| FAT_boundaries |
| ID: | 1508782679 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 660 (659) | ||
| Sequences: | 10308 (8650.3) | ||
| Seq/Len: | 15.642 | ||
| Nf(neff/√len): | 337.0 |
HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 15.642).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 44_L | 61_M | 5.343 | 1.00 |
| 306_H | 344_W | 4.238 | 1.00 |
| 230_L | 269_A | 4.106 | 1.00 |
| 63_E | 239_R | 3.871 | 1.00 |
| 270_R | 278_E | 3.060 | 1.00 |
| 539_T | 552_V | 3.031 | 1.00 |
| 446_R | 449_Q | 2.989 | 1.00 |
| 512_N | 598_K | 2.777 | 1.00 |
| 214_I | 269_A | 2.646 | 1.00 |
| 227_A | 280_L | 2.641 | 1.00 |
| 215_L | 231_F | 2.616 | 1.00 |
| 392_V | 418_D | 2.587 | 1.00 |
| 244_R | 247_L | 2.586 | 1.00 |
| 190_T | 212_I | 2.569 | 1.00 |
| 175_K | 193_E | 2.563 | 1.00 |
| 85_K | 110_A | 2.546 | 1.00 |
| 408_H | 437_Q | 2.493 | 1.00 |
| 405_V | 434_L | 2.489 | 1.00 |
| 127_D | 131_Q | 2.445 | 1.00 |
| 233_V | 262_M | 2.424 | 1.00 |
| 44_L | 239_R | 2.401 | 1.00 |
| 420_H | 454_P | 2.356 | 1.00 |
| 63_E | 66_N | 2.313 | 1.00 |
| 418_D | 457_R | 2.312 | 1.00 |
| 455_T | 458_Q | 2.260 | 1.00 |
| 47_V | 242_L | 2.253 | 1.00 |
| 66_N | 186_T | 2.227 | 1.00 |
| 402_L | 438_L | 2.212 | 1.00 |
| 398_L | 464_T | 2.162 | 1.00 |
| 84_D | 135_T | 2.158 | 1.00 |
| 212_I | 232_I | 2.152 | 1.00 |
| 420_H | 423_E | 2.146 | 1.00 |
| 406_W | 456_I | 2.132 | 1.00 |
| 397_E | 400_Q | 2.122 | 1.00 |
| 527_E | 531_L | 2.114 | 1.00 |
| 328_G | 358_F | 2.100 | 1.00 |
| 395_T | 417_I | 2.099 | 1.00 |
| 289_I | 316_A | 2.091 | 1.00 |
| 331_V | 364_F | 2.083 | 1.00 |
| 330_Y | 358_F | 2.077 | 1.00 |
| 142_G | 163_D | 2.059 | 1.00 |
| 529_L | 625_L | 2.039 | 1.00 |
| 421_F | 426_G | 2.032 | 1.00 |
| 272_L | 278_E | 2.024 | 1.00 |
| 605_S | 610_L | 2.013 | 1.00 |
| 300_E | 314_V | 1.992 | 1.00 |
| 214_I | 277_I | 1.991 | 1.00 |
| 138_Y | 188_M | 1.990 | 1.00 |
| 66_N | 237_L | 1.989 | 1.00 |
| 417_I | 456_I | 1.983 | 1.00 |
| 272_L | 276_N | 1.974 | 1.00 |
| 40_R | 43_D | 1.955 | 1.00 |
| 192_F | 202_P | 1.942 | 1.00 |
| 539_T | 585_A | 1.933 | 1.00 |
| 69_N | 209_R | 1.929 | 1.00 |
| 405_V | 438_L | 1.911 | 1.00 |
| 227_A | 268_R | 1.909 | 1.00 |
| 76_P | 208_D | 1.890 | 1.00 |
| 211_R | 255_H | 1.888 | 1.00 |
| 128_Y | 132_E | 1.887 | 1.00 |
| 115_P | 118_R | 1.881 | 1.00 |
| 416_G | 419_D | 1.877 | 1.00 |
| 529_L | 622_L | 1.860 | 1.00 |
| 211_R | 233_V | 1.860 | 1.00 |
| 449_Q | 462_L | 1.851 | 1.00 |
