| hHsp70 |
| ID: | 1501601455 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 641 (627) | ||
| Sequences: | 8390 (4180.3) | ||
| Seq/Len: | 13.381 | ||
| Nf(neff/√len): | 166.9 |
Jackhmmer Results: (2016_04)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 13.381).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 141_N | 168_N | 4.861 | 1.00 |
| 225_D | 312_S | 4.701 | 1.00 |
| 236_R | 308_D | 4.266 | 1.00 |
| 351_Q | 358_D | 3.740 | 1.00 |
| 69_D | 231_E | 3.429 | 1.00 |
| 348_K | 352_D | 3.405 | 1.00 |
| 237_L | 297_I | 3.379 | 1.00 |
| 279_Q | 296_S | 3.365 | 1.00 |
| 198_F | 313_T | 3.356 | 1.00 |
| 31_N | 38_T | 3.196 | 1.00 |
| 209_I | 320_A | 3.179 | 1.00 |
| 400_S | 412_A | 3.085 | 1.00 |
| 242_V | 254_S | 2.948 | 1.00 |
| 441_Q | 455_L | 2.895 | 1.00 |
| 364_N | 367_E | 2.795 | 1.00 |
| 439_L | 460_E | 2.741 | 1.00 |
| 241_F | 295_T | 2.733 | 1.00 |
| 568_K | 572_D | 2.718 | 1.00 |
| 550_K | 578_I | 2.718 | 1.00 |
| 244_E | 301_R | 2.717 | 1.00 |
| 141_N | 170_L | 2.694 | 1.00 |
| 200_L | 233_F | 2.669 | 1.00 |
| 512_K | 516_E | 2.612 | 1.00 |
| 411_T | 428_F | 2.541 | 1.00 |
| 221_A | 320_A | 2.517 | 1.00 |
| 412_A | 415_K | 2.504 | 1.00 |
| 575_Q | 579_S | 2.470 | 1.00 |
| 35_N | 53_D | 2.451 | 1.00 |
| 79_G | 96_N | 2.415 | 1.00 |
| 196_L | 209_I | 2.345 | 1.00 |
| 347_Q | 361_K | 2.334 | 1.00 |
| 64_Q | 93_Q | 2.334 | 1.00 |
| 6_A | 170_L | 2.333 | 1.00 |
| 307_S | 311_R | 2.330 | 1.00 |
| 196_L | 207_V | 2.326 | 1.00 |
| 525_Y | 528_E | 2.307 | 1.00 |
| 301_R | 304_E | 2.269 | 1.00 |
| 310_F | 345_K | 2.264 | 1.00 |
| 402_G | 410_M | 2.198 | 1.00 |
| 608_S | 612_Q | 2.186 | 1.00 |
| 517_R | 521_E | 2.183 | 1.00 |
| 579_S | 582_D | 2.180 | 1.00 |
| 302_F | 343_I | 2.176 | 1.00 |
| 340_S | 343_I | 2.168 | 1.00 |
| 588_E | 591_E | 2.146 | 1.00 |
| 349_L | 352_D | 2.141 | 1.00 |
| 559_K | 567_K | 2.135 | 1.00 |
| 7_I | 18_V | 2.116 | 1.00 |
| 523_E | 526_K | 2.112 | 1.00 |
| 128_K | 132_E | 2.111 | 1.00 |
| 491_T | 498_A | 2.048 | 1.00 |
| 530_E | 534_E | 2.031 | 1.00 |
| 235_N | 239_N | 2.023 | 1.00 |
| 163_V | 168_N | 2.020 | 1.00 |
| 22_Q | 27_E | 2.009 | 1.00 |
| 315_E | 319_K | 2.003 | 1.00 |
| 589_K | 593_E | 2.002 | 1.00 |
| 315_E | 318_E | 2.000 | 1.00 |
| 306_C | 343_I | 1.999 | 1.00 |
