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OPENSEQ.org
KEFC - Glutathione-regulated potassium-efflux system protein KefC
UniProt: P03819 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10521
Length: 620 (588)
Sequences: 1424
Seq/Len: 2.42
KEFC
Paralog alert: 0.54 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: KEFB KEFC YBAL
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
467_L 484_V 4.632 1.00
312_W 329_A 2.912 1.00
467_L 491_L 2.833 1.00
469_N 481_T 2.393 1.00
485_K 493_I 2.242 1.00
404_I 415_G 2.241 1.00
484_V 491_L 2.143 1.00
98_G 120_M 2.077 1.00
139_L 144_M 2.077 1.00
300_L 366_L 2.057 1.00
260_A 264_D 2.046 0.99
17_A 225_F 2.002 0.99
14_G 225_F 1.993 0.99
289_L 362_L 1.991 0.99
204_R 256_E 1.955 0.99
485_K 512_E 1.897 0.99
481_T 511_V 1.883 0.99
102_G 119_G 1.871 0.99
304_L 370_A 1.826 0.99
198_L 202_V 1.797 0.98
405_A 467_L 1.794 0.98
527_R 531_E 1.735 0.98
302_G 306_I 1.696 0.97
297_V 301_L 1.688 0.97
315_A 325_R 1.681 0.97
335_G 364_V 1.663 0.97
121_T 343_F 1.66 0.97
300_L 367_S 1.643 0.97
96_I 100_L 1.64 0.97
129_I 368_M 1.638 0.97
94_M 154_F 1.611 0.96
100_L 310_M 1.61 0.96
36_I 40_I 1.608 0.96
457_E 462_A 1.602 0.96
404_I 468_I 1.546 0.95
478_L 502_H 1.536 0.95
334_Q 371_T 1.526 0.94
335_G 339_A 1.521 0.94
401_R 465_E 1.518 0.94
296_I 359_S 1.516 0.94
41_I 48_L 1.507 0.94
203_T 226_L 1.505 0.94
359_S 363_A 1.504 0.94
105_C 115_A 1.503 0.94
67_L 124_L 1.502 0.94
284_I 342_V 1.498 0.94
285_D 358_K 1.495 0.93
461_A 488_F 1.494 0.93
97_C 155_Q 1.488 0.93
120_M 162_L 1.485 0.93
520_E 524_K 1.481 0.93
402_V 418_L 1.48 0.93
428_L 446_F 1.472 0.93
520_E 548_R 1.472 0.93
517_E 521_G 1.465 0.92
117_L 346_A 1.46 0.92
511_V 514_P 1.459 0.92
531_E 540_A 1.459 0.92
125_S 155_Q 1.454 0.92
345_A 348_M 1.447 0.92
103_L 107_L 1.444 0.92
303_F 335_G 1.436 0.91
162_L 342_V 1.428 0.91
166_I 349_A 1.428 0.91
93_Q 338_F 1.419 0.91
212_R 433_D 1.417 0.91
256_E 439_R 1.416 0.91
469_N 477_N 1.413 0.91
29_G 132_Q 1.413 0.91
482_E 509_A 1.403 0.90
98_G 162_L 1.402 0.90
343_F 360_L 1.395 0.90
303_F 363_A 1.383 0.89
402_V 468_I 1.371 0.89
461_A 467_L 1.371 0.89
312_W 322_N 1.366 0.89
500_V 551_N 1.364 0.88
117_L 121_T 1.361 0.88
499_D 502_H 1.361 0.88
284_I 365_A 1.359 0.88
290_L 293_P 1.359 0.88
110_L 118_I 1.353 0.88
518_T 522_A 1.353 0.88
403_I 460_G 1.351 0.88
102_G 116_E 1.349 0.88
15_S 41_I 1.338 0.87
80_W 83_R 1.337 0.87
56_L 279_G 1.334 0.87
197_L 201_Y 1.33 0.87
152_L 338_F 1.319 0.86
