313_D 342_V 1.127 0.88
213_E 216_N 1.122 0.87
186_V 248_I 1.121 0.87
26_T 269_A 1.12 0.87
219_H 223_I 1.119 0.87
13_I 282_V 1.116 0.87
180_T 210_D 1.111 0.87
222_L 226_A 1.109 0.87
567_A 699_I 1.108 0.86
315_E 340_S 1.106 0.86
117_G 157_Q 1.105 0.86
26_T 112_V 1.1 0.86
622_E 653_K 1.099 0.86
100_E 129_Q 1.098 0.86
317_F 338_V 1.098 0.86
245_E 249_K 1.097 0.86
111_M 126_V 1.097 0.86
374_I 377_V 1.094 0.86
504_K 517_H 1.094 0.86
558_Q 562_K 1.09 0.85
503_G 599_I 1.088 0.85
252_L 285_Y 1.087 0.85
10_Y 83_R 1.086 0.85
519_V 580_F 1.085 0.85
299_L 307_A 1.081 0.85
388_L 397_L 1.079 0.84
631_V 670_L 1.075 0.84
663_M 682_M 1.072 0.84
326_T 437_R 1.071 0.84
226_A 233_L 1.068 0.84
318_S 340_S 1.068 0.84
72_W 280_D 1.067 0.84
50_M 66_A 1.065 0.83
639_R 662_E 1.063 0.83
231_E 260_E 1.061 0.83
353_V 395_D 1.057 0.83
33_Y 276_Q 1.055 0.83
632_I 636_S 1.053 0.82
294_A 310_H 1.052 0.82
538_N 547_G 1.049 0.82
508_Q 513_G 1.04 0.81
17_A 21_A 1.037 0.81
561_L 601_F 1.036 0.81
299_L 305_T 1.036 0.81
70_A 86_I 1.034 0.81
71_F 83_R 1.034 0.81
534_Y 557_I 1.033 0.81
526_E 533_G 1.03 0.80
350_L 355_A 1.03 0.80
472_R 476_E 1.025 0.80
448_W 457_I 1.022 0.80
155_V 264_V 1.022 0.80
625_E 652_V 1.021 0.80
194_N 203_E 1.018 0.79
503_G 516_G 1.017 0.79
352_S 405_I 1.017 0.79
498_V 501_V 1.017 0.79
227_A 243_L 1.015 0.79
165_N 225_S 1.015 0.79
186_V 245_E 1.013 0.79
617_M 660_L 1.012 0.79
158_I 264_V 1.007 0.78
152_L 211_M 1.007 0.78
50_M 62_T 1.005 0.78
332_N 444_S 1.004 0.78
173_I 190_A 1.001 0.78
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4kiyV10.99861000.171Contact Map0.748
4fn5A10.99151000.179Contact Map0.741
1darA10.97591000.183Contact Map0.739
2xexA20.98151000.186Contact Map0.779
2dy1A10.93751000.235Contact Map0.704
1n0uA10.97021000.269Contact Map0.605
3j3az10.96451000.276Contact Map0.527
3j25A10.88781000.287Contact Map0.102
2yweA10.68751000.516Contact Map0.712
3cb4D60.68041000.522Contact Map0.676
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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