| 233_F | 309_L | 1.995 | 1.00 |
| 314_L | 353_F | 1.993 | 1.00 |
| 443_Y | 455_L | 1.977 | 1.00 |
| 281_S | 294_Y | 1.962 | 1.00 |
| 593_E | 597_K | 1.960 | 1.00 |
| 49_R | 122_M | 1.958 | 1.00 |
| 563_S | 566_D | 1.954 | 1.00 |
| 244_E | 247_R | 1.915 | 1.00 |
| 241_F | 267_C | 1.911 | 1.00 |
| 318_E | 322_R | 1.858 | 1.00 |
| 306_C | 310_F | 1.842 | 1.00 |
| 527_A | 531_V | 1.837 | 1.00 |
| 597_K | 601_Q | 1.831 | 1.00 |
| 145_T | 373_A | 1.824 | 1.00 |
| 129_E | 133_A | 1.822 | 1.00 |
| 566_D | 609_G | 1.817 | 1.00 |
| 238_V | 264_R | 1.817 | 1.00 |
| 571_L | 575_Q | 1.810 | 1.00 |
| 19_G | 374_A | 1.810 | 1.00 |
| 572_D | 576_E | 1.781 | 1.00 |
| 84_Q | 87_M | 1.776 | 1.00 |
| 410_M | 443_Y | 1.764 | 1.00 |
| 520_Q | 523_E | 1.760 | 1.00 |
| 114_F | 119_I | 1.740 | 1.00 |
| 6_A | 377_A | 1.736 | 1.00 |
| 394_L | 419_T | 1.730 | 1.00 |
| 564_E | 568_K | 1.724 | 1.00 |
| 516_E | 520_Q | 1.724 | 1.00 |
| 64_Q | 257_K | 1.720 | 1.00 |
| 542_L | 577_V | 1.716 | 1.00 |
| 520_Q | 524_K | 1.710 | 1.00 |
| 327_D | 330_Q | 1.709 | 1.00 |
| 605_P | 608_S | 1.699 | 1.00 |
| 249_H | 291_I | 1.699 | 1.00 |
| 117_E | 160_D | 1.697 | 1.00 |
| 314_L | 318_E | 1.686 | 1.00 |
| 318_E | 353_F | 1.684 | 1.00 |
| 266_A | 270_A | 1.681 | 1.00 |
| 302_F | 340_S | 1.662 | 1.00 |
| 193_R | 333_D | 1.662 | 1.00 |
| 595_K | 598_E | 1.656 | 1.00 |
| 439_L | 458_R | 1.651 | 1.00 |
| 163_V | 512_K | 1.648 | 1.00 |
| 576_E | 595_K | 1.643 | 1.00 |
| 573_K | 576_E | 1.643 | 1.00 |
| 549_M | 603_C | 1.636 | 1.00 |
| 65_N | 110_E | 1.615 | 1.00 |
| 44_F | 122_M | 1.611 | 1.00 |
| 268_E | 272_R | 1.608 | 1.00 |
| 441_Q | 458_R | 1.606 | 1.00 |
| 17_C | 37_T | 1.590 | 1.00 |
| 303_E | 348_K | 1.590 | 1.00 |
| 156_Q | 160_D | 1.575 | 1.00 |
| 270_A | 282_L | 1.567 | 1.00 |
| 567_K | 571_L | 1.564 | 1.00 |
| 319_K | 323_D | 1.559 | 1.00 |
| 17_C | 28_I | 1.557 | 1.00 |
| 342_R | 366_D | 1.555 | 1.00 |
| 243_E | 254_S | 1.552 | 1.00 |
| 209_I | 326_L | 1.550 | 1.00 |
| 241_F | 263_L | 1.547 | 1.00 |
| 397_A | 480_I | 1.540 | 1.00 |
| 126_K | 130_I | 1.540 | 1.00 |
| 573_K | 598_E | 1.539 | 1.00 |
| 304_E | 307_S | 1.526 | 1.00 |
| 321_L | 328_K | 1.521 | 1.00 |
| 412_A | 448_A | 1.517 | 1.00 |
| 36_R | 367_E | 1.515 | 1.00 |
