| BmdB_Cy |
| ID: | 1501514306 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 746 (739) | ||
| Sequences: | 12841 (10767.1) | ||
| Seq/Len: | 17.376 | ||
| Nf(neff/√len): | 396.1 |
HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 17.376).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 712_T | 729_I | 5.007 | 1.00 |
| 635_R | 661_V | 3.842 | 1.00 |
| 633_G | 711_D | 3.742 | 1.00 |
| 395_R | 426_D | 3.589 | 1.00 |
| 678_N | 717_Y | 3.367 | 1.00 |
| 663_I | 672_Q | 3.285 | 1.00 |
| 191_V | 201_L | 3.170 | 1.00 |
| 738_T | 742_I | 3.098 | 1.00 |
| 164_E | 257_T | 2.932 | 1.00 |
| 433_Q | 441_Q | 2.906 | 1.00 |
| 615_D | 655_K | 2.899 | 1.00 |
| 619_N | 655_K | 2.727 | 1.00 |
| 624_F | 628_R | 2.662 | 1.00 |
| 731_T | 734_A | 2.646 | 1.00 |
| 590_I | 615_D | 2.565 | 1.00 |
| 615_D | 702_D | 2.553 | 1.00 |
| 612_S | 616_Q | 2.543 | 1.00 |
| 637_G | 681_C | 2.538 | 1.00 |
| 390_W | 543_Q | 2.527 | 1.00 |
| 368_A | 378_L | 2.513 | 1.00 |
| 592_Q | 596_T | 2.509 | 1.00 |
| 344_I | 533_D | 2.499 | 1.00 |
| 621_V | 648_I | 2.498 | 1.00 |
| 738_T | 741_D | 2.491 | 1.00 |
| 737_N | 742_I | 2.457 | 1.00 |
| 386_V | 547_L | 2.427 | 1.00 |
| 630_I | 658_A | 2.407 | 1.00 |
| 435_F | 551_L | 2.398 | 1.00 |
| 325_K | 445_T | 2.385 | 1.00 |
| 634_D | 682_K | 2.356 | 1.00 |
| 592_Q | 595_R | 2.310 | 1.00 |
| 179_Q | 183_Q | 2.298 | 1.00 |
| 621_V | 652_A | 2.297 | 1.00 |
| 583_A | 591_D | 2.290 | 1.00 |
| 632_R | 711_D | 2.276 | 1.00 |
| 525_W | 537_M | 2.258 | 1.00 |
| 534_A | 538_E | 2.241 | 1.00 |
| 635_R | 715_V | 2.225 | 1.00 |
| 318_A | 452_H | 2.207 | 1.00 |
| 619_N | 623_R | 2.204 | 1.00 |
| 181_V | 284_Y | 2.203 | 1.00 |
| 371_K | 436_F | 2.171 | 1.00 |
| 674_Y | 724_R | 2.161 | 1.00 |
| 596_T | 599_Q | 2.151 | 1.00 |
| 184_R | 280_D | 2.115 | 1.00 |
| 166_E | 257_T | 2.090 | 1.00 |
| 384_G | 427_M | 2.071 | 1.00 |
| 634_D | 658_A | 2.068 | 1.00 |
| 630_I | 634_D | 2.060 | 1.00 |
| 600_V | 608_W | 2.053 | 1.00 |
| 525_W | 541_F | 2.034 | 1.00 |
| 264_Y | 269_M | 2.033 | 1.00 |
| 397_A | 424_L | 2.000 | 1.00 |
| 175_S | 179_Q | 1.993 | 1.00 |
| 710_E | 731_T | 1.991 | 1.00 |
| 618_S | 655_K | 1.959 | 1.00 |
| 145_M | 149_N | 1.952 | 1.00 |
| 407_P | 411_E | 1.947 | 1.00 |
