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ID: 1510150714 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 757 (751)
Sequences: 988 (666.4)
Seq/Len: 1.316
Nf(neff/√len): 24.3

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 1.316).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
15_T18_D4.8681.00
10_I36_L3.8981.00
17_F70_E3.8571.00
598_L753_L3.8181.00
602_L700_A3.7131.00
615_R634_A3.5461.00
663_A753_L3.5041.00
701_L746_F3.4901.00
105_V120_I3.4691.00
133_V158_F3.3591.00
615_R637_L3.2061.00
23_V78_R3.1081.00
690_L742_V3.0111.00
16_L67_G2.9821.00
291_V691_I2.9431.00
729_D734_R2.8301.00
221_R225_E2.8221.00
541_A561_L2.7831.00
302_T432_R2.6381.00
598_L663_A2.5011.00
534_E566_T2.4701.00
41_E57_R2.4541.00
65_P69_A2.4030.99
69_A225_E2.3980.99
58_V68_L2.3820.99
694_D699_I2.3680.99
24_P78_R2.2780.99
241_A244_D2.2680.99
435_S486_I2.2510.99
684_G718_T2.2440.99
36_L75_A2.2170.99
495_R584_E2.1910.99
41_E60_A2.1850.99
479_S617_D2.1840.99
27_H80_M2.1840.99
292_L595_A2.1760.99
537_A565_V2.1760.99
243_G273_Q2.1720.99
104_D108_R2.1610.99
538_L562_T2.1490.99
691_I702_A2.1260.98
117_Y214_D2.1160.98
23_V75_A2.1110.98
86_I233_V2.0910.98
220_G252_L2.0780.98
298_E446_F2.0400.98
68_L227_L2.0300.98
595_A702_A1.9910.97
34_L71_L1.9880.97
8_L19_A1.9880.97
701_L720_V1.9840.97
298_E302_T1.9810.97
619_S627_R1.9790.97
64_G67_G1.9790.97
136_D140_E1.9780.97
729_D732_T1.9730.97
84_V228_V1.9700.97
24_P635_S1.9570.97
138_P170_R1.9560.97
74_A78_R1.9440.97
507_I574_V1.9410.97
170_R174_D1.9370.97
617_D634_A1.9350.97
471_L600_Q1.9280.97
293_V436_L1.9120.96
701_L745_L1.9010.96
290_R448_I1.8820.96
102_W202_A1.8760.96
518_I537_A1.8700.96
690_L701_L1.8620.96
219_I231_L1.8570.95
6_Y26_L1.8560.95
534_E562_T1.8410.95
515_E519_W1.8240.95
116_D208_D1.8100.95
454_H677_Y1.8030.94
17_F20_A1.8020.94
240_R408_L1.7920.94
104_D113_E1.7920.94
433_L486_I1.7870.94
215_T219_I1.7830.94
435_S487_T1.7830.94
503_V577_L1.7780.94
93_V202_A1.7510.93
522_I533_L1.7420.93
685_A717_D1.7200.92
680_V699_I1.7190.92
28_R642_D1.7080.92
382_Q393_A1.7080.92
65_P221_R1.6900.91
507_I519_W1.6730.91
250_A277_A1.6720.91
315_W426_A1.6710.91
323_K418_M1.6700.91
197_A330_I1.6690.91
2_P257_P1.6640.91
160_I164_T1.6550.90
434_T517_L1.6440.90
703_T706_P1.6400.90
324_R355_E1.6370.90
27_H78_R1.6260.89
307_E501_T1.6250.89
519_W564_L1.6230.89
601_L609_V1.6200.89
472_T479_S1.6180.89
305_V513_V1.6120.89
141_L149_D1.6100.89
136_D141_L1.6070.89
44_S98_A1.6030.88
218_E222_W1.6000.88
304_I497_R1.5990.88
23_V71_L1.5990.88
243_G247_R1.5860.88
16_L39_L1.5840.88
559_E563_R1.5760.87
