| kinase |
| ID: | 1509646968 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 268 (260) | ||
| Sequences: | 58564 (37054.2) | ||
| Seq/Len: | 225.246 | ||
| Nf(neff/√len): | 2298.0 |
HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 225.246).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 241_M | 246_S | 3.551 | 1.00 |
| 6_V | 14_T | 3.179 | 1.00 |
| 133_L | 143_T | 3.175 | 1.00 |
| 107_C | 193_V | 3.085 | 1.00 |
| 17_K | 30_P | 2.921 | 1.00 |
| 5_K | 17_K | 2.855 | 1.00 |
| 115_N | 119_D | 2.657 | 1.00 |
| 238_K | 256_E | 2.569 | 1.00 |
| 6_V | 9_S | 2.479 | 1.00 |
| 132_V | 140_V | 2.379 | 1.00 |
| 83_P | 136_T | 2.366 | 1.00 |
| 115_N | 251_R | 2.347 | 1.00 |
| 111_A | 189_Y | 2.311 | 1.00 |
| 109_Q | 140_V | 2.303 | 1.00 |
| 59_D | 65_R | 2.262 | 1.00 |
| 135_K | 139_H | 2.237 | 1.00 |
| 18_G | 33_I | 2.232 | 1.00 |
| 109_Q | 139_H | 2.157 | 1.00 |
| 110_I | 132_V | 2.110 | 1.00 |
| 18_G | 31_V | 2.052 | 1.00 |
| 20_W | 23_E | 2.021 | 1.00 |
| 243_D | 246_S | 2.004 | 1.00 |
| 186_V | 239_C | 1.994 | 1.00 |
| 88_L | 130_R | 1.972 | 1.00 |
| 60_N | 116_Y | 1.922 | 1.00 |
| 80_Q | 141_K | 1.915 | 1.00 |
| 135_K | 141_K | 1.913 | 1.00 |
| 127_L | 192_T | 1.849 | 1.00 |
| 171_A | 184_S | 1.844 | 1.00 |
| 114_M | 189_Y | 1.836 | 1.00 |
| 114_M | 250_F | 1.782 | 1.00 |
| 114_M | 127_L | 1.771 | 1.00 |
| 17_K | 81_L | 1.724 | 1.00 |
| 236_M | 240_W | 1.722 | 1.00 |
| 6_V | 16_Y | 1.711 | 1.00 |
| 86_C | 89_D | 1.700 | 1.00 |
| 9_S | 14_T | 1.694 | 1.00 |
| 19_L | 30_P | 1.647 | 1.00 |
| 249_K | 252_E | 1.612 | 1.00 |
| 113_G | 142_I | 1.597 | 1.00 |
| 238_K | 248_P | 1.590 | 1.00 |
| 108_V | 112_K | 1.588 | 1.00 |
| 99_G | 102_Y | 1.586 | 1.00 |
| 88_L | 92_R | 1.579 | 1.00 |
| 115_N | 254_I | 1.575 | 1.00 |
| 119_D | 251_R | 1.570 | 1.00 |
| 186_V | 250_F | 1.544 | 1.00 |
| 107_C | 196_L | 1.521 | 1.00 |
| 100_S | 103_L | 1.517 | 1.00 |
| 112_K | 254_I | 1.516 | 1.00 |
| 132_V | 192_T | 1.513 | 1.00 |
| 62_H | 112_K | 1.513 | 1.00 |
| 129_A | 169_W | 1.505 | 1.00 |
| 239_C | 253_L | 1.485 | 1.00 |
| 169_W | 195_E | 1.452 | 1.00 |
| 250_F | 253_L | 1.449 | 1.00 |
| 235_I | 239_C | 1.446 | 1.00 |
| 190_G | 239_C | 1.438 | 1.00 |
| 82_M | 135_K | 1.425 | 1.00 |
| 121_R | 182_H | 1.413 | 1.00 |
| 67_L | 80_Q | 1.413 | 1.00 |
| 107_C | 189_Y | 1.395 | 0.99 |
| 174_S | 184_S | 1.374 | 0.99 |
| 183_Q | 249_K | 1.356 | 0.99 |
| 116_Y | 120_R | 1.354 | 0.99 |
| 105_N | 109_Q | 1.349 | 0.99 |
| 238_K | 241_M | 1.333 | 0.99 |
| 64_C | 82_M | 1.326 | 0.99 |
| 110_I | 140_V | 1.322 | 0.99 |
