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OPENSEQ.org

glucagon_receptor

ID: 1497819904 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 477 (404)
Sequences: 410 (214.4)
Seq/Len: 1.015
Nf(neff/√len): 10.7

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 1.015).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
401_C404_N3.2721.00
401_C406_E2.9481.00
400_Y404_N2.6301.00
400_Y406_E2.5170.99
324_R327_Q2.1160.97
402_F406_E2.1070.97
63_D67_C2.0960.97
404_N407_V2.0150.96
58_C67_C2.0070.96
160_L178_A1.9340.94
71_T77_A1.8520.93
401_C407_V1.8440.92
322_F347_L1.8210.92
403_L406_E1.7930.91
350_S385_D1.7710.90
401_C405_K1.7350.89
233_Y245_E1.7340.89
58_C63_D1.6970.88
400_Y403_L1.6960.87
400_Y407_V1.6910.87
60_R70_D1.6770.87
286_K292_V1.5980.83
183_S238_N1.5840.82
169_L233_Y1.5790.82
58_C68_W1.5780.82
172_T175_A1.5620.81
241_W245_E1.5490.80
146_T195_D1.5220.79
242_L322_F1.4900.76
231_M358_L1.4860.76
163_L278_F1.4840.76
148_G152_S1.4820.76
246_G250_H1.4650.75
97_F120_Q1.4430.73
170_H174_N1.4210.72
245_E271_G1.4190.71
225_R291_N1.4160.71
174_N282_W1.4030.70
145_Y176_I1.4020.70
350_S382_L1.3990.70
400_Y405_K1.3930.69
187_K194_I1.3890.69
192_L350_S1.3850.69
322_F350_S1.3840.69
393_G404_N1.3620.67
66_S79_I1.3610.67
173_R177_H1.3590.67
245_E272_W1.3560.66
322_F361_H1.3510.66
347_L350_S1.3450.65
170_H270_I1.3420.65
402_F407_V1.3330.64
245_E249_L1.3300.64
172_T176_I1.3210.63
171_C184_F1.3170.63
163_L398_V1.3140.63
393_G406_E1.3070.62
47_L60_R1.3030.62
69_P286_K1.2970.61
148_G156_L1.2950.61
240_C270_I1.2900.61
238_N361_H1.2900.61
67_C82_P1.2820.60
319_F391_F1.2760.59
168_K174_N1.2760.59
224_C245_E1.2600.58
176_I229_V1.2560.58
281_P285_V1.2540.57
71_T94_R1.2510.57
242_L350_S1.2440.56
305_W312_F1.2410.56
244_V282_W1.2320.55
233_Y241_W1.2310.55
410_E415_W1.2310.55
246_G270_I1.2280.55
325_I333_L1.2260.55
347_L386_L1.2210.54
361_H399_L1.2210.54
357_L397_A1.2180.54
304_W308_R1.2160.54
184_F351_T1.2140.54
307_L311_V1.2090.53
148_G181_F1.2090.53
413_R416_H1.2080.53
177_H180_L1.2040.53
81_C392_Q1.2040.53
167_S391_F1.2030.53
63_D68_W1.2030.53
168_K322_F1.2030.53
155_A398_V1.2010.53
246_G271_G1.1980.52
172_T183_S1.1960.52
322_F410_E1.1910.52
174_N187_K1.1850.51
273_G400_Y1.1820.51
225_R351_T1.1800.51
233_Y272_W1.1790.50
155_A394_L1.1780.50
323_V334_R1.1730.50
74_N186_L1.1710.50
264_F267_Y1.1660.49
184_F187_K1.1650.49
232_Q237_A1.1630.49
193_V313_L1.1610.49
168_K185_V1.1590.49
236_V415_W1.1590.49
294_C310_P1.1590.49
352_L356_P1.1580.49
396_V404_N1.1560.48
189_S193_V1.1530.48
279_V283_A1.1500.48
167_S307_L1.1500.48
193_V201_R1.1490.48
188_A194_I1.1410.47
242_L347_L1.1410.47
355_I386_L1.1380.47
58_C82_P1.1360.46
356_P401_C1.1320.46
59_N151_L1.1320.46
155_A197_L1.1310.46
58_C69_P1.1280.46
350_S410_E1.1280.46
159_A187_K1.1250.45
242_L253_L1.1230.45
185_V397_A1.1140.44
333_L397_A1.1140.44
169_L325_I1.1110.44
238_N241_W1.1080.44
106_W117_D1.1080.44
269_G317_I1.1070.44
403_L407_V1.1070.44
174_N188_A1.1060.44
194_I322_F1.1040.44
171_C175_A1.1040.44
35_K38_L1.1020.43
381_K384_F1.1020.43
252_L286_K1.1000.43
393_G401_C1.0980.43
178_A405_K1.0980.43
158_L182_A1.0970.43
331_A337_Q1.0960.43
253_L346_R1.0960.43
309_F312_F1.0950.43
295_W310_P1.0950.43
98_K187_K1.0920.42
168_K187_K1.0880.42
247_L321_I1.0850.42
296_T350_S1.0850.42
358_L402_F1.0840.42
338_M343_Y1.0840.42
187_K322_F1.0830.42
305_W309_F1.0820.42
243_L252_L1.0790.41
237_A267_Y1.0770.41
325_I332_K1.0760.41
322_F386_L1.0760.41
294_C305_W1.0750.41
63_D69_P1.0740.41
81_C106_W1.0720.41
393_G396_V1.0670.40
306_I418_W1.0600.40
382_L411_L1.0590.39
181_F188_A1.0560.39
396_V406_E1.0550.39
157_L284_V1.0540.39
42_Q287_C1.0510.39
61_T70_D1.0510.39
174_N195_D1.0500.39
58_C62_F1.0470.38
100_C121_C1.0440.38
68_W82_P1.0410.38
225_R362_E1.0380.38
394_L398_V1.0370.38
149_Y390_S1.0360.37
187_K351_T1.0360.37
155_A381_K1.0350.37
245_E248_Y1.0350.37
175_A195_D1.0350.37
248_Y271_G1.0310.37
149_Y159_A1.0280.37
62_F65_Y1.0260.37
97_F267_Y1.0260.37
249_L272_W1.0260.37
173_R180_L1.0250.36
250_H327_Q1.0240.36
195_D322_F1.0240.36
242_L328_L1.0230.36
249_L271_G1.0220.36
61_T414_R1.0210.36
347_L410_E1.0190.36
162_I380_A1.0180.36
244_V278_F1.0180.36
146_T163_L1.0170.36
183_S361_H1.0160.36
262_S363_V1.0150.36
321_I358_L1.0140.36
184_F238_N1.0130.35
354_L359_G1.0110.35
335_A399_L1.0110.35
145_Y367_F1.0050.35
244_V270_I1.0050.35
174_N184_F1.0030.35
187_K282_W1.0020.34
63_D82_P1.0020.34
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
4k5yA30.53041000.638Contact Map
4l6rA10.79871000.676Contact Map
2qkhA10.19711000.833Contact Map
4hj0A20.19291000.839Contact Map
3c5tA10.21381000.841Contact Map
4ersA10.20131000.844Contact Map
4jkvA20.76311000.846Contact Map
4rwfA1099.90.853Contact Map
1u34A10.241199.90.859Contact Map
4qinA10.643699.90.86Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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