OPENSEQ.org
prmt5 elegans 1-300 42-297

ID: 1515502909 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 256 (250)
Sequences: 315 (231.5)
Seq/Len: 1.260
Nf(neff/√len): 14.6

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 1.260).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
84_G119_L3.8231.00
222_L253_L2.7201.00
249_L253_L1.9570.97
235_G247_I1.9550.97
90_I103_S1.9220.96
89_W103_S1.9190.96
225_F253_L1.8780.95
121_T152_W1.8740.95
210_E249_L1.8320.95
176_W179_W1.8300.95
127_T134_T1.8080.94
176_W183_R1.7600.93
65_V68_L1.7000.91
204_L246_H1.6820.91
142_I194_F1.6580.90
126_L134_T1.5970.87
180_A183_R1.5720.86
179_W183_R1.5670.86
217_W220_E1.5550.85
244_S247_I1.5280.84
133_R136_A1.5180.83
233_I243_P1.5130.83
117_L224_A1.4880.82
84_G122_M1.4780.81
89_W110_E1.4710.81
235_G242_I1.4610.80
90_I110_E1.4570.80
89_W106_H1.4220.78
92_C123_A1.3740.74
107_L111_L1.3600.73
235_G238_G1.3520.72
19_A209_T1.3130.69
83_V121_T1.3120.69
179_W221_P1.3060.69
103_S110_E1.3000.68
195_Q223_A1.2660.65
235_G240_A1.2650.65
68_L78_W1.2640.65
30_C236_R1.2610.65
114_I235_G1.2580.64
243_P247_I1.2490.64
194_F208_L1.2470.63
104_E136_A1.2350.62
66_I133_R1.2280.62
52_F55_P1.2200.61
183_R186_C1.2150.60
73_L94_S1.2030.59
125_E204_L1.2030.59
237_N241_S1.2020.59
153_V174_D1.1990.59
73_L80_S1.1990.59
51_A55_P1.1990.59
222_L236_R1.1980.59
26_V68_L1.1840.57
125_E154_Q1.1800.57
85_K123_A1.1780.57
238_G247_I1.1730.56
205_P246_H1.1680.56
48_V84_G1.1600.55
63_P144_T1.1540.55
51_A54_T1.1510.54
199_T228_E1.1460.54
11_W163_K1.1440.54
107_L153_V1.1410.53
240_A244_S1.1400.53
8_H80_S1.1390.53
183_R188_N1.1390.53
124_I255_T1.1280.52
124_I153_V1.1280.52
90_I156_P1.1270.52
42_V126_L1.1270.52
235_G244_S1.1230.51
70_D91_D1.1210.51
21_N209_T1.1210.51
70_D73_L1.1160.51
95_S147_S1.1070.50
174_D237_N1.1060.50
195_Q239_E1.1050.50
40_F120_Q1.1030.49
174_D216_R1.0920.48
121_T229_S1.0920.48
64_P67_D1.0830.48
95_S196_V1.0820.47
10_G127_T1.0820.47
36_F112_S1.0800.47
156_P222_L1.0790.47
87_S90_I1.0750.47
40_F152_W1.0730.47
235_G241_S1.0710.46
110_E217_W1.0620.45
33_L38_Y1.0620.45
110_E113_Y1.0600.45
237_N243_P1.0590.45
79_E118_G1.0540.45
12_M113_Y1.0530.45
108_L157_S1.0440.44
52_F56_N1.0430.44
59_A227_I1.0430.44
184_K188_N1.0410.43
51_A69_P1.0410.43
222_L230_G1.0390.43
9_I17_D1.0390.43
83_V152_W1.0370.43
118_G254_W1.0340.43
72_Q219_A1.0320.43
13_A105_E1.0220.42
64_P68_L1.0210.41
73_L237_N1.0170.41
54_T64_P1.0150.41
153_V182_F1.0140.41
217_W221_P1.0130.41
229_S234_S1.0090.40
75_N116_Y1.0020.40
44_P73_L1.0010.40
177_T218_K1.0010.40
118_G157_S1.0000.39
38_Y148_R1.0000.39
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
3ua3A20.97271000.169Contact Map
4gqbA10.92971000.2Contact Map
3aalA10.871151.50.952Contact Map
4hnoA10.859448.30.953Contact Map
3aamA10.863330.20.958Contact Map
1qtwA10.855529.60.959Contact Map
3ktcA20.910228.80.959Contact Map
2zdsA60.867227.80.959Contact Map
2j6vA20.867226.90.959Contact Map
1i60A10.867225.10.96Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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