| TMEM97 |
| ID: | 1504038477 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 176 (172) | ||
| Sequences: | 398 (318.2) | ||
| Seq/Len: | 2.314 | ||
| Nf(neff/√len): | 24.3 |
HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 2.314).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 22_I | 78_L | 2.438 | 1.00 |
| 50_Y | 54_F | 2.096 | 1.00 |
| 80_F | 99_P | 1.976 | 0.99 |
| 104_A | 158_L | 1.902 | 0.99 |
| 30_L | 74_L | 1.900 | 0.99 |
| 72_C | 106_H | 1.861 | 0.99 |
| 21_H | 81_F | 1.785 | 0.98 |
| 157_L | 160_F | 1.729 | 0.97 |
| 55_K | 139_E | 1.720 | 0.97 |
| 77_Q | 81_F | 1.713 | 0.97 |
| 10_V | 13_L | 1.713 | 0.97 |
| 111_L | 114_I | 1.663 | 0.97 |
| 14_L | 160_F | 1.629 | 0.96 |
| 170_E | 174_K | 1.599 | 0.95 |
| 17_Y | 21_H | 1.560 | 0.95 |
| 25_T | 78_L | 1.543 | 0.94 |
| 30_L | 110_T | 1.522 | 0.94 |
| 22_I | 82_P | 1.517 | 0.94 |
| 15_G | 85_A | 1.513 | 0.93 |
| 108_I | 154_P | 1.499 | 0.93 |
| 31_Q | 40_P | 1.493 | 0.93 |
| 160_F | 164_N | 1.480 | 0.92 |
| 19_V | 85_A | 1.471 | 0.92 |
| 19_V | 22_I | 1.468 | 0.92 |
| 73_E | 77_Q | 1.454 | 0.92 |
| 143_L | 147_Y | 1.423 | 0.90 |
| 71_F | 75_V | 1.419 | 0.90 |
| 5_T | 9_C | 1.409 | 0.90 |
| 50_Y | 56_D | 1.406 | 0.90 |
| 26_M | 78_L | 1.395 | 0.89 |
| 21_H | 25_T | 1.368 | 0.88 |
| 31_Q | 34_L | 1.344 | 0.87 |
| 101_I | 156_I | 1.344 | 0.87 |
| 32_A | 158_L | 1.333 | 0.86 |
| 18_F | 103_Y | 1.332 | 0.86 |
| 166_Y | 170_E | 1.316 | 0.85 |
| 86_Y | 90_K | 1.314 | 0.85 |
| 81_F | 154_P | 1.284 | 0.83 |
| 25_T | 81_F | 1.271 | 0.82 |
| 58_L | 109_T | 1.245 | 0.80 |
| 83_I | 153_I | 1.235 | 0.80 |
| 108_I | 158_L | 1.221 | 0.79 |
| 7_R | 12_W | 1.221 | 0.79 |
| 128_A | 132_Q | 1.216 | 0.78 |
| 4_V | 8_R | 1.215 | 0.78 |
| 49_W | 53_E | 1.189 | 0.76 |
| 88_F | 95_W | 1.184 | 0.75 |
| 21_H | 24_I | 1.168 | 0.74 |
| 58_L | 66_F | 1.163 | 0.74 |
| 50_Y | 73_E | 1.163 | 0.74 |
| 10_V | 20_S | 1.160 | 0.73 |
| 77_Q | 154_P | 1.158 | 0.73 |
| 57_P | 154_P | 1.135 | 0.71 |
| 133_R | 136_N | 1.135 | 0.71 |
| 80_F | 84_A | 1.127 | 0.70 |
| 100_A | 165_P | 1.125 | 0.70 |
| 14_L | 157_L | 1.124 | 0.70 |
| 29_D | 35_P | 1.123 | 0.70 |
| 143_L | 150_Y | 1.121 | 0.70 |
| 77_Q | 147_Y | 1.119 | 0.69 |
| 2_G | 5_T | 1.116 | 0.69 |
| 49_W | 52_K | 1.116 | 0.69 |
| 74_L | 108_I | 1.114 | 0.69 |
| 40_P | 119_L | 1.112 | 0.69 |
| 71_F | 156_I | 1.107 | 0.68 |
| 46_L | 49_W | 1.105 | 0.68 |
| 8_R | 12_W | 1.105 | 0.68 |
| 17_Y | 81_F | 1.104 | 0.68 |
| 65_W | 115_L | 1.096 | 0.67 |
| 98_I | 159_L | 1.094 | 0.67 |
| 114_I | 164_N | 1.092 | 0.67 |
| 76_F | 107_T | 1.088 | 0.66 |
| 5_T | 8_R | 1.084 | 0.66 |
| 100_A | 104_A | 1.079 | 0.65 |
| 82_P | 98_I | 1.065 | 0.64 |
| 32_A | 47_L | 1.059 | 0.63 |
| 33_L | 70_L | 1.056 | 0.63 |
| 106_H | 113_P | 1.048 | 0.62 |
| 5_T | 11_E | 1.035 | 0.61 |
| 171_E | 174_K | 1.031 | 0.60 |
| 171_E | 175_K | 1.026 | 0.60 |
| 77_Q | 103_Y | 1.023 | 0.59 |
| 21_H | 77_Q | 1.020 | 0.59 |
| 69_F | 113_P | 1.020 | 0.59 |
| 145_G | 151_L | 1.017 | 0.59 |
| 8_R | 11_E | 1.015 | 0.58 |
| 108_I | 115_L | 1.015 | 0.58 |
| 38_L | 64_V | 1.007 | 0.57 |
| 84_A | 96_I | 1.004 | 0.57 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 2mheA | 2 | 0.1761 | 1.8 | 0.963 | Contact Map |
| 3wvfA | 2 | 0.5284 | 1.4 | 0.965 | Contact Map |
| 5a63D | 1 | 0 | 1.4 | 0.966 | Contact Map |
| 2wscI | 1 | 0.1364 | 1.2 | 0.967 | Contact Map |
| 2ka4B | 1 | 0.2557 | 1.2 | 0.967 | Contact Map |
| 2qjyA | 6 | 0.3864 | 1.1 | 0.968 | Contact Map |
| 3wajA | 1 | 0.6989 | 1.1 | 0.968 | Contact Map |
| 1hznA | 1 | 0.1648 | 0.9 | 0.969 | Contact Map |
| 4l6vi | 3 | 0.1932 | 0.9 | 0.97 | Contact Map |
| 4f3fC | 1 | 0.0739 | 0.9 | 0.97 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)