| GPCRAR |
| ID: | 1518214543 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 300 (275) | ||
| Sequences: | 1568 (1075.7) | ||
| Seq/Len: | 5.702 | ||
| Nf(neff/√len): | 64.9 |
HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 5.702).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 145_L | 204_L | 3.771 | 1.00 |
| 28_G | 32_L | 3.456 | 1.00 |
| 92_Y | 95_R | 3.366 | 1.00 |
| 92_Y | 98_D | 3.072 | 1.00 |
| 197_H | 202_Y | 2.833 | 1.00 |
| 245_F | 265_Q | 2.412 | 1.00 |
| 109_G | 113_V | 2.401 | 1.00 |
| 125_F | 129_S | 2.311 | 1.00 |
| 37_F | 142_A | 2.180 | 1.00 |
| 211_L | 235_G | 2.122 | 1.00 |
| 234_I | 272_I | 2.117 | 1.00 |
| 68_G | 113_V | 2.037 | 1.00 |
| 206_L | 239_F | 1.989 | 1.00 |
| 234_I | 276_V | 1.988 | 1.00 |
| 118_A | 128_V | 1.964 | 1.00 |
| 147_N | 152_E | 1.952 | 1.00 |
| 39_M | 62_L | 1.952 | 1.00 |
| 35_F | 66_F | 1.952 | 1.00 |
| 234_I | 275_G | 1.948 | 1.00 |
| 164_A | 195_P | 1.919 | 1.00 |
| 95_R | 98_D | 1.919 | 1.00 |
| 140_L | 232_F | 1.895 | 1.00 |
| 68_G | 109_G | 1.885 | 1.00 |
| 104_H | 264_G | 1.880 | 1.00 |
| 158_D | 164_A | 1.834 | 1.00 |
| 199_S | 243_V | 1.824 | 1.00 |
| 242_I | 246_F | 1.807 | 1.00 |
| 230_G | 278_M | 1.795 | 1.00 |
| 72_G | 109_G | 1.783 | 1.00 |
| 277_I | 281_W | 1.779 | 1.00 |
| 45_R | 48_R | 1.761 | 1.00 |
| 227_A | 283_Y | 1.738 | 1.00 |
| 230_G | 275_G | 1.720 | 1.00 |
| 239_F | 243_V | 1.707 | 1.00 |
| 90_P | 94_F | 1.663 | 1.00 |
| 91_L | 95_R | 1.650 | 1.00 |
| 64_A | 68_G | 1.642 | 1.00 |
| 41_L | 138_F | 1.640 | 1.00 |
| 75_I | 93_L | 1.617 | 0.99 |
| 18_S | 23_A | 1.610 | 0.99 |
| 154_W | 196_R | 1.609 | 0.99 |
| 144_R | 240_R | 1.608 | 0.99 |
| 54_T | 57_E | 1.582 | 0.99 |
| 196_R | 248_Q | 1.570 | 0.99 |
| 116_I | 120_R | 1.542 | 0.99 |
| 238_A | 272_I | 1.532 | 0.99 |
| 87_M | 90_P | 1.524 | 0.99 |
| 30_M | 33_V | 1.523 | 0.99 |
| 117_F | 121_T | 1.507 | 0.99 |
| 85_Q | 92_Y | 1.501 | 0.99 |
| 82_N | 85_Q | 1.501 | 0.99 |
| 241_I | 265_Q | 1.473 | 0.99 |
| 233_L | 275_G | 1.469 | 0.99 |
| 73_G | 109_G | 1.465 | 0.99 |
| 148_F | 201_L | 1.444 | 0.98 |
| 89_D | 92_Y | 1.431 | 0.98 |
| 152_E | 200_Q | 1.425 | 0.98 |
| 237_G | 272_I | 1.414 | 0.98 |
| 130_D | 226_G | 1.401 | 0.98 |
| 147_N | 153_L | 1.390 | 0.98 |
| 45_R | 219_I | 1.388 | 0.98 |
| 16_I | 26_W | 1.368 | 0.98 |
| 268_S | 271_M | 1.360 | 0.97 |
| 104_H | 268_S | 1.359 | 0.97 |
| 61_L | 128_V | 1.343 | 0.97 |
| 151_G | 168_P | 1.341 | 0.97 |
| 61_L | 65_G | 1.329 | 0.97 |
| 73_G | 106_G | 1.325 | 0.97 |
| 91_L | 94_F | 1.315 | 0.97 |
| 85_Q | 89_D | 1.295 | 0.96 |
| 47_N | 55_K | 1.275 | 0.96 |
| 129_S | 136_I | 1.262 | 0.96 |
| 61_L | 114_M | 1.256 | 0.95 |
