| MalT180717 1-800 |
| ID: | 1501608556 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 800 (775) | ||
| Sequences: | 5153 (4031.8) | ||
| Seq/Len: | 6.649 | ||
| Nf(neff/√len): | 144.8 |
HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 6.649).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 585_R | 588_E | 6.615 | 1.00 |
| 543_F | 546_T | 6.330 | 1.00 |
| 624_D | 627_N | 5.585 | 1.00 |
| 665_D | 668_A | 5.330 | 1.00 |
| 49_I | 158_L | 4.931 | 1.00 |
| 344_H | 363_H | 4.864 | 1.00 |
| 424_R | 427_E | 4.851 | 1.00 |
| 464_N | 467_E | 4.573 | 1.00 |
| 743_R | 746_D | 4.436 | 1.00 |
| 503_E | 506_R | 4.309 | 1.00 |
| 703_E | 706_P | 4.150 | 1.00 |
| 548_W | 552_E | 3.250 | 1.00 |
| 191_A | 222_L | 3.240 | 1.00 |
| 535_S | 551_Q | 3.172 | 1.00 |
| 187_T | 190_E | 3.114 | 1.00 |
| 195_F | 226_A | 2.802 | 1.00 |
| 590_E | 622_R | 2.777 | 1.00 |
| 22_L | 48_L | 2.689 | 1.00 |
| 27_S | 55_G | 2.684 | 1.00 |
| 188_H | 209_S | 2.683 | 1.00 |
| 495_G | 511_M | 2.591 | 1.00 |
| 53_A | 126_V | 2.574 | 1.00 |
| 512_Q | 534_Q | 2.541 | 1.00 |
| 37_I | 49_I | 2.508 | 1.00 |
| 130_Y | 157_V | 2.492 | 1.00 |
| 26_L | 35_A | 2.466 | 1.00 |
| 352_M | 372_M | 2.454 | 1.00 |
| 548_W | 583_W | 2.430 | 1.00 |
| 712_E | 734_L | 2.383 | 1.00 |
| 719_R | 753_D | 2.376 | 1.00 |
| 39_S | 43_Y | 2.375 | 1.00 |
| 538_L | 543_F | 2.347 | 1.00 |
| 274_S | 320_Y | 2.337 | 1.00 |
| 508_L | 512_Q | 2.304 | 1.00 |
| 191_A | 212_C | 2.244 | 1.00 |
| 26_L | 52_W | 2.233 | 1.00 |
| 47_T | 51_Q | 2.213 | 1.00 |
| 41_A | 160_R | 2.206 | 1.00 |
| 508_L | 541_Q | 2.099 | 1.00 |
| 325_G | 329_R | 2.094 | 1.00 |
| 548_W | 576_R | 2.094 | 1.00 |
| 51_Q | 54_A | 2.071 | 1.00 |
| 670_A | 674_R | 2.070 | 1.00 |
| 616_I | 632_L | 2.011 | 1.00 |
| 735_N | 751_L | 2.004 | 1.00 |
| 590_E | 594_R | 2.000 | 1.00 |
| 538_L | 546_T | 1.998 | 1.00 |
| 552_E | 576_R | 1.996 | 1.00 |
| 49_I | 126_V | 1.977 | 1.00 |
| 629_R | 663_T | 1.963 | 1.00 |
| 264_D | 300_E | 1.959 | 1.00 |
| 580_L | 588_E | 1.952 | 1.00 |
| 480_P | 513_Q | 1.923 | 1.00 |
| 508_L | 534_Q | 1.904 | 1.00 |
| 580_L | 585_R | 1.870 | 1.00 |
| 415_Q | 419_M | 1.863 | 1.00 |
| 123_L | 153_L | 1.834 | 1.00 |
| 204_E | 207_E | 1.828 | 1.00 |
| 508_L | 538_L | 1.814 | 1.00 |
| 130_Y | 139_H | 1.809 | 1.00 |
| 348_A | 364_A | 1.798 | 1.00 |
| 27_S | 52_W | 1.796 | 1.00 |
| 416_A | 435_A | 1.796 | 1.00 |
| 267_T | 300_E | 1.791 | 1.00 |
| 49_I | 53_A | 1.790 | 1.00 |
| 16_T | 47_T | 1.784 | 1.00 |
| 619_S | 627_N | 1.782 | 1.00 |
