| Glucose-6-phosphate 1-dehydrogenase e coli |
| ID: | 1498046556 |
Query Sequence |
Download Alignment We filter this alignment to remove positions that have > 75% gaps before running GREMLIN. |
| Length: | 491 (484) | ||
| Sequences: | 3033 (1636.6) | ||
| Seq/Len: | 6.267 | ||
| Nf(neff/√len): | 74.4 |
HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied
Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 6.267).
Sequences per length for each position, AFTER the coverage/gap filter was applied
Residue pairs sorted by coevolution strength:
Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.
| i | j | Scaled score | Prob |
| 263_E | 266_K | 4.601 | 1.00 |
| 272_R | 324_D | 4.468 | 1.00 |
| 54_D | 57_A | 3.531 | 1.00 |
| 119_M | 127_I | 3.375 | 1.00 |
| 271_L | 448_W | 3.310 | 1.00 |
| 209_V | 333_F | 3.228 | 1.00 |
| 207_D | 485_R | 3.216 | 1.00 |
| 17_A | 58_Y | 3.156 | 1.00 |
| 225_Y | 229_A | 3.121 | 1.00 |
| 248_A | 333_F | 3.092 | 1.00 |
| 79_W | 83_S | 3.057 | 1.00 |
| 262_D | 438_R | 2.935 | 1.00 |
| 464_K | 475_A | 2.884 | 1.00 |
| 63_R | 67_E | 2.842 | 1.00 |
| 226_F | 231_Q | 2.829 | 1.00 |
| 450_D | 454_E | 2.741 | 1.00 |
| 285_R | 456_W | 2.678 | 1.00 |
| 444_E | 447_K | 2.620 | 1.00 |
| 152_S | 155_T | 2.551 | 1.00 |
| 55_K | 59_T | 2.505 | 1.00 |
| 163_V | 172_V | 2.493 | 1.00 |
| 144_V | 428_T | 2.442 | 1.00 |
| 352_F | 372_L | 2.269 | 1.00 |
| 55_K | 87_D | 2.247 | 1.00 |
| 231_Q | 337_T | 2.228 | 1.00 |
| 286_G | 465_P | 2.222 | 1.00 |
| 157_Q | 443_E | 2.204 | 1.00 |
| 300_G | 303_E | 2.173 | 1.00 |
| 95_D | 98_A | 2.114 | 1.00 |
| 143_V | 167_F | 2.107 | 1.00 |
| 347_E | 375_R | 2.085 | 1.00 |
| 145_M | 163_V | 2.073 | 1.00 |
| 74_I | 77_G | 2.072 | 1.00 |
| 168_E | 171_Q | 2.064 | 1.00 |
| 114_I | 428_T | 2.063 | 1.00 |
| 227_D | 313_T | 2.062 | 1.00 |
| 289_T | 465_P | 2.021 | 1.00 |
| 52_D | 90_N | 2.010 | 1.00 |
| 149_L | 159_I | 1.998 | 1.00 |
| 342_P | 471_W | 1.997 | 1.00 |
| 151_T | 155_T | 1.995 | 1.00 |
| 60_K | 64_E | 1.979 | 1.00 |
| 236_I | 317_V | 1.971 | 1.00 |
| 272_R | 322_D | 1.965 | 1.00 |
| 120_P | 123_T | 1.962 | 1.00 |
| 262_D | 440_D | 1.959 | 1.00 |
| 153_L | 157_Q | 1.949 | 1.00 |
| 103_G | 107_D | 1.945 | 1.00 |
| 27_L | 62_V | 1.928 | 1.00 |
| 349_V | 371_K | 1.889 | 1.00 |
| 129_K | 165_E | 1.844 | 1.00 |
| 250_S | 327_R | 1.831 | 1.00 |