703_T749_Y1.5750.87
450_A691_I1.5670.87
692_G701_L1.5620.87
2_P259_W1.5580.86
20_A23_V1.5580.86
715_W718_T1.5470.86
555_P559_E1.5440.86
357_A417_I1.5320.85
682_A690_L1.5310.85
284_A469_T1.5190.84
499_F503_V1.5130.84
488_A585_L1.5070.84
289_D596_A1.5060.84
250_A258_I1.5040.84
502_E577_L1.4950.83
471_L480_E1.4900.83
581_L585_L1.4800.82
86_I219_I1.4780.82
615_R657_T1.4780.82
286_A441_G1.4740.82
590_Y650_D1.4540.81
75_A80_M1.4510.81
530_R566_T1.4480.80
698_A753_L1.4470.80
521_A533_L1.4420.80
209_P248_R1.4400.80
642_D665_R1.4390.80
365_S411_Q1.4350.80
519_W574_V1.4280.79
10_I75_A1.4190.78
615_R633_A1.4180.78
542_R558_E1.4180.78
33_W229_D1.4150.78
480_E600_Q1.4140.78
633_A637_L1.4100.78
16_L68_L1.4080.78
428_Y621_V1.4030.77
663_A698_A1.3960.77
504_G515_E1.3880.76
17_F67_G1.3820.76
14_F61_A1.3770.75
602_L693_F1.3760.75
55_H100_R1.3740.75
436_L446_F1.3670.75
57_R60_A1.3590.74
43_E200_T1.3570.74
356_I410_F1.3510.74
114_Y117_Y1.3480.73
4_S32_D1.3480.73
65_P218_E1.3470.73
283_D468_M1.3430.73
284_A288_I1.3390.73
287_G456_A1.3250.71
685_A716_G1.3200.71
308_A504_G1.3190.71
137_G182_S1.3190.71
599_L603_A1.3190.71
491_E592_N1.3190.71
28_R635_S1.3160.71
39_L82_V1.3160.71
297_G301_L1.3160.71
680_V720_V1.3160.71
682_A720_V1.3150.71
425_N545_G1.3120.70
693_F700_A1.3120.70
616_W653_G1.3050.70
318_L426_A1.3050.70
283_D467_S1.3030.70
523_V581_L1.3030.70
562_T566_T1.2950.69
523_V582_V1.2950.69
457_Q603_A1.2930.69
336_R350_E1.2840.68
169_P172_V1.2810.68
108_R162_E1.2800.68
102_W526_W1.2800.68
284_A603_A1.2760.67
613_T617_D1.2730.67
673_L698_A1.2720.67
741_R744_E1.2720.67
597_K660_V1.2690.67
415_G418_M1.2690.67
319_V422_V1.2670.66
74_A77_E1.2620.66
682_A692_G1.2600.66
76_H229_D1.2600.66
436_L447_A1.2580.66
39_L84_V1.2580.66
380_A396_G1.2560.66
101_W117_Y1.2530.65
482_T524_G1.2510.65
322_R429_R1.2490.65
289_D435_S1.2460.65
661_S665_R1.2370.64
514_L557_F1.2340.63
72_S227_L1.2340.63
700_A753_L1.2320.63
637_L654_A1.2310.63
218_E221_R1.2300.63
161_A177_H1.2300.63
669_D755_V1.2290.63
482_T521_A1.2290.63
92_G202_A1.2270.63
574_V578_L1.2240.63
399_L410_F1.2230.62
334_E438_E1.2160.62
503_V574_V1.2110.61
289_D487_T1.2000.60
598_L751_V1.1990.60
20_A70_E1.1890.59
136_D149_D1.1880.59
12_A59_D1.1860.59
504_G512_R1.1830.59
599_L700_A1.1820.59
107_A165_G1.1800.58
44_S61_A1.1800.58
223_I228_V1.1800.58
116_D210_R1.1770.58
56_S217_R1.1740.58
285_L470_T1.1730.58
488_A588_A1.1720.58
130_R181_M1.1720.58
495_R581_L1.1710.57
120_I129_L1.1710.57
499_F581_L1.1680.57
236_P246_L1.1640.57
294_D705_L1.1640.57
518_I568_A1.1550.56
556_A559_E1.1520.56
