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OPENSEQ.org
LEU1 - 2-isopropylmalate synthase
UniProt: P09151 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11226
Length: 523 (498)
Sequences: 2052
Seq/Len: 4.12
LEU1
Paralog alert: 0.27 [within 20: 0.04] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
48_P 72_A 4.22 1.00
181_A 220_A 3.385 1.00
114_T 117_E 3.231 1.00
120_E 124_Y 3.205 1.00
302_D 306_K 3.165 1.00
82_D 128_R 3.12 1.00
201_V 217_A 3.112 1.00
185_S 189_E 3.055 1.00
188_Y 199_I 3.052 1.00
155_V 166_I 2.925 1.00
122_A 154_V 2.83 1.00
104_P 147_P 2.787 1.00
449_Y 488_S 2.755 1.00
315_T 318_S 2.654 1.00
104_P 115_L 2.643 1.00
38_M 269_T 2.492 1.00
115_L 153_R 2.468 1.00
123_I 127_K 2.425 1.00
119_I 154_V 2.417 1.00
78_E 82_D 2.41 1.00
178_F 219_H 2.385 1.00
123_I 161_A 2.351 1.00
208_G 243_E 2.343 1.00
203_T 214_S 2.33 1.00
107_I 115_L 2.325 1.00
116_D 153_R 2.272 1.00
481_A 490_K 2.244 1.00
122_A 158_A 2.242 1.00
150_D 154_V 2.232 1.00
416_E 419_K 2.214 1.00
156_E 192_P 2.213 1.00
78_E 124_Y 2.197 1.00
265_E 268_R 2.191 1.00
123_I 157_A 2.189 1.00
59_Q 63_R 2.185 1.00
52_P 56_E 2.18 1.00
148_I 186_G 2.178 1.00
421_E 438_R 2.164 1.00
116_D 119_I 2.159 1.00
445_E 469_N 2.153 1.00
130_R 164_T 2.096 1.00
110_K 141_E 2.084 1.00
432_V 489_A 2.078 1.00
168_I 187_L 2.052 1.00
36_E 67_N 2.016 1.00
113_S 121_R 2.008 1.00
186_G 190_R 1.992 1.00
481_A 486_E 1.975 1.00
262_N 265_E 1.973 1.00
119_I 153_R 1.958 1.00
34_A 37_R 1.952 1.00
239_C 244_V 1.938 1.00
78_E 128_R 1.933 1.00
126_V 136_V 1.921 1.00
55_F 83_V 1.911 1.00
464_V 487_S 1.902 1.00
341_M 381_I 1.896 1.00
102_T 146_T 1.865 1.00
199_I 222_A 1.839 1.00
66_K 92_E 1.795 1.00
7_I 40_V 1.789 1.00
214_S 225_V 1.779 0.99
149_A 152_A 1.743 0.99
270_S 281_I 1.729 0.99
181_A 185_S 1.726 0.99
36_E 68_S 1.723 0.99
82_D 132_Y 1.719 0.99
30_Q 275_Q 1.718 0.99
411_K 418_V 1.711 0.99
185_S 220_A 1.704 0.99
138_F 154_V 1.668 0.99
304_V 324_I 1.666 0.99
447_V 469_N 1.64 0.99
115_L 150_D 1.631 0.99
140_C 155_V 1.628 0.99
60_T 64_Q 1.616 0.99
394_D 418_V 1.612 0.99
88_L 94_F 1.608 0.99
107_I 146_T 1.605 0.99
40_V 261_I 1.602 0.99
117_E 120_E 1.599 0.99
6_I 42_V 1.591 0.99
101_A 106_H 1.576 0.98
107_I 147_P 1.556 0.98
194_I 199_I 1.552 0.98
168_I 183_I 1.552 0.98
245_I 261_I 1.548 0.98
123_I 158_A 1.532 0.98
107_I 150_D 1.529 0.98
211_V 239_C 1.52 0.98
390_H 414_C 1.512 0.98
181_A 219_H 1.503 0.98
59_Q 90_V 1.503 0.98
182_G 186_G 1.5 0.98
115_L 147_P 1.499 0.98
180_F 201_V 1.497 0.98
170_D 213_N 1.479 0.97
432_V 485_V 1.473 0.97
34_A 272_L 1.47 0.97
53_G 365_D 1.465 0.97
152_A 187_L 1.464 0.97
411_K 420_A 1.463 0.97
32_A 57_S 1.458 0.97
31_I 273_V 1.458 0.97
23_L 31_I 1.454 0.97
123_I 160_N 1.444 0.97
52_P 83_V 1.444 0.97
152_A 156_E 1.442 0.97
119_I 150_D 1.44 0.97
34_A 269_T 1.437 0.97
128_R 132_Y 1.429 0.97
4_Q 260_A 1.427 0.97
85_A 133_T 1.426 0.97
193_N 197_A 1.421 0.97
389_E 392_R 1.417 0.96
106_H 142_D 1.413 0.96
113_S 117_E 1.413 0.96
6_I 165_T 1.409 0.96
69_R 93_A 1.399 0.96
101_A 142_D 1.399 0.96
48_P 58_V 1.383 0.96
116_D 120_E 1.374 0.96
337_V 356_L 1.373 0.96
99_F 299_I 1.372 0.96
407_T 422_A 1.366 0.95