| 734_L | 750_V | 1.758 | 1.00 |
| 268_R | 272_L | 1.754 | 1.00 |
| 531_L | 553_K | 1.750 | 1.00 |
| 629_R | 633_N | 1.741 | 1.00 |
| 590_E | 615_L | 1.735 | 1.00 |
| 275_A | 329_R | 1.718 | 1.00 |
| 498_L | 506_R | 1.718 | 1.00 |
| 708_E | 712_E | 1.710 | 1.00 |
| 277_L | 347_A | 1.697 | 1.00 |
| 345_R | 369_D | 1.689 | 1.00 |
| 429_N | 433_A | 1.659 | 1.00 |
| 577_A | 593_A | 1.656 | 1.00 |
| 193_Q | 197_C | 1.645 | 1.00 |
| 42_G | 45_K | 1.638 | 1.00 |
| 748_Q | 752_L | 1.631 | 1.00 |
| 345_R | 349_E | 1.628 | 1.00 |
| 559_N | 595_S | 1.625 | 1.00 |
| 738_Y | 746_D | 1.621 | 1.00 |
| 75_A | 78_L | 1.606 | 1.00 |
| 734_L | 738_Y | 1.605 | 1.00 |
| 288_V | 350_S | 1.599 | 1.00 |
| 491_T | 510_L | 1.591 | 1.00 |
| 188_H | 217_G | 1.580 | 1.00 |
| 534_Q | 550_T | 1.580 | 1.00 |
| 507_S | 537_I | 1.572 | 1.00 |
| 469_E | 473_K | 1.571 | 1.00 |
| 519_R | 553_K | 1.567 | 0.99 |
| 51_Q | 55_G | 1.564 | 0.99 |
| 580_L | 592_S | 1.563 | 0.99 |
| 271_L | 328_L | 1.561 | 0.99 |
| 128_D | 158_L | 1.547 | 0.99 |
| 211_I | 222_L | 1.547 | 0.99 |
| 18_V | 23_L | 1.545 | 0.99 |
| 618_C | 622_R | 1.540 | 0.99 |
| 270_F | 297_R | 1.538 | 0.99 |
| 419_M | 427_E | 1.531 | 0.99 |
| 518_A | 526_Y | 1.528 | 0.99 |
| 660_W | 665_D | 1.523 | 0.99 |
| 125_L | 155_L | 1.523 | 0.99 |
| 708_E | 737_L | 1.522 | 0.99 |
| 619_S | 631_Q | 1.519 | 0.99 |
| 531_L | 550_T | 1.506 | 0.99 |
| 619_S | 624_D | 1.504 | 0.99 |
| 588_E | 591_A | 1.498 | 0.99 |
| 731_L | 753_D | 1.497 | 0.99 |
| 53_A | 124_Y | 1.489 | 0.99 |
| 49_I | 156_V | 1.484 | 0.99 |
| 535_S | 554_A | 1.483 | 0.99 |
| 224_L | 259_V | 1.475 | 0.99 |
| 356_F | 359_E | 1.470 | 0.99 |
| 37_I | 158_L | 1.466 | 0.99 |
| 416_A | 432_L | 1.465 | 0.99 |
| 708_E | 741_A | 1.464 | 0.99 |
| 419_M | 431_L | 1.459 | 0.99 |
| 738_Y | 743_R | 1.458 | 0.99 |
| 307_F | 324_F | 1.457 | 0.99 |
| 759_N | 767_F | 1.450 | 0.99 |
| 576_R | 580_L | 1.445 | 0.99 |
| 660_W | 668_A | 1.440 | 0.99 |
| 538_L | 550_T | 1.438 | 0.99 |
| 188_H | 213_D | 1.435 | 0.99 |
| 264_D | 267_T | 1.431 | 0.99 |
| 192_K | 208_S | 1.430 | 0.99 |
| 35_A | 154_T | 1.428 | 0.99 |
| 160_R | 321_H | 1.418 | 0.99 |
| 731_L | 750_V | 1.412 | 0.99 |
| 369_D | 372_M | 1.408 | 0.99 |
| 171_R | 178_E | 1.400 | 0.99 |
| 552_E | 588_E | 1.397 | 0.99 |
| 191_A | 217_G | 1.395 | 0.99 |
| 506_R | 509_A | 1.391 | 0.99 |
| 58_D | 121_S | 1.390 | 0.98 |
| 495_G | 514_T | 1.388 | 0.98 |
| 186_F | 194_F | 1.387 | 0.98 |
| 528_L | 554_A | 1.387 | 0.98 |
