OPENSEQ.org
Trial Subtilisin

ID: 1515622456 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 383 (349)
Sequences: 9324 (6660)
Seq/Len: 26.716
Nf(neff/√len): 356.5

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 26.716).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
102_D213_S3.1831.00
136_V180_I3.1281.00
287_N295_N3.1211.00
317_V323_T3.0611.00
204_L210_G3.0601.00
338_A378_V3.0211.00
286_A307_S2.9161.00
130_K195_S2.7821.00
231_N332_S2.6291.00
295_N311_T2.5811.00
175_H330_M2.5751.00
317_V325_Y2.5541.00
169_F206_R2.5451.00
297_S311_T2.5211.00
132_A195_S2.4651.00
338_A376_I2.4061.00
178_G315_S2.3661.00
176_V231_N2.3591.00
363_T377_N2.3441.00
201_V218_G2.3131.00
248_D281_S2.2251.00
42_S102_D2.1701.00
178_G316_T2.1611.00
175_H315_S2.1341.00
292_N375_L2.1021.00
138_V180_I2.0821.00
168_D171_S2.0811.00
260_A332_S2.0711.00
256_V339_A2.0651.00
135_K197_S2.0491.00
241_T245_N2.0481.00
287_N297_S2.0461.00
289_N311_T2.0371.00
135_K199_Y2.0211.00
332_S336_A2.0081.00
277_A284_A2.0071.00
350_S353_Q1.9931.00
179_T334_H1.9841.00
182_A316_T1.9831.00
139_L203_V1.9631.00
361_T381_A1.9001.00
219_I250_A1.8731.00
256_V283_I1.8511.00
223_V250_A1.8471.00
181_A198_L1.8121.00
258_V332_S1.7791.00
83_S86_A1.7571.00
105_A212_Y1.7411.00
48_K97_A1.7121.00
356_Q360_N1.7101.00
179_T330_M1.7071.00
355_R359_E1.7021.00
181_A196_A1.6991.00
137_A222_A1.6991.00
287_N311_T1.6921.00
118_Q292_N1.6911.00
46_G97_A1.6881.00
201_V221_W1.6851.00
143_I198_L1.6621.00
138_V176_V1.6261.00
115_G334_H1.6231.00
306_V358_L1.6021.00
250_A257_V1.5941.00
346_Y349_L1.5871.00
211_Q214_W1.5851.00
46_G98_Y1.5831.00
339_A354_V1.5821.00
289_N293_V1.5771.00
286_A304_L1.5591.00
129_Y344_S1.5571.00
223_V255_V1.5571.00
244_K278_K1.5481.00
306_V376_I1.5461.00
248_D252_N1.5421.00
136_V196_A1.5331.00
346_Y357_R1.5331.00
314_L324_S1.5301.00
108_Y260_A1.5101.00
265_G294_R1.5071.00
100_E242_A1.5031.00
137_A227_M1.4881.00
216_I246_A1.4831.00
228_D343_L1.4811.00
192_V337_G1.4721.00
287_N310_G1.4701.00
294_R307_S1.4691.00
231_N336_A1.4681.00
134_V343_L1.4671.00
153_A197_S1.4611.00
70_K217_S1.4501.00
136_V340_A1.4451.00
325_Y330_M1.4421.00
144_H318_P1.4351.00
343_L354_V1.4181.00
244_K248_D1.4101.00
258_V339_A1.4060.99
120_K377_N1.4050.99
203_V234_L1.3790.99
256_V354_V1.3750.99
249_T252_N1.3750.99
248_D280_D1.3740.99
349_L357_R1.3520.99
199_Y227_M1.3450.99
272_T303_E1.3420.99
215_I234_L1.3410.99
294_R298_S1.3350.99
134_V340_A1.3270.99
342_I349_L1.3260.99
221_W225_N1.3250.99
130_K344_S1.3240.99
260_A331_A1.3140.99
216_I242_A1.3130.99
377_N380_A1.3110.99
229_V336_A1.3060.99
175_H325_Y1.3060.99
176_V333_P1.2980.99
177_A200_A1.2800.99
258_V335_V1.2750.99
203_V218_G1.2740.99
175_H179_T1.2690.99
335_V376_I1.2650.99
288_V307_S1.2520.99
87_A91_E1.2300.98
258_V336_A1.2160.98
339_A358_L1.1950.98
364_P368_S1.1940.98
139_L218_G1.1900.98
248_D279_Y1.1860.98
342_I381_A1.1840.98
100_E240_S1.1780.98
220_E224_A1.1710.98
230_I257_V1.1700.97
264_S274_G1.1690.97
315_S330_M1.1680.97
153_A199_Y1.1590.97
309_P313_I1.1560.97
286_A298_S1.1560.97
193_A340_A1.1540.97
42_S81_K1.1480.97
264_S299_S1.1450.97
297_S326_T1.1440.97
247_V282_T1.1400.97
283_I355_R1.1340.97
174_T316_T1.1140.96
338_A358_L1.1130.96
119_I192_V1.1110.96
219_I232_M1.0980.96
359_E373_K1.0950.96
294_R300_A1.0910.96
141_T170_Q1.0900.96
305_D359_E1.0880.96
379_Q383_N1.0880.96
107_A204_L1.0860.96
285_V306_V1.0850.96
223_V253_R1.0790.95
103_H212_Y1.0780.95
47_F78_A1.0770.95
149_D316_T1.0750.95
311_T326_T1.0730.95
261_A284_A1.0650.95
256_V351_T1.0640.95
143_I177_A1.0590.95
96_I99_V1.0590.95
220_E249_T1.0590.95
177_A198_L1.0570.95
219_I230_I1.0550.95
61_V66_G1.0520.95
157_S160_P1.0520.95
357_R361_T1.0510.94
154_G199_Y1.0490.94
145_A152_V1.0480.94
300_A370_Y1.0480.94
127_Q379_Q1.0480.94
259_A282_T1.0470.94
285_V335_V1.0350.94
365_L375_L1.0260.94
339_A342_I1.0230.93
230_I250_A1.0210.93
145_A167_Q1.0190.93
279_Y282_T1.0180.93
288_V292_N1.0150.93
69_E81_K1.0140.93
148_P320_S1.0130.93
233_S333_P1.0120.93
157_S202_K1.0110.93
219_I257_V1.0020.92
143_I200_A1.0020.92
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
3whiA20.90081000.173Contact Map
3wiuA30.87471000.24Contact Map
1r6vA10.9531000.247Contact Map
3afgA20.94521000.248Contact Map
3t41A20.88251000.256Contact Map
2p4eP20.86161000.269Contact Map
4tr2A20.89821000.29Contact Map
1to2E10.7181000.296Contact Map
1r0rE10.71541000.303Contact Map
1gciA10.70231000.307Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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