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OPENSEQ.org
BTUE - Vitamin B12 transport periplasmic protein BtuE
UniProt: P06610 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10129
Length: 183 (168)
Sequences: 1985
Seq/Len: 11.82
BTUE
Paralog alert: 0.22 [within 20: 0.02] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
47_E 51_K 3.225 1.00
153_G 157_K 3.068 1.00
52_A 174_M 2.993 1.00
76_D 95_S 2.931 1.00
98_E 103_G 2.696 1.00
162_F 176_S 2.662 1.00
61_L 94_F 2.533 1.00
174_M 178_K 2.427 1.00
159_I 180_A 2.378 1.00
12_D 16_E 2.37 1.00
45_Q 169_E 2.179 1.00
11_K 17_V 2.178 1.00
18_T 23_F 2.165 1.00
26_N 59_M 2.144 1.00
9_V 17_V 2.143 1.00
63_F 94_F 2.097 1.00
100_N 112_I 2.058 1.00
5_I 28_L 2.058 1.00
14_D 80_K 1.964 1.00
97_I 105_H 1.936 1.00
104_R 109_Q 1.932 1.00
21_E 24_A 1.884 1.00
67_Q 98_E 1.814 1.00
43_Y 72_E 1.809 1.00
13_I 76_D 1.808 1.00
50_Q 54_V 1.777 1.00
75_S 78_E 1.715 1.00
39_L 164_P 1.688 1.00
10_V 105_H 1.675 1.00
48_N 169_E 1.657 1.00
160_Q 176_S 1.637 1.00
66_N 70_E 1.567 1.00
8_T 110_K 1.55 1.00
27_V 154_R 1.545 1.00
56_R 178_K 1.54 1.00
48_N 52_A 1.529 1.00
13_I 93_M 1.527 1.00
29_L 151_L 1.48 1.00
29_L 173_V 1.466 1.00
12_D 18_T 1.457 1.00
148_E 161_R 1.449 1.00
5_I 8_T 1.447 1.00
66_N 73_P 1.443 1.00
19_T 22_K 1.434 1.00
50_Q 59_M 1.409 1.00
104_R 112_I 1.403 0.99
44_E 84_T 1.401 0.99
47_E 90_T 1.383 0.99
153_G 180_A 1.372 0.99
18_T 22_K 1.372 0.99
153_G 159_I 1.346 0.99
115_A 158_V 1.324 0.99
8_T 107_L 1.313 0.99
62_G 72_E 1.309 0.99
63_F 97_I 1.307 0.99
33_V 43_Y 1.299 0.99
62_G 93_M 1.293 0.99
9_V 19_T 1.29 0.99
11_K 95_S 1.287 0.99
144_L 148_E 1.286 0.99
62_G 89_V 1.249 0.99
44_E 48_N 1.248 0.99
27_V 180_A 1.245 0.98
40_T 82_Y 1.218 0.98
13_I 80_K 1.2 0.98
143_I 150_F 1.165 0.97
28_L 59_M 1.164 0.97
18_T 94_F 1.157 0.97
150_F 161_R 1.14 0.97
74_G 78_E 1.14 0.97
176_S 179_L 1.136 0.97
45_Q 167_T 1.13 0.97
20_L 107_L 1.128 0.97
79_I 96_K 1.113 0.96
4_S 7_T 1.112 0.96
6_L 24_A 1.094 0.96
97_I 108_Y 1.081 0.95
39_L 165_D 1.072 0.95
53_W 57_G 1.071 0.95
57_G 181_L 1.066 0.95
73_P 96_K 1.06 0.95
72_E 93_M 1.058 0.95
59_M 92_P 1.047 0.94
5_I 30_I 1.044 0.94
81_T 85_T 1.038 0.94
65_C 98_E 1.035 0.94
23_F 94_F 1.021 0.93
51_K 90_T 1.013 0.93
35_S 72_E 1.009 0.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2p5qA40.87981000.608Contact Map0.849
2i3yA10.961799.90.617Contact Map0.766
2r37A20.961799.90.617Contact Map0.787
2f8aA20.983699.90.621Contact Map0.807
3dwvA20.885299.90.643Contact Map0.64
2p31A20.852599.90.646Contact Map0.756
2vupA10.885299.90.648Contact Map0.811
3cmiA10.885299.90.654Contact Map0.779
3kijA30.857999.90.656Contact Map0.758
2v1mA10.879899.90.66Contact Map0.837
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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