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OPENSEQ.org
FDHF - Formate dehydrogenase H
UniProt: P07658 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10285
Length: 715 (684)
Sequences: 4669
Seq/Len: 6.83
FDHF
Paralog alert: 0.78 [within 20: 0.14] - ratio of genomes with paralogs
Cluster includes: BISC DMSA FDHF FDNG FDOG NAPA TORA TORZ YDEP YNFE YNFF
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
64_T 77_P 6.119 1.00
620_I 639_A 5.325 1.00
85_N 488_R 4.846 1.00
627_W 686_E 4.221 1.00
476_K 484_E 3.897 1.00
624_A 687_P 3.846 1.00
614_D 648_G 3.723 1.00
229_A 267_V 3.649 1.00
237_E 283_R 3.617 1.00
90_R 93_A 3.535 1.00
265_E 275_A 3.405 1.00
575_M 618_L 3.388 1.00
467_K 470_E 3.281 1.00
84_L 488_R 3.276 1.00
627_W 634_K 3.265 1.00
242_K 246_A 3.233 1.00
610_I 639_A 3.153 1.00
233_V 237_E 3.146 1.00
480_Q 484_E 3.121 1.00
268_E 275_A 3.115 1.00
250_E 552_N 3.061 1.00
427_V 439_A 3.047 1.00
86_Y 441_V 3.034 1.00
232_H 236_E 2.954 1.00
231_G 255_Y 2.899 1.00
168_D 290_S 2.805 1.00
87_V 441_V 2.773 1.00
93_A 97_K 2.768 1.00
78_V 441_V 2.743 1.00
69_R 76_E 2.722 1.00
613_E 647_K 2.702 1.00
94_I 102_A 2.685 1.00
626_V 639_A 2.678 1.00
630_S 683_V 2.675 1.00
67_I 441_V 2.651 1.00
79_S 82_E 2.58 1.00
277_E 280_Q 2.569 1.00
226_L 271_T 2.556 1.00
615_A 641_V 2.548 1.00
231_G 318_L 2.518 1.00
64_T 474_D 2.498 1.00
274_S 277_E 2.488 1.00
238_N 242_K 2.455 1.00
173_F 227_L 2.44 1.00
610_I 685_V 2.434 1.00
629_H 684_R 2.421 1.00
200_V 212_A 2.416 1.00
67_I 78_V 2.414 1.00
91_L 397_A 2.41 1.00
84_L 484_E 2.394 1.00
484_E 488_R 2.358 1.00
275_A 279_R 2.357 1.00
67_I 86_Y 2.33 1.00
240_Y 245_V 2.329 1.00
263_T 266_S 2.327 1.00
629_H 634_K 2.298 1.00
235_I 252_F 2.292 1.00
243_A 247_S 2.291 1.00
622_D 641_V 2.287 1.00
552_N 556_Q 2.278 1.00
214_M 280_Q 2.268 1.00
618_L 684_R 2.265 1.00
617_R 647_K 2.264 1.00
629_H 686_E 2.247 1.00
77_P 474_D 2.244 1.00
620_I 626_V 2.242 1.00
608_A 650_I 2.225 1.00
87_V 426_I 2.205 1.00
197_K 284_M 2.169 1.00
604_E 638_R 2.169 1.00
86_Y 90_R 2.137 1.00
324_K 327_A 2.126 1.00
216_I 281_A 2.117 1.00
232_H 264_P 2.117 1.00
169_L 284_M 2.032 1.00
167_T 170_V 2.031 1.00
216_I 273_V 2.024 1.00
66_M 442_I 2.016 1.00
396_R 423_E 2.014 1.00
422_L 425_V 2.01 1.00
280_Q 284_M 1.979 1.00
233_V 264_P 1.964 1.00
78_V 86_Y 1.96 1.00
399_Y 428_Q 1.955 1.00
497_N 500_E 1.954 1.00
268_E 274_S 1.943 1.00
254_E 257_K 1.916 1.00
516_T 519_K 1.899 1.00
166_N 325_P 1.895 1.00
67_I 76_E 1.889 1.00
391_A 414_A 1.875 1.00
235_I 256_R 1.873 1.00
257_K 260_E 1.868 1.00
622_D 640_Q 1.866 1.00
608_A 626_V 1.864 1.00
85_N 89_E 1.859 1.00
18_N 32_A 1.858 1.00
318_L 557_F 1.854 1.00
432_M 436_A 1.844 1.00
226_L 278_I 1.841 1.00
171_F 201_C 1.838 1.00
625_L 636_I 1.837 1.00
169_L 199_I 1.824 1.00
233_V 283_R 1.812 1.00
235_I 259_V 1.793 1.00
285_Y 319_T 1.772 1.00
214_M 277_E 1.771 1.00