| 116_S | 119_K | 1.844 | 1.00 |
| 396_D | 417_I | 1.844 | 1.00 |
| 305_N | 348_D | 1.821 | 1.00 |
| 318_E | 324_K | 1.819 | 1.00 |
| 179_G | 188_M | 1.816 | 1.00 |
| 216_D | 220_A | 1.813 | 1.00 |
| 313_T | 364_F | 1.806 | 1.00 |
| 532_R | 621_E | 1.797 | 1.00 |
| 168_I | 171_N | 1.786 | 1.00 |
| 42_D | 63_E | 1.772 | 1.00 |
| 522_I | 622_L | 1.760 | 1.00 |
| 255_H | 260_E | 1.758 | 1.00 |
| 526_L | 590_A | 1.757 | 1.00 |
| 99_L | 188_M | 1.756 | 1.00 |
| 302_F | 345_L | 1.754 | 1.00 |
| 533_H | 621_E | 1.745 | 1.00 |
| 189_V | 232_I | 1.729 | 1.00 |
| 121_D | 124_A | 1.726 | 1.00 |
| 528_Q | 531_L | 1.724 | 1.00 |
| 330_Y | 360_H | 1.718 | 1.00 |
| 138_Y | 179_G | 1.701 | 1.00 |
| 329_Y | 378_P | 1.700 | 1.00 |
| 84_D | 156_R | 1.696 | 1.00 |
| 95_D | 182_P | 1.695 | 1.00 |
| 453_H | 462_L | 1.686 | 1.00 |
| 42_D | 65_H | 1.664 | 1.00 |
| 391_Y | 414_Q | 1.664 | 1.00 |
| 422_L | 451_F | 1.662 | 1.00 |
| 96_G | 138_Y | 1.662 | 1.00 |
| 229_E | 264_R | 1.659 | 1.00 |
| 399_E | 417_I | 1.654 | 1.00 |
| 403_A | 456_I | 1.650 | 1.00 |
| 361_M | 364_F | 1.649 | 1.00 |
| 39_N | 43_D | 1.645 | 1.00 |
| 224_I | 271_F | 1.642 | 1.00 |
| 112_H | 128_Y | 1.641 | 1.00 |
| 523_R | 527_E | 1.639 | 1.00 |
| 392_V | 417_I | 1.638 | 1.00 |
| 581_D | 584_K | 1.634 | 1.00 |
| 112_H | 134_I | 1.632 | 1.00 |
| 192_F | 276_N | 1.629 | 1.00 |
| 223_P | 226_V | 1.627 | 1.00 |
| 138_Y | 141_T | 1.621 | 1.00 |
| 316_A | 324_K | 1.614 | 1.00 |
| 287_V | 296_P | 1.612 | 1.00 |
| 85_K | 112_H | 1.598 | 1.00 |
| 89_Y | 140_P | 1.592 | 1.00 |
| 43_D | 239_R | 1.592 | 1.00 |
| 327_V | 359_V | 1.589 | 1.00 |
| 303_L | 328_G | 1.585 | 1.00 |
| 62_I | 238_G | 1.581 | 1.00 |
| 138_Y | 161_G | 1.576 | 1.00 |
| 90_S | 96_G | 1.572 | 1.00 |
| 409_V | 434_L | 1.568 | 1.00 |
| 285_N | 374_R | 1.567 | 1.00 |
| 401_Q | 404_Q | 1.567 | 1.00 |
| 304_M | 311_L | 1.565 | 1.00 |
| 486_S | 489_E | 1.561 | 1.00 |
| 85_K | 134_I | 1.560 | 1.00 |
| 514_P | 598_K | 1.559 | 1.00 |
| 535_G | 538_S | 1.552 | 1.00 |
| 396_D | 400_Q | 1.552 | 1.00 |
| 212_I | 230_L | 1.550 | 1.00 |
| 540_Y | 609_L | 1.550 | 1.00 |
| 400_Q | 404_Q | 1.549 | 1.00 |
| 587_L | 609_L | 1.546 | 1.00 |
| 99_L | 138_Y | 1.546 | 1.00 |
| 118_R | 124_A | 1.529 | 1.00 |
| 488_V | 491_R | 1.516 | 1.00 |
| 213_L | 255_H | 1.514 | 1.00 |
| 219_L | 231_F | 1.493 | 1.00 |
| 212_I | 269_A | 1.489 | 1.00 |
| 159_I | 179_G | 1.485 | 1.00 |
| 238_G | 252_F | 1.484 | 1.00 |
| 185_C | 232_I | 1.481 | 1.00 |