| 127_M | 130_I | 1.510 | 1.00 |
| 546_A | 578_I | 1.498 | 1.00 |
| 298_T | 301_R | 1.496 | 1.00 |
| 600_E | 604_N | 1.494 | 1.00 |
| 246_K | 252_D | 1.493 | 1.00 |
| 104_Q | 111_T | 1.492 | 1.00 |
| 270_A | 280_A | 1.489 | 1.00 |
| 333_D | 360_N | 1.487 | 1.00 |
| 590_D | 594_H | 1.480 | 1.00 |
| 455_L | 458_R | 1.478 | 1.00 |
| 449_M | 536_V | 1.477 | 1.00 |
| 604_N | 607_I | 1.476 | 1.00 |
| 585_T | 588_E | 1.476 | 1.00 |
| 564_E | 571_L | 1.472 | 1.00 |
| 240_H | 301_R | 1.471 | 1.00 |
| 301_R | 305_L | 1.471 | 1.00 |
| 43_A | 58_Q | 1.459 | 1.00 |
| 253_I | 287_L | 1.457 | 1.00 |
| 418_S | 422_T | 1.452 | 1.00 |
| 233_F | 302_F | 1.451 | 1.00 |
| 405_T | 411_T | 1.448 | 1.00 |
| 449_M | 452_D | 1.447 | 1.00 |
| 223_A | 316_P | 1.444 | 1.00 |
| 594_H | 598_E | 1.444 | 1.00 |
| 241_F | 282_L | 1.438 | 1.00 |
| 345_K | 349_L | 1.427 | 1.00 |
| 122_M | 125_T | 1.425 | 1.00 |
| 96_N | 99_D | 1.414 | 1.00 |
| 42_V | 119_I | 1.408 | 1.00 |
| 348_K | 351_Q | 1.402 | 0.99 |
| 603_C | 607_I | 1.402 | 0.99 |
| 473_Q | 493_K | 1.398 | 0.99 |
| 511_S | 514_E | 1.398 | 0.99 |
| 546_A | 577_V | 1.397 | 0.99 |
| 527_A | 530_E | 1.396 | 0.99 |
| 194_N | 326_L | 1.393 | 0.99 |
| 345_K | 348_K | 1.392 | 0.99 |
| 239_N | 243_E | 1.392 | 0.99 |
| 104_Q | 113_A | 1.388 | 0.99 |
| 29_I | 134_Y | 1.384 | 0.99 |
| 19_G | 370_A | 1.384 | 0.99 |
| 209_I | 331_I | 1.380 | 0.99 |
| 68_F | 91_P | 1.370 | 0.99 |
| 514_E | 517_R | 1.355 | 0.99 |
| 546_A | 574_C | 1.352 | 0.99 |
| 194_N | 220_K | 1.346 | 0.99 |
| 240_H | 244_E | 1.339 | 0.99 |
| 11_L | 158_T | 1.338 | 0.99 |
| 579_S | 583_A | 1.335 | 0.99 |
| 425_T | 475_E | 1.334 | 0.99 |
| 427_I | 475_E | 1.326 | 0.99 |
| 319_K | 322_R | 1.318 | 0.99 |
| 16_S | 127_M | 1.318 | 0.99 |
| 314_L | 349_L | 1.316 | 0.99 |
| 223_A | 319_K | 1.312 | 0.99 |
| 244_E | 295_T | 1.310 | 0.99 |
| 236_R | 306_C | 1.308 | 0.99 |
| 21_F | 378_A | 1.307 | 0.99 |
| 70_A | 119_I | 1.297 | 0.99 |
| 200_L | 205_F | 1.297 | 0.99 |
| 578_I | 582_D | 1.296 | 0.99 |
| 144_I | 162_G | 1.296 | 0.99 |
| 318_E | 328_K | 1.295 | 0.99 |
| 538_A | 593_E | 1.290 | 0.99 |
| 404_E | 441_Q | 1.289 | 0.99 |
| 240_H | 295_T | 1.289 | 0.99 |
| 160_D | 163_V | 1.287 | 0.99 |
| 120_S | 158_T | 1.287 | 0.99 |
| 310_F | 349_L | 1.285 | 0.99 |