| 585_L | 735_A | 1.945 | 1.00 |
| 364_L | 375_P | 1.919 | 1.00 |
| 631_G | 634_D | 1.900 | 1.00 |
| 584_T | 587_Q | 1.899 | 1.00 |
| 676_L | 681_C | 1.878 | 1.00 |
| 95_G | 98_D | 1.872 | 1.00 |
| 383_Y | 478_I | 1.871 | 1.00 |
| 638_V | 649_N | 1.871 | 1.00 |
| 277_V | 416_V | 1.866 | 1.00 |
| 570_I | 573_Y | 1.861 | 1.00 |
| 395_R | 428_D | 1.855 | 1.00 |
| 54_T | 83_I | 1.851 | 1.00 |
| 144_Y | 147_G | 1.849 | 1.00 |
| 667_H | 675_I | 1.845 | 1.00 |
| 615_D | 619_N | 1.843 | 1.00 |
| 734_A | 737_N | 1.840 | 1.00 |
| 617_R | 645_E | 1.832 | 1.00 |
| 609_L | 648_I | 1.829 | 1.00 |
| 245_L | 268_L | 1.819 | 1.00 |
| 181_V | 281_L | 1.819 | 1.00 |
| 708_E | 711_D | 1.816 | 1.00 |
| 174_L | 281_L | 1.801 | 1.00 |
| 635_R | 659_A | 1.801 | 1.00 |
| 191_V | 243_W | 1.801 | 1.00 |
| 604_F | 644_V | 1.781 | 1.00 |
| 460_F | 464_Y | 1.780 | 1.00 |
| 319_K | 455_Y | 1.779 | 1.00 |
| 598_D | 612_S | 1.776 | 1.00 |
| 674_Y | 717_Y | 1.768 | 1.00 |
| 70_F | 75_G | 1.767 | 1.00 |
| 710_E | 733_Q | 1.764 | 1.00 |
| 514_V | 523_V | 1.736 | 1.00 |
| 279_Q | 414_Q | 1.734 | 1.00 |
| 144_Y | 148_R | 1.718 | 1.00 |
| 623_R | 703_L | 1.707 | 1.00 |
| 739_I | 742_I | 1.705 | 1.00 |
| 652_A | 656_A | 1.700 | 1.00 |
| 436_F | 556_D | 1.692 | 1.00 |
| 434_P | 437_T | 1.688 | 1.00 |
| 180_K | 183_Q | 1.685 | 1.00 |
| 51_L | 87_L | 1.662 | 1.00 |
| 649_N | 685_L | 1.656 | 1.00 |
| 609_L | 644_V | 1.649 | 1.00 |
| 687_S | 690_Y | 1.647 | 1.00 |
| 590_I | 655_K | 1.638 | 1.00 |
| 586_H | 732_H | 1.636 | 1.00 |
| 399_N | 476_M | 1.636 | 1.00 |
| 615_D | 618_S | 1.631 | 1.00 |
| 638_V | 650_L | 1.620 | 1.00 |
| 735_A | 738_T | 1.616 | 1.00 |
| 669_Y | 673_T | 1.612 | 1.00 |
| 589_F | 593_A | 1.610 | 1.00 |
| 178_F | 248_L | 1.608 | 1.00 |
| 738_T | 746_F | 1.606 | 1.00 |
| 550_Q | 563_S | 1.601 | 1.00 |
| 604_F | 607_E | 1.596 | 1.00 |
| 591_D | 595_R | 1.596 | 1.00 |
| 457_G | 461_I | 1.594 | 1.00 |
| 390_W | 540_M | 1.593 | 1.00 |
| 730_I | 738_T | 1.591 | 1.00 |
| 185_H | 280_D | 1.587 | 1.00 |
| 633_G | 712_T | 1.586 | 1.00 |
| 381_T | 438_K | 1.579 | 1.00 |
| 377_A | 443_Q | 1.573 | 1.00 |
| 354_T | 538_E | 1.569 | 1.00 |
| 589_F | 651_M | 1.567 | 1.00 |
| 146_L | 150_P | 1.565 | 1.00 |
