| gpcr_1e-10 |
| ID: | 1501449622 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 292 (285) | ||
| Sequences: | 18787 (11377.1) | ||
| Seq/Len: | 65.919 | ||
| Nf(neff/√len): | 673.9 |
HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 65.919).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 13_S | 55_T | 4.761 | 1.00 |
| 46_H | 128_W | 4.255 | 1.00 |
| 160_C | 164_C | 3.828 | 1.00 |
| 193_I | 197_K | 3.603 | 1.00 |
| 46_H | 95_M | 3.064 | 1.00 |
| 98_V | 127_A | 2.618 | 1.00 |
| 196_A | 200_F | 2.557 | 1.00 |
| 104_S | 197_K | 2.543 | 1.00 |
| 76_L | 80_V | 2.429 | 1.00 |
| 190_I | 194_C | 2.413 | 1.00 |
| 104_S | 109_R | 2.382 | 1.00 |
| 75_E | 79_K | 2.254 | 1.00 |
| 197_K | 200_F | 2.207 | 1.00 |
| 50_A | 91_A | 2.204 | 1.00 |
| 87_F | 132_S | 2.157 | 1.00 |
| 118_K | 122_S | 2.102 | 1.00 |
| 104_S | 108_T | 2.086 | 1.00 |
| 91_A | 131_S | 2.077 | 1.00 |
| 232_M | 290_F | 2.043 | 1.00 |
| 98_V | 123_M | 2.025 | 1.00 |
| 83_Y | 87_F | 1.981 | 1.00 |
| 53_L | 88_S | 1.961 | 1.00 |
| 78_C | 160_C | 1.918 | 1.00 |
| 71_W | 74_G | 1.908 | 1.00 |
| 51_N | 283_D | 1.906 | 1.00 |
| 42_L | 124_V | 1.900 | 1.00 |
| 95_M | 124_V | 1.889 | 1.00 |
| 109_R | 112_A | 1.878 | 1.00 |
| 249_V | 253_W | 1.859 | 1.00 |
| 18_A | 22_L | 1.849 | 1.00 |
| 244_W | 276_A | 1.846 | 1.00 |
| 108_T | 201_T | 1.832 | 1.00 |
| 46_H | 124_V | 1.831 | 1.00 |
| 201_T | 204_R | 1.822 | 1.00 |
| 104_S | 194_C | 1.802 | 1.00 |
| 197_K | 201_T | 1.782 | 1.00 |
| 239_S | 286_I | 1.779 | 1.00 |
| 21_L | 45_K | 1.773 | 1.00 |
| 200_F | 204_R | 1.711 | 1.00 |
| 123_M | 127_A | 1.705 | 1.00 |
| 122_S | 126_L | 1.704 | 1.00 |
| 102_D | 106_A | 1.659 | 1.00 |
| 188_L | 241_T | 1.640 | 1.00 |
| 24_L | 45_K | 1.620 | 1.00 |
| 97_V | 194_C | 1.591 | 1.00 |
| 45_K | 49_L | 1.569 | 1.00 |
| 42_L | 121_Q | 1.549 | 1.00 |
| 121_Q | 125_G | 1.546 | 1.00 |
| 43_L | 120_G | 1.538 | 1.00 |
| 95_M | 128_W | 1.538 | 1.00 |
| 51_N | 92_P | 1.536 | 1.00 |
| 101_L | 194_C | 1.534 | 1.00 |
| 97_V | 190_I | 1.534 | 1.00 |
| 268_V | 272_F | 1.518 | 1.00 |
| 83_Y | 139_L | 1.517 | 1.00 |
| 283_D | 287_Y | 1.515 | 1.00 |
| 192_L | 196_A | 1.491 | 1.00 |
| 118_K | 121_Q | 1.477 | 1.00 |
| 94_F | 187_P | 1.439 | 1.00 |
| 136_G | 140_Y | 1.438 | 1.00 |
| 199_I | 203_T | 1.426 | 1.00 |
| 105_L | 109_R | 1.421 | 1.00 |
| 54_E | 280_P | 1.418 | 1.00 |
| 91_A | 128_W | 1.417 | 1.00 |
| 278_L | 282_F | 1.403 | 0.99 |
| 50_A | 92_P | 1.400 | 0.99 |
| 100_S | 195_N | 1.396 | 0.99 |
| 14_A | 52_L | 1.386 | 0.99 |
| 48_T | 51_N | 1.382 | 0.99 |
| 104_S | 193_I | 1.370 | 0.99 |
| 43_L | 99_I | 1.363 | 0.99 |
| 74_G | 77_L | 1.362 | 0.99 |
| 96_M | 191_M | 1.354 | 0.99 |
| 106_A | 110_P | 1.353 | 0.99 |
