May 4, 2021 - We are working on upgrading the webserver, some pages may not work.
OPENSEQ.org
GRPE - Protein GrpE
UniProt: P09372 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10416
Length: 197 (177)
Sequences: 1085
Seq/Len: 6.13
GRPE
Paralog alert: 0.02 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
44_V 47_L 3.946 1.00
43_K 48_E 3.638 1.00
94_E 137_F 3.259 1.00
101_S 104_R 2.817 1.00
51_L 58_E 2.693 1.00
60_D 64_R 2.531 1.00
155_Q 189_M 2.418 1.00
135_R 140_E 2.326 1.00
101_S 129_S 2.211 1.00
69_M 72_L 2.12 1.00
132_D 135_R 1.966 1.00
160_V 171_L 1.918 1.00
161_E 194_K 1.911 1.00
94_E 136_K 1.875 1.00
105_A 123_I 1.805 1.00
141_V 176_K 1.794 1.00
47_L 51_L 1.794 1.00
92_I 185_I 1.752 1.00
158_A 191_T 1.744 1.00
92_I 139_V 1.738 1.00
45_A 48_E 1.685 1.00
134_V 139_V 1.671 1.00
72_L 76_T 1.621 1.00
103_D 127_L 1.621 1.00
103_D 176_K 1.599 0.99
140_E 179_T 1.589 0.99
162_S 165_V 1.571 0.99
175_Q 189_M 1.537 0.99
88_L 91_F 1.526 0.99
143_A 147_V 1.508 0.99
38_D 41_D 1.495 0.99
80_I 84_H 1.487 0.99
46_N 50_Q 1.478 0.99
78_L 82_K 1.437 0.99
172_G 191_T 1.413 0.98
70_E 73_R 1.408 0.98
114_P 117_S 1.402 0.98
98_V 133_V 1.398 0.98
132_D 136_K 1.389 0.98
43_K 46_N 1.384 0.98
166_A 169_N 1.359 0.98
42_E 46_N 1.356 0.98
44_V 48_E 1.355 0.98
160_V 191_T 1.345 0.98
59_R 63_L 1.331 0.97
144_E 173_I 1.33 0.97
102_L 127_L 1.323 0.97
167_P 195_A 1.315 0.97
50_Q 53_E 1.287 0.97
92_I 180_L 1.268 0.96
67_A 71_N 1.256 0.96
165_V 171_L 1.245 0.96
150_D 153_V 1.236 0.96
89_E 180_L 1.235 0.96
99_I 134_V 1.212 0.95
144_E 147_V 1.21 0.95
144_E 170_V 1.207 0.95
86_F 183_R 1.197 0.94
106_L 120_V 1.195 0.94
74_R 78_L 1.183 0.94
50_Q 59_R 1.164 0.93
40_R 43_K 1.163 0.93
49_A 53_E 1.16 0.93
131_L 141_V 1.16 0.93
171_L 193_A 1.147 0.93
124_E 128_K 1.133 0.92
81_E 85_K 1.132 0.92
60_D 68_E 1.131 0.92
19_M 22_H 1.129 0.92
65_V 69_M 1.127 0.92
66_K 70_E 1.123 0.92
180_L 185_I 1.123 0.92
159_M 168_G 1.103 0.91
80_I 83_A 1.101 0.91
86_F 181_N 1.084 0.90
65_V 72_L 1.081 0.89
157_I 175_Q 1.074 0.89
153_V 179_T 1.073 0.89
42_E 45_A 1.071 0.89
76_T 80_I 1.049 0.87
96_L 186_R 1.033 0.86
44_V 51_L 1.031 0.86
81_E 84_H 1.016 0.85
85_K 181_N 1.004 0.84
33_S 36_Q 1.003 0.84
144_E 169_N 1.001 0.84
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1dkgA211000.078Contact Map0.747
4aniA40.95941000.145Contact Map0.718
3a6mA20.8681000.161Contact Map0.724
3swyA30.233542.70.937Contact Map0.685
3swfA30.375623.10.945Contact Map0.812
2kjwA10.228411.10.952Contact Map0
4kgdA20.40618.90.954Contact Map0.243
2j5aA10.48738.60.955Contact Map0.175
2w72C10.675180.955Contact Map0.258
4jzpA20.34527.90.955Contact Map0.611
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
Page generated in 0.0385 seconds.