| 737_N | 741_D | 1.563 | 1.00 |
| 734_A | 738_T | 1.561 | 1.00 |
| 424_L | 460_F | 1.560 | 1.00 |
| 558_N | 561_R | 1.558 | 1.00 |
| 734_A | 742_I | 1.555 | 1.00 |
| 384_G | 398_I | 1.553 | 1.00 |
| 69_N | 72_A | 1.553 | 1.00 |
| 512_N | 525_W | 1.553 | 1.00 |
| 321_Y | 449_G | 1.552 | 1.00 |
| 225_R | 228_E | 1.551 | 1.00 |
| 741_D | 745_K | 1.548 | 1.00 |
| 382_V | 548_L | 1.547 | 1.00 |
| 47_V | 113_V | 1.546 | 1.00 |
| 319_K | 463_D | 1.537 | 1.00 |
| 661_V | 679_S | 1.535 | 1.00 |
| 600_V | 603_V | 1.534 | 1.00 |
| 741_D | 746_F | 1.530 | 1.00 |
| 649_N | 653_V | 1.523 | 1.00 |
| 444_S | 448_D | 1.522 | 1.00 |
| 602_V | 647_I | 1.519 | 1.00 |
| 630_I | 636_V | 1.518 | 1.00 |
| 343_E | 536_V | 1.517 | 1.00 |
| 673_T | 677_E | 1.515 | 1.00 |
| 180_K | 288_P | 1.515 | 1.00 |
| 318_A | 454_H | 1.514 | 1.00 |
| 588_L | 736_S | 1.513 | 1.00 |
| 366_R | 370_D | 1.507 | 1.00 |
| 365_R | 516_E | 1.507 | 1.00 |
| 469_Q | 472_P | 1.506 | 1.00 |
| 674_Y | 678_N | 1.506 | 1.00 |
| 104_T | 107_E | 1.502 | 1.00 |
| 316_E | 320_A | 1.501 | 1.00 |
| 545_V | 549_D | 1.499 | 1.00 |
| 186_P | 298_T | 1.494 | 1.00 |
| 590_I | 618_S | 1.491 | 1.00 |
| 707_A | 711_D | 1.487 | 1.00 |
| 352_L | 529_E | 1.484 | 1.00 |
| 239_P | 242_Q | 1.471 | 1.00 |
| 640_A | 649_N | 1.470 | 1.00 |
| 323_T | 463_D | 1.470 | 1.00 |
| 170_D | 173_R | 1.469 | 1.00 |
| 320_A | 324_N | 1.469 | 1.00 |
| 169_L | 258_Y | 1.469 | 1.00 |
| 678_N | 724_R | 1.468 | 1.00 |
| 174_L | 260_L | 1.466 | 1.00 |
| 554_Q | 557_I | 1.465 | 1.00 |
| 677_E | 724_R | 1.465 | 1.00 |
| 742_I | 745_K | 1.464 | 1.00 |
| 319_K | 323_T | 1.459 | 1.00 |
| 538_E | 542_T | 1.458 | 1.00 |
| 360_K | 549_D | 1.458 | 1.00 |
| 739_I | 743_N | 1.457 | 1.00 |
| 388_A | 429_M | 1.446 | 1.00 |
| 602_V | 644_V | 1.445 | 1.00 |
| 57_K | 86_A | 1.445 | 1.00 |
| 427_M | 442_T | 1.436 | 1.00 |
| 645_E | 687_S | 1.434 | 1.00 |
| 192_I | 268_L | 1.432 | 1.00 |
| 383_Y | 512_N | 1.430 | 1.00 |
| 352_L | 534_A | 1.428 | 1.00 |
| 738_T | 743_N | 1.420 | 1.00 |
| 543_Q | 564_Q | 1.418 | 1.00 |
| 171_I | 250_A | 1.404 | 0.99 |
| 144_Y | 149_N | 1.401 | 0.99 |
| 157_I | 162_Y | 1.401 | 0.99 |
| 600_V | 610_T | 1.400 | 0.99 |
| 215_V | 255_E | 1.399 | 0.99 |
