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OPENSEQ.org

UCH37

ID: 1503020968 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 329 (323)
Sequences: 530 (348.2)
Seq/Len: 1.641
Nf(neff/√len): 19.4

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 1.641).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
174_R187_D3.5001.00
123_A126_K2.5251.00
77_I171_V2.3081.00
94_V130_L2.1080.99
249_D253_G2.1010.99
12_E15_P2.0980.99
122_D125_M1.9110.98
22_I97_L1.8820.97
314_E318_E1.8760.97
118_S121_F1.8340.97
287_R294_P1.8240.97
114_F118_S1.7050.95
28_R106_H1.7040.95
15_P196_W1.6980.94
95_S228_K1.6820.94
274_E277_K1.6620.94
322_A326_Q1.6500.93
114_F167_S1.6240.92
8_W12_E1.5960.92
177_E207_R1.5740.91
200_V204_I1.5180.89
126_K129_A1.5050.88
227_R230_I1.4980.88
11_M51_G1.4800.87
109_E113_E1.4680.86
273_E276_Q1.4670.86
91_Q95_S1.4650.86
110_T166_V1.4640.86
16_G19_T1.4610.86
112_S227_R1.4570.86
287_R292_Y1.4470.85
298_E301_K1.4370.85
30_A50_H1.4200.84
20_E285_N1.4060.83
118_S129_A1.4050.83
263_E266_K1.3850.82
50_H225_S1.3780.81
235_I264_V1.3770.81
22_I93_I1.3770.81
51_G97_L1.3630.80
22_I32_V1.3590.80
31_Q48_P1.3530.80
245_E253_G1.3420.79
174_R190_A1.3300.78
166_V322_A1.3220.77
226_D278_L1.3090.76
78_F285_N1.3080.76
321_N325_A1.3000.76
78_F95_S1.2970.75
106_H112_S1.2940.75
93_I184_G1.2900.75
175_L199_A1.2860.75
292_Y312_L1.2830.74
117_F121_F1.2740.74
46_L211_Y1.2620.73
235_I267_N1.2600.72
170_P274_E1.2580.72
228_K275_V1.2570.72
126_K227_R1.2570.72
16_G31_Q1.2520.72
110_T113_E1.2500.72
11_M17_V1.2490.71
182_R186_I1.2390.71
84_I117_F1.2380.70
52_L58_W1.2320.70
246_E255_S1.2270.70
121_F224_V1.2270.70
52_L197_I1.2260.69
58_W182_R1.2170.69
76_T172_N1.2170.69
39_E197_I1.2130.68
8_W13_S1.2090.68
11_M20_E1.2080.68
139_V143_F1.2070.68
284_E290_H1.2030.67
177_E182_R1.2020.67
234_K263_E1.1960.67
49_V221_M1.1950.67
24_G139_V1.1900.66
114_F130_L1.1900.66
16_G78_F1.1890.66
16_G95_S1.1710.64
316_A319_K1.1660.64
228_K278_L1.1620.63
204_I219_N1.1580.63
81_K123_A1.1570.63
316_A323_K1.1560.63
19_T33_E1.1470.62
84_I132_N1.1460.62
121_F126_K1.1440.62
320_Q324_K1.1440.62
221_M276_Q1.1370.61
157_A160_E1.1370.61
170_P196_W1.1340.61
20_E24_G1.1310.60
9_C25_F1.1310.60
216_I224_V1.1220.59
56_F211_Y1.1150.59
47_K195_D1.1140.59
321_N324_K1.1130.58
19_T123_A1.1110.58
175_L191_C1.1090.58
50_H205_E1.1070.58
93_I121_F1.1070.58
15_P309_L1.1050.58
25_F287_R1.1040.58
7_E22_I1.1020.57
15_P52_L1.1020.57
27_C30_A1.1000.57
156_S160_E1.0980.57
20_E78_F1.0980.57
196_W200_V1.0960.57
118_S126_K1.0930.56
285_N288_R1.0910.56
112_S116_E1.0820.55
84_I131_S1.0820.55
175_L269_M1.0810.55
15_P18_F1.0800.55
123_A206_K1.0800.55
324_K327_E1.0780.55
51_G96_V1.0770.55
62_E94_V1.0760.55
314_E317_K1.0760.55
318_E321_N1.0750.54
98_L303_L1.0730.54
7_E10_L1.0710.54
52_L220_L1.0700.54
57_K161_D1.0700.54
41_E44_E1.0670.54
96_V126_K1.0660.54
20_E58_W1.0660.54
295_F299_L1.0580.53
134_D143_F1.0540.52
121_F125_M1.0540.52
9_C149_F1.0540.52
10_L17_V1.0540.52
77_I167_S1.0520.52
298_E302_T1.0470.52
234_K237_E1.0470.52
39_E42_N1.0470.52
9_C20_E1.0460.51
9_C15_P1.0460.51
246_E254_N1.0450.51
109_E214_G1.0390.51
52_L196_W1.0390.51
114_F139_V1.0380.51
247_P252_Q1.0370.50
37_S48_P1.0360.50
272_E319_K1.0320.50
303_L312_L1.0310.50
234_K267_N1.0300.50
165_F218_F1.0300.50
167_S223_I1.0260.49
56_F290_H1.0260.49
96_V182_R1.0220.49
318_E322_A1.0210.49
194_D296_I1.0190.49
169_V307_Q1.0170.48
72_S77_I1.0150.48
87_A125_M1.0140.48
246_E257_L1.0120.48
33_E210_K1.0120.48
56_F80_A1.0120.48
43_F213_E1.0090.48
200_V208_I1.0090.48
223_I230_I1.0070.47
107_L112_S1.0070.47
42_N224_V1.0070.47
38_L58_W1.0070.47
25_F110_T1.0060.47
163_F217_R1.0060.47
196_W309_L1.0030.47
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
4wlrA10.89971000.225Contact Map
4ig7A10.69911000.254Contact Map
3riiA20.65651000.384Contact Map
4i6nA20.64441000.398Contact Map
1xd3A20.66261000.427Contact Map
4jkjA20.65051000.43Contact Map
1cmxA20.61091000.439Contact Map
2wdtA20.61091000.44Contact Map
4cg4A60220.975Contact Map
4n78D10.33746.60.98Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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