678_H694_D1.1520.56
113_E214_D1.1510.56
698_A755_V1.1500.55
84_V219_I1.1500.55
136_D147_D1.1480.55
415_G476_T1.1480.55
203_A415_G1.1450.55
523_V585_L1.1440.55
68_L82_V1.1440.55
589_G593_G1.1400.54
260_V276_I1.1390.54
139_D185_R1.1390.54
1_M466_E1.1390.54
438_E476_T1.1370.54
301_L446_F1.1370.54
138_P169_P1.1290.53
510_G564_L1.1290.53
480_E597_K1.1280.53
69_A227_L1.1270.53
20_A74_A1.1260.53
499_F577_L1.1240.53
507_I515_E1.1210.53
600_Q609_V1.1160.52
459_D463_R1.1160.52
24_P28_R1.1120.52
25_Y614_E1.1100.51
746_F751_V1.1040.51
334_E415_G1.1030.51
202_A526_W1.1010.51
257_P468_M1.0980.50
617_D627_R1.0960.50
649_V652_T1.0960.50
510_G560_R1.0920.50
742_V746_F1.0910.50
10_I62_R1.0900.49
185_R189_D1.0900.49
420_K424_D1.0890.49
181_M185_R1.0870.49
515_E564_L1.0850.49
39_L68_L1.0840.49
435_S488_A1.0830.49
531_E535_A1.0830.49
670_R673_L1.0820.49
290_R447_A1.0780.48
83_V257_P1.0760.48
220_G224_A1.0750.48
699_I720_V1.0740.48
722_V727_L1.0730.48
534_E538_L1.0720.48
102_W456_A1.0720.48
28_R639_D1.0710.48
11_S14_F1.0710.48
658_L662_R1.0680.47
319_V426_A1.0660.47
246_L260_V1.0610.47
453_F457_Q1.0590.46
128_R174_D1.0590.46
684_G687_A1.0580.46
360_L414_S1.0570.46
197_A203_A1.0560.46
544_A547_S1.0540.46
213_D252_L1.0530.46
138_P173_H1.0520.46
18_D21_R1.0520.46
320_P359_A1.0510.46
323_K536_Y1.0490.45
491_E705_L1.0450.45
301_L447_A1.0430.45
10_I39_L1.0400.44
151_L160_I1.0390.44
186_A333_S1.0380.44
110_Q493_P1.0350.44
701_L742_V1.0310.44
55_H101_W1.0310.44
337_R405_P1.0300.44
492_A581_L1.0290.43
196_F439_V1.0280.43
54_D57_R1.0270.43
650_D653_G1.0270.43
209_P244_D1.0260.43
205_R208_D1.0260.43
53_V110_Q1.0250.43
492_A495_R1.0240.43
677_Y693_F1.0240.43
225_E467_S1.0230.43
246_L357_A1.0200.43
138_P145_R1.0190.42
698_A727_L1.0180.42
94_A202_A1.0180.42
25_Y638_L1.0170.42
256_R334_E1.0170.42
130_R179_E1.0150.42
92_G102_W1.0140.42
514_L541_A1.0130.42
184_R552_D1.0130.42
683_R721_D1.0120.42
438_E441_G1.0100.42
264_I270_M1.0100.42
517_L536_Y1.0090.41
598_L700_A1.0090.41
453_F691_I1.0090.41
258_I277_A1.0070.41
320_P324_R1.0060.41
73_R76_H1.0060.41
153_Y165_G1.0040.41
223_I231_L1.0040.41
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
1iv8A10.89561000.313Contact Map
3hjeA10.91021000.316Contact Map
1ea9C20.54561000.878Contact Map
1j0hA20.54431000.879Contact Map
4aeeA20.55881000.881Contact Map
2wc7A10.55091000.881Contact Map
2z1kA40.56541000.882Contact Map
1wzlA20.54561000.882Contact Map
1g5aA10.65131000.884Contact Map
4aefA20.54561000.885Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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