411_E 667_E 1.765 1.00
16_K 34_G 1.761 1.00
472_K 475_L 1.76 1.00
627_W 636_I 1.747 1.00
416_R 420_E 1.73 1.00
637_T 652_M 1.724 1.00
240_Y 252_F 1.713 1.00
239_L 286_A 1.709 1.00
621_E 624_A 1.7 1.00
201_C 226_L 1.694 1.00
625_L 638_R 1.693 1.00
82_E 86_Y 1.691 1.00
191_A 196_A 1.686 1.00
19_L 26_I 1.685 1.00
285_Y 291_A 1.682 1.00
424_L 441_V 1.673 1.00
236_E 260_E 1.667 1.00
249_T 557_F 1.662 1.00
91_L 426_I 1.652 1.00
427_V 436_A 1.65 1.00
233_V 239_L 1.642 1.00
222_S 270_I 1.635 1.00
237_E 279_R 1.633 1.00
580_V 651_Y 1.632 1.00
214_M 284_M 1.631 1.00
496_N 500_E 1.614 1.00
218_L 273_V 1.61 1.00
265_E 279_R 1.605 1.00
239_L 283_R 1.592 1.00
94_I 397_A 1.59 1.00
65_P 80_W 1.587 1.00
225_A 270_I 1.581 1.00
452_E 467_K 1.581 1.00
226_L 281_A 1.579 1.00
267_V 278_I 1.575 1.00
400_I 425_V 1.566 1.00
192_K 213_D 1.559 1.00
608_A 652_M 1.553 0.99
266_S 269_D 1.553 0.99
650_I 683_V 1.553 0.99
608_A 628_V 1.544 0.99
613_E 616_K 1.54 0.99
98_Y 396_R 1.534 0.99
364_E 368_K 1.522 0.99
243_A 246_A 1.517 0.99
575_M 650_I 1.515 0.99
570_T 631_R 1.508 0.99
417_K 420_E 1.508 0.99
299_V 312_L 1.508 0.99
170_V 188_V 1.5 0.99
499_Q 503_D 1.499 0.99
89_E 93_A 1.491 0.99
268_E 278_I 1.481 0.99
11_C 15_C 1.481 0.99
105_T 124_A 1.479 0.99
234_I 240_Y 1.467 0.99
169_L 281_A 1.456 0.99
102_A 396_R 1.453 0.99
92_S 96_E 1.452 0.99
236_E 279_R 1.451 0.99
610_I 650_I 1.451 0.99
63_K 473_W 1.449 0.99
635_I 652_M 1.443 0.99
87_V 443_L 1.442 0.99
268_E 276_S 1.439 0.99
78_V 82_E 1.438 0.99
446_T 477_T 1.436 0.99
233_V 279_R 1.431 0.99
245_V 252_F 1.43 0.99
484_E 487_T 1.428 0.99
171_F 281_A 1.428 0.99
8_C 11_C 1.424 0.99
99_G 102_A 1.414 0.99
283_R 287_Q 1.413 0.99
230_M 285_Y 1.412 0.99
232_H 263_T 1.409 0.99
388_H 414_A 1.407 0.99
147_G 309_V 1.406 0.99
123_F 489_M 1.403 0.99
612_T 643_D 1.402 0.99
251_G 254_E 1.4 0.99
575_M 614_D 1.397 0.99
609_Q 642_S 1.394 0.99
618_L 685_V 1.393 0.99
487_T 492_P 1.39 0.99
124_A 130_T 1.382 0.99
642_S 645_P 1.372 0.98
200_V 209_A 1.371 0.98
575_M 684_R 1.37 0.98
66_M 77_P 1.368 0.98
4_V 21_V 1.366 0.98
84_L 476_K 1.362 0.98
386_L 395_V 1.361 0.98
250_E 556_Q 1.356 0.98
519_K 523_L 1.346 0.98
518_E 522_E 1.333 0.98
245_V 557_F 1.329 0.98
285_Y 293_I 1.324 0.98
611_N 647_K 1.323 0.98
233_V 278_I 1.313 0.98
94_I 423_E 1.312 0.98
82_E 441_V 1.312 0.98
628_V 652_M 1.306 0.98
572_E 631_R 1.303 0.98
163_E 166_N 1.3 0.98
222_S 271_T 1.295 0.97
391_A 417_K 1.286 0.97
288_A 291_A 1.272 0.97
428_Q 481_I 1.272 0.97
203_P 215_H 1.271 0.97
63_K 77_P 1.27 0.97
567_D 632_K 1.267 0.97
432_M 442_I 1.264 0.97
262_Y 267_V 1.26 0.97
230_M 234_I 1.245 0.97
214_M 281_A 1.243 0.97
26_I 52_I 1.239 0.96
86_Y 424_L 1.237 0.96
367_A 374_S 1.232 0.96
8_C 15_C 1.225 0.96
19_L 29_A 1.215 0.96
503_D 506_R 1.21 0.96
267_V 271_T 1.209 0.96
20_V 30_E 1.208 0.96
94_I 424_L 1.205 0.96
297_M 301_Q 1.202 0.96
607_Y 640_Q 1.202 0.96
