May 4, 2021 - We are working on upgrading the webserver, some pages may not work.
OPENSEQ.org

Hsp104

ID: 1509132411 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 908 (853)
Sequences: 5806 (2697.1)
Seq/Len: 6.807
Nf(neff/√len): 92.3

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 6.807).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
176_E180_Q5.9691.00
173_D242_K5.8631.00
785_D811_K5.6701.00
171_A244_F4.6851.00
348_T376_A4.6561.00
781_H812_D4.5331.00
777_R816_K4.0141.00
843_R848_E3.9711.00
707_R715_T3.8371.00
575_D578_S3.4891.00
345_V376_A3.4871.00
571_H574_R3.4491.00
577_S584_M3.3211.00
273_E309_Q3.3101.00
725_M766_I3.2721.00
807_T858_V3.1861.00
249_A300_I3.1171.00
609_S703_L3.1131.00
199_V338_I2.9521.00
572_M630_F2.9021.00
172_I177_Q2.8891.00
542_D545_T2.8451.00
421_L452_E2.8231.00
788_L806_L2.7951.00
165_E168_S2.7861.00
344_S347_Q2.7791.00
512_D516_Q2.7611.00
789_K792_E2.6951.00
573_E588_K2.6571.00
592_N838_N2.6141.00
776_S779_A2.5971.00
414_L459_L2.5661.00
196_T338_I2.4961.00
494_E506_R2.4921.00
486_L505_L2.4811.00
349_V372_L2.4261.00
483_L510_I2.3771.00
592_N842_L2.3691.00
639_I681_S2.3681.00
367_I400_C2.3531.00
777_R812_D2.3461.00
209_C286_I2.3451.00
579_E629_G2.3421.00
624_A724_I2.3181.00
500_A504_D2.3051.00
428_I449_R2.2981.00
573_E595_R2.2951.00
729_L757_H2.2701.00
164_L168_S2.2631.00
611_L766_I2.2251.00
270_K274_E2.2251.00
236_T239_Q2.2191.00
345_V380_K2.2001.00
176_E179_R2.1851.00
395_L550_A2.1851.00
814_L831_L2.1851.00
429_K432_E2.1841.00
425_Q456_Q2.1721.00
95_V119_A2.1711.00
501_T504_D2.1681.00
817_Y835_E2.1681.00
754_V763_L2.1531.00
563_E571_H2.1361.00
791_I832_I2.1321.00
256_K260_D2.1271.00
251_L268_V2.1261.00
173_D176_E2.1211.00
492_D496_R2.1151.00
475_E516_Q2.1051.00
702_M723_V2.1041.00
286_I325_V2.0721.00
177_Q182_K2.0681.00
587_I623_L2.0131.00
450_Q454_S2.0031.00
649_K694_D1.9981.00
575_D630_F1.9961.00
166_Y267_G1.9841.00
729_L754_V1.9741.00
323_S667_E1.9701.00
581_V786_I1.9701.00
612_F724_I1.9411.00
831_L835_E1.9261.00
373_V377_Q1.9201.00
374_T543_S1.9181.00
702_M708_I1.9151.00
55_I60_Y1.9101.00
579_E622_E1.8991.00
650_Y659_T1.8951.00
509_A512_D1.8891.00
637_M678_K1.8841.00
481_K485_E1.8831.00
650_Y653_S1.8611.00
270_K273_E1.8501.00
579_E626_K1.8431.00
813_F817_Y1.8411.00
613_L727_S1.8311.00
486_L510_I1.8261.00
787_R790_E1.8241.00
809_E858_V1.8161.00
183_L227_Q1.8031.00
290_M331_F1.8031.00
585_D773_N1.8021.00
647_S651_A1.7861.00
375_A396_V1.7751.00
184_D203_R1.7691.00
780_I815_A1.7691.00
345_V377_Q1.7681.00
350_A353_R1.7651.00
782_K785_D1.7561.00
403_V541_V1.7541.00
27_H73_L1.7501.00
379_A388_L1.7291.00
482_K509_A1.7261.00
489_K501_T1.7201.00
570_I845_L1.7171.00
482_K485_E1.7091.00
228_R233_D1.7061.00
166_Y271_E1.7031.00
418_E463_R1.7021.00
734_I746_T1.6961.00
341_A678_K1.6881.00
339_E678_K1.6871.00
571_H575_D1.6821.00
353_R369_D1.6801.00
579_E635_E1.6741.00
227_Q231_D1.6691.00
806_L810_A1.6641.00
773_N779_A1.6601.00