| 65_G | 68_D | 1.385 | 0.99 |
| 583_A | 587_Q | 1.384 | 0.99 |
| 659_A | 712_T | 1.382 | 0.99 |
| 69_N | 103_P | 1.382 | 0.99 |
| 586_H | 707_A | 1.378 | 0.99 |
| 229_E | 251_F | 1.376 | 0.99 |
| 730_I | 734_A | 1.374 | 0.99 |
| 735_A | 742_I | 1.374 | 0.99 |
| 46_P | 49_Q | 1.371 | 0.99 |
| 459_E | 462_R | 1.367 | 0.99 |
| 679_S | 717_Y | 1.361 | 0.99 |
| 539_S | 543_Q | 1.354 | 0.99 |
| 44_R | 66_I | 1.353 | 0.99 |
| 588_L | 740_Q | 1.353 | 0.99 |
| 527_Y | 537_M | 1.351 | 0.99 |
| 367_L | 552_V | 1.351 | 0.99 |
| 55_W | 105_I | 1.349 | 0.99 |
| 430_D | 433_Q | 1.349 | 0.99 |
| 145_M | 148_R | 1.341 | 0.99 |
| 180_K | 284_Y | 1.340 | 0.99 |
| 737_N | 745_K | 1.325 | 0.99 |
| 164_E | 259_L | 1.322 | 0.99 |
| 455_Y | 463_D | 1.319 | 0.99 |
| 212_E | 249_K | 1.317 | 0.99 |
| 428_D | 433_Q | 1.315 | 0.99 |
| 382_V | 551_L | 1.314 | 0.99 |
| 324_N | 327_P | 1.311 | 0.99 |
| 381_T | 442_T | 1.310 | 0.99 |
| 363_K | 553_E | 1.308 | 0.99 |
| 385_E | 438_K | 1.308 | 0.99 |
| 337_L | 472_P | 1.308 | 0.99 |
| 363_K | 549_D | 1.298 | 0.99 |
| 742_I | 746_F | 1.297 | 0.99 |
| 641_K | 687_S | 1.296 | 0.99 |
| 568_A | 573_Y | 1.296 | 0.99 |
| 299_F | 302_Y | 1.295 | 0.99 |
| 66_I | 105_I | 1.291 | 0.99 |
| 157_I | 160_Q | 1.289 | 0.99 |
| 714_Y | 730_I | 1.287 | 0.99 |
| 190_A | 200_I | 1.285 | 0.99 |
| 213_S | 252_Q | 1.281 | 0.99 |
| 147_G | 150_P | 1.280 | 0.99 |
| 233_M | 236_H | 1.278 | 0.99 |
| 481_T | 484_L | 1.272 | 0.99 |
| 45_N | 66_I | 1.268 | 0.99 |
| 375_P | 521_L | 1.266 | 0.99 |
| 533_D | 536_V | 1.263 | 0.99 |
| 429_M | 433_Q | 1.262 | 0.99 |
| 669_Y | 693_M | 1.260 | 0.99 |
| 357_T | 360_K | 1.259 | 0.99 |
| 610_T | 613_E | 1.249 | 0.99 |
| 357_T | 549_D | 1.249 | 0.99 |
| 250_A | 260_L | 1.246 | 0.98 |
| 386_V | 544_F | 1.242 | 0.98 |
| 532_F | 537_M | 1.242 | 0.98 |
| 589_F | 615_D | 1.241 | 0.98 |
| 173_R | 288_P | 1.241 | 0.98 |
| 334_S | 397_A | 1.234 | 0.98 |
| 568_A | 572_Q | 1.233 | 0.98 |
| 233_M | 263_R | 1.233 | 0.98 |
| 607_E | 617_R | 1.227 | 0.98 |
| 233_M | 249_K | 1.224 | 0.98 |
| 11_V | 14_I | 1.223 | 0.98 |
| 380_C | 398_I | 1.216 | 0.98 |
| 168_E | 256_H | 1.213 | 0.98 |
| 371_K | 440_E | 1.212 | 0.98 |
| 624_F | 627_S | 1.210 | 0.98 |