| 591_E | 595_K | 1.282 | 0.99 |
| 128_K | 167_L | 1.279 | 0.99 |
| 425_T | 477_T | 1.276 | 0.99 |
| 44_F | 114_F | 1.272 | 0.99 |
| 350_L | 359_L | 1.268 | 0.99 |
| 159_K | 485_I | 1.265 | 0.99 |
| 30_A | 134_Y | 1.262 | 0.99 |
| 18_V | 127_M | 1.261 | 0.99 |
| 607_I | 611_Y | 1.261 | 0.99 |
| 588_E | 592_F | 1.258 | 0.99 |
| 609_G | 612_Q | 1.252 | 0.99 |
| 62_N | 65_N | 1.248 | 0.99 |
| 70_A | 120_S | 1.246 | 0.98 |
| 118_E | 122_M | 1.243 | 0.98 |
| 162_G | 172_I | 1.243 | 0.98 |
| 307_S | 345_K | 1.241 | 0.98 |
| 220_K | 326_L | 1.233 | 0.98 |
| 32_D | 130_I | 1.231 | 0.98 |
| 266_A | 269_R | 1.230 | 0.98 |
| 239_N | 254_S | 1.229 | 0.98 |
| 299_R | 303_E | 1.222 | 0.98 |
| 70_A | 123_V | 1.222 | 0.98 |
| 237_L | 267_C | 1.216 | 0.98 |
| 521_E | 524_K | 1.216 | 0.98 |
| 528_E | 531_V | 1.215 | 0.98 |
| 240_H | 297_I | 1.209 | 0.98 |
| 566_D | 569_K | 1.204 | 0.98 |
| 565_A | 569_K | 1.201 | 0.98 |
| 594_H | 597_K | 1.200 | 0.98 |
| 534_E | 589_K | 1.196 | 0.98 |
| 28_I | 371_Y | 1.193 | 0.98 |
| 518_M | 521_E | 1.193 | 0.98 |
| 93_Q | 104_Q | 1.192 | 0.98 |
| 86_D | 227_H | 1.189 | 0.98 |
| 233_F | 306_C | 1.186 | 0.98 |
| 95_I | 113_A | 1.185 | 0.98 |
| 174_N | 177_T | 1.185 | 0.98 |
| 569_K | 573_K | 1.183 | 0.98 |
| 270_A | 297_I | 1.179 | 0.98 |
| 194_N | 211_T | 1.178 | 0.98 |
| 170_L | 380_L | 1.172 | 0.98 |
| 196_L | 317_V | 1.165 | 0.97 |
| 411_T | 426_Q | 1.164 | 0.97 |
| 240_H | 305_L | 1.163 | 0.97 |
| 282_L | 297_I | 1.162 | 0.97 |
| 596_R | 599_L | 1.155 | 0.97 |
| 58_Q | 62_N | 1.155 | 0.97 |
| 423_K | 477_T | 1.152 | 0.97 |
| 283_E | 294_Y | 1.152 | 0.97 |
| 538_A | 541_A | 1.151 | 0.97 |
| 538_A | 596_R | 1.145 | 0.97 |
| 323_D | 416_R | 1.144 | 0.97 |
| 30_A | 36_R | 1.142 | 0.97 |
| 541_A | 596_R | 1.140 | 0.97 |
| 320_A | 324_A | 1.137 | 0.97 |
| 244_E | 293_F | 1.136 | 0.97 |
| 573_K | 577_V | 1.135 | 0.97 |
| 596_R | 600_E | 1.130 | 0.97 |
| 592_F | 596_R | 1.129 | 0.97 |
| 437_G | 460_E | 1.125 | 0.97 |
| 465_P | 494_S | 1.125 | 0.97 |
| 87_M | 94_V | 1.122 | 0.97 |
| 592_F | 599_L | 1.122 | 0.97 |
| 443_Y | 450_T | 1.121 | 0.97 |
| 67_V | 119_I | 1.119 | 0.96 |
| 84_Q | 88_K | 1.118 | 0.96 |
| 211_T | 220_K | 1.117 | 0.96 |
| 238_V | 267_C | 1.117 | 0.96 |