248_L251_L1.6591.00
283_I312_V1.6591.00
485_E489_K1.6561.00
751_M754_V1.6531.00
463_R467_N1.6481.00
585_D747_K1.6421.00
781_H808_Q1.6331.00
807_T856_N1.6311.00
11_A116_I1.6301.00
486_L513_I1.6271.00
37_A66_K1.6261.00
504_D509_A1.6241.00
675_L721_C1.6221.00
399_S549_T1.6131.00
349_V373_V1.6121.00
35_I92_L1.6041.00
577_S587_I1.6031.00
813_F835_E1.5981.00
478_Q482_K1.5881.00
802_Y849_I1.5851.00
229_I243_L1.5801.00
457_E461_P1.5791.00
850_K853_E1.5791.00
498_D501_T1.5731.00
565_E598_R1.5661.00
232_D239_Q1.5641.00
165_E271_E1.5631.00
595_R842_L1.5590.99
787_R829_N1.5580.99
424_I448_A1.5570.99
213_E318_N1.5560.99
453_A457_E1.5450.99
410_K418_E1.5400.99
788_L832_I1.5400.99
95_V120_L1.5280.99
195_S398_I1.5190.99
646_L670_F1.5190.99
370_S543_S1.5080.99
753_A756_Q1.5070.99
357_P361_I1.5020.99
487_E514_K1.4980.99
784_V811_K1.4960.99
576_L580_V1.4960.99
733_F753_A1.4950.99
675_L718_C1.4940.99
751_M771_I1.4940.99
467_N471_H1.4920.99
355_L358_K1.4880.99
420_Q451_K1.4840.99
572_M591_S1.4820.99
843_R846_K1.4790.99
211_I337_K1.4710.99
372_L396_V1.4700.99
490_A510_I1.4570.99
402_G548_E1.4530.99
597_S602_A1.4500.99
781_H811_K1.4480.99
345_V349_V1.4470.99
168_S176_E1.4440.99
639_I675_L1.4420.99
175_T230_I1.4300.99
792_E804_L1.4260.99
457_E460_E1.4260.99
743_Q746_T1.4240.99
503_A506_R1.4230.99
818_G828_L1.4230.99
428_I445_L1.4180.99
479_A513_I1.4150.99
784_V815_A1.4140.99
269_L304_A1.4060.99
807_T810_A1.4060.99
732_E736_S1.3990.99
137_I141_K1.3950.99
699_M702_M1.3950.99
356_Q369_D1.3940.99
652_V698_V1.3940.99
604_P794_R1.3880.99
835_E839_K1.3770.98
785_D808_Q1.3760.98
486_L509_A1.3750.98
21_L38_A1.3700.98
611_L754_V1.3680.98
806_L811_K1.3680.98
272_I281_L1.3670.98
21_L24_D1.3660.98
704_D829_N1.3550.98
817_Y831_L1.3440.98
482_K516_Q1.3440.98
813_F859_L1.3430.98
27_H34_H1.3430.98
589_A770_V1.3410.98
490_A506_R1.3390.98
611_L688_V1.3390.98
348_T388_L1.3280.98
418_E459_L1.3270.98
685_F702_M1.3240.98
25_H74_V1.3210.98
255_A268_V1.3210.98
100_A111_I1.3190.98
843_R849_I1.3190.98
801_H851_D1.3150.98
122_N137_I1.3150.98
240_G278_L1.3130.98
573_E591_S1.3110.98
31_Q89_S1.3100.98
32_P95_V1.3080.98
366_R540_V1.3040.98
483_L513_I1.3000.98
839_K843_R1.2990.98
818_G831_L1.2970.97
653_S656_L1.2960.97
606_Q829_N1.2870.97
489_K492_D1.2830.97
784_V832_I1.2760.97
478_Q481_K1.2720.97
641_V655_L1.2700.97
14_I39_F1.2670.97
347_Q350_A1.2610.97
576_L626_K1.2600.97
265_F268_V1.2590.97
810_A859_L1.2590.97
413_E458_E1.2580.97
812_D816_K1.2560.97
14_I42_T1.2550.97
486_L490_A1.2550.97
88_P92_L1.2550.97
22_A34_H1.2550.97
809_E812_D1.2540.97
762_F766_I1.2540.97
117_L140_I1.2530.97
178_A183_L1.2460.97
399_S546_I1.2450.97
179_R242_K1.2440.97
802_Y844_I1.2390.96
342_E380_K1.2360.96
286_I314_G1.2350.96
840_L855_V1.2330.96
794_R837_L1.2330.96
368_L540_V1.2310.96
647_S694_D1.2300.96
631_L722_I1.2300.96
379_A391_S1.2300.96
28_P79_Q1.2260.96
51_L117_L1.2210.96
404_A408_D1.2180.96
638_M682_V1.2180.96