| 263_V | 267_T | 1.386 | 0.98 |
| 25_K | 29_A | 1.379 | 0.98 |
| 508_L | 537_I | 1.379 | 0.98 |
| 436_E | 455_R | 1.372 | 0.98 |
| 134_T | 138_I | 1.368 | 0.98 |
| 552_E | 556_Q | 1.366 | 0.98 |
| 612_L | 631_Q | 1.349 | 0.98 |
| 594_R | 615_L | 1.337 | 0.98 |
| 39_S | 44_G | 1.325 | 0.98 |
| 587_D | 626_D | 1.321 | 0.98 |
| 715_N | 734_L | 1.319 | 0.98 |
| 544_L | 625_L | 1.317 | 0.98 |
| 514_T | 530_S | 1.313 | 0.98 |
| 342_E | 346_A | 1.304 | 0.97 |
| 39_S | 45_K | 1.300 | 0.97 |
| 37_I | 48_L | 1.300 | 0.97 |
| 698_Q | 703_E | 1.297 | 0.97 |
| 511_M | 593_A | 1.295 | 0.97 |
| 599_V | 638_L | 1.295 | 0.97 |
| 548_W | 585_R | 1.294 | 0.97 |
| 735_N | 754_A | 1.294 | 0.97 |
| 587_D | 622_R | 1.294 | 0.97 |
| 498_L | 619_S | 1.291 | 0.97 |
| 615_L | 631_Q | 1.291 | 0.97 |
| 765_S | 769_I | 1.286 | 0.97 |
| 628_A | 635_L | 1.284 | 0.97 |
| 477_E | 516_Q | 1.284 | 0.97 |
| 149_Q | 153_L | 1.284 | 0.97 |
| 626_D | 663_T | 1.283 | 0.97 |
| 216_S | 322_P | 1.277 | 0.97 |
| 588_E | 592_S | 1.277 | 0.97 |
| 697_A | 701_L | 1.276 | 0.97 |
| 520_Q | 641_N | 1.273 | 0.97 |
| 573_V | 592_S | 1.269 | 0.97 |
| 708_E | 743_R | 1.262 | 0.97 |
| 43_Y | 219_A | 1.257 | 0.97 |
| 543_F | 703_E | 1.257 | 0.97 |
| 516_Q | 556_Q | 1.256 | 0.97 |
| 209_S | 213_D | 1.254 | 0.97 |
| 715_N | 727_L | 1.250 | 0.97 |
| 551_Q | 575_I | 1.249 | 0.96 |
| 194_F | 222_L | 1.249 | 0.96 |
| 389_E | 392_L | 1.249 | 0.96 |
| 708_E | 734_L | 1.247 | 0.96 |
| 22_L | 190_E | 1.247 | 0.96 |
| 747_A | 751_L | 1.243 | 0.96 |
| 537_I | 541_Q | 1.242 | 0.96 |
| 513_Q | 553_K | 1.238 | 0.96 |
| 548_W | 580_L | 1.234 | 0.96 |
| 549_E | 591_A | 1.234 | 0.96 |
| 36_L | 159_S | 1.232 | 0.96 |
| 502_G | 542_G | 1.230 | 0.96 |
| 39_S | 42_G | 1.229 | 0.96 |
| 670_A | 701_L | 1.228 | 0.96 |
| 738_Y | 750_V | 1.228 | 0.96 |
| 715_N | 730_N | 1.224 | 0.96 |
| 270_F | 294_G | 1.222 | 0.96 |
| 515_E | 534_Q | 1.221 | 0.96 |
| 128_D | 131_H | 1.220 | 0.96 |
| 590_E | 619_S | 1.220 | 0.96 |
| 19_R | 22_L | 1.220 | 0.96 |
| 42_G | 46_T | 1.219 | 0.96 |
| 549_E | 671_N | 1.217 | 0.96 |
| 469_E | 494_L | 1.217 | 0.96 |
| 43_Y | 218_W | 1.216 | 0.96 |
| 553_K | 634_R | 1.216 | 0.96 |
| 433_A | 437_H | 1.210 | 0.96 |
| 265_L | 268_R | 1.208 | 0.96 |
| 668_A | 671_N | 1.205 | 0.95 |
| 272_L | 331_R | 1.201 | 0.95 |
| 473_K | 477_E | 1.196 | 0.95 |
| 531_L | 557_L | 1.191 | 0.95 |
| 590_E | 618_C | 1.189 | 0.95 |
| 210_R | 213_D | 1.187 | 0.95 |
| 216_S | 319_C | 1.184 | 0.95 |
| 698_Q | 706_P | 1.182 | 0.95 |