147_S 320_V 1.316 0.86
72_L 250_V 1.314 0.86
339_A 364_V 1.304 0.85
403_I 461_A 1.301 0.85
529_A 533_L 1.297 0.85
295_R 298_I 1.293 0.85
121_T 162_L 1.288 0.84
466_V 529_A 1.288 0.84
410_F 525_T 1.28 0.84
128_A 152_L 1.276 0.84
404_I 498_R 1.275 0.83
51_D 54_S 1.271 0.83
461_A 487_H 1.266 0.83
252_L 261_L 1.266 0.83
297_V 306_I 1.265 0.83
279_G 429_D 1.261 0.83
139_L 375_L 1.258 0.82
155_Q 159_A 1.254 0.82
223_A 261_L 1.253 0.82
522_A 529_A 1.251 0.82
468_I 529_A 1.25 0.82
497_A 503_Y 1.25 0.82
353_E 356_W 1.248 0.82
155_Q 310_M 1.247 0.82
564_D 586_D 1.247 0.82
28_L 35_L 1.238 0.81
130_A 195_V 1.237 0.81
128_A 274_G 1.236 0.81
518_T 521_G 1.235 0.81
160_I 242_A 1.234 0.81
415_G 425_M 1.234 0.81
474_P 477_N 1.232 0.80
473_D 478_L 1.229 0.80
473_D 476_T 1.224 0.80
299_L 363_A 1.22 0.80
575_T 583_I 1.22 0.80
264_D 452_R 1.219 0.79
99_G 373_I 1.214 0.79
102_G 112_W 1.21 0.79
262_E 274_G 1.203 0.78
183_L 187_K 1.2 0.78
79_L 87_F 1.197 0.78
144_M 327_W 1.195 0.78
137_R 207_L 1.195 0.78
45_G 49_V 1.191 0.77
73_E 128_A 1.19 0.77
431_D 434_H 1.19 0.77
195_V 247_L 1.184 0.77
461_A 491_L 1.181 0.76
119_G 123_A 1.181 0.76
407_F 415_G 1.179 0.76
316_R 322_N 1.179 0.76
552_I 561_V 1.177 0.76
87_F 91_A 1.176 0.76
245_A 325_R 1.176 0.76
122_L 409_R 1.175 0.76
471_I 480_L 1.172 0.76
123_A 155_Q 1.169 0.75
519_F 522_A 1.167 0.75
72_L 252_L 1.166 0.75
427_V 443_M 1.165 0.75
434_H 499_D 1.163 0.75
99_G 102_G 1.163 0.75
409_R 498_R 1.161 0.75
125_S 128_A 1.16 0.75
64_V 67_L 1.158 0.74
95_V 99_G 1.154 0.74
148_A 280_V 1.154 0.74
63_V 271_L 1.152 0.74
48_L 435_I 1.15 0.74
571_V 575_T 1.15 0.74
409_R 496_R 1.145 0.73
516_R 519_F 1.144 0.73
223_A 278_I 1.141 0.73
225_F 268_F 1.14 0.73
149_F 365_A 1.137 0.72
231_G 342_V 1.135 0.72
75_D 254_S 1.134 0.72
193_V 305_I 1.132 0.72
315_A 320_V 1.13 0.72
100_L 103_L 1.129 0.72
111_R 114_V 1.129 0.72
405_A 480_L 1.129 0.72
330_V 374_L 1.123 0.71
26_L 451_T 1.122 0.71
206_A 347_Q 1.12 0.71
130_A 332_L 1.118 0.71
438_L 445_V 1.118 0.71
585_E 589_H 1.117 0.71
409_R 499_D 1.114 0.70
566_K 573_K 1.114 0.70
541_R 545_D 1.112 0.70
197_L 200_R 1.111 0.70
537_P 541_R 1.11 0.70
151_V 328_F 1.108 0.70
128_A 159_A 1.107 0.70
42_G 511_V 1.107 0.70
155_Q 338_F 1.106 0.70
125_S 159_A 1.106 0.70
160_I 234_L 1.106 0.70
515_E 519_F 1.104 0.69
460_G 463_K 1.102 0.69
6_L 9_A 1.101 0.69
344_G 348_M 1.096 0.68
517_E 522_A 1.094 0.68
414_T 458_S 1.089 0.68
561_V 565_T 1.088 0.68
429_D 434_H 1.086 0.67