| 313_T | 372_V | 1.479 | 1.00 |
| 398_L | 401_Q | 1.470 | 1.00 |
| 70_F | 99_L | 1.470 | 1.00 |
| 70_F | 188_M | 1.466 | 1.00 |
| 402_L | 460_K | 1.466 | 1.00 |
| 114_V | 128_Y | 1.463 | 1.00 |
| 314_V | 328_G | 1.462 | 1.00 |
| 434_L | 450_L | 1.461 | 1.00 |
| 84_D | 109_A | 1.453 | 1.00 |
| 491_R | 494_I | 1.451 | 1.00 |
| 100_D | 138_Y | 1.448 | 1.00 |
| 75_R | 107_A | 1.448 | 1.00 |
| 292_Y | 352_Y | 1.440 | 1.00 |
| 342_R | 358_F | 1.436 | 1.00 |
| 245_P | 249_A | 1.434 | 1.00 |
| 247_L | 251_K | 1.433 | 1.00 |
| 318_E | 321_D | 1.432 | 1.00 |
| 329_Y | 380_V | 1.430 | 1.00 |
| 396_D | 464_T | 1.429 | 1.00 |
| 268_R | 283_L | 1.428 | 1.00 |
| 403_A | 417_I | 1.422 | 1.00 |
| 60_V | 238_G | 1.418 | 1.00 |
| 255_H | 262_M | 1.413 | 1.00 |
| 491_R | 495_I | 1.410 | 1.00 |
| 96_G | 188_M | 1.397 | 0.99 |
| 213_L | 257_Q | 1.386 | 0.99 |
| 6_N | 9_H | 1.379 | 0.99 |
| 302_F | 348_D | 1.378 | 0.99 |
| 395_T | 399_E | 1.372 | 0.99 |
| 485_T | 489_E | 1.372 | 0.99 |
| 214_I | 230_L | 1.369 | 0.99 |
| 87_L | 129_C | 1.368 | 0.99 |
| 48_M | 183_T | 1.365 | 0.99 |
| 427_D | 430_K | 1.353 | 0.99 |
| 87_L | 114_V | 1.345 | 0.99 |
| 185_C | 189_V | 1.343 | 0.99 |
| 392_V | 457_R | 1.337 | 0.99 |
| 395_T | 457_R | 1.336 | 0.99 |
| 641_F | 644_W | 1.332 | 0.99 |
| 510_T | 602_W | 1.330 | 0.99 |
| 518_D | 626_L | 1.329 | 0.99 |
| 161_G | 188_M | 1.326 | 0.99 |
| 518_D | 521_Q | 1.326 | 0.99 |
| 88_V | 113_I | 1.323 | 0.99 |
| 402_L | 456_I | 1.315 | 0.99 |
| 302_F | 305_N | 1.315 | 0.99 |
| 104_H | 109_A | 1.301 | 0.99 |
| 497_Q | 542_M | 1.301 | 0.99 |
| 253_I | 262_M | 1.299 | 0.99 |
| 70_F | 186_T | 1.299 | 0.99 |
| 304_M | 312_T | 1.295 | 0.99 |
| 522_I | 601_L | 1.292 | 0.99 |
| 417_I | 457_R | 1.285 | 0.99 |
| 448_D | 452_T | 1.280 | 0.99 |
| 84_D | 136_I | 1.280 | 0.99 |
| 519_T | 592_V | 1.279 | 0.99 |
| 144_A | 160_T | 1.274 | 0.99 |
| 203_I | 279_F | 1.271 | 0.99 |
| 231_F | 264_R | 1.270 | 0.99 |
| 391_Y | 416_G | 1.261 | 0.99 |
| 433_Q | 437_Q | 1.260 | 0.99 |
| 286_L | 295_E | 1.256 | 0.99 |
| 449_Q | 452_T | 1.256 | 0.99 |
| 141_T | 144_A | 1.256 | 0.99 |
| 344_W | 348_D | 1.254 | 0.99 |
| 62_I | 186_T | 1.248 | 0.99 |
| 403_A | 415_M | 1.245 | 0.98 |
| 510_T | 600_V | 1.242 | 0.98 |
| 205_K | 276_N | 1.240 | 0.98 |
| 165_L | 178_N | 1.230 | 0.98 |
| 46_Y | 62_I | 1.228 | 0.98 |
| 96_G | 99_L | 1.227 | 0.98 |
| 88_V | 100_D | 1.227 | 0.98 |
| 5_T | 9_H | 1.227 | 0.98 |
| 70_F | 100_D | 1.221 | 0.98 |