| 128_C | 163_V | 1.788 | 1.00 |
| 320_R | 483_D | 1.787 | 1.00 |
| 254_D | 259_S | 1.770 | 1.00 |
| 189_A | 399_L | 1.765 | 1.00 |
| 291_G | 298_V | 1.759 | 1.00 |
| 334_Y | 483_D | 1.749 | 1.00 |
| 201_W | 206_I | 1.740 | 1.00 |
| 34_E | 85_R | 1.738 | 1.00 |
| 218_G | 340_R | 1.738 | 1.00 |
| 200_N | 352_F | 1.726 | 1.00 |
| 286_G | 463_P | 1.722 | 1.00 |
| 93_V | 123_T | 1.718 | 1.00 |
| 34_E | 82_L | 1.718 | 1.00 |
| 211_I | 244_L | 1.706 | 1.00 |
| 63_R | 80_D | 1.703 | 1.00 |
| 231_Q | 314_E | 1.696 | 1.00 |
| 160_N | 443_E | 1.695 | 1.00 |
| 236_I | 337_T | 1.688 | 1.00 |
| 283_T | 452_I | 1.683 | 1.00 |
| 20_D | 24_R | 1.663 | 1.00 |
| 76_E | 80_D | 1.655 | 1.00 |
| 190_L | 374_I | 1.655 | 1.00 |
| 22_A | 27_L | 1.653 | 1.00 |
| 209_V | 248_A | 1.637 | 1.00 |
| 474_V | 478_A | 1.622 | 1.00 |
| 213_V | 346_S | 1.605 | 1.00 |
| 271_L | 321_V | 1.603 | 1.00 |
| 129_K | 162_Q | 1.576 | 0.99 |
| 326_W | 356_E | 1.547 | 0.99 |
| 55_K | 105_M | 1.534 | 0.99 |
| 290_A | 303_E | 1.534 | 0.99 |
| 243_I | 348_V | 1.531 | 0.99 |
| 190_L | 382_V | 1.530 | 0.99 |
| 223_W | 306_G | 1.528 | 0.99 |
| 210_E | 351_Y | 1.528 | 0.99 |
| 53_W | 57_A | 1.525 | 0.99 |
| 450_D | 453_T | 1.522 | 0.99 |
| 389_K | 392_G | 1.506 | 0.99 |
| 342_P | 470_T | 1.499 | 0.99 |
| 17_A | 47_G | 1.499 | 0.99 |
| 298_V | 340_R | 1.499 | 0.99 |
| 53_W | 61_V | 1.491 | 0.99 |
| 293_A | 298_V | 1.471 | 0.99 |
| 91_L | 98_A | 1.464 | 0.99 |
| 372_L | 384_I | 1.458 | 0.99 |
| 197_F | 386_V | 1.447 | 0.99 |
| 210_E | 487_W | 1.447 | 0.99 |
| 62_V | 66_L | 1.444 | 0.99 |
| 137_N | 167_F | 1.439 | 0.99 |
| 132_G | 166_Y | 1.434 | 0.99 |
| 112_I | 140_P | 1.429 | 0.99 |
| 302_L | 465_P | 1.428 | 0.99 |
| 48_V | 91_L | 1.425 | 0.99 |
| 207_D | 353_K | 1.419 | 0.99 |
| 122_S | 228_K | 1.418 | 0.99 |
| 97_A | 100_S | 1.411 | 0.98 |
| 241_L | 268_L | 1.410 | 0.98 |
| 89_C | 105_M | 1.406 | 0.98 |
| 262_D | 269_K | 1.398 | 0.98 |
| 478_A | 481_T | 1.392 | 0.98 |
| 290_A | 300_G | 1.386 | 0.98 |
| 22_A | 62_V | 1.386 | 0.98 |
| 336_R | 487_W | 1.383 | 0.98 |
| 87_D | 105_M | 1.379 | 0.98 |
| 101_R | 104_A | 1.372 | 0.98 |
| 208_H | 351_Y | 1.368 | 0.98 |
| 208_H | 485_R | 1.361 | 0.98 |
| 283_T | 319_I | 1.361 | 0.98 |
| 321_V | 448_W | 1.357 | 0.98 |
| 203_N | 353_K | 1.354 | 0.98 |
| 182_E | 389_K | 1.348 | 0.98 |