| 393_L | 396_S | 1.179 | 0.95 |
| 573_V | 595_S | 1.178 | 0.95 |
| 719_R | 727_L | 1.176 | 0.95 |
| 210_R | 214_D | 1.174 | 0.95 |
| 146_I | 155_L | 1.173 | 0.95 |
| 29_A | 35_A | 1.165 | 0.94 |
| 186_F | 190_E | 1.164 | 0.94 |
| 713_E | 717_N | 1.163 | 0.94 |
| 284_L | 350_S | 1.162 | 0.94 |
| 541_Q | 701_L | 1.162 | 0.94 |
| 577_A | 589_A | 1.160 | 0.94 |
| 546_T | 627_N | 1.158 | 0.94 |
| 465_P | 544_L | 1.158 | 0.94 |
| 519_R | 527_A | 1.158 | 0.94 |
| 270_F | 285_I | 1.155 | 0.94 |
| 594_R | 633_N | 1.154 | 0.94 |
| 550_T | 592_S | 1.151 | 0.94 |
| 286_T | 294_G | 1.150 | 0.94 |
| 16_T | 19_R | 1.145 | 0.94 |
| 633_N | 674_R | 1.144 | 0.94 |
| 483_W | 644_Y | 1.142 | 0.93 |
| 16_T | 51_Q | 1.142 | 0.93 |
| 44_G | 48_L | 1.142 | 0.93 |
| 509_A | 630_S | 1.142 | 0.93 |
| 555_F | 576_R | 1.142 | 0.93 |
| 470_R | 509_A | 1.140 | 0.93 |
| 195_F | 208_S | 1.139 | 0.93 |
| 498_L | 510_L | 1.134 | 0.93 |
| 465_P | 504_L | 1.132 | 0.93 |
| 467_E | 546_T | 1.130 | 0.93 |
| 708_E | 738_Y | 1.127 | 0.93 |
| 548_W | 579_L | 1.127 | 0.93 |
| 491_T | 513_Q | 1.125 | 0.93 |
| 535_S | 547_A | 1.124 | 0.93 |
| 667_A | 705_E | 1.118 | 0.92 |
| 192_K | 205_A | 1.118 | 0.92 |
| 280_M | 285_I | 1.116 | 0.92 |
| 476_L | 555_F | 1.115 | 0.92 |
| 699_I | 737_L | 1.115 | 0.92 |
| 288_V | 347_A | 1.115 | 0.92 |
| 556_Q | 637_N | 1.114 | 0.92 |
| 47_T | 223_Q | 1.113 | 0.92 |
| 46_T | 50_S | 1.109 | 0.92 |
| 589_A | 618_C | 1.109 | 0.92 |
| 504_L | 537_I | 1.106 | 0.92 |
| 601_S | 608_Q | 1.104 | 0.92 |
| 511_M | 537_I | 1.102 | 0.92 |
| 38_T | 159_S | 1.102 | 0.92 |
| 636_E | 656_R | 1.101 | 0.92 |
| 20_E | 24_A | 1.101 | 0.92 |
| 612_L | 634_R | 1.095 | 0.91 |
| 267_T | 270_F | 1.094 | 0.91 |
| 728_N | 757_L | 1.089 | 0.91 |
| 456_A | 472_A | 1.088 | 0.91 |
| 21_R | 25_K | 1.088 | 0.91 |
| 474_L | 595_S | 1.085 | 0.91 |
| 707_A | 737_L | 1.085 | 0.91 |
| 616_I | 628_A | 1.084 | 0.91 |
| 364_A | 372_M | 1.083 | 0.91 |
| 215_V | 222_L | 1.083 | 0.91 |
| 532_I | 575_I | 1.083 | 0.91 |
| 506_R | 510_L | 1.081 | 0.91 |
| 272_L | 339_E | 1.080 | 0.91 |
| 455_R | 459_A | 1.079 | 0.91 |
| 462_D | 622_R | 1.078 | 0.91 |
| 329_R | 340_L | 1.078 | 0.91 |
| 534_Q | 538_L | 1.075 | 0.90 |
| 474_L | 477_E | 1.074 | 0.90 |
| 590_E | 624_D | 1.073 | 0.90 |
| 494_L | 498_L | 1.071 | 0.90 |
| 671_N | 709_I | 1.070 | 0.90 |
| 513_Q | 634_R | 1.070 | 0.90 |
| 544_L | 579_L | 1.065 | 0.90 |
| 575_I | 614_M | 1.064 | 0.90 |
| 756_K | 760_R | 1.063 | 0.90 |
