| gpcr_1e-4 |
| ID: | 1501449595 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 292 (285) | ||
| Sequences: | 19416 (11581.3) | ||
| Seq/Len: | 68.126 | ||
| Nf(neff/√len): | 686.0 |
HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 68.126).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 13_S | 55_T | 4.239 | 1.00 |
| 46_H | 128_W | 4.238 | 1.00 |
| 160_C | 164_C | 3.898 | 1.00 |
| 193_I | 197_K | 3.641 | 1.00 |
| 46_H | 95_M | 3.019 | 1.00 |
| 104_S | 197_K | 2.656 | 1.00 |
| 196_A | 200_F | 2.499 | 1.00 |
| 98_V | 127_A | 2.498 | 1.00 |
| 76_L | 80_V | 2.450 | 1.00 |
| 50_A | 91_A | 2.359 | 1.00 |
| 190_I | 194_C | 2.238 | 1.00 |
| 104_S | 109_R | 2.149 | 1.00 |
| 83_Y | 87_F | 2.104 | 1.00 |
| 75_E | 79_K | 2.093 | 1.00 |
| 197_K | 200_F | 2.072 | 1.00 |
| 232_M | 290_F | 2.034 | 1.00 |
| 118_K | 122_S | 2.026 | 1.00 |
| 104_S | 194_C | 2.014 | 1.00 |
| 91_A | 131_S | 2.010 | 1.00 |
| 104_S | 108_T | 1.999 | 1.00 |
| 51_N | 283_D | 1.990 | 1.00 |
| 109_R | 112_A | 1.979 | 1.00 |
| 53_L | 88_S | 1.971 | 1.00 |
| 87_F | 132_S | 1.958 | 1.00 |
| 108_T | 201_T | 1.950 | 1.00 |
| 244_W | 276_A | 1.924 | 1.00 |
| 21_L | 45_K | 1.890 | 1.00 |
| 46_H | 124_V | 1.857 | 1.00 |
| 98_V | 123_M | 1.848 | 1.00 |
| 200_F | 204_R | 1.844 | 1.00 |
| 197_K | 201_T | 1.804 | 1.00 |
| 122_S | 126_L | 1.795 | 1.00 |
| 239_S | 286_I | 1.762 | 1.00 |
| 71_W | 74_G | 1.761 | 1.00 |
| 78_C | 160_C | 1.748 | 1.00 |
| 42_L | 124_V | 1.747 | 1.00 |
| 97_V | 194_C | 1.720 | 1.00 |
| 95_M | 124_V | 1.702 | 1.00 |
| 18_A | 22_L | 1.640 | 1.00 |
| 201_T | 204_R | 1.637 | 1.00 |
| 123_M | 127_A | 1.601 | 1.00 |
| 268_V | 272_F | 1.600 | 1.00 |
| 101_L | 194_C | 1.598 | 1.00 |
| 188_L | 241_T | 1.576 | 1.00 |
| 121_Q | 125_G | 1.573 | 1.00 |
| 45_K | 49_L | 1.558 | 1.00 |
| 249_V | 253_W | 1.542 | 1.00 |
| 102_D | 106_A | 1.533 | 1.00 |
| 106_A | 110_P | 1.510 | 1.00 |
| 97_V | 190_I | 1.499 | 1.00 |
| 91_A | 95_M | 1.494 | 1.00 |
| 95_M | 128_W | 1.482 | 1.00 |
| 74_G | 77_L | 1.458 | 1.00 |
| 54_E | 280_P | 1.441 | 1.00 |
| 96_M | 191_M | 1.439 | 1.00 |
| 51_N | 92_P | 1.431 | 1.00 |
| 101_L | 105_L | 1.426 | 1.00 |
| 43_L | 120_G | 1.424 | 1.00 |
| 50_A | 92_P | 1.418 | 1.00 |
| 83_Y | 139_L | 1.408 | 1.00 |
| 91_A | 128_W | 1.403 | 0.99 |
| 283_D | 287_Y | 1.400 | 0.99 |
| 42_L | 121_Q | 1.385 | 0.99 |
| 94_F | 130_L | 1.381 | 0.99 |
| 105_L | 109_R | 1.376 | 0.99 |
| 50_A | 128_W | 1.375 | 0.99 |
| 100_S | 195_N | 1.375 | 0.99 |
| 131_S | 135_A | 1.373 | 0.99 |
| 278_L | 282_F | 1.371 | 0.99 |
| 94_F | 187_P | 1.366 | 0.99 |
| 48_T | 51_N | 1.356 | 0.99 |
| 24_L | 45_K | 1.340 | 0.99 |
| 199_I | 230_L | 1.321 | 0.99 |
| 14_A | 52_L | 1.313 | 0.99 |
| 136_G | 140_Y | 1.310 | 0.99 |
| 104_S | 193_I | 1.308 | 0.99 |