101_A 141_E 1.365 0.95
86_E 89_K 1.361 0.95
137_E 200_S 1.36 0.95
230_N 270_S 1.358 0.95
109_T 307_N 1.353 0.95
152_A 186_G 1.35 0.95
122_A 157_A 1.341 0.95
296_S 331_R 1.338 0.95
144_G 175_T 1.327 0.94
145_R 174_Y 1.326 0.94
155_V 168_I 1.324 0.94
214_S 218_V 1.322 0.94
470_Y 499_I 1.319 0.94
63_R 90_V 1.319 0.94
72_A 84_A 1.318 0.94
466_I 492_M 1.315 0.94
469_N 472_G 1.313 0.94
211_V 244_V 1.311 0.94
218_V 225_V 1.307 0.94
101_A 110_K 1.306 0.94
56_E 60_T 1.304 0.94
23_L 276_I 1.303 0.94
184_I 201_V 1.302 0.94
426_N 482_T 1.293 0.93
426_N 430_D 1.284 0.93
409_A 422_A 1.283 0.93
50_S 331_R 1.279 0.93
156_E 190_R 1.279 0.93
76_C 98_T 1.279 0.93
423_A 434_Q 1.278 0.93
180_F 184_I 1.274 0.93
100_I 138_F 1.271 0.92
5_V 257_V 1.265 0.92
214_S 244_V 1.26 0.92
83_V 86_E 1.257 0.92
105_M 109_T 1.256 0.92
3_Q 223_R 1.25 0.92
159_I 166_I 1.245 0.91
157_A 160_N 1.237 0.91
215_L 247_A 1.236 0.91
113_S 118_V 1.236 0.91
10_T 106_H 1.226 0.91
296_S 329_T 1.226 0.91
82_D 86_E 1.224 0.91
85_A 125_M 1.221 0.90
301_Q 336_A 1.219 0.90
128_R 131_N 1.215 0.90
184_I 220_A 1.215 0.90
25_V 60_T 1.209 0.90
468_A 495_V 1.197 0.89
126_V 161_A 1.197 0.89
145_R 179_E 1.193 0.89
23_L 277_C 1.188 0.89
211_V 214_S 1.187 0.89
214_S 227_G 1.182 0.88
339_H 342_D 1.178 0.88
267_W 321_L 1.177 0.88
227_G 245_I 1.172 0.88
107_I 118_V 1.17 0.88
198_I 223_R 1.163 0.87
394_D 411_K 1.159 0.87
187_L 199_I 1.158 0.87
37_R 265_E 1.154 0.87
61_I 70_V 1.154 0.87
111_L 141_E 1.153 0.87
205_D 210_A 1.153 0.87
156_E 160_N 1.152 0.87
359_A 362_K 1.149 0.86
156_E 159_I 1.149 0.86
110_K 302_D 1.142 0.86
104_P 108_A 1.139 0.86
216_A 220_A 1.137 0.86
98_T 125_M 1.133 0.85
49_V 79_K 1.13 0.85
436_I 489_A 1.129 0.85
104_P 107_I 1.121 0.85
53_G 330_S 1.119 0.84
421_E 439_I 1.116 0.84
422_A 438_R 1.114 0.84
205_D 208_G 1.114 0.84
41_D 67_N 1.111 0.84
232_I 279_M 1.111 0.84
45_V 58_V 1.108 0.84
140_C 168_I 1.107 0.84
58_V 72_A 1.101 0.83
214_S 239_C 1.099 0.83
43_M 70_V 1.093 0.82
391_F 412_L 1.09 0.82
166_I 199_I 1.09 0.82
141_E 311_Y 1.089 0.82
142_D 145_R 1.089 0.82
152_A 190_R 1.086 0.82
352_N 355_N 1.083 0.82
395_Y 411_K 1.081 0.81
397_S 401_G 1.076 0.81
166_I 197_A 1.074 0.81
56_E 59_Q 1.071 0.81
272_L 276_I 1.07 0.81
18_A 21_A 1.07 0.81
26_K 60_T 1.066 0.80
37_R 268_R 1.062 0.80
368_G 374_D 1.056 0.79
104_P 150_D 1.053 0.79
357_Y 361_L 1.048 0.79
484_I 488_S 1.041 0.78
156_E 191_V 1.026 0.77
76_C 125_M 1.025 0.76
21_A 31_I 1.021 0.76
19_L 291_G 1.017 0.76
208_G 240_S 1.011 0.75
152_A 192_P 1.006 0.75
346_Y 376_E 1.005 0.74
180_F 217_A 1.005 0.74
335_A 339_H 1.002 0.74
90_V 94_F 1.002 0.74
60_T 63_R 1.002 0.74
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3hq1A20.94261000.248Contact Map0.739
3rmjA20.69021000.42Contact Map0.771
2ztjA10.72471000.426Contact Map0.638
3ivsA20.73231000.447Contact Map0.653
3bleA10.59661000.524Contact Map0.634
3eegA20.61761000.539Contact Map0.704
2qf7A20.90631000.598Contact Map0.474
3ewbX10.55831000.613Contact Map0.699
2cw6A60.53351000.622Contact Map0.63
1ydoA40.55071000.626Contact Map0.509
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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