| 91_A | 95_M | 1.316 | 0.99 |
| 50_A | 128_W | 1.314 | 0.99 |
| 15_T | 18_A | 1.300 | 0.99 |
| 116_N | 119_V | 1.293 | 0.99 |
| 47_L | 95_M | 1.279 | 0.99 |
| 16_F | 281_C | 1.274 | 0.99 |
| 178_F | 182_C | 1.273 | 0.99 |
| 60_P | 63_G | 1.267 | 0.99 |
| 119_V | 122_S | 1.267 | 0.99 |
| 199_I | 230_L | 1.259 | 0.99 |
| 18_A | 52_L | 1.236 | 0.98 |
| 227_L | 231_K | 1.225 | 0.98 |
| 101_L | 105_L | 1.223 | 0.98 |
| 36_K | 41_K | 1.222 | 0.98 |
| 21_L | 52_L | 1.209 | 0.98 |
| 43_L | 95_M | 1.208 | 0.98 |
| 16_F | 285_L | 1.191 | 0.98 |
| 94_F | 130_L | 1.189 | 0.98 |
| 106_A | 113_L | 1.183 | 0.98 |
| 75_E | 143_R | 1.167 | 0.97 |
| 46_H | 50_A | 1.163 | 0.97 |
| 90_Y | 183_L | 1.157 | 0.97 |
| 267_P | 271_F | 1.153 | 0.97 |
| 100_S | 194_C | 1.149 | 0.97 |
| 257_D | 260_M | 1.146 | 0.97 |
| 241_T | 245_T | 1.143 | 0.97 |
| 245_T | 249_V | 1.142 | 0.97 |
| 14_A | 55_T | 1.141 | 0.97 |
| 264_L | 268_V | 1.131 | 0.97 |
| 97_V | 191_M | 1.129 | 0.97 |
| 229_T | 232_M | 1.123 | 0.97 |
| 43_L | 102_D | 1.121 | 0.97 |
| 20_F | 24_L | 1.120 | 0.96 |
| 106_A | 111_L | 1.108 | 0.96 |
| 199_I | 202_L | 1.105 | 0.96 |
| 21_L | 48_T | 1.104 | 0.96 |
| 10_F | 55_T | 1.103 | 0.96 |
| 87_F | 135_A | 1.098 | 0.96 |
| 205_V | 209_D | 1.098 | 0.96 |
| 13_S | 281_C | 1.097 | 0.96 |
| 131_S | 135_A | 1.094 | 0.96 |
| 19_S | 23_K | 1.094 | 0.96 |
| 112_A | 115_S | 1.093 | 0.96 |
| 235_A | 290_F | 1.073 | 0.95 |
| 273_F | 277_F | 1.069 | 0.95 |
| 64_M | 73_A | 1.066 | 0.95 |
| 250_L | 268_V | 1.064 | 0.95 |
| 43_L | 98_V | 1.063 | 0.95 |
| 226_R | 230_L | 1.063 | 0.95 |
| 38_S | 41_K | 1.062 | 0.95 |
| 250_L | 272_F | 1.059 | 0.95 |
| 17_N | 20_F | 1.057 | 0.95 |
| 279_N | 283_D | 1.056 | 0.95 |
| 41_K | 45_K | 1.044 | 0.94 |
| 10_F | 14_A | 1.041 | 0.94 |
| 46_H | 91_A | 1.031 | 0.94 |
| 108_T | 197_K | 1.027 | 0.94 |
| 117_S | 121_Q | 1.026 | 0.94 |
| 39_R | 120_G | 1.019 | 0.93 |
| 94_F | 131_S | 1.018 | 0.93 |
| 76_L | 79_K | 1.014 | 0.93 |
| 93_A | 187_P | 1.012 | 0.93 |
| 20_F | 284_P | 1.008 | 0.93 |
| 239_S | 282_F | 1.005 | 0.93 |
| 138_Q | 141_I | 1.003 | 0.93 |
| 184_F | 188_L | 1.003 | 0.93 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 4iarA | 1 | 0.8938 | 100 | 0.087 | Contact Map |
| 4pxzA | 1 | 0.9315 | 100 | 0.098 | Contact Map |
| 2rh1A | 1 | 0.9452 | 100 | 0.098 | Contact Map |
| 3uonA | 1 | 0.9384 | 100 | 0.108 | Contact Map |
| 3oduA | 2 | 0.9418 | 100 | 0.11 | Contact Map |
| 4dklA | 1 | 0.9521 | 100 | 0.111 | Contact Map |
| 3vw7A | 1 | 0.9452 | 100 | 0.113 | Contact Map |
| 3pblA | 2 | 0.911 | 100 | 0.116 | Contact Map |
| 4grvA | 1 | 0.9623 | 100 | 0.116 | Contact Map |
| 4bvnA | 1 | 0.9212 | 100 | 0.118 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)