| 321_Y | 325_K | 1.207 | 0.98 |
| 385_E | 547_L | 1.207 | 0.98 |
| 603_V | 608_W | 1.203 | 0.98 |
| 145_M | 150_P | 1.200 | 0.98 |
| 667_H | 672_Q | 1.198 | 0.98 |
| 686_D | 690_Y | 1.192 | 0.98 |
| 39_P | 67_H | 1.191 | 0.98 |
| 607_E | 645_E | 1.187 | 0.98 |
| 140_V | 143_A | 1.187 | 0.98 |
| 432_K | 560_L | 1.186 | 0.98 |
| 457_G | 460_F | 1.181 | 0.98 |
| 670_E | 674_Y | 1.175 | 0.98 |
| 557_I | 561_R | 1.172 | 0.98 |
| 98_D | 111_R | 1.172 | 0.98 |
| 630_I | 656_A | 1.172 | 0.98 |
| 325_K | 448_D | 1.171 | 0.98 |
| 279_Q | 283_H | 1.169 | 0.97 |
| 593_A | 615_D | 1.168 | 0.97 |
| 566_D | 570_I | 1.164 | 0.97 |
| 590_I | 619_N | 1.160 | 0.97 |
| 446_L | 450_L | 1.159 | 0.97 |
| 670_E | 724_R | 1.158 | 0.97 |
| 628_R | 683_I | 1.158 | 0.97 |
| 144_Y | 150_P | 1.157 | 0.97 |
| 228_E | 232_R | 1.155 | 0.97 |
| 302_Y | 305_I | 1.154 | 0.97 |
| 67_H | 106_A | 1.152 | 0.97 |
| 547_L | 562_I | 1.151 | 0.97 |
| 730_I | 741_D | 1.147 | 0.97 |
| 131_L | 135_F | 1.144 | 0.97 |
| 460_F | 463_D | 1.142 | 0.97 |
| 618_S | 652_A | 1.140 | 0.97 |
| 364_L | 378_L | 1.135 | 0.97 |
| 50_K | 53_Q | 1.135 | 0.97 |
| 373_V | 439_V | 1.132 | 0.97 |
| 572_Q | 575_A | 1.131 | 0.97 |
| 459_E | 463_D | 1.130 | 0.97 |
| 248_L | 260_L | 1.128 | 0.97 |
| 169_L | 174_L | 1.126 | 0.97 |
| 373_V | 377_A | 1.124 | 0.97 |
| 193_L | 199_Q | 1.123 | 0.97 |
| 546_E | 550_Q | 1.121 | 0.97 |
| 654_L | 713_A | 1.117 | 0.96 |
| 383_Y | 480_F | 1.116 | 0.96 |
| 170_D | 285_Y | 1.115 | 0.96 |
| 636_V | 658_A | 1.115 | 0.96 |
| 215_V | 252_Q | 1.113 | 0.96 |
| 607_E | 644_V | 1.112 | 0.96 |
| 617_R | 648_I | 1.111 | 0.96 |
| 102_Y | 111_R | 1.111 | 0.96 |
| 633_G | 731_T | 1.110 | 0.96 |
| 54_T | 87_L | 1.110 | 0.96 |
| 637_G | 676_L | 1.108 | 0.96 |
| 226_L | 259_L | 1.107 | 0.96 |
| 573_Y | 576_T | 1.105 | 0.96 |
| 361_W | 521_L | 1.104 | 0.96 |
| 240_L | 267_L | 1.103 | 0.96 |
| 306_Y | 310_K | 1.102 | 0.96 |
| 570_I | 574_N | 1.100 | 0.96 |
| 585_L | 654_L | 1.096 | 0.96 |
| 617_R | 620_Q | 1.093 | 0.96 |
| 354_T | 541_F | 1.093 | 0.96 |
| 712_T | 731_T | 1.088 | 0.96 |
| 252_Q | 258_Y | 1.086 | 0.96 |
| 614_L | 651_M | 1.085 | 0.96 |
| 175_S | 208_E | 1.084 | 0.96 |
| 187_M | 190_A | 1.077 | 0.95 |
| 586_H | 654_L | 1.075 | 0.95 |
