| fbab |
| ID: | 1511887385 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 121 (105) | ||
| Sequences: | 1987 (1659) | ||
| Seq/Len: | 18.924 | ||
| Nf(neff/√len): | 161.9 |
Jackhmmer Results: (2016_04)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 18.924).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 46_T | 82_A | 4.067 | 1.00 |
| 45_A | 84_P | 3.655 | 1.00 |
| 31_K | 65_Q | 2.931 | 1.00 |
| 50_R | 79_V | 2.889 | 1.00 |
| 79_V | 92_A | 2.757 | 1.00 |
| 75_K | 96_T | 2.584 | 1.00 |
| 51_D | 55_K | 2.465 | 1.00 |
| 48_E | 81_T | 2.305 | 1.00 |
| 50_R | 77_T | 2.233 | 1.00 |
| 49_L | 68_D | 2.199 | 1.00 |
| 34_K | 80_E | 2.146 | 1.00 |
| 32_F | 66_V | 2.141 | 1.00 |
| 20_M | 24_E | 2.137 | 1.00 |
| 49_L | 78_F | 1.946 | 1.00 |
| 45_A | 83_A | 1.876 | 1.00 |
| 33_S | 41_E | 1.850 | 1.00 |
| 78_F | 95_F | 1.849 | 1.00 |
| 48_E | 79_V | 1.832 | 1.00 |
| 31_K | 67_K | 1.801 | 1.00 |
| 90_A | 93_I | 1.670 | 1.00 |
| 46_T | 59_T | 1.657 | 1.00 |
| 21_T | 24_E | 1.657 | 1.00 |
| 75_K | 94_T | 1.653 | 1.00 |
| 33_S | 65_Q | 1.644 | 1.00 |
| 30_I | 95_F | 1.592 | 1.00 |
| 50_R | 56_T | 1.536 | 1.00 |
| 32_F | 47_M | 1.513 | 1.00 |
| 29_H | 69_F | 1.490 | 1.00 |
| 77_T | 94_T | 1.419 | 1.00 |
| 46_T | 81_T | 1.404 | 0.99 |
| 83_A | 89_V | 1.398 | 0.99 |
| 77_T | 92_A | 1.385 | 0.99 |
| 60_W | 66_V | 1.315 | 0.99 |
| 51_D | 72_M | 1.304 | 0.99 |
| 60_W | 64_G | 1.210 | 0.98 |
| 103_V | 107_G | 1.187 | 0.98 |
| 47_M | 60_W | 1.143 | 0.97 |
| 36_D | 40_K | 1.138 | 0.97 |
| 29_H | 67_K | 1.138 | 0.97 |
| 30_I | 78_F | 1.134 | 0.97 |
| 42_L | 84_P | 1.132 | 0.97 |
| 98_N | 101_G | 1.128 | 0.97 |
| 32_F | 78_F | 1.104 | 0.96 |
| 20_M | 23_E | 1.080 | 0.95 |
| 32_F | 60_W | 1.053 | 0.95 |
| 35_R | 39_G | 1.041 | 0.94 |
| 74_G | 97_V | 1.033 | 0.94 |
| 36_D | 87_Y | 1.031 | 0.94 |
| 18_G | 23_E | 1.028 | 0.94 |
| 71_L | 76_Y | 1.010 | 0.93 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 2x5pA | 1 | 0.8595 | 99.9 | 0.124 | Contact Map |
| 2ww8A | 1 | 0.7934 | 99.7 | 0.36 | Contact Map |
| 4oq1A | 1 | 1 | 99.7 | 0.398 | Contact Map |
| 2xi9A | 2 | 0.7273 | 99.6 | 0.425 | Contact Map |
| 3kptA | 2 | 0.8347 | 99.6 | 0.447 | Contact Map |
| 3phsA | 1 | 0.9091 | 99.6 | 0.45 | Contact Map |
| 4p0dA | 1 | 0.9835 | 99.6 | 0.45 | Contact Map |
| 4uzgA | 1 | 0 | 99.5 | 0.461 | Contact Map |
| 3txaA | 1 | 0.0248 | 99.5 | 0.467 | Contact Map |
| 2xtlA | 2 | 0.9339 | 99.5 | 0.471 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)