| 544_L | 586_L | 1.062 | 0.90 |
| 586_L | 625_L | 1.062 | 0.90 |
| 516_Q | 598_E | 1.060 | 0.90 |
| 248_A | 251_L | 1.060 | 0.90 |
| 724_M | 727_L | 1.060 | 0.90 |
| 511_M | 551_Q | 1.058 | 0.89 |
| 699_I | 733_L | 1.058 | 0.89 |
| 758_A | 761_T | 1.057 | 0.89 |
| 593_A | 632_L | 1.057 | 0.89 |
| 627_N | 631_Q | 1.056 | 0.89 |
| 470_R | 549_E | 1.055 | 0.89 |
| 198_R | 223_Q | 1.053 | 0.89 |
| 505_T | 541_Q | 1.052 | 0.89 |
| 473_K | 552_E | 1.049 | 0.89 |
| 190_E | 193_Q | 1.048 | 0.89 |
| 579_L | 583_W | 1.048 | 0.89 |
| 129_D | 132_L | 1.047 | 0.89 |
| 518_A | 530_S | 1.045 | 0.89 |
| 553_K | 556_Q | 1.042 | 0.89 |
| 544_L | 666_K | 1.039 | 0.88 |
| 130_Y | 133_I | 1.038 | 0.88 |
| 224_L | 227_L | 1.038 | 0.88 |
| 470_R | 630_S | 1.036 | 0.88 |
| 547_A | 579_L | 1.036 | 0.88 |
| 340_L | 344_H | 1.035 | 0.88 |
| 576_R | 592_S | 1.031 | 0.88 |
| 549_E | 553_K | 1.031 | 0.88 |
| 706_P | 709_I | 1.031 | 0.88 |
| 144_F | 148_H | 1.031 | 0.88 |
| 45_K | 48_L | 1.029 | 0.88 |
| 23_L | 51_Q | 1.027 | 0.88 |
| 392_L | 396_S | 1.026 | 0.87 |
| 547_A | 554_A | 1.024 | 0.87 |
| 714_L | 730_N | 1.022 | 0.87 |
| 634_R | 675_H | 1.022 | 0.87 |
| 591_A | 671_N | 1.021 | 0.87 |
| 52_W | 124_Y | 1.018 | 0.87 |
| 577_A | 615_L | 1.018 | 0.87 |
| 478_E | 517_M | 1.017 | 0.87 |
| 25_K | 183_Q | 1.015 | 0.87 |
| 467_E | 470_R | 1.015 | 0.87 |
| 50_S | 61_W | 1.014 | 0.87 |
| 771_G | 775_A | 1.013 | 0.87 |
| 367_A | 373_L | 1.013 | 0.87 |
| 555_F | 569_H | 1.011 | 0.86 |
| 434_R | 513_Q | 1.010 | 0.86 |
| 6_K | 77_Y | 1.009 | 0.86 |
| 40_P | 43_Y | 1.008 | 0.86 |
| 160_R | 323_L | 1.008 | 0.86 |
| 554_A | 635_L | 1.008 | 0.86 |
| 698_Q | 738_Y | 1.007 | 0.86 |
| 735_N | 747_A | 1.006 | 0.86 |
| 287_R | 353_A | 1.006 | 0.86 |
| 360_A | 364_A | 1.004 | 0.86 |
| 511_M | 534_Q | 1.001 | 0.86 |
| 626_D | 705_E | 1.001 | 0.86 |
| 545_Q | 667_A | 1.001 | 0.86 |
| 505_T | 545_Q | 1.000 | 0.86 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 1z6tA | 4 | 0.5337 | 100 | 0.673 | Contact Map |
| 1hz4A | 1 | 0.4537 | 100 | 0.705 | Contact Map |
| 2a5yB | 2 | 0.4325 | 100 | 0.724 | Contact Map |
| 3sf4A | 3 | 0.4225 | 100 | 0.756 | Contact Map |
| 4ui9O | 1 | 0 | 100 | 0.762 | Contact Map |
| 2qenA | 1 | 0.39 | 100 | 0.765 | Contact Map |
| 4a1sA | 2 | 0.3875 | 100 | 0.768 | Contact Map |
| 3ro2A | 1 | 0.3862 | 99.9 | 0.771 | Contact Map |
| 1w3bA | 2 | 0.4838 | 99.9 | 0.773 | Contact Map |
| 2xpiA | 2 | 0.565 | 99.9 | 0.774 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)