| 590_A | 603_F | 1.220 | 0.98 |
| 136_I | 157_V | 1.218 | 0.98 |
| 429_I | 432_M | 1.217 | 0.98 |
| 337_P | 340_E | 1.217 | 0.98 |
| 485_T | 547_I | 1.214 | 0.98 |
| 512_N | 600_V | 1.211 | 0.98 |
| 269_A | 279_F | 1.208 | 0.98 |
| 342_R | 346_G | 1.206 | 0.98 |
| 71_C | 103_T | 1.205 | 0.98 |
| 289_I | 324_K | 1.205 | 0.98 |
| 401_Q | 441_I | 1.205 | 0.98 |
| 534_E | 637_T | 1.204 | 0.98 |
| 144_A | 158_V | 1.201 | 0.98 |
| 138_Y | 143_A | 1.195 | 0.98 |
| 332_A | 360_H | 1.194 | 0.98 |
| 593_I | 602_W | 1.191 | 0.98 |
| 157_V | 177_Y | 1.188 | 0.98 |
| 104_H | 111_L | 1.188 | 0.98 |
| 484_E | 489_E | 1.188 | 0.98 |
| 521_Q | 626_L | 1.187 | 0.98 |
| 81_T | 84_D | 1.185 | 0.98 |
| 398_L | 463_L | 1.178 | 0.98 |
| 207_I | 210_T | 1.176 | 0.98 |
| 99_L | 179_G | 1.175 | 0.98 |
| 310_E | 333_P | 1.174 | 0.98 |
| 435_I | 450_L | 1.174 | 0.98 |
| 528_Q | 625_L | 1.172 | 0.98 |
| 87_L | 134_I | 1.167 | 0.97 |
| 231_F | 253_I | 1.163 | 0.97 |
| 434_L | 459_L | 1.160 | 0.97 |
| 285_N | 300_E | 1.155 | 0.97 |
| 100_D | 188_M | 1.154 | 0.97 |
| 318_E | 322_G | 1.153 | 0.97 |
| 590_A | 601_L | 1.143 | 0.97 |
| 605_S | 609_L | 1.142 | 0.97 |
| 71_C | 106_L | 1.141 | 0.97 |
| 70_F | 138_Y | 1.140 | 0.97 |
| 227_A | 283_L | 1.136 | 0.97 |
| 35_L | 39_N | 1.128 | 0.97 |
| 124_A | 127_D | 1.126 | 0.97 |
| 489_E | 492_M | 1.120 | 0.96 |
| 202_P | 276_N | 1.120 | 0.96 |
| 89_Y | 188_M | 1.118 | 0.96 |
| 399_E | 460_K | 1.118 | 0.96 |
| 270_R | 280_L | 1.115 | 0.96 |
| 438_L | 463_L | 1.110 | 0.96 |
| 214_I | 222_Q | 1.109 | 0.96 |
| 80_V | 109_A | 1.107 | 0.96 |
| 80_V | 84_D | 1.105 | 0.96 |
| 592_V | 599_K | 1.105 | 0.96 |
| 89_Y | 139_L | 1.104 | 0.96 |
| 541_H | 550_R | 1.101 | 0.96 |
| 88_V | 111_L | 1.099 | 0.96 |
| 216_D | 222_Q | 1.096 | 0.96 |
| 297_G | 300_E | 1.096 | 0.96 |
| 373_D | 376_A | 1.093 | 0.96 |
| 406_W | 426_G | 1.091 | 0.96 |
| 89_Y | 143_A | 1.091 | 0.96 |
| 90_S | 138_Y | 1.091 | 0.96 |
| 31_N | 35_L | 1.090 | 0.96 |
| 74_Y | 99_L | 1.086 | 0.96 |
| 129_C | 133_G | 1.083 | 0.96 |
| 211_R | 260_E | 1.083 | 0.96 |
| 637_T | 640_S | 1.080 | 0.95 |
| 296_P | 314_V | 1.078 | 0.95 |
| 231_F | 262_M | 1.078 | 0.95 |
| 87_L | 128_Y | 1.077 | 0.95 |
| 538_S | 549_K | 1.077 | 0.95 |
| 139_L | 144_A | 1.075 | 0.95 |
| 592_V | 601_L | 1.073 | 0.95 |
| 96_G | 161_G | 1.073 | 0.95 |
| 406_W | 459_L | 1.072 | 0.95 |
| 588_L | 603_F | 1.070 | 0.95 |
| 507_V | 610_L | 1.069 | 0.95 |