262_Y 270_I 1.202 0.96
608_A 685_V 1.199 0.96
428_Q 445_S 1.198 0.96
252_F 256_R 1.196 0.95
256_R 260_E 1.195 0.95
227_L 312_L 1.195 0.95
171_F 230_M 1.195 0.95
476_K 480_Q 1.193 0.95
164_I 292_A 1.192 0.95
261_G 266_S 1.191 0.95
69_R 74_K 1.189 0.95
229_A 278_I 1.179 0.95
242_K 256_R 1.176 0.95
430_I 447_S 1.174 0.95
216_I 277_E 1.173 0.95
620_I 641_V 1.17 0.95
397_A 426_I 1.168 0.95
31_A 39_G 1.167 0.95
235_I 240_Y 1.166 0.95
80_W 481_I 1.166 0.95
321_N 327_A 1.164 0.95
446_T 475_L 1.162 0.94
233_V 236_E 1.154 0.94
146_A 672_I 1.153 0.94
575_M 685_V 1.15 0.94
92_S 95_K 1.148 0.94
359_D 362_N 1.147 0.94
50_D 597_A 1.142 0.94
610_I 620_I 1.141 0.94
397_A 424_L 1.141 0.94
608_A 637_T 1.139 0.94
6_T 19_L 1.138 0.94
56_Q 594_N 1.135 0.93
533_T 537_D 1.13 0.93
165_D 187_H 1.124 0.93
234_I 286_A 1.119 0.93
170_V 196_A 1.115 0.93
610_I 615_A 1.114 0.93
477_T 480_Q 1.114 0.93
52_I 469_V 1.114 0.93
168_D 288_A 1.114 0.93
5_V 18_N 1.109 0.92
15_C 42_C 1.108 0.92
232_H 260_E 1.108 0.92
104_Q 386_L 1.105 0.92
172_V 175_Y 1.104 0.92
391_A 421_D 1.103 0.92
262_Y 266_S 1.102 0.92
218_L 222_S 1.096 0.92
255_Y 318_L 1.094 0.92
87_V 424_L 1.094 0.92
170_V 191_A 1.093 0.92
443_L 481_I 1.091 0.92
652_M 661_C 1.089 0.92
227_L 311_S 1.085 0.91
252_F 318_L 1.082 0.91
466_F 521_G 1.081 0.91
24_G 470_E 1.08 0.91
76_E 82_E 1.08 0.91
480_Q 487_T 1.078 0.91
401_M 482_I 1.077 0.91
11_C 42_C 1.073 0.91
84_L 480_Q 1.073 0.91
103_I 397_A 1.069 0.91
25_K 53_N 1.069 0.91
75_L 442_I 1.064 0.90
192_K 198_I 1.061 0.90
192_K 211_I 1.061 0.90
290_S 327_A 1.06 0.90
229_A 233_V 1.058 0.90
84_L 485_I 1.057 0.90
231_G 259_V 1.055 0.90
164_I 188_V 1.055 0.90
68_R 75_L 1.054 0.90
462_F 515_A 1.054 0.90
173_F 201_C 1.053 0.90
611_N 641_V 1.043 0.89
241_D 324_K 1.04 0.89
275_A 278_I 1.039 0.89
609_Q 640_Q 1.038 0.89
388_H 392_H 1.036 0.89
232_H 259_V 1.034 0.88
279_R 283_R 1.032 0.88
268_E 273_V 1.029 0.88
317_M 555_A 1.026 0.88
589_R 593_G 1.024 0.88
239_L 287_Q 1.024 0.88
355_Q 365_K 1.024 0.88
19_L 449_G 1.021 0.88
201_C 281_A 1.016 0.87
8_C 45_G 1.016 0.87
236_E 264_P 1.016 0.87
230_M 282_A 1.016 0.87
163_E 326_H 1.015 0.87
249_T 317_M 1.011 0.87
452_E 464_R 1.01 0.87
104_Q 395_V 1.007 0.87
276_S 279_R 1.006 0.87
169_L 288_A 1.005 0.87
205_K 217_A 1.005 0.87
250_E 554_L 1.003 0.86
230_M 281_A 1.001 0.86
637_T 661_C 1 0.86
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2iv2X111000.103Contact Map0.791
2napA10.94971000.145Contact Map0.784
3ml1A10.95381000.165Contact Map0.761
1h0hA20.9511000.167Contact Map0.724
1kqfA10.94831000.172Contact Map0.749
2e7zA10.91891000.188Contact Map0.775
2vpzA20.95241000.196Contact Map0.744
4aayA40.92871000.211Contact Map0.737
2ivfA10.94271000.213Contact Map0.749
1g8kA40.92451000.221Contact Map0.766
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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