134_M 145_G 1.086 0.67
298_I 302_G 1.084 0.67
516_R 522_A 1.083 0.67
481_T 495_A 1.082 0.67
333_G 370_A 1.082 0.67
294_L 298_I 1.08 0.67
130_A 152_L 1.08 0.67
67_L 201_Y 1.079 0.67
435_I 447_Y 1.079 0.67
63_V 340_F 1.079 0.67
207_L 226_L 1.078 0.67
405_A 450_A 1.078 0.67
581_E 584_T 1.074 0.66
469_N 480_L 1.073 0.66
535_L 539_E 1.073 0.66
99_G 103_L 1.072 0.66
494_I 532_S 1.071 0.66
53_E 56_L 1.07 0.66
199_G 228_F 1.068 0.66
30_S 329_A 1.067 0.65
126_S 334_Q 1.065 0.65
542_E 545_D 1.063 0.65
149_F 155_Q 1.059 0.65
97_C 101_L 1.057 0.64
85_A 150_A 1.056 0.64
581_E 585_E 1.055 0.64
555_V 558_M 1.054 0.64
210_V 219_F 1.053 0.64
575_T 584_T 1.053 0.64
127_T 152_L 1.052 0.64
20_V 274_G 1.049 0.64
417_L 526_G 1.048 0.63
93_Q 152_L 1.047 0.63
261_L 266_E 1.046 0.63
520_E 559_A 1.045 0.63
557_E 561_V 1.043 0.63
535_L 541_R 1.042 0.63
484_V 488_F 1.042 0.63
538_Y 549_R 1.042 0.63
408_G 496_R 1.041 0.63
203_T 252_L 1.041 0.63
124_L 162_L 1.04 0.63
527_R 540_A 1.039 0.62
538_Y 544_A 1.039 0.62
471_I 477_N 1.038 0.62
70_I 127_T 1.038 0.62
294_L 305_I 1.037 0.62
552_I 582_I 1.036 0.62
528_L 531_E 1.035 0.62
230_F 251_L 1.034 0.62
548_R 552_I 1.033 0.62
513_K 552_I 1.031 0.62
570_A 574_R 1.029 0.61
227_V 270_G 1.029 0.61
124_L 371_T 1.026 0.61
370_A 437_T 1.025 0.61
282_M 408_G 1.025 0.61
578_M 581_E 1.025 0.61
118_I 360_L 1.025 0.61
566_K 570_A 1.024 0.61
450_A 480_L 1.022 0.61
425_M 443_M 1.021 0.60
560_M 563_N 1.021 0.60
304_L 374_L 1.02 0.60
39_C 351_V 1.02 0.60
124_L 159_A 1.019 0.60
347_Q 357_A 1.018 0.60
583_I 586_D 1.017 0.60
578_M 583_I 1.016 0.60
79_L 280_V 1.015 0.60
572_Y 576_S 1.014 0.60
550_F 579_L 1.013 0.60
405_A 484_V 1.012 0.59
99_G 467_L 1.012 0.59
302_G 305_I 1.012 0.59
70_I 231_G 1.011 0.59
453_M 461_A 1.01 0.59
219_F 257_Y 1.008 0.59
19_I 40_I 1.005 0.59
251_L 259_H 1.005 0.59
128_A 273_L 1.004 0.59
581_E 589_H 1.002 0.58
92_L 130_A 1.001 0.58
290_L 309_A 1 0.58
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4bwzA10.6291000.687Contact Map0.388
3l9wA20.36291000.775Contact Map0.71
4gx0A40.517799.90.86Contact Map0.656
1zcdA20.562999.90.863Contact Map0.2
1lnqA80.498499.90.864Contact Map0.67
2aefA20.343599.90.87Contact Map0.716
3c85A40.283999.90.872Contact Map0.547
3mt5A10.353299.90.876Contact Map0.586
3nafA10.411399.90.879Contact Map0.553
1id1A20.240399.90.879Contact Map0.655
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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