| 155_S | 158_S | 1.074 | 0.95 |
| 350_Q | 530_E | 1.073 | 0.95 |
| 512_N | 523_V | 1.072 | 0.95 |
| 284_Y | 288_P | 1.071 | 0.95 |
| 367_L | 371_K | 1.070 | 0.95 |
| 644_V | 648_I | 1.068 | 0.95 |
| 455_Y | 460_F | 1.056 | 0.95 |
| 134_P | 197_K | 1.055 | 0.95 |
| 363_K | 552_V | 1.055 | 0.95 |
| 566_D | 571_D | 1.053 | 0.95 |
| 639_L | 672_Q | 1.051 | 0.94 |
| 662_P | 716_I | 1.050 | 0.94 |
| 176_R | 288_P | 1.047 | 0.94 |
| 379_L | 521_L | 1.046 | 0.94 |
| 587_Q | 590_I | 1.045 | 0.94 |
| 48_E | 66_I | 1.045 | 0.94 |
| 321_Y | 448_D | 1.044 | 0.94 |
| 388_A | 391_S | 1.044 | 0.94 |
| 159_P | 162_Y | 1.042 | 0.94 |
| 437_T | 440_E | 1.042 | 0.94 |
| 361_W | 365_R | 1.040 | 0.94 |
| 734_A | 739_I | 1.038 | 0.94 |
| 251_F | 261_C | 1.036 | 0.94 |
| 354_T | 534_A | 1.034 | 0.94 |
| 328_D | 426_D | 1.032 | 0.94 |
| 511_D | 526_N | 1.027 | 0.94 |
| 254_Q | 257_T | 1.025 | 0.93 |
| 659_A | 715_V | 1.025 | 0.93 |
| 377_A | 447_L | 1.022 | 0.93 |
| 639_L | 663_I | 1.020 | 0.93 |
| 380_C | 384_G | 1.017 | 0.93 |
| 192_I | 267_L | 1.017 | 0.93 |
| 380_C | 446_L | 1.017 | 0.93 |
| 175_S | 211_V | 1.014 | 0.93 |
| 142_T | 307_D | 1.013 | 0.93 |
| 148_R | 194_P | 1.010 | 0.93 |
| 619_N | 702_D | 1.008 | 0.93 |
| 614_L | 648_I | 1.008 | 0.93 |
| 456_D | 459_E | 1.007 | 0.93 |
| 626_Q | 707_A | 1.007 | 0.93 |
| 596_T | 612_S | 1.007 | 0.93 |
| 441_Q | 445_T | 1.005 | 0.93 |
| 640_A | 685_L | 1.005 | 0.93 |
| 88_R | 93_K | 1.005 | 0.93 |
| 361_W | 375_P | 1.004 | 0.93 |
| 191_V | 244_P | 1.004 | 0.93 |
| 137_L | 267_L | 1.004 | 0.93 |
| 373_V | 378_L | 1.002 | 0.92 |
| 138_T | 299_F | 1.000 | 0.92 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 2vsqA | 1 | 0.811 | 100 | 0.325 | Contact Map |
| 2jgpA | 1 | 0.681 | 100 | 0.444 | Contact Map |
| 4tx3B | 1 | 0.5724 | 100 | 0.584 | Contact Map |
| 1l5aA | 3 | 0.5617 | 100 | 0.584 | Contact Map |
| 4znmA | 2 | 0 | 100 | 0.591 | Contact Map |
| 4jn3A | 2 | 0.567 | 100 | 0.593 | Contact Map |
| 4hvmA | 4 | 0.508 | 100 | 0.598 | Contact Map |
| 2xhgA | 1 | 0.567 | 100 | 0.621 | Contact Map |
| 1q9jA | 2 | 0.4906 | 100 | 0.68 | Contact Map |
| 3fotA | 1 | 0.5777 | 100 | 0.701 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)