| SLC35F2 |
| ID: | 1515509291 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 374 (307) | ||
| Sequences: | 10828 (7572.7) | ||
| Seq/Len: | 35.270 | ||
| Nf(neff/√len): | 432.2 |
HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 35.270).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 128_A | 202_V | 3.548 | 1.00 |
| 167_A | 328_G | 2.789 | 1.00 |
| 149_M | 168_V | 2.698 | 1.00 |
| 281_L | 285_V | 2.663 | 1.00 |
| 46_G | 282_V | 2.651 | 1.00 |
| 200_I | 204_L | 2.651 | 1.00 |
| 124_V | 204_L | 2.518 | 1.00 |
| 45_L | 281_L | 2.500 | 1.00 |
| 84_F | 237_T | 2.473 | 1.00 |
| 84_F | 234_L | 2.392 | 1.00 |
| 158_A | 217_E | 2.347 | 1.00 |
| 147_V | 206_A | 2.336 | 1.00 |
| 124_V | 201_L | 2.302 | 1.00 |
| 88_T | 230_G | 2.157 | 1.00 |
| 81_C | 237_T | 2.154 | 1.00 |
| 281_L | 284_K | 2.121 | 1.00 |
| 77_F | 241_G | 2.113 | 1.00 |
| 136_S | 199_D | 2.111 | 1.00 |
| 127_R | 201_L | 2.100 | 1.00 |
| 41_K | 285_V | 2.099 | 1.00 |
| 306_G | 312_Y | 2.057 | 1.00 |
| 196_L | 200_I | 2.017 | 1.00 |
| 132_T | 198_G | 1.949 | 1.00 |
| 153_W | 160_Y | 1.906 | 1.00 |
| 79_N | 272_M | 1.874 | 1.00 |
| 54_C | 301_Y | 1.859 | 1.00 |
| 140_L | 202_V | 1.797 | 1.00 |
| 131_Y | 197_I | 1.769 | 1.00 |
| 177_T | 324_V | 1.763 | 1.00 |
| 104_L | 107_K | 1.751 | 1.00 |
| 152_S | 158_A | 1.728 | 1.00 |
| 51_L | 301_Y | 1.724 | 1.00 |
| 38_N | 41_K | 1.694 | 1.00 |
| 171_C | 296_L | 1.683 | 1.00 |
| 287_S | 290_S | 1.668 | 1.00 |
| 132_T | 199_D | 1.667 | 1.00 |
| 44_A | 48_M | 1.658 | 1.00 |
| 124_V | 208_L | 1.613 | 1.00 |
| 115_G | 235_F | 1.610 | 1.00 |
| 147_V | 210_A | 1.603 | 1.00 |
| 83_L | 233_G | 1.602 | 1.00 |
| 316_G | 320_L | 1.585 | 1.00 |
| 60_S | 126_V | 1.574 | 1.00 |
| 45_L | 278_F | 1.562 | 1.00 |
| 151_L | 214_V | 1.562 | 1.00 |
| 143_F | 147_V | 1.556 | 1.00 |
| 151_L | 155_I | 1.531 | 1.00 |
| 297_T | 301_Y | 1.524 | 1.00 |
| 169_A | 173_L | 1.524 | 1.00 |
| 134_L | 306_G | 1.522 | 1.00 |
| 171_C | 328_G | 1.519 | 1.00 |
| 283_I | 287_S | 1.504 | 1.00 |
| 330_I | 334_S | 1.502 | 1.00 |
| 54_C | 305_V | 1.494 | 1.00 |
| 55_G | 265_F | 1.489 | 1.00 |
| 58_I | 305_V | 1.483 | 1.00 |
| 167_A | 332_Y | 1.483 | 1.00 |
| 218_Y | 221_K | 1.473 | 1.00 |
| 301_Y | 305_V | 1.461 | 1.00 |
| 173_L | 177_T | 1.440 | 1.00 |
| 49_L | 282_V | 1.437 | 1.00 |
| 40_L | 44_A | 1.423 | 1.00 |
| 77_F | 81_C | 1.420 | 1.00 |
| 147_V | 207_S | 1.411 | 1.00 |
| 110_K | 219_I | 1.411 | 1.00 |
| 48_M | 278_F | 1.400 | 0.99 |
| 165_F | 169_A | 1.396 | 0.99 |
| 314_F | 319_I | 1.396 | 0.99 |
| 174_G | 325_I | 1.394 | 0.99 |
| 133_T | 136_S | 1.392 | 0.99 |
| 151_L | 210_A | 1.392 | 0.99 |
| 177_T | 317_L | 1.391 | 0.99 |
| 148_L | 288_A | 1.390 | 0.99 |
| 152_S | 156_L | 1.381 | 0.99 |
| 132_T | 136_S | 1.379 | 0.99 |
| 174_G | 321_S | 1.370 | 0.99 |
| 61_Q | 312_Y | 1.363 | 0.99 |
| 161_R | 164_H | 1.357 | 0.99 |
| 140_L | 203_L | 1.357 | 0.99 |
| 127_R | 130_Q | 1.356 | 0.99 |
| 137_V | 202_V | 1.351 | 0.99 |
| 286_T | 290_S | 1.348 | 0.99 |
| 148_L | 210_A | 1.336 | 0.99 |
| 47_Q | 297_T | 1.333 | 0.99 |
| 77_F | 237_T | 1.326 | 0.99 |