| 125_T | 129_E | 1.114 | 0.96 |
| 311_R | 349_L | 1.114 | 0.96 |
| 569_K | 602_V | 1.111 | 0.96 |
| 556_E | 559_K | 1.106 | 0.96 |
| 526_K | 530_E | 1.105 | 0.96 |
| 41_Y | 68_F | 1.101 | 0.96 |
| 97_D | 102_K | 1.096 | 0.96 |
| 233_F | 310_F | 1.089 | 0.96 |
| 535_R | 539_K | 1.088 | 0.96 |
| 604_N | 608_S | 1.084 | 0.96 |
| 516_E | 519_V | 1.081 | 0.95 |
| 16_S | 40_S | 1.078 | 0.95 |
| 162_G | 169_V | 1.078 | 0.95 |
| 79_G | 99_D | 1.076 | 0.95 |
| 549_M | 574_C | 1.076 | 0.95 |
| 233_F | 343_I | 1.074 | 0.95 |
| 589_K | 592_F | 1.074 | 0.95 |
| 437_G | 462_S | 1.071 | 0.95 |
| 242_V | 260_V | 1.070 | 0.95 |
| 605_P | 609_G | 1.069 | 0.95 |
| 7_I | 139_V | 1.068 | 0.95 |
| 242_V | 263_L | 1.063 | 0.95 |
| 223_A | 323_D | 1.062 | 0.95 |
| 533_R | 537_S | 1.057 | 0.95 |
| 321_L | 326_L | 1.053 | 0.95 |
| 43_A | 50_L | 1.051 | 0.94 |
| 568_K | 571_L | 1.048 | 0.94 |
| 13_T | 203_G | 1.046 | 0.94 |
| 11_L | 120_S | 1.045 | 0.94 |
| 591_E | 594_H | 1.035 | 0.94 |
| 590_D | 593_E | 1.034 | 0.94 |
| 87_M | 93_Q | 1.031 | 0.94 |
| 45_T | 48_E | 1.030 | 0.94 |
| 236_R | 309_L | 1.029 | 0.94 |
| 246_K | 254_S | 1.025 | 0.93 |
| 132_E | 138_P | 1.023 | 0.93 |
| 326_L | 330_Q | 1.021 | 0.93 |
| 70_A | 74_I | 1.018 | 0.93 |
| 235_N | 238_V | 1.018 | 0.93 |
| 174_N | 395_D | 1.017 | 0.93 |
| 562_I | 567_K | 1.017 | 0.93 |
| 266_A | 280_A | 1.015 | 0.93 |
| 607_I | 610_L | 1.011 | 0.93 |
| 360_N | 368_A | 1.005 | 0.93 |
| 306_C | 309_L | 1.005 | 0.93 |
| 210_L | 219_V | 1.005 | 0.93 |
| 20_V | 135_L | 1.005 | 0.93 |
| 545_Y | 549_M | 1.005 | 0.93 |
| 573_K | 602_V | 1.003 | 0.93 |
| 554_E | 559_K | 1.000 | 0.92 |
| 494_S | 611_Y | 1.000 | 0.92 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 3d2fA | 2 | 0.9563 | 100 | 0.166 | Contact Map |
| 4jneA | 2 | 0.922 | 100 | 0.228 | Contact Map |
| 4fl9A | 1 | 0.8409 | 100 | 0.231 | Contact Map |
| 2v7yA | 1 | 0.78 | 100 | 0.315 | Contact Map |
| 4rtfD | 1 | 0.5382 | 100 | 0.328 | Contact Map |
| 3i33A | 1 | 0.5897 | 100 | 0.471 | Contact Map |
| 4j8fA | 1 | 0.8518 | 100 | 0.487 | Contact Map |
| 4kboA | 1 | 0.5772 | 100 | 0.507 | Contact Map |
| 4gniA | 2 | 0.5819 | 100 | 0.539 | Contact Map |
| 3qfuA | 1 | 0.5897 | 100 | 0.54 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)