| 338_H | 358_F | 1.069 | 0.95 |
| 157_V | 175_K | 1.068 | 0.95 |
| 431_V | 447_Y | 1.068 | 0.95 |
| 394_P | 417_I | 1.067 | 0.95 |
| 594_E | 599_K | 1.067 | 0.95 |
| 61_M | 242_L | 1.066 | 0.95 |
| 344_W | 347_N | 1.065 | 0.95 |
| 575_E | 604_D | 1.065 | 0.95 |
| 517_V | 599_K | 1.062 | 0.95 |
| 306_H | 341_L | 1.060 | 0.95 |
| 401_Q | 440_N | 1.058 | 0.95 |
| 177_Y | 193_E | 1.058 | 0.95 |
| 412_I | 415_M | 1.056 | 0.95 |
| 70_F | 187_I | 1.055 | 0.95 |
| 396_D | 399_E | 1.053 | 0.95 |
| 492_M | 495_I | 1.051 | 0.94 |
| 233_V | 260_E | 1.050 | 0.94 |
| 65_H | 235_E | 1.047 | 0.94 |
| 579_P | 589_R | 1.047 | 0.94 |
| 638_Y | 641_F | 1.045 | 0.94 |
| 69_N | 235_E | 1.039 | 0.94 |
| 179_G | 191_M | 1.038 | 0.94 |
| 391_Y | 394_P | 1.034 | 0.94 |
| 330_Y | 341_L | 1.034 | 0.94 |
| 49_Y | 59_G | 1.033 | 0.94 |
| 655_E | 659_A | 1.033 | 0.94 |
| 517_V | 522_I | 1.032 | 0.94 |
| 582_L | 608_I | 1.031 | 0.94 |
| 86_A | 104_H | 1.031 | 0.94 |
| 534_E | 537_R | 1.028 | 0.94 |
| 114_V | 125_L | 1.028 | 0.94 |
| 75_R | 103_T | 1.027 | 0.94 |
| 329_Y | 377_L | 1.027 | 0.94 |
| 493_Y | 497_Q | 1.027 | 0.94 |
| 538_S | 551_I | 1.026 | 0.94 |
| 97_S | 101_I | 1.023 | 0.93 |
| 213_L | 233_V | 1.023 | 0.93 |
| 165_L | 176_L | 1.022 | 0.93 |
| 522_I | 626_L | 1.021 | 0.93 |
| 129_C | 137_S | 1.021 | 0.93 |
| 443_L | 463_L | 1.021 | 0.93 |
| 317_K | 379_D | 1.017 | 0.93 |
| 215_L | 262_M | 1.015 | 0.93 |
| 403_A | 407_S | 1.014 | 0.93 |
| 98_A | 187_I | 1.014 | 0.93 |
| 487_A | 645_Q | 1.014 | 0.93 |
| 89_Y | 114_V | 1.011 | 0.93 |
| 185_C | 237_L | 1.007 | 0.93 |
| 453_H | 458_Q | 1.007 | 0.93 |
| 482_E | 548_V | 1.006 | 0.93 |
| 232_I | 265_T | 1.005 | 0.93 |
| 289_I | 294_I | 1.003 | 0.93 |
| 563_T | 594_E | 1.002 | 0.92 |
| 543_R | 546_E | 1.002 | 0.92 |
| 450_L | 459_L | 1.002 | 0.92 |
| 620_R | 624_A | 1.000 | 0.92 |
| 231_F | 251_K | 1.000 | 0.92 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 2vsqA | 1 | 0.8773 | 100 | 0.338 | Contact Map |
| 4dg8A | 1 | 0.5621 | 100 | 0.363 | Contact Map |
| 4iz6A | 2 | 0.6924 | 100 | 0.39 | Contact Map |
| 4r0mB | 2 | 0.6864 | 100 | 0.406 | Contact Map |
| 1amuA | 2 | 0.5758 | 100 | 0.477 | Contact Map |
| 4d4iA | 1 | 0 | 100 | 0.48 | Contact Map |
| 3g7sA | 2 | 0.5152 | 100 | 0.521 | Contact Map |
| 3wvnA | 2 | 0.3985 | 100 | 0.522 | Contact Map |
| 3vnrA | 1 | 0.5561 | 100 | 0.526 | Contact Map |
| 4gr5A | 4 | 0.4182 | 100 | 0.527 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)