| 122_L | 148_A | 1.315 | 0.99 |
| 106_W | 140_V | 1.313 | 0.99 |
| 129_A | 192_T | 1.306 | 0.99 |
| 189_Y | 193_V | 1.285 | 0.99 |
| 124_H | 127_L | 1.270 | 0.99 |
| 4_I | 19_L | 1.267 | 0.99 |
| 233_Y | 237_V | 1.263 | 0.99 |
| 191_V | 240_W | 1.235 | 0.98 |
| 112_K | 115_N | 1.232 | 0.98 |
| 103_L | 106_W | 1.232 | 0.98 |
| 125_R | 180_Y | 1.221 | 0.98 |
| 114_M | 118_E | 1.216 | 0.98 |
| 15_V | 32_A | 1.212 | 0.98 |
| 77_L | 80_Q | 1.210 | 0.98 |
| 201_S | 205_D | 1.193 | 0.98 |
| 58_V | 116_Y | 1.193 | 0.98 |
| 65_R | 80_Q | 1.189 | 0.98 |
| 33_I | 78_I | 1.189 | 0.98 |
| 62_H | 113_G | 1.181 | 0.98 |
| 14_T | 37_R | 1.174 | 0.98 |
| 64_C | 143_T | 1.170 | 0.97 |
| 111_A | 253_L | 1.164 | 0.97 |
| 82_M | 141_K | 1.163 | 0.97 |
| 88_L | 129_A | 1.154 | 0.97 |
| 171_A | 174_S | 1.153 | 0.97 |
| 195_E | 202_K | 1.152 | 0.97 |
| 134_V | 140_V | 1.151 | 0.97 |
| 180_Y | 184_S | 1.149 | 0.97 |
| 215_I | 220_E | 1.145 | 0.97 |
| 103_L | 196_L | 1.142 | 0.97 |
| 111_A | 254_I | 1.142 | 0.97 |
| 174_S | 180_Y | 1.139 | 0.97 |
| 129_A | 195_E | 1.137 | 0.97 |
| 110_I | 127_L | 1.135 | 0.97 |
| 108_V | 258_S | 1.133 | 0.97 |
| 244_A | 247_R | 1.124 | 0.97 |
| 126_D | 131_N | 1.123 | 0.97 |
| 248_P | 253_L | 1.120 | 0.96 |
| 224_Q | 227_I | 1.119 | 0.96 |
| 35_E | 76_Q | 1.103 | 0.96 |
| 128_A | 166_P | 1.103 | 0.96 |
| 16_Y | 35_E | 1.100 | 0.96 |
| 88_L | 195_E | 1.088 | 0.96 |
| 84_F | 138_Q | 1.088 | 0.96 |
| 102_Y | 105_N | 1.088 | 0.96 |
| 62_H | 139_H | 1.083 | 0.96 |
| 127_L | 142_I | 1.080 | 0.95 |
| 103_L | 197_M | 1.080 | 0.95 |
| 112_K | 258_S | 1.068 | 0.95 |
| 70_C | 76_Q | 1.063 | 0.95 |
| 189_Y | 250_F | 1.035 | 0.94 |
| 61_P | 139_H | 1.028 | 0.94 |
| 85_G | 93_E | 1.027 | 0.94 |
| 233_Y | 236_M | 1.026 | 0.94 |
| 224_Q | 233_Y | 1.025 | 0.93 |
| 111_A | 250_F | 1.020 | 0.93 |
| 98_I | 106_W | 1.018 | 0.93 |
| 110_I | 192_T | 1.013 | 0.93 |
| 191_V | 195_E | 1.013 | 0.93 |
| 213_S | 216_L | 1.003 | 0.93 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 4otwA | 1 | 0.9664 | 100 | -0.007 | Contact Map |
| 4fl3A | 1 | 0.9366 | 100 | 0.026 | Contact Map |
| 3pozA | 1 | 0.9328 | 100 | 0.026 | Contact Map |
| 4aojA | 3 | 0.9328 | 100 | 0.031 | Contact Map |
| 4rt7A | 1 | 0 | 100 | 0.034 | Contact Map |
| 4aszA | 1 | 0.9739 | 100 | 0.035 | Contact Map |
| 2ozoA | 1 | 0.944 | 100 | 0.039 | Contact Map |
| 4oliA | 1 | 0.944 | 100 | 0.04 | Contact Map |
| 5a46A | 2 | 0 | 100 | 0.041 | Contact Map |
| 4gt4A | 2 | 0.959 | 100 | 0.042 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)