| 195_S | 199_N | 1.347 | 0.98 |
| 13_V | 106_L | 1.345 | 0.98 |
| 251_P | 326_W | 1.339 | 0.98 |
| 129_K | 133_E | 1.336 | 0.98 |
| 46_I | 105_M | 1.333 | 0.98 |
| 10_C | 429_M | 1.328 | 0.98 |
| 285_R | 314_E | 1.326 | 0.97 |
| 377_Q | 380_E | 1.321 | 0.97 |
| 351_Y | 371_K | 1.320 | 0.97 |
| 316_F | 477_V | 1.317 | 0.97 |
| 128_C | 143_V | 1.315 | 0.97 |
| 275_D | 278_N | 1.312 | 0.97 |
| 62_V | 86_L | 1.301 | 0.97 |
| 340_R | 468_A | 1.298 | 0.97 |
| 474_V | 477_V | 1.297 | 0.97 |
| 153_L | 443_E | 1.295 | 0.97 |
| 232_M | 456_W | 1.292 | 0.97 |
| 265_V | 438_R | 1.288 | 0.97 |
| 80_D | 84_A | 1.267 | 0.96 |
| 34_E | 81_T | 1.264 | 0.96 |
| 235_M | 337_T | 1.262 | 0.96 |
| 81_T | 84_A | 1.260 | 0.96 |
| 250_S | 325_N | 1.249 | 0.96 |
| 245_C | 268_L | 1.240 | 0.96 |
| 241_L | 449_V | 1.235 | 0.96 |
| 301_Y | 307_A | 1.233 | 0.96 |
| 96_T | 130_G | 1.226 | 0.96 |
| 227_D | 308_N | 1.225 | 0.95 |
| 478_A | 482_R | 1.225 | 0.95 |
| 125_G | 162_Q | 1.223 | 0.95 |
| 195_S | 205_T | 1.222 | 0.95 |
| 227_D | 302_L | 1.218 | 0.95 |
| 154_A | 158_E | 1.213 | 0.95 |
| 11_D | 44_R | 1.205 | 0.95 |
| 154_A | 157_Q | 1.203 | 0.95 |
| 14_I | 22_A | 1.202 | 0.95 |
| 66_L | 79_W | 1.197 | 0.95 |
| 49_G | 88_F | 1.196 | 0.95 |
| 161_D | 165_E | 1.194 | 0.95 |
| 316_F | 476_S | 1.192 | 0.95 |
| 18_K | 49_G | 1.190 | 0.94 |
| 283_T | 456_W | 1.185 | 0.94 |
| 213_V | 235_M | 1.185 | 0.94 |
| 96_T | 133_E | 1.182 | 0.94 |
| 198_V | 327_R | 1.178 | 0.94 |
| 78_L | 81_T | 1.174 | 0.94 |
| 407_S | 410_E | 1.173 | 0.94 |
| 182_E | 392_G | 1.169 | 0.94 |
| 25_K | 419_D | 1.167 | 0.94 |
| 349_V | 488_N | 1.167 | 0.94 |
| 199_N | 205_T | 1.165 | 0.94 |
| 48_V | 98_A | 1.164 | 0.94 |
| 158_E | 162_Q | 1.163 | 0.94 |
| 46_I | 89_C | 1.160 | 0.93 |
| 78_L | 82_L | 1.156 | 0.93 |
| 93_V | 127_I | 1.155 | 0.93 |
| 57_A | 60_K | 1.147 | 0.93 |
| 18_K | 51_A | 1.144 | 0.93 |
| 45_I | 86_L | 1.142 | 0.93 |
| 247_I | 350_V | 1.140 | 0.93 |
| 326_W | 329_A | 1.139 | 0.93 |
| 125_G | 158_E | 1.137 | 0.93 |
| 240_L | 335_L | 1.135 | 0.92 |
| 24_R | 235_M | 1.134 | 0.92 |
| 264_K | 441_E | 1.131 | 0.92 |
| 48_V | 124_F | 1.128 | 0.92 |
| 212_T | 336_R | 1.124 | 0.92 |
| 386_V | 404_L | 1.123 | 0.92 |
| 223_W | 307_A | 1.120 | 0.92 |
| 56_A | 59_T | 1.117 | 0.92 |
| 157_Q | 161_D | 1.113 | 0.91 |