732_E753_A1.2170.96
656_L698_V1.2130.96
186_V193_I1.2130.96
500_A503_A1.2130.96
58_G135_V1.2120.96
780_I819_Y1.2030.96
814_L818_G1.2020.96
747_K751_M1.1960.95
778_K781_H1.1920.95
103_Q111_I1.1920.95
136_D139_A1.1900.95
836_I857_V1.1890.95
427_E444_R1.1870.95
805_N856_N1.1810.95
570_I846_K1.1800.95
345_V348_T1.1760.95
590_V623_L1.1760.95
368_L542_D1.1740.95
454_S458_E1.1730.95
102_I137_I1.1720.95
596_L838_N1.1700.95
588_K592_N1.1700.95
788_L792_E1.1690.95
177_Q180_Q1.1670.95
803_K854_T1.1670.95
548_E559_K1.1670.95
388_L392_A1.1670.95
432_E449_R1.1640.94
569_L598_R1.1560.94
21_L25_H1.1530.94
649_K654_K1.1480.94
779_A782_K1.1460.94
102_I119_A1.1410.94
578_S789_K1.1400.94
361_I364_G1.1390.94
68_V71_R1.1350.93
658_T685_F1.1340.93
849_I855_V1.1330.93
729_L753_A1.1310.93
173_D244_F1.1300.93
368_L371_A1.1280.93
791_I837_L1.1200.93
777_R815_A1.1200.93
90_Y94_K1.1180.93
420_Q423_L1.1170.93
102_I106_Q1.1140.93
322_R712_Q1.1120.93
743_Q773_N1.1100.92
21_L66_K1.1070.92
785_D789_K1.1060.92
808_Q812_D1.1050.92
98_D101_K1.1020.92
33_I37_A1.1010.92
744_E748_N1.0990.92
32_P92_L1.0980.92
349_V376_A1.0970.92
748_N752_G1.0940.92
10_R13_T1.0920.92
421_L455_L1.0880.91
849_I853_E1.0830.91
460_E463_R1.0790.91
203_R358_K1.0780.91
758_F762_F1.0780.91
7_F11_A1.0770.91
746_T773_N1.0760.91
98_D119_A1.0760.91
564_S567_E1.0750.91
456_Q460_E1.0750.91
832_I836_I1.0730.91
214_P329_G1.0730.91
512_D515_K1.0730.91
545_T548_E1.0720.91
805_N854_T1.0700.91
289_L298_A1.0680.90
751_M755_R1.0660.90
455_L459_L1.0640.90
248_L289_L1.0640.90
483_L517_I1.0640.90
195_S198_R1.0630.90
362_H404_A1.0620.90
376_A380_K1.0610.90
196_T210_L1.0600.90
590_V770_V1.0600.90
353_R373_V1.0590.90
459_L463_R1.0560.90
475_E478_Q1.0560.90
652_V695_V1.0550.90
752_G756_Q1.0540.90
379_A395_L1.0500.89
474_E478_Q1.0500.89
247_D266_K1.0490.89
33_I91_A1.0480.89
792_E806_L1.0470.89
685_F725_M1.0470.89
410_K463_R1.0470.89
647_S697_T1.0460.89
14_I35_I1.0450.89
488_N492_D1.0450.89
272_I279_I1.0440.89
214_P387_R1.0410.89
175_T183_L1.0350.88
622_E626_K1.0340.88
64_L67_K1.0300.88
790_E793_E1.0260.88
15_L96_L1.0250.88
570_I842_L1.0220.88
603_N790_E1.0210.88
513_I516_Q1.0200.87
102_I105_Q1.0200.87
471_H475_E1.0190.87
622_E787_R1.0180.87
374_T377_Q1.0160.87
72_N129_I1.0130.87
641_V654_K1.0120.87
14_I113_Q1.0100.87
468_E471_H1.0090.87
654_K711_G1.0080.87
205_K394_D1.0080.87
421_L459_L1.0060.87
17_L38_A1.0050.86
139_A142_Q1.0050.86
68_V132_E1.0030.86
781_H785_D1.0010.86
665_Y711_G1.0010.86
648_E659_T1.0010.86
349_V377_Q1.0000.86
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
1qvrA30.88221000.445Contact Map
4d2uA60.86671000.448Contact Map
1r6bX10.7611000.473Contact Map
3pxiA30.78411000.5Contact Map
4ciuA10.72251000.572Contact Map
3pxgA60.49121000.745Contact Map
3vkgA20.79851000.768Contact Map
4hseA10.40641000.792Contact Map
4lj9A10.36341000.811Contact Map
4akgA20.79851000.842Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 1.1337 seconds.