| UNI |
| ID: | 1508096274 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 435 (415) | ||
| Sequences: | 975 (583.9) | ||
| Seq/Len: | 2.349 | ||
| Nf(neff/√len): | 28.7 |
HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 2.349).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 392_Y | 414_S | 4.458 | 1.00 |
| 407_L | 411_S | 3.045 | 1.00 |
| 291_W | 295_R | 2.910 | 1.00 |
| 20_F | 264_S | 2.662 | 1.00 |
| 63_V | 170_L | 2.635 | 1.00 |
| 167_S | 186_F | 2.634 | 1.00 |
| 289_D | 305_K | 2.332 | 1.00 |
| 95_T | 340_L | 2.327 | 1.00 |
| 62_L | 93_W | 2.169 | 1.00 |
| 300_S | 304_R | 2.093 | 1.00 |
| 252_K | 256_Y | 2.043 | 0.99 |
| 237_Q | 241_I | 2.035 | 0.99 |
| 273_P | 276_G | 2.023 | 0.99 |
| 183_F | 187_Q | 1.980 | 0.99 |
| 350_C | 414_S | 1.953 | 0.99 |
| 184_Q | 188_A | 1.885 | 0.99 |
| 347_G | 351_W | 1.829 | 0.98 |
| 139_E | 321_H | 1.774 | 0.98 |
| 20_F | 23_A | 1.762 | 0.98 |
| 401_S | 404_S | 1.718 | 0.97 |
| 275_L | 282_E | 1.665 | 0.97 |
| 411_S | 414_S | 1.644 | 0.96 |
| 375_R | 379_R | 1.642 | 0.96 |
| 383_W | 387_Q | 1.640 | 0.96 |
| 86_T | 134_S | 1.615 | 0.96 |
| 124_M | 334_S | 1.613 | 0.96 |
| 274_E | 283_G | 1.588 | 0.95 |
| 98_H | 124_M | 1.572 | 0.95 |
| 335_A | 338_H | 1.524 | 0.94 |
| 124_M | 306_W | 1.524 | 0.94 |
| 149_F | 152_R | 1.517 | 0.94 |
| 274_E | 281_E | 1.514 | 0.94 |
| 132_S | 317_L | 1.506 | 0.93 |
| 132_S | 180_L | 1.488 | 0.93 |
| 306_W | 334_S | 1.486 | 0.93 |
| 289_D | 292_T | 1.484 | 0.93 |
| 256_Y | 334_S | 1.483 | 0.93 |
| 61_V | 65_T | 1.462 | 0.92 |
| 273_P | 282_E | 1.461 | 0.92 |
| 306_W | 314_L | 1.448 | 0.92 |
| 235_C | 239_E | 1.446 | 0.91 |
| 265_L | 271_F | 1.445 | 0.91 |
| 322_S | 329_Q | 1.411 | 0.90 |
| 129_R | 182_S | 1.406 | 0.90 |
| 23_A | 172_F | 1.398 | 0.90 |
| 319_F | 345_V | 1.390 | 0.89 |
| 263_D | 267_H | 1.388 | 0.89 |
| 248_A | 254_T | 1.383 | 0.89 |
| 70_A | 73_L | 1.383 | 0.89 |
| 12_I | 16_Q | 1.368 | 0.88 |
| 171_F | 174_A | 1.350 | 0.87 |
| 94_Q | 97_C | 1.344 | 0.87 |
| 347_G | 396_A | 1.335 | 0.86 |
| 128_Q | 382_T | 1.325 | 0.86 |
| 183_F | 252_K | 1.317 | 0.85 |
| 35_F | 39_A | 1.310 | 0.85 |
| 302_F | 390_I | 1.309 | 0.85 |
| 39_A | 186_F | 1.307 | 0.85 |
| 360_L | 364_F | 1.306 | 0.85 |
| 261_L | 340_L | 1.303 | 0.85 |
| 362_H | 378_Y | 1.297 | 0.84 |
| 204_A | 207_W | 1.295 | 0.84 |