| 192_L | 196_A | 1.300 | 0.99 |
| 90_Y | 183_L | 1.288 | 0.99 |
| 50_A | 88_S | 1.286 | 0.99 |
| 118_K | 121_Q | 1.281 | 0.99 |
| 226_R | 230_L | 1.268 | 0.99 |
| 199_I | 203_T | 1.266 | 0.99 |
| 245_T | 249_V | 1.263 | 0.99 |
| 43_L | 99_I | 1.254 | 0.99 |
| 87_F | 135_A | 1.253 | 0.99 |
| 239_S | 282_F | 1.222 | 0.98 |
| 15_T | 18_A | 1.215 | 0.98 |
| 94_F | 97_V | 1.207 | 0.98 |
| 93_A | 240_F | 1.202 | 0.98 |
| 19_S | 23_K | 1.201 | 0.98 |
| 267_P | 271_F | 1.201 | 0.98 |
| 178_F | 182_C | 1.189 | 0.98 |
| 116_N | 119_V | 1.185 | 0.98 |
| 100_S | 191_M | 1.180 | 0.98 |
| 47_L | 95_M | 1.179 | 0.98 |
| 241_T | 245_T | 1.173 | 0.98 |
| 43_L | 95_M | 1.170 | 0.97 |
| 43_L | 102_D | 1.161 | 0.97 |
| 112_A | 115_S | 1.160 | 0.97 |
| 22_L | 26_K | 1.147 | 0.97 |
| 257_D | 260_M | 1.144 | 0.97 |
| 229_T | 233_T | 1.143 | 0.97 |
| 229_T | 232_M | 1.133 | 0.97 |
| 250_L | 268_V | 1.131 | 0.97 |
| 195_N | 198_I | 1.123 | 0.97 |
| 105_L | 113_L | 1.116 | 0.96 |
| 21_L | 48_T | 1.116 | 0.96 |
| 46_H | 50_A | 1.112 | 0.96 |
| 205_V | 209_D | 1.110 | 0.96 |
| 42_L | 46_H | 1.107 | 0.96 |
| 86_L | 135_A | 1.105 | 0.96 |
| 75_E | 143_R | 1.098 | 0.96 |
| 16_F | 281_C | 1.094 | 0.96 |
| 127_A | 131_S | 1.094 | 0.96 |
| 100_S | 194_C | 1.082 | 0.96 |
| 106_A | 111_L | 1.080 | 0.95 |
| 273_F | 277_F | 1.077 | 0.95 |
| 138_Q | 141_I | 1.077 | 0.95 |
| 280_P | 284_P | 1.076 | 0.95 |
| 94_F | 127_A | 1.073 | 0.95 |
| 60_P | 63_G | 1.068 | 0.95 |
| 264_L | 268_V | 1.064 | 0.95 |
| 18_A | 52_L | 1.062 | 0.95 |
| 279_N | 283_D | 1.056 | 0.95 |
| 10_F | 14_A | 1.047 | 0.94 |
| 43_L | 98_V | 1.046 | 0.94 |
| 36_K | 41_K | 1.037 | 0.94 |
| 106_A | 113_L | 1.033 | 0.94 |
| 94_F | 131_S | 1.033 | 0.94 |
| 199_I | 202_L | 1.031 | 0.94 |
| 13_S | 281_C | 1.029 | 0.94 |
| 21_L | 52_L | 1.027 | 0.94 |
| 76_L | 79_K | 1.025 | 0.93 |
| 93_A | 187_P | 1.019 | 0.93 |
| 126_L | 130_L | 1.013 | 0.93 |
| 122_S | 125_G | 1.011 | 0.93 |
| 83_Y | 135_A | 1.010 | 0.93 |
| 64_M | 73_A | 1.010 | 0.93 |
| 107_I | 110_P | 1.007 | 0.93 |
| 90_Y | 131_S | 1.006 | 0.93 |
| 97_V | 191_M | 1.005 | 0.93 |
| 20_F | 24_L | 1.004 | 0.93 |
| 108_T | 197_K | 1.002 | 0.92 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 4iarA | 1 | 0.8938 | 100 | 0.091 | Contact Map |
| 2rh1A | 1 | 0.9452 | 100 | 0.092 | Contact Map |
| 4pxzA | 1 | 0.9315 | 100 | 0.096 | Contact Map |
| 3uonA | 1 | 0.9384 | 100 | 0.101 | Contact Map |
| 3oduA | 2 | 0.9418 | 100 | 0.106 | Contact Map |
| 4dklA | 1 | 0.9521 | 100 | 0.106 | Contact Map |
| 3vw7A | 1 | 0.9452 | 100 | 0.107 | Contact Map |
| 4grvA | 1 | 0.9623 | 100 | 0.11 | Contact Map |
| 3rzeA | 1 | 0.9144 | 100 | 0.112 | Contact Map |
| 3emlA | 1 | 0.9384 | 100 | 0.113 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)