| 226_F | 337_T | 1.107 | 0.91 |
| 371_K | 488_N | 1.105 | 0.91 |
| 322_D | 330_G | 1.105 | 0.91 |
| 31_Y | 82_L | 1.103 | 0.91 |
| 46_I | 87_D | 1.101 | 0.91 |
| 390_V | 400_Q | 1.101 | 0.91 |
| 24_R | 217_V | 1.093 | 0.90 |
| 202_D | 205_T | 1.086 | 0.90 |
| 154_A | 454_E | 1.081 | 0.90 |
| 347_E | 373_T | 1.081 | 0.90 |
| 180_G | 424_L | 1.080 | 0.90 |
| 117_F | 128_C | 1.077 | 0.90 |
| 170_C | 258_D | 1.063 | 0.89 |
| 114_I | 429_M | 1.060 | 0.89 |
| 124_F | 149_L | 1.057 | 0.88 |
| 129_K | 166_Y | 1.056 | 0.88 |
| 23_R | 61_V | 1.053 | 0.88 |
| 269_K | 444_E | 1.051 | 0.88 |
| 236_I | 335_L | 1.050 | 0.88 |
| 349_V | 373_T | 1.047 | 0.88 |
| 373_T | 385_Q | 1.045 | 0.88 |
| 96_T | 134_A | 1.044 | 0.88 |
| 287_Q | 311_S | 1.042 | 0.87 |
| 283_T | 317_V | 1.041 | 0.87 |
| 262_D | 265_V | 1.034 | 0.87 |
| 282_K | 322_D | 1.033 | 0.87 |
| 289_T | 463_P | 1.030 | 0.87 |
| 261_R | 438_R | 1.025 | 0.86 |
| 312_N | 463_P | 1.024 | 0.86 |
| 64_E | 68_T | 1.024 | 0.86 |
| 169_E | 174_R | 1.024 | 0.86 |
| 100_S | 133_E | 1.022 | 0.86 |
| 210_E | 318_A | 1.017 | 0.86 |
| 284_V | 475_A | 1.015 | 0.86 |
| 291_G | 468_A | 1.014 | 0.85 |
| 375_R | 381_G | 1.013 | 0.85 |
| 247_I | 348_V | 1.012 | 0.85 |
| 251_P | 356_E | 1.007 | 0.85 |
| 13_V | 115_N | 1.007 | 0.85 |
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
- Text file of contact predictions (all 3L/2 predictions)
- Generate restraints for ROSETTA Structure Modeling
- See coupling matrix (for protein design or mutagenesis)
- Import to GREMLIN BETA - features includes ability to upload PDB and latest P(contact) calculation based on eLife 2015 fitting to PDB data.
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
| PDB | M | Cov | P(%) | HHΔ | |
| 1dpgA | 2 | 0.9715 | 100 | -0.024 | Contact Map |
| 2bh9A | 1 | 0.9674 | 100 | -0.017 | Contact Map |
| 4e9iA | 4 | 0.9552 | 100 | 0 | Contact Map |
| 4lgvA | 4 | 0.9267 | 100 | 0.02 | Contact Map |
| 3u3xA | 5 | 0.6354 | 96.9 | 0.959 | Contact Map |
| 3rc1A | 1 | 0.6375 | 96.4 | 0.961 | Contact Map |
| 3i23A | 2 | 0.6701 | 96.3 | 0.962 | Contact Map |
| 3f4lA | 6 | 0.6538 | 96.3 | 0.962 | Contact Map |
| 2p2sA | 2 | 0.6456 | 96.2 | 0.962 | Contact Map |
| 1zh8A | 2 | 0.6253 | 95.9 | 0.963 | Contact Map |
Cov = coverage of hit
P(%) = probability
Sequence Conservation (Image Generated using WebLogo v3)