| 113_V | 116_I | 1.252 | 0.95 |
| 35_F | 106_G | 1.250 | 0.95 |
| 38_A | 139_G | 1.237 | 0.95 |
| 19_I | 22_V | 1.232 | 0.95 |
| 238_A | 242_I | 1.227 | 0.95 |
| 53_W | 131_F | 1.224 | 0.95 |
| 224_P | 227_A | 1.219 | 0.94 |
| 157_V | 194_L | 1.217 | 0.94 |
| 208_G | 211_L | 1.212 | 0.94 |
| 29_L | 33_V | 1.209 | 0.94 |
| 208_G | 212_F | 1.206 | 0.94 |
| 278_M | 282_A | 1.203 | 0.94 |
| 137_P | 235_G | 1.202 | 0.94 |
| 228_V | 231_L | 1.185 | 0.93 |
| 31_Y | 34_G | 1.179 | 0.93 |
| 209_V | 213_I | 1.177 | 0.93 |
| 138_F | 212_F | 1.169 | 0.93 |
| 147_N | 150_N | 1.167 | 0.93 |
| 74_R | 99_G | 1.154 | 0.92 |
| 134_P | 137_P | 1.151 | 0.92 |
| 241_I | 245_F | 1.140 | 0.91 |
| 36_I | 40_W | 1.135 | 0.91 |
| 161_F | 164_A | 1.134 | 0.91 |
| 64_A | 113_V | 1.127 | 0.91 |
| 284_R | 287_P | 1.124 | 0.91 |
| 238_A | 241_I | 1.124 | 0.91 |
| 102_S | 153_L | 1.123 | 0.91 |
| 57_E | 123_R | 1.122 | 0.91 |
| 104_H | 237_G | 1.113 | 0.90 |
| 64_A | 117_F | 1.110 | 0.90 |
| 103_F | 140_L | 1.110 | 0.90 |
| 212_F | 216_N | 1.107 | 0.90 |
| 78_V | 93_L | 1.106 | 0.90 |
| 151_G | 200_Q | 1.105 | 0.90 |
| 199_S | 262_S | 1.103 | 0.90 |
| 237_G | 271_M | 1.102 | 0.89 |
| 210_V | 235_G | 1.102 | 0.89 |
| 27_Y | 152_E | 1.098 | 0.89 |
| 38_A | 135_L | 1.084 | 0.88 |
| 14_P | 25_H | 1.082 | 0.88 |
| 138_F | 211_L | 1.082 | 0.88 |
| 213_I | 217_L | 1.077 | 0.88 |
| 90_P | 93_L | 1.075 | 0.88 |
| 147_N | 200_Q | 1.074 | 0.88 |
| 58_V | 128_V | 1.063 | 0.87 |
| 63_Y | 67_L | 1.062 | 0.87 |
| 17_F | 26_W | 1.050 | 0.86 |
| 212_F | 215_L | 1.044 | 0.86 |
| 43_T | 59_E | 1.041 | 0.86 |
| 169_G | 172_T | 1.035 | 0.85 |
| 16_I | 25_H | 1.034 | 0.85 |
| 41_L | 216_N | 1.032 | 0.85 |
| 26_W | 149_I | 1.032 | 0.85 |
| 77_Y | 80_F | 1.025 | 0.84 |
| 157_V | 196_R | 1.024 | 0.84 |
| 279_M | 283_Y | 1.022 | 0.84 |
| 133_A | 229_S | 1.018 | 0.84 |
| 112_V | 116_I | 1.014 | 0.83 |
| 160_N | 164_A | 1.014 | 0.83 |
| 153_L | 264_G | 1.012 | 0.83 |
| 74_R | 101_M | 1.005 | 0.83 |
| 16_I | 29_L | 1.004 | 0.83 |
| 207_E | 239_F | 1.002 | 0.82 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 2ls4A | 1 | 0.08 | 3.2 | 0.972 | Contact Map |
| 2kluA | 1 | 0.1667 | 2.6 | 0.974 | Contact Map |
| 4px7A | 1 | 0.7467 | 2 | 0.975 | Contact Map |
| 2looA | 1 | 0.33 | 1.9 | 0.975 | Contact Map |
| 2m20A | 2 | 0.1433 | 1.5 | 0.977 | Contact Map |
| 3wu2Y | 2 | 0.0933 | 1.3 | 0.978 | Contact Map |
| 4us3A | 1 | 0.45 | 1.3 | 0.978 | Contact Map |
| 3aqpA | 2 | 0.1833 | 1.3 | 0.978 | Contact Map |
| 4he8J | 2 | 0.15 | 1.2 | 0.978 | Contact Map |
| 2jo1A | 1 | 0.1767 | 1.1 | 0.979 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)