| 314_L | 334_S | 1.292 | 0.84 |
| 268_A | 311_A | 1.291 | 0.84 |
| 95_T | 341_H | 1.286 | 0.84 |
| 248_A | 394_M | 1.283 | 0.83 |
| 298_R | 362_H | 1.280 | 0.83 |
| 239_E | 243_V | 1.266 | 0.82 |
| 314_L | 330_T | 1.266 | 0.82 |
| 98_H | 352_A | 1.265 | 0.82 |
| 123_L | 130_V | 1.259 | 0.82 |
| 350_C | 393_I | 1.251 | 0.81 |
| 160_C | 321_H | 1.249 | 0.81 |
| 362_H | 379_R | 1.244 | 0.81 |
| 136_D | 182_S | 1.244 | 0.81 |
| 230_A | 381_L | 1.243 | 0.81 |
| 252_K | 294_E | 1.241 | 0.80 |
| 328_L | 352_A | 1.238 | 0.80 |
| 251_F | 398_E | 1.235 | 0.80 |
| 24_L | 268_A | 1.229 | 0.80 |
| 213_L | 418_M | 1.217 | 0.79 |
| 48_G | 51_L | 1.211 | 0.78 |
| 139_E | 160_C | 1.202 | 0.77 |
| 90_Q | 130_V | 1.200 | 0.77 |
| 63_V | 315_R | 1.199 | 0.77 |
| 11_P | 14_F | 1.199 | 0.77 |
| 255_Y | 259_W | 1.199 | 0.77 |
| 311_A | 326_P | 1.198 | 0.77 |
| 208_R | 212_I | 1.192 | 0.77 |
| 91_M | 310_T | 1.190 | 0.76 |
| 274_E | 282_E | 1.188 | 0.76 |
| 160_C | 344_Q | 1.187 | 0.76 |
| 410_N | 415_V | 1.186 | 0.76 |
| 21_P | 24_L | 1.180 | 0.76 |
| 43_F | 47_G | 1.180 | 0.76 |
| 205_L | 212_I | 1.179 | 0.75 |
| 91_M | 333_F | 1.175 | 0.75 |
| 124_M | 256_Y | 1.172 | 0.75 |
| 256_Y | 306_W | 1.172 | 0.75 |
| 315_R | 320_Q | 1.168 | 0.74 |
| 256_Y | 294_E | 1.162 | 0.74 |
| 307_N | 310_T | 1.158 | 0.74 |
| 301_V | 305_K | 1.157 | 0.74 |
| 344_Q | 348_F | 1.156 | 0.73 |
| 331_F | 334_S | 1.155 | 0.73 |
| 154_S | 182_S | 1.153 | 0.73 |
| 97_C | 123_L | 1.151 | 0.73 |
| 124_M | 314_L | 1.148 | 0.73 |
| 206_S | 209_G | 1.142 | 0.72 |
| 161_K | 164_P | 1.141 | 0.72 |
| 314_L | 362_H | 1.140 | 0.72 |
| 164_P | 187_Q | 1.139 | 0.72 |
| 35_F | 186_F | 1.138 | 0.72 |
| 54_A | 57_G | 1.137 | 0.72 |
| 111_P | 298_R | 1.135 | 0.71 |
| 346_F | 406_W | 1.129 | 0.71 |
| 34_S | 423_L | 1.127 | 0.71 |
| 295_R | 385_H | 1.124 | 0.70 |
| 318_V | 333_F | 1.118 | 0.70 |
| 111_P | 255_Y | 1.116 | 0.70 |
| 307_N | 313_W | 1.112 | 0.69 |
| 234_D | 380_T | 1.109 | 0.69 |
| 244_V | 398_E | 1.104 | 0.68 |
| 236_Q | 239_E | 1.102 | 0.68 |
| 156_S | 160_C | 1.099 | 0.68 |
| 266_L | 274_E | 1.099 | 0.68 |
| 73_L | 133_L | 1.095 | 0.68 |
| 259_W | 348_F | 1.094 | 0.67 |
| 151_S | 155_L | 1.091 | 0.67 |
| 357_A | 393_I | 1.090 | 0.67 |
| 124_M | 331_F | 1.090 | 0.67 |
| 185_R | 188_A | 1.087 | 0.67 |