| 138_Q | 302_S | 1.318 | 0.99 |
| 140_L | 206_A | 1.317 | 0.99 |
| 88_T | 234_L | 1.313 | 0.99 |
| 131_Y | 201_L | 1.307 | 0.99 |
| 162_V | 166_I | 1.304 | 0.99 |
| 82_L | 267_A | 1.300 | 0.99 |
| 224_S | 227_E | 1.298 | 0.99 |
| 238_I | 242_I | 1.286 | 0.99 |
| 163_I | 331_L | 1.285 | 0.99 |
| 120_E | 208_L | 1.280 | 0.99 |
| 37_W | 41_K | 1.273 | 0.99 |
| 109_W | 113_L | 1.270 | 0.99 |
| 174_G | 328_G | 1.265 | 0.99 |
| 84_F | 88_T | 1.263 | 0.99 |
| 178_M | 321_S | 1.259 | 0.99 |
| 128_A | 132_T | 1.256 | 0.99 |
| 148_L | 214_V | 1.234 | 0.98 |
| 319_I | 323_T | 1.231 | 0.98 |
| 89_V | 92_A | 1.217 | 0.98 |
| 305_V | 309_L | 1.212 | 0.98 |
| 160_Y | 165_F | 1.202 | 0.98 |
| 323_T | 327_V | 1.197 | 0.98 |
| 53_I | 279_M | 1.192 | 0.98 |
| 84_F | 230_G | 1.191 | 0.98 |
| 197_I | 201_L | 1.175 | 0.98 |
| 216_E | 280_P | 1.171 | 0.98 |
| 307_L | 314_F | 1.169 | 0.97 |
| 134_L | 310_F | 1.161 | 0.97 |
| 118_D | 212_S | 1.153 | 0.97 |
| 147_V | 203_L | 1.153 | 0.97 |
| 86_I | 273_F | 1.149 | 0.97 |
| 196_L | 199_D | 1.146 | 0.97 |
| 162_V | 165_F | 1.144 | 0.97 |
| 106_R | 110_K | 1.143 | 0.97 |
| 156_L | 214_V | 1.137 | 0.97 |
| 334_S | 338_R | 1.126 | 0.97 |
| 173_L | 324_V | 1.123 | 0.97 |
| 77_F | 244_L | 1.114 | 0.96 |
| 316_G | 319_I | 1.110 | 0.96 |
| 257_W | 261_I | 1.107 | 0.96 |
| 153_W | 159_R | 1.101 | 0.96 |
| 117_A | 211_I | 1.098 | 0.96 |
| 154_F | 157_H | 1.096 | 0.96 |
| 151_L | 211_I | 1.095 | 0.96 |
| 127_R | 131_Y | 1.092 | 0.96 |
| 163_I | 334_S | 1.086 | 0.96 |
| 198_G | 202_V | 1.080 | 0.95 |
| 213_N | 283_I | 1.076 | 0.95 |
| 43_I | 294_G | 1.070 | 0.95 |
| 135_T | 303_L | 1.069 | 0.95 |
| 282_V | 291_V | 1.066 | 0.95 |
| 286_T | 291_V | 1.065 | 0.95 |
| 112_I | 235_F | 1.057 | 0.95 |
| 267_A | 273_F | 1.055 | 0.95 |
| 110_K | 218_Y | 1.049 | 0.94 |
| 180_G | 315_S | 1.040 | 0.94 |
| 149_M | 153_W | 1.039 | 0.94 |
| 107_K | 222_K | 1.037 | 0.94 |
| 139_L | 179_V | 1.037 | 0.94 |
| 151_L | 154_F | 1.032 | 0.94 |
| 107_K | 223_L | 1.031 | 0.94 |
| 113_L | 215_C | 1.030 | 0.94 |
| 170_V | 328_G | 1.028 | 0.94 |
| 315_S | 319_I | 1.027 | 0.94 |
| 131_Y | 198_G | 1.025 | 0.93 |
| 53_I | 275_L | 1.023 | 0.93 |
| 104_L | 108_W | 1.019 | 0.93 |
| 110_K | 215_C | 1.016 | 0.93 |
| 258_D | 261_I | 1.016 | 0.93 |
| 315_S | 318_Y | 1.014 | 0.93 |
| 197_I | 200_I | 1.014 | 0.93 |
| 76_S | 126_V | 1.013 | 0.93 |
| 87_Y | 273_F | 1.012 | 0.93 |
| 125_I | 129_Y | 1.011 | 0.93 |
| 105_K | 109_W | 1.003 | 0.93 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 2i68A | 2 | 0.0214 | 98.7 | 0.838 | Contact Map |
| 3b5dA | 2 | 0.016 | 98.1 | 0.867 | Contact Map |
| 4j05A | 2 | 0.8877 | 63.4 | 0.941 | Contact Map |
| 4pypA | 1 | 0.8583 | 54.6 | 0.944 | Contact Map |
| 4tphA | 2 | 0.8048 | 33.2 | 0.951 | Contact Map |
| 3wdoA | 1 | 0.8235 | 32.5 | 0.951 | Contact Map |
| 4zw9A | 1 | 0 | 21.5 | 0.955 | Contact Map |
| 4ldsA | 2 | 0.5321 | 20.3 | 0.956 | Contact Map |
| 2cfqA | 1 | 0.5535 | 20.2 | 0.956 | Contact Map |
| 1pw4A | 1 | 0.885 | 16.3 | 0.958 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)