| 88_C | 399_V | 1.086 | 0.67 |
| 307_N | 339_G | 1.085 | 0.66 |
| 64_F | 177_G | 1.081 | 0.66 |
| 129_R | 305_K | 1.079 | 0.66 |
| 98_H | 306_W | 1.079 | 0.66 |
| 128_Q | 331_F | 1.077 | 0.66 |
| 103_Y | 341_H | 1.075 | 0.65 |
| 252_K | 334_S | 1.075 | 0.65 |
| 389_I | 417_P | 1.072 | 0.65 |
| 56_M | 240_C | 1.070 | 0.65 |
| 298_R | 358_D | 1.064 | 0.64 |
| 14_F | 17_G | 1.059 | 0.64 |
| 342_P | 402_L | 1.057 | 0.64 |
| 339_G | 348_F | 1.056 | 0.63 |
| 303_S | 347_G | 1.053 | 0.63 |
| 221_A | 231_G | 1.052 | 0.63 |
| 408_L | 411_S | 1.051 | 0.63 |
| 366_N | 379_R | 1.050 | 0.63 |
| 186_F | 190_V | 1.049 | 0.63 |
| 98_H | 255_Y | 1.048 | 0.63 |
| 183_F | 190_V | 1.048 | 0.63 |
| 176_L | 365_A | 1.047 | 0.62 |
| 339_G | 343_G | 1.045 | 0.62 |
| 252_K | 306_W | 1.042 | 0.62 |
| 96_L | 353_V | 1.041 | 0.62 |
| 340_L | 345_V | 1.040 | 0.62 |
| 98_H | 315_R | 1.039 | 0.62 |
| 37_T | 41_Y | 1.037 | 0.61 |
| 48_G | 57_G | 1.037 | 0.61 |
| 12_I | 18_A | 1.037 | 0.61 |
| 167_S | 353_V | 1.036 | 0.61 |
| 173_P | 331_F | 1.035 | 0.61 |
| 310_T | 315_R | 1.031 | 0.61 |
| 25_L | 29_L | 1.030 | 0.61 |
| 140_G | 321_H | 1.028 | 0.60 |
| 307_N | 348_F | 1.027 | 0.60 |
| 95_T | 336_W | 1.026 | 0.60 |
| 125_L | 311_A | 1.026 | 0.60 |
| 182_S | 185_R | 1.025 | 0.60 |
| 187_Q | 191_Q | 1.024 | 0.60 |
| 410_N | 414_S | 1.021 | 0.60 |
| 166_F | 177_G | 1.020 | 0.59 |
| 110_E | 124_M | 1.019 | 0.59 |
| 67_A | 71_V | 1.018 | 0.59 |
| 306_W | 351_W | 1.015 | 0.59 |
| 87_F | 91_M | 1.013 | 0.59 |
| 339_G | 344_Q | 1.012 | 0.59 |
| 188_A | 274_E | 1.011 | 0.58 |
| 169_L | 361_I | 1.007 | 0.58 |
| 87_F | 255_Y | 1.007 | 0.58 |
| 294_E | 307_N | 1.007 | 0.58 |
| 188_A | 236_Q | 1.005 | 0.58 |
| 128_Q | 317_L | 1.000 | 0.57 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 1q90L | 1 | 0.0736 | 1.6 | 0.985 | Contact Map |
| 1irqA | 2 | 0.1126 | 1.6 | 0.985 | Contact Map |
| 2knuA | 1 | 0.0506 | 1.5 | 0.985 | Contact Map |
| 4ogqE | 1 | 0.0713 | 1.4 | 0.986 | Contact Map |
| 2vdaB | 1 | 0.0575 | 1.4 | 0.986 | Contact Map |
| 3pjzA | 2 | 0.1425 | 1.3 | 0.986 | Contact Map |
| 3jysA | 1 | 0.1379 | 1.1 | 0.986 | Contact Map |
| 2le3A | 1 | 0.0966 | 1.1 | 0.987 | Contact Map |
| 1rmkA | 1 | 0.0713 | 1 | 0.987 | Contact Map |
| 2ls4A | 1 | 0.